| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,560,455 | 1 | . | G | 97.7% | 122.8 / ‑3.0 | 44 | intergenic (‑2/+1) | glpR/glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (20/23); total (20/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01 | |||||||||||
GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACG > NC_000913/3560316‑3560590 | gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGAT‑GGcgcc > 2:285972/1‑140 (MQ=255) aTAAGCGGCGTGGAGGCATTGGGGATTTGTTCCGCGGTTTGGCGGGCGATGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACCGCCATCAT‑GGcggtg < 2:291910/143‑5 (MQ=255) aGCGTCGCGCCATTGCGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGCTCGTGCCACGGCGTGG‑TAACCCAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAg < 1:514891/143‑1 (MQ=255) aGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGGTCAg > 1:639636/1‑143 (MQ=255) ccGACCCACTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGACGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGttt < 1:448229/142‑1 (MQ=255) cGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGCGTAGCCTTGCGATCGTGCCACGGCGTGT‑TGACCGGACTGGAAGGCGGCGCCGCACCGCCACGAT‑GGCGCAGGAGCAgccagc > 2:798913/1‑138 (MQ=255) aTTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTTTTTTTTTTTTTTTTCCTTGCGATCGTGCCACGGCGTGT‑TAACCGTACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCTGGTTTTGCTCCg > 2:618127/1‑143 (MQ=255) ttGGGGATTTGTTGCGCCATTTTGCGGGCGATGCGCTCTTTTGCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGc < 2:612723/143‑1 (MQ=255) ggggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGCTCAGGTTTTGCTCCGCCa > 1:527750/1‑143 (MQ=255) tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACGGGAAGGCAGCGCCGCACAGCGATGAT‑GGCGGAGGATCAGGTTTTGCTCAGCAAGCCCACTgcg > 2:250371/1‑141 (MQ=255) cGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCCCATCGAgga > 2:92884/1‑142 (MQ=255) ccACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGCcg > 1:924936/1‑143 (MQ=255) gCGTGCGAGGCGCTCTTTTTCTTCGGGCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCCGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGaa < 2:265178/143‑1 (MQ=255) gCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGCAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCCACAGCTCATTGCGGTCGCCGCGaa > 2:256309/1‑143 (MQ=255) gggCGGTGCGCTCTTTTGCTTCGGGCTGGGTGGCCTGGCGGTCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa < 2:75009/143‑1 (MQ=255) gcTCTTTTTCTTCGGTCTGAGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCGTTGAGGTAGCGGCGAATAGACttctgg > 2:133552/1‑138 (MQ=255) tttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TACCCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCACCTCGTTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCg > 2:382469/1‑143 (MQ=255) ttgcttgGGCCTGGGGGGTCTGGCGCTCGTGCGACGGCGTGT‑TACCCGAACTGGAAGGCAGCGCCGCGCCGCCATGATGGGG‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAct < 2:5225/140‑2 (MQ=255) cttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTTT‑TAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgag < 1:898791/143‑1 (MQ=255) tcGGTCTGGGTGGCCTTGCGGTCGGGCCACGGCGTGT‑TGACCGAACTGGAAGGCAGCGCCGCACCGCCAGGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaa < 2:33024/143‑1 (MQ=255) ggcTGGGTGGTCTTGGCAGCGTGCCACGGCGTGG‑TGACCGGACTGGGAGGCCGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATg < 2:735872/141‑1 (MQ=255) tGGGTGGCGGTGCGAGCGTGCCACGGCGTGT‑TAACCGTACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctc < 2:33968/143‑1 (MQ=255) tgggCCTTGCGATCGTGCCACGGCGTGT‑TAACCGGCCTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAc < 2:861878/141‑1 (MQ=255) tGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCGGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc > 1:702213/1‑143 (MQ=255) ttGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCCCATTGAGGTCGCGGCGAATAATCTGCGGGCTCACAGGGAAATGCTCCACCAGgtc > 2:478072/1‑143 (MQ=255) gggATCGTGTCACGGTTTTT‑TAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAC‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt < 1:416962/141‑1 (MQ=255) gCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGc > 1:361871/1‑130 (MQ=255) gCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGc < 2:361871/130‑1 (MQ=255) gATCGTGCCACGGC‑TGTGTAACCGAGCTGGATGGCATGGCCGTACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCAAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg < 2:770619/143‑1 (MQ=255) gATCGGGCTACGGCGTGT‑TCACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGCATCAGGTTGTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCACTTCg < 2:793796/143‑1 (MQ=255) aTCGTGCCACGGCGTGT‑GAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCgg < 1:362744/143‑1 (MQ=255) tAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTCCGGTACTGACATAACCctgc > 1:786455/1‑143 (MQ=255) gcGAAGTGGAAGGCAGCGCCGCACCGCGATGAT‑GGCGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGGCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGt < 1:341573/142‑1 (MQ=255) cggAACTGGAGGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGt < 1:798913/141‑1 (MQ=255) ccGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTGTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGt < 2:435112/143‑1 (MQ=255) cGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCCGCTCAGTGAGGTCGCGGCGAATAGTCTGCGGGCCGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACACAGCCGtt > 2:624341/1‑143 (MQ=255) cTGGGAGGCAGCGCCGCACCGTCTTGTT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGACATGCTTTATCTGCTTTTCGGTACTGACATAACCCTGCTGTTTAAc < 2:527750/143‑1 (MQ=255) cTGGAGGGCAGCGCCGCACCGCCAGGAT‑GGCGCGGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc < 2:356089/143‑1 (MQ=255) cTGGAAGGCAGCGCCGCACCGCTATGAT‑GGCGCAGGATTAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc < 1:285972/143‑1 (MQ=255) tGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGACTAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCCCTTCGGTACTGACATAACCCTGCTGTTTAccc > 1:650893/1‑143 (MQ=255) tGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTacac > 1:603205/1‑140 (MQ=255) aGGCAGCGCCGCACCGCCATGCT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt < 1:830274/143‑1 (MQ=255) cgcACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTGCTGACATAACCCTGCTGTTTAACCAGTTCGATAATAc > 2:55119/1‑143 (MQ=255) cGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGACATGCTCGACCAGCGCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtg > 1:441212/1‑143 (MQ=255) cGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtg < 2:702213/143‑1 (MQ=255) tgat‑gGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGGCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACg > 1:466101/1‑143 (MQ=255) | GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACG > NC_000913/3560316‑3560590 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |