Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,436 C→A 14.6% intergenic (‑180/‑27) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4360CA14.6% 171.2 / 5.1 76intergenic (‑180/‑27)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (17/44);  new base A (2/9);  total (23/53)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTG  >  NC_000913/3206199‑3206670
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   
tgtgAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCa                                                                                                                                                                                                                                                    <  1:860752/239‑1 (MQ=255)
   gAACGATTTCTCCCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGa                                                                                                                                                                                                                                                  <  1:1100891/238‑1 (MQ=255)
     aCGATTTCTACCAGTAACTGCAAGTATTTGGTTAAAGGGCAGCTATTGAGCATCTTTAAATATCCGCGGTTGTGATAAGCACAGTGGATTACGCATAACATTGCTATGCCCGGCTGTGGCGAAATATACATTCTTATTTTCGCGTGGATTCATGAACTGTTAAGTAGAAATACACGGTGGCAAAAAATATAAAACCACATTTGTGGTGGTAGTTTGTGGCGGTGAATTTTTCCAGGCa                                                                                                                                                                                                                                                <  2:971240/238‑1 (MQ=255)
       taTTTCTACCAGTACCTGCAAGTTTGTGGCTAAAGGCCAGCTATTCAGCAACGTTAATTATTCGCAGTTGTGATAAGCGCAGTGTATGTCGCAAAACATTGCCATTCTCTGCTCTGGCGAAATATTCATTCTTATTTTCGAGTTGATTCACGAACTGTTAATTACATAGTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATGTTTCCAGACAAAt                                                                                                                                                                                                                                             <  2:989636/238‑1 (MQ=255)
         ttgcTACCAGTACACTCAAGTCTTTGGCTAAAGGCCATCTATTCAGCAGCGTTAATTATCCGCAGTTGTGATAAGCTCAGTGTATTACGCAAAACATTGCCATTCCCTGCGCTGGCGCAATAGTCATTCTTATTTTCGCGTTGATTCACGAACTGGTAATTACATATTCACGGTGGCAAAAAATATAAACCCACAATTTTAGTGGTAGTTTGTGGCGGTGAATTTTGCCAGACAAATAc                                                                                                                                                                                                                                           <  2:231933/236‑1 (MQ=255)
              aCCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAaaaaaa                                                                                                                                                                                                                                     >  1:322679/1‑239 (MQ=255)
                   tACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAcagta                                                                                                                                                                                                                                >  1:401234/1‑235 (MQ=255)
                    aacTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTgg                                                                                                                                                                                                                               <  2:559462/235‑1 (MQ=255)
                    aCCTGCAAGTCTTTGGCTAAATGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                                                                                                                            <  1:426195/239‑1 (MQ=255)
                    aCCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                                                                                                                            <  2:227431/239‑1 (MQ=255)
                                     tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGGTGAGCGa                                                                                                                                                                                                          <  1:681629/238‑1 (MQ=255)
                                     tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                          <  1:1212992/238‑1 (MQ=255)
                                            cAGCTATTTAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCATTGTATATCGCAAAACAAGGCCATTCCCAGCCCTGACGCAATATTCATTCTTATTGTCTCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATATTTAGTGGTAGTTTGTAGCGGGGAATTTATCCAGACAAATACAAAAACTGGAGTTGCCAGGATGAGCGAATGtaat                                                                                                                                                                                                   <  2:475181/238‑1 (MQ=255)
                                            cAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                   <  2:322679/238‑1 (MQ=255)
                                              gCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACCAAAAAAAAAAATAGGGTTGCCCTTATGAGCGAAAGgaataaa                                                                                                                                                                                                >  1:117852/1‑238 (MQ=255)
                                                    cAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAAATGGGTTGTCCTGATGTGCGCAAAtaaaaaaaaaaaa                                                                                                                                                                                          >  1:353338/1‑232 (MQ=255)
                                                       cATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTACACAAATAAAAAAAATGGAGTTGTCATGTTGAGGGAAAGtaaaaaaaaaaagaca                                                                                                                                                                                       >  1:609553/1‑229 (MQ=255)
                                                        aTCGTTAATTATCCGCATTTGTGATAAGCGCAGTGTATTTCGCAAAACATCGCCATTTCCGTCTCTGGCGTAATATTCATTCTTATTTTAGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACATAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                                                                                                                        <  2:418246/237‑1 (MQ=255)
                                                        aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                         <  1:245308/236‑1 (MQ=255)
                                                        aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                         <  1:466107/236‑1 (MQ=255)
                                                        aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                         <  2:679385/236‑1 (MQ=255)
                                                        aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                                                                                                                        <  1:405568/237‑1 (MQ=255)
                                                        aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                                                                                                                        <  1:527421/237‑1 (MQ=255)
                                                        aTCGTTAATTATCCGAAGTTGCGATAAGCGCAGTGTATTTCGCAAAACATTGTCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                         <  2:884321/236‑1 (MQ=255)
                                                             tAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCTATATTCAGTAGTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTGCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                                                                                                                  <  2:362577/238‑1 (MQ=255)
                                                                tgctCCGCAGTTCGGATACGCGCAGTGTATTTCTCAAAACATTGCCATTACCTGCTTTAGCGCAATATTCATTCTTATGTTCATGTTGATTTACGAACTCTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGGTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGTCACTGAATAAg                                                                                                                                                                              <  2:165387/236‑1 (MQ=255)
                                                                     cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTATAAAAATAAAAAAAATGGAGGTGTCATGGTGAGGGGTAGTAATAAACAACAAGCAATGAACAAGTGGAc                                                                                                                                                                         >  2:42500/1‑239 (MQ=255)
                                                                      gCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATATTTTCAGACAAATACAAAAAATGGGGTTTCCAGGGTTGGCGAAAGATAAAAGCAAAACGCAGTGAATAAATTGGCa                                                                                                                                                                        >  1:766827/1‑239 (MQ=255)
                                                                           tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                                                                                                                    <  1:849748/238‑1 (MQ=255)
                                                                           tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                                                                                                                    <  2:560479/238‑1 (MQ=255)
                                                                           tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                                                                                                                   <  1:457775/239‑1 (MQ=255)
                                                                           tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                                                                                                                   <  1:74784/239‑1 (MQ=255)
                                                                               aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCACACAAAAACAAAAAATTGAGTTGCCATGATGAGAGGAAATAAAAAAAAAAAAGAAATGAATAAGTTTAAAAAAATTGTc                                                                                                                                                               >  2:757226/1‑239 (MQ=255)
                                                                               aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTATACAAAAAAAAAAAATGACGTTGTCCTTAGCAGGGCAAAGTATAAAAAAAAAGCAATGAAAAAGTGTACAGAGATTg                                                                                                                                                                 >  2:13286/1‑237 (MQ=255)
                                                                                  aGCTCAGTTTATTTTGAAAAACATTGCCATTCCCTGCTCTGTCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAATTGTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCACAATTTTAGTGGTATTTTGTGGCGGTAAATTTTTCAAAACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                                                                                                            <  2:609553/239‑1 (MQ=255)
                                                                                  aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                                                                                                            <  1:13286/239‑1 (MQ=255)
                                                                                         tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGATTTTTTCCAGCAAAAACAAAAAATTGAAATTTCCAGATTGGGGGAAGTAATAAAGAACAAGCAAGGAAAAAATTGACAAAGAATTTTGCTAACattt                                                                                                                                                     >  1:1045251/1‑238 (MQ=255)
                                                                                         tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGAATTTTTAAAAAAAAAACAAAAAATGTGATTTGCCAGAGATGAGAGAGGTAAAATACATAAGAAGATAATAAGCTGTCAGAGATTCTTGCTAACttt                                                                                                                                                      >  2:1124421/1‑238 (MQ=255)
                                                                                               tcccAAAACTTCGCTATTCCCGGCTTTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATTTCCGCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTATTTTGGGGGGGTGAATTTTCCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCACCAGGCAGTGAAAAAGTTGACAGAGATTGTCGCTAACTTTACCGCCa                                                                                                                                              <  1:909580/236‑1 (MQ=255)
                                                                                                 acAAAACATTGCCATTCCCTGCTCTGGCGAAATATTAATTCTTATTTTCTCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCCAGACAAATACAAAAACGGGATTTGCCATGATGAGCGAAAGTAATAACCAACAGGCAGTGAATAAGTTGAAAGAGATTGTCGCTACTTTTACCGCCATg                                                                                                                                            <  1:391680/238‑1 (MQ=255)
                                                                                                   aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAAAAATAAAAATTGAGTTTTGCTTATTAGCGTAAATAATAAAAAATACGAAGAGAATAAATTAGAACAGAGTGTCGCTCACCTTATACCCATGAt                                                                                                                                          >  1:854016/1‑239 (MQ=255)
                                                                                                     aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAGACAAAACAAAAAATACAGTTGCCATCATGGGCGGAAGGAATAAGACACAAGCAGTGACAAAGTTGACAAAGATTATTGATAACTTTtcctcccttcttt                                                                                                                                        >  2:915883/1‑232 (MQ=255)
                                                                                                          tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGTAGGTGCCATGATTAGCGAAAGTAATAAAAAACAGGGAGGGAATAAGTTTACAGAGAGTGTGGCTAAATTTACCCCCATGATTTCTAc                                                                                                                                   >  1:1101461/1‑238 (MQ=255)
                                                                                                             taTTCCCTTGTTTGCCGAAATATTCATTCTTATTTTCGCTTTGATTCACGAAATGTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCAAATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTCCCAAACAAATAAAAAAACTGGAGTCGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                                                                                                                               <  1:847011/238‑1 (MQ=255)
                                                                                                              aTTCCCTTCTCGGGCGAAATATTCATTCTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGGGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                                                                                                                              <  1:829109/239‑1 (MQ=255)
                                                                                                                 cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                                                                                                                        <  1:1021953/180‑1 (MQ=255)
                                                                                                                       aaaaagcaaaTATTCATTCTAATTTCGGCGTTAATTCCAAACTTGTTAATTACAATTTGAGGGTGGAAAAAAATAAAAAACAAAATTTTAAGTGGTAGTTGGTGGGGGTGAATTTTTCAAAAAAAAAAAAAAAACGGGAGTGGCCATGAGGAGCGAAAGTAATAAGCAACAGGTAGTGAATAAGTTGACGGAGATTGTCGTTACCTTTACCGCCATGATTTCTACCCGAATGCCtgatg                                                                                                                     <  2:339379/231‑1 (MQ=255)
                                                                                                                                 tataCATTCTTATTTTCGCGTTAATTCACGACCTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTACCAGAAAAATACAAAAACTGGAGTTGCAATGAGGAGCGAAAGTAATAAGCAACAGGCAGTGAAAAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                           <  1:166732/239‑1 (MQ=255)
                                                                                                                                 aattcactcTTATTTTCGGTTTGATACACAAAGTGTTAATTACATATGCACGGTGGAAAAAAATAAAAAACAAAATTTTGAGGGGTAGTTTGTGGGGTAGAATTTTTCCAGAAAAAAAAAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACGGGCAATAAATAAGTTGACAGATATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                           <  2:125645/238‑1 (MQ=255)
                                                                                                                                         cTTATTTTCGTGTTTATTCACGAACTGTAAATTAAATATTCACGGTGAGAAAAAAAATAAAACCAAATTTTGAGGGGGAGTTTGTGGCGGTGAATTTTTCAAGAAAAAAACAAAAACTGGTGTTGCCATGATGAGCGAAATTAATAAGCAACAGGGAGTGAATAAGTTGAAAGAGATTGTCGATAACTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                   <  1:608541/239‑1 (MQ=255)
                                                                                                                                         cTTATTTACGGATTGATCCATGATCTGTTAATTAAATATTAAGGGTGAAAAAAAATAAAAAACCACATTTTAAGTGGAATTTAGTGGCGGTGAATTTTACCAGAAAAAAAAAAAACCGGGATTTGCAATGATGAGCGAAAGTAATAACCAACAGGTAGTTAAAAAGTGGAAAGAGATTGTCGTTACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                   <  2:529126/239‑1 (MQ=255)
                                                                                                                                         cTTATTTACGCTTTAATTCACGAATTGTTAATTACATATGCACGGTGGCAAAAAATAAAAAACCACATTTTTAGGGGTAGTTTGTGGCGGTAAATTTTCCCAGACAAATACAAAAACTGGAGTTGCCATAATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAATTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                   <  1:148751/239‑1 (MQ=255)
                                                                                                                                          ttATTTTCGTGTTAATCCACAAACTGTTAATTACATTTTCACGGTGGCAAAAAATAAAAAACAACATTTATAGGGGTGGTTTGTGGCGGTAAATTTTCCAAAAAAAATACAAAAACGGGAGTTCCAATAAGGATCAAAATAAATAAGCAACAGGCATTAAATAAGTGGAAAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                  <  2:161297/239‑1 (MQ=255)
                                                                                                                                           tATTTTCGCGTTGATTCACGAACTTTTAATTACATATCCCCGGGGGAAAAAAATAAAAACCCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCAAGACAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                 <  1:42500/239‑1 (MQ=255)
                                                                                                                                           tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTATACAAAAACAAAAACTTGAGTTGCCATTGTGTGGGTAACGAAAAAAAAAAAAGCACAGAAAAAATTTGAAAAGATTTTGCATAACTTTTACGCCCATATTTCTACCCGAATGCCTCGTGAAGCGGTGGGTAAACTaaa                                                                                                  >  2:702996/1‑238 (MQ=255)
                                                                                                                                           tATTTTCGCGTTGATTCAAGAACTGTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCAAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                 <  1:154220/239‑1 (MQ=255)
                                                                                                                                                                    gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAACAAAAAAAGGAGTTTGCATTGTGGGGGAAAGGAAAAAAAAAAAAGGAAGGAAAAAATTGACAGAGATTTTCGCTAACCTTAACCCCAAGATTTTTTACCGAATGACTGGAGACGGGGGGGGTAAAAAAAAAAAACTTAAAGAACCCGaaaaggcc                                                                         >  2:25389/1‑234 (MQ=255)
                                                                                                                                                                        attaattatTCAGGGGGAAAAAAAATATAAAACAACATTTATAGTGGTAGTTTGTGGGGGTAAATTTTACCAGACAAATACAAAAACGGAAGTTGCCATGATGAGGAAAAGTAATAAGCAACAGGCAGTTAATAAGTTGACTGAGATGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGa                                                                    <  1:344051/233‑1 (MQ=255)
                                                                                                                                                                         ttAAATATCCACGTTGCAAAAAATAATAAAACCACTGTATTAGTGGTAGTTTGTGTCGGTGAATTATGCCAGAAAAATACAAAAACGGGAGTTTCAAGGATGAGCAAAATTAATAAGCAACAGGAAGGTAATAGGTTGACAGAGATTGTCGATATTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAt                                                                   <  2:322415/239‑1 (MQ=255)
                                                                                                                                                                              tgttcggggTGGCAAAAAATAAAAAACCAAATTGTTAGTGGTAGTTTGTGGGGGTGAATTTTCCCAAAAAAAAACAAAAACTGGTGTTGCCAGAATGAGGAAAATTAAAAAGCAACGGGCAGTGAATAGGTGGACAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                              <  2:1045251/232‑1 (MQ=255)
                                                                                                                                                                              tatTCAGGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTAATTTTTTCCAGACAATTAAAAAAACAGGAGTGGCCATGAGGAGGAAAAGTAAAAAGAAACAGGCGGGGAAGAAATGTAAATAGGTGGCCGGAAGCTTCACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                              <  1:109395/239‑1 (MQ=255)
                                                                                                                                                                               atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATTGAGTTTCCATGATGAGCGGAAAGAATAAACAAAAAGCAGTGAAAAAGATTACAGAGATTGTCGCTACATTTACCCCCATGATTTCTACCCGAAAGCCTGATGACGTGGTGGAGAAACTAAAAAAACTAAAAGAAGCCGGAACATCGTCGATggggga                                                             >  1:958632/1‑237 (MQ=255)
                                                                                                                                                                               atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAAATTGAGTTGCCATGATGAGCGCAAGTAATAAACAACAAGCAGGGGAAAAAATGACAGAGAGTTTCGCTAAATTTACCGCCATGATTTTTACCCGAATACCTGATGAAGGGGGGGGTAAACTAAAAAAACTAAAGGATGCCGAAAACACGTCGGTGGGGaa                                                             >  1:1101168/1‑239 (MQ=255)
                                                                                                                                                                               atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCTGACAAATAAAAAAAATGGGGGTGTCATTGAGGGCGGAAGGTAAAAACAAAAAGAAAAGAATAAATTTATAGAGAACGTCGCTAACTTTACACCCCAGATTTTTTACCCAAATGCTGAGGCAGGGGTGGGTAAAATAAAAAAAATTAAAGATATCCAAAAGTCGTCGAtatggga                                                             >  2:726296/1‑233 (MQ=255)
                                                                                                                                                                               atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAACAAAAAATGGAGTTGGCATGGTGAGCGAAAGTAATAAACAAAAAGCAGAGAAAAAAATGATAGAGATTGGCTGTAAATTTACCACCACGATTTTTACCCGAAGACCAGATGACGCGGGGGATAAACAAAAAAAAACAAAAAATTCCGACAcgtcgtacaggggggg                                                             >  2:966271/1‑229 (MQ=255)
                                                                                                                                                                                   aCGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTGGTGGCGGTGAATTTTCCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAtt                                                         <  1:757226/239‑1 (MQ=255)
                                                                                                                                                                                   aCGGTGGAAAAAAATAAAAACCCAAATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCAAAAAAAATAAAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGAAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAtt                                                         <  1:470818/239‑1 (MQ=255)
                                                                                                                                                                                     ggtgaaaaaaaaTAAAAAACCACATTTTTAGTGGTAGTTGGGGGCGGTGAATTTTCCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                       <  1:25389/233‑1 (MQ=255)
                                                                                                                                                                                      ggggCAAAAAATATAAAACCATATTTTTGGGGGTAGGTTGGGGCGGTGAATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGAGGAGGGAAAGTAATACGCAAAGGGTAGTGAATAGGTTGACAGAGATTGTCGTTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                       <  1:164518/236‑1 (MQ=255)
                                                                                                                                                                                       tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATTGAGGTTGCCTTGGTGGGGGAAATAAAAAAAAAACAGCAAAGAATAAGGTGAACAAGATTTTCCCTTAATTTAACACCAATATTTTTAACCCGATGCCCTGTTACGGTGTGGGAAAAACAAAAAAAGTCAAAGAAACCCAAAACGCCGGCGTGGGGGAAATTAttt                                                     >  2:285026/1‑237 (MQ=255)
                                                                                                                                                                                             aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAGGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATa                                               <  1:1124421/239‑1 (MQ=255)
                                                                                                                                                                                               aaTATAAAACCACATTTTTGGTGGTAGTTGGGGGGGGTGAATTTTTCAAAAAAAATACAAAAACTGGAGTTGCCATGAGGAGCAAAAGTAATAAGCAACAGGCAGGGAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                             <  1:915883/239‑1 (MQ=255)
                                                                                                                                                                                                      aaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAAAAAAAAATGAGGTGGCATGATGGGGGAAAGGAATAAAAAACAAGCAAAAAAAAAAATGAAAGAGAATGTCGCCAACTTTACACCCATGATTTCTACCCCAAAAACTGATGACGGGGGGGATAAACTAAAAAAGTCAAACAGAGCCGCAACATCGTCGGTGGGGGAAATTATATTCCCCACGAAGTTc                                       >  2:350200/1‑239 (MQ=255)
                                                                                                                                                                                                            caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTTGAGGTTTCATGGTGCGACATAATAAAATACAAAAGACATTGAATGAGGTGTAAGAATTTCTAGCTTAAATTACCGCCCTCAGTTTTACCACCACGACTGATGACGTGGTGGATATACTCACACACCTTAATGCGGCCACAACATCGCTCGAGGGGGAAATTATAATCAATACCATGTTCTacaaa                                 >  2:12608/1‑238 (MQ=255)
                                                                                                                                                                                                                      ggTAGTTTGGGGGGGGAAATTTTTCAAAAAAAAACAAAAACTGGGATTTGCAATGATGAGGAAAAGTAAAAAGAAACAGAAAGTGAATAGGTTAAAAGAGATTGCCGTTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaaaa                         <  2:83476/237‑1 (MQ=255)
                                                                                                                                                                                                                          gtgtgggggggTAAATTTTGCAAAAAAAAAACAAAAAGTGAAGTTGCCAGGGTGAGCGAAAGTAATAAGCAAGAGGAAGTAAATAAGTGGAAGGAAATTGTCGTTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                    <  2:353338/229‑1 (MQ=255)
                                                                                                                                                                                                                                             ttcccAGACAAATAAAAAAACTGGAGTTGCCATGGTGAGCGAAATTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg  <  1:538631/236‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   
TGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTG  >  NC_000913/3206199‑3206670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: