Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	64 [0]	[0] 63	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	61 (1.460)	47/276	0.0	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

AAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTT  >  NC_000913/1299356‑1299640                                                                                                                                               |                                                                                                                                               aaaTTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAA                                                                                                                                                >  2:260938‑M1/1‑143 (MQ=255)     tACACTTAGTTATAAGGATTTCCTTAACTGCTCCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTCGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataa                                                                                                                                            <  1:189915‑M1/143‑5 (MQ=255)        agttagttATAAGGATTGCCCTAACTGCTTCTCCTCGCCATCATGTTATTTTCGCAACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataa                                                                                                                                         <  2:527276‑M1/143‑8 (MQ=255)                    tgaTTTCCTGAACTGCTTCTCCTCGCCATCTTGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAAGAATTGGAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaa                                                                                                                             <  2:730823‑M1/142‑20 (MQ=255)                    ggATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaa                                                                                                                             <  2:503643‑M1/143‑20 (MQ=255)                         tCCTTATCTGCTTGTCCTGGCCATGATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                                                                                                                        <  2:775889‑M1/143‑25 (MQ=255)                         tCCTTAACTGCTTCTCCTCTTCATTATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                                                                                                                        <  1:79805‑M1/143‑25 (MQ=255)                           cTTAACTGCTTCTCCTCGTCATGAGGTTAGTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgt                                                                                                                      <  2:505645‑M1/143‑27 (MQ=255)                           cTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAGTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgt                                                                                                                      >  1:51880‑M1/1‑117 (MQ=255)                           cTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAatattaatcaatagttaaattatggt                                                                                                                      >  1:564348‑M1/1‑117 (MQ=255)                           cTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgt                                                                                                                      <  1:624300‑M1/143‑27 (MQ=255)                              aaCTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgca                                                                                                                   <  1:420304‑M1/143‑30 (MQ=255)                              aaCTGCTTCTCCTCACCATCATGTTATCTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgca                                                                                                                   <  1:508022‑M1/143‑30 (MQ=255)                               aCTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcat                                                                                                                  >  1:75373‑M1/1‑113 (MQ=255)                                   cTTCTCCTCACCATCATGTTATTTTCGCCACATCCTAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttca                                                                                                              <  1:543939‑M1/143‑35 (MQ=255)                                   cTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttca                                                                                                              <  1:201697‑M1/143‑35 (MQ=255)                                      ctcctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacta                                                                                                           >  2:94969‑M1/1‑106 (MQ=255)                                      ctcctcACCATCATGTTATTTTCGCAACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCAACTGCCTTTGGTATAGGAAAGTGCTAAataataatcaatggttgaattattgggcaattcagtg                                                                                                           >  2:635431‑M1/1‑106 (MQ=255)                                       tcctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactac                                                                                                          >  2:75274‑M1/1‑105 (MQ=255)                                         ctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactg                                                                                                        >  1:183785‑M1/1‑103 (MQ=255)                                          tcgccatcATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgg                                                                                                       <  2:484863‑M1/140‑42 (MQ=255)                                          tcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgg                                                                                                       >  1:132862‑M1/1‑102 (MQ=255)                                             ccATCATGTGATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAAAAATAGAAAGTACCTGATTTTTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaac                                                                                                    <  1:359420‑M1/143‑45 (MQ=255)                                              catcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaact                                                                                                   >  1:781621‑M1/1‑98 (MQ=255)                                                tcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgt                                                                                                 >  2:499893‑M1/1‑96 (MQ=255)                                                   tggtattTTCGCCACATCATAATACTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                              <  2:185766‑M1/140‑51 (MQ=255)                                                   tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                              <  1:464287‑M1/143‑51 (MQ=255)                                                             gCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGACATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagat                                                                                    <  1:550389‑M1/143‑61 (MQ=255)                                                                acaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTACCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatatgaaaagaaaga                                                                                 >  2:485‑M1/1‑80 (MQ=255)                                                                  atcatAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAAAGGAACATGCAAAataataatcaattcttaaattattgtgcatttcactaatggaactgtaatcagaaaagatagaca                                                                               >  2:50875‑M1/1‑78 (MQ=255)                                                                    catAATCCTGGGCTAGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcaaaaaagatagacatg                                                                             >  2:209500‑M1/1‑76 (MQ=255)                                                                         tCCTCGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttag                                                                        <  1:476617‑M1/143‑73 (MQ=255)                                                                                   gCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctc                                                              >  1:498213‑M1/1‑61 (MQ=255)                                                                                    cTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctat                                                             <  2:592240‑M1/143‑84 (MQ=255)                                                                                    cTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCGTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttgacaactggaactgtaatcagaaaagatagacatgcttacccaatctctat                                                             >  2:309001‑M1/1‑60 (MQ=255)                                                                                     tGAAGAATAAGTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatt                                                            <  2:362211‑M1/143‑85 (MQ=255)                                                                                       aagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctta                                                                         >  1:744669‑M1/1‑57 (MQ=255)                                                                                       aagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctta                                                                         <  2:744669‑M1/128‑72 (MQ=255)                                                                                               ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagacaatctctattagattggatt                                                  >  2:156285‑M1/1‑49 (MQ=255)                                                                                                 gAAATTATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgcgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattga                                                <  1:457616‑M1/143‑97 (MQ=255)                                                                                                 gAAATGATATTATTAATTCCACTTCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattga                                                <  2:492320‑M1/143‑97 (MQ=255)                                                                                                 gAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattga                                                <  2:694019‑M1/143‑97 (MQ=255)                                                                                                  aaaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaa                                               <  1:552782‑M1/143‑98 (MQ=255)                                                                                                      gATATAATTAACTCCTCTGCCTTTGGTTTAGGAAAGTGCTAAataataatcaatttttaaattattgtgcatttcactactggaactttaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagat                                           <  2:435377‑M1/143‑102 (MQ=255)                                                                                                         attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttcgccaatctctatttgattgaattgaaacttgtt                                        >  2:105157‑M1/1‑39 (MQ=255)                                                                                                            attaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgt                                     >  1:557625‑M1/1‑36 (MQ=255)                                                                                                            attaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgt                                     <  1:517282‑M1/143‑108 (MQ=255)                                                                                                            attaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgt                                     >  2:54349‑M1/1‑36 (MQ=255)                                                                                                            agtaatTCCACTGTCGTTGGGAGAGGAAAGTGCTAGataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgt                                     <  1:348973‑M1/141‑108 (MQ=255)                                                                                                              taATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaggatgtttgtta                                   >  2:120279‑M1/1‑34 (MQ=255)                                                                                                                  tCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaacctctatttgattgaattgaaagatgtttgttaaggc                               <  1:257885‑M1/143‑114 (MQ=255)                                                                                                                    cACTGCCTTTGGTAGAGGAAAGAGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcat                             <  2:356118‑M1/143‑116 (MQ=255)                                                                                                                     aCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatg                            <  1:664751‑M1/143‑117 (MQ=255)                                                                                                                      cTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaagtattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatgg                           <  1:557638‑M1/143‑118 (MQ=255)                                                                                                                        gCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggat                         <  1:430173‑M1/143‑120 (MQ=255)                                                                                                                        gCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggat                         <  2:189071‑M1/143‑120 (MQ=255)                                                                                                                        gCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtacatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggat                         <  2:403589‑M1/143‑120 (MQ=255)                                                                                                                          cTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgc                       >  1:113884‑M1/1‑22 (MQ=255)                                                                                                                            ttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaa                     <  2:388460‑M1/143‑124 (MQ=255)                                                                                                                                tAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagcta                 <  1:260938‑M1/143‑128 (MQ=255)                                                                                                                                      aaaGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagatt           <  1:47364‑M1/143‑134 (MQ=255)                                                                                                                                       aaGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaagctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattc          <  2:480605‑M1/143‑135 (MQ=255)                                                                                                                                            cTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatgaatgcaagctatatattctgata     >  2:765033‑M1/1‑4 (MQ=255)                                                                                                                                               aataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagctgtttgttaaggcacggatgcaagctataaattctgatacgg  >  1:654893‑M1/1‑1 (MQ=255)                                                                                                                                               |                                                                                                                                               AAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTT  >  NC_000913/1299356‑1299640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑