Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,395,466 G→C 13.0% A144G (GCT→GGT)  nuoJ ← NADH:ubiquinone oxidoreductase, membrane subunit J

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,395,4660GC13.0% 165.9 / 3.3 58A144G (GCT→GGT) nuoJNADH:ubiquinone oxidoreductase, membrane subunit J
Reads supporting (aligned to +/- strand):  ref base G (43/7);  new base C (2/6);  total (45/13)
Fisher's exact test for biased strand distribution p-value = 9.27e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAA  >  NC_000913/2395232‑2395698
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                        
cATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAg                                                                                                                                                                                                                                      >  1:156669/1‑239 (MQ=255)
  tGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGTCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGt                                                                                                                                                                                                                                     >  1:1208372/1‑238 (MQ=255)
   gATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGtt                                                                                                                                                                                                                                    >  1:136870/1‑238 (MQ=255)
                aTCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACg                                                                                                                                                                                                                      >  1:173743/1‑239 (MQ=255)
                aTCAACATAAACAGCAGATTGCGACGGATAACCAGACCGCTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGACCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCCCCCGCACGCTCTTCACGACCGACGTGGACGGCCACAACCAGACCTGCGAGCAGCAGCATAGAACCCAGTTCCACCGCCAGTACg                                                                                                                                                                                                                      >  2:631049/1‑239 (MQ=255)
                          aCAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGa                                                                                                                                                                                                            >  1:1010194/1‑239 (MQ=255)
                             gcagATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACa                                                                                                                                                                                                         >  1:782016/1‑239 (MQ=255)
                             gaagtgtGCGAGTGAAAACCGCACCGTGTAAGCCAGGAAAGAATAAGATCGCCGCTATGATCGCACCTGTGTGTCGGGGCATCGTGCGTGCCCCCCCTTTTTGCTTTCCGCGCTGCCTTCTTTACGATTGCTCACCACTCCCCCCGCCAGCTCTCCACGACCGCCGTGGAGGCCCACACCCAGTCCTGCGAGCAGCAGCAGAAAACCCAGTTCCACCGCCAGTACGTAAGGCCCGaa                                                                                                                                                                                                           <  2:667136/231‑1 (MQ=255)
                              gaagtggCGACGGATAACCAGACGGATTAAGCAAAGAAGGAATAAGGTGCCCGCGGGGTTCAGTCAAGGTTGTAAGGGGTTCATCCGGGCTCCCCCGTTTTTCTTTCCCCGCGTCCGTCTTTAGATTTGCCCAGCCCTCCCCCCGCACGCTTTTCACGACCGCCGTGGAAGCCCACACCCAGCCCTGCGAGCAGCAGCAGAGATGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACa                                                                                                                                                                                                         <  2:702751/232‑1 (MQ=255)
                              cagATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGACGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACa                                                                                                                                                                                                         >  1:41678/1‑238 (MQ=255)
                               agATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGc                                                                                                                                                                                                       >  1:1017171/1‑239 (MQ=255)
                                 aTTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGt                                                                                                                                                                                                     >  1:2698/1‑239 (MQ=255)
                                 aTTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGt                                                                                                                                                                                                     >  1:1128939/1‑239 (MQ=255)
                                           atacccgtcccGGTTAACCAAAGACCGATTAAGTTTCCCGCGATGATCATTCCTTTTTGTAAGGGGATCATCCCTCCTCCCCCTTTTTTTTTTCCCCGCGTTCGTCTTTCCGATTGCACAGCACTCCCCCCCCCCGCTCTCCACGACCGCCGGGAAGGCCCACACCCAGCCCGGCGAGCAGCAGCATAAAACCCGGTCCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCAc                                                                                                                                                                                            <  2:1172974/229‑1 (MQ=255)
                                           aTACCCAGACGGGTGAAGCCAAGAACGAATAAGATCGCGGCGATGTTCAGTCCGTGTTGTAAGGGGATCATGCGTCCCCCTCCTTTTTTCTTTCCGCGCTGTCGTTTTTACGATTGCCAACCACTCCCCCCGCACCCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCAc                                                                                                                                                                                            <  2:173743/238‑1 (MQ=255)
                                           aTAACCAGGCGTGTTAAGCAAAGAACAAATAAGTTCCCCGCGGGCATGTGTCCGTTTGGTAAGGGGATCCGGCTCGCCCCCCCTTTTTTCTTCTCGGGCTGCCGTCTTCACGATTGCCCAGCACTTCCCCCGCACCCTCTACACGCCCGAGGTGGACGGCCACAACCAGCCCGGCGAGCCGCAGCATAAAACCCAGTCCCGCCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCAc                                                                                                                                                                                            <  2:1208372/238‑1 (MQ=255)
                                                   aCCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGc                                                                                                                                                                                   >  1:660119/1‑239 (MQ=255)
                                                    ccGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGc                                                                                                                                                                                   >  1:150935/1‑238 (MQ=255)
                                                    ccGGTGAAGACAAGGACAAAGAATACTGCCGGGGTGATTAGTCCATTGTGTGCGGGTACCGTGCGTCCTCCTCTCTTTTTCTTTTCGCTCTGTCTTCTTTACGATTGCCCAGCACTTCGCCCGCCCGCTCTTCCCGCCCGACGTCAAAGACCACAACCAGCCCTGCGGGCAGCAGCATAAAAGCCAGTTCCGCCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCa                                                                                                                                                                                  <  2:859170/239‑1 (MQ=255)
                                                                 aaaagAATAAGTGTCCCGCGGGGCTCAGTCGATTTTGTGAGGGGACCAGGCGTGCCCCCCTGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCACAGCCCCCCCCCCGCACGCTCTTCACGACGGACGGGAAAGGCAACACCCAGACCGGCGGGAAGCAGCAAAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTAc                                                                                                                                                                     <  2:136870/235‑1 (MQ=255)
                                                                       aTAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGaa                                                                                                                                                                                                           >  1:930788/1‑195 (MQ=255)
                                                                              tGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTg                                                                                                                                                        >  1:904913/1‑239 (MQ=255)
                                                                                ccGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGa                                                                                                                                                       >  1:258300/1‑238 (MQ=255)
                                                                                ccGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGa                                                                                                                                                       >  1:308735/1‑238 (MQ=255)
                                                                                     aGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCa                                                                                                                                                                                  >  1:1021825/1‑206 (MQ=255)
                                                                                               cctgttttTAGGGGGTCAGTGCGTCCCCCTCCTTTTTTCTTTCCGCGCTGCCGTTTTACCGTTTACCCACCACTACCCCCGCCCGCTCCACCCGACGGACGGGGAGGGCCACAACCAGCCCGGCGAGCACCAGCATAAAACCCAGTCCCTCCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAgg                                                                                                                                        <  2:1017171/237‑1 (MQ=255)
                                                                                                  gttgtAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATg                                                                                                                                     >  1:980218/1‑238 (MQ=255)
                                                                                                  gttgtAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAACCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTCACTGGCGTACCGTCGATACCCTGATCCTTAACACCGACGATg                                                                                                                                     >  2:868722/1‑238 (MQ=255)
                                                                                                              accATGCGTGCTCCTCCTTTTTTCTTTTCGCGCTGTCGTCTTTACGTTTGCTCAGCACTACCCCCGCACGCTCTACACGACCGACGTGGAAGGCCAAACCCAGCCCGGCGAGAAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAAt                                                                                                                          <  1:100189/236‑1 (MQ=255)
                                                                                                              aTCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCa                                                                                                                                                                                  >  1:408568/1‑182 (MQ=255)
                                                                                                              aTCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCAAACAGCGTAATACACACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATAGTTAACACCGAGGATGGCGTAAACAATc                                                                                                                         >  1:55143/1‑239 (MQ=255)
                                                                                                                cATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATca                                                                                                                        >  1:406283/1‑238 (MQ=255)
                                                                                                                      gTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCAccacca                                                                                                                  >  1:59753/1‑239 (MQ=255)
                                                                                                                         ctcctcCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCa                                                                                                               >  1:318426/1‑239 (MQ=255)
                                                                                                                         ctcctcCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCa                                                                                                               >  1:1130953/1‑239 (MQ=255)
                                                                                                                                      cttttcGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTgg                                                                                                                                                                          >  1:622941/1‑167 (MQ=255)
                                                                                                                                         ttcGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAAt                                                                                               >  2:994059/1‑239 (MQ=255)
                                                                                                                                                          aCGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGACTGGCGTAAACAATCACCACAAGCATGATGGCCGACAAAATTGCCGCACCAATCcac                                                                               >  1:1004757/1‑238 (MQ=255)
                                                                                                                                                            gATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCcaaa                                                                              >  1:262866/1‑235 (MQ=255)
                                                                                                                                                               tGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGGAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTACCGGACCAATCCACACCTg                                                                          >  2:1104484/1‑238 (MQ=255)
                                                                                                                                                                 cTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTACTACCCACTGCTTTAGCACTGATTGGCGTACCGTCGCTACCCCGCTCGTTAACACCGAGGATGGTGTAAACAATCACCACCAGCATGATGGCCGAAAAACTTGCCGGACCAATCCAAACCTCCgg                                                                       >  2:380718/1‑239 (MQ=255)
                                                                                                                                                                              ccGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGACCAGCGTAATACCCACTGCTTTAGCACTGATTGGCGCACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGCTGGCCGACAAAATTGCCGGACCAATCCCCACCTGCGGTCTCAGcacccg                                                           >  2:1203077/1‑233 (MQ=255)
                                                                                                                                                                                      tctTCACGACCGACGTGGAAGGCCCCAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGtt                                                      >  1:173067/1‑235 (MQ=255)
                                                                                                                                                                                        ttCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGt                                                 >  2:77068/1‑238 (MQ=255)
                                                                                                                                                                                        ttCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGGACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCCATCCACACCTGCGGTTTCAGCCACTGACGTTCCTGt                                                 >  2:409255/1‑238 (MQ=255)
                                                                                                                                                                                         ccacacgcggcGTGGAGGGCCACAACCAGCCCGGCGACCCACAGCATGGAACCCATTGCCACCGCCAGTCAGGAAGGCCCGAACAGCGTAATTCCCACTGCTTTAGCACGGATTGGCGTCCCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAGTCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTc                                               <  2:104982/229‑1 (MQ=255)
                                                                                                                                                                                                cgacGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGAtt                                           >  1:1100084/1‑236 (MQ=255)
                                                                                                                                                                                                  cggTGAAAGGCCCCAACCAGCCCTGCGACCGGCAGCATAGACGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGa                                      <  1:1064587/237‑1 (MQ=255)
                                                                                                                                                                                                  acGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTACTACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCAACACCTGCGGTTTCAGCCACTGTCGGTCCTGTTCGATTTCTGa                                      >  1:859403/1‑239 (MQ=255)
                                                                                                                                                                                                  acGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGCCGTTCCTGTTCGATTTCTGa                                      >  2:656296/1‑239 (MQ=255)
                                                                                                                                                                                                              cacaACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGt                           >  1:128640/1‑239 (MQ=255)
                                                                                                                                                                                                                caACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATAGTTAACACCCAGGATGCCGTAAACTATCCCCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCCGCGGTTTCAGCCACTGGCCTTCCTGTTCGCTTTCTCAACCGCCCAGGTTg                         >  2:997903/1‑239 (MQ=255)
                                                                                                                                                                                                                           gCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCAtcatca               >  1:229544/1‑238 (MQ=255)
                                                                                                                                                                                                                           gCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCAtcatca               >  1:833254/1‑238 (MQ=255)
                                                                                                                                                                                                                             gAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGCTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATcacc             >  2:828805/1‑238 (MQ=255)
                                                                                                                                                                                                                                 aggtgcaTAGAACCCATAGCAACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACcacga         <  1:58747/234‑1 (MQ=255)
                                                                                                                                                                                                                                 agcagcaTAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACcacga         >  2:686434/1‑238 (MQ=255)
                                                                                                                                                                                                                                       aTAAAAGCCAGTCCCGCCGGCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACcacgaacacgaa  <  1:985454/239‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                        
CATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAA  >  NC_000913/2395232‑2395698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: