New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 232553 =55 (1.060)6 (0.130) 6/258 5.8 10.0% intergenic (+4/+44) yafE/mltD putative S‑adenosyl‑L‑methionine‑dependent methyltransferase/putative membrane‑bound lytic murein transglycosylase D
?NC_000913 232584 = 58 (1.220)intergenic (+35/+13) yafE/mltD putative S‑adenosyl‑L‑methionine‑dependent methyltransferase/putative membrane‑bound lytic murein transglycosylase D
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CGCCAGAAGCGTTAGTAGACGCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGAT  >  NC_000913/232413‑232695
                                                                                                                                             |                                                                                                                                              
cGCAAGAAGAGTTAGTAGACGCTATTCACAGTTACCAACAGAAATCTAAAACATAAATGAAAACATATATTGCAATGCAGAATGATGGCTTATTAACAAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATaaaa                                                                                                                                              <  2:625235/143‑1 (MQ=255)
    aGAAGCGTTAGTAGACGCTATTCGCATTTACCAACAGAGAGCATCGACCGCGGTGCGAACGTATCTTGCCTTGCAGAATGATGGCTTTTTCAACAGTGATATCATCAGGCTAGATGCACATAAAGCGGCATAAATAAAAAAgg                                                                                                                                          <  2:385436/143‑1 (MQ=255)
        gCGTTAGTAGACGCTATTCGCATTTACCAAGAGAACTCATCGTCAGTGCTGAAAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCAcc                                                                                                                                      <  2:625464/143‑1 (MQ=255)
        gCGTTAGTAGACGCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATCGAGCGGCATCAATAAAAGAGGCAcc                                                                                                                                      >  1:920573/1‑143 (MQ=255)
         cGTTAGTAGACGCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGCTGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATCAATAAAAAAGGCACCg                                                                                                                                     >  2:164455/1‑143 (MQ=255)
            tagtagACGCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGGCCCgggg                                                                                                                                  >  1:160363/1‑143 (MQ=255)
              gtagACGCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCCCATAAAGCGGCATAAATAAAAAAGGCACCGGGGGa                                                                                                                                <  1:859537/143‑1 (MQ=255)
              gtagACGCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGa                                                                                                                                >  1:238330/1‑143 (MQ=255)
                  aCGCTATTCGTATTTACCAACAGAGCGCATCGATAGAGGTGATAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCg                                                                                                                            <  1:379235/143‑1 (MQ=255)
                              tttACCCACAGATCCCATCGACAGAGGTGAGAACGTATCTTGCCTTGCAGCATGATGGCTTTTTCACCAGTGCTCTCATCATGGTAGATGCACATAAAGCGGCATAACTAAAAAAGGCACCGGGGGAATCGGTGCCTTTttat                                                                                                                <  1:139541/143‑1 (MQ=255)
                                   cAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATCAATAAAAAAGGCACCGGGGGAATAGGTGGCTTGTTATTATCt                                                                                                           >  2:139270/1‑143 (MQ=255)
                                   cAACAGAGCGCATCGACAGAGGCGAGAACCAATCGCCCCTAGCGTTCTGATGGCAGTTTCACCAGTCATATCAGCATGGTAGAGGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTCTCATTATCt                                                                                                           >  2:634617/1‑143 (MQ=255)
                                    aaCAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTGTTATTctctc                                                                                                          >  2:304211/1‑142 (MQ=255)
                                      tagagCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGt                                                                                                        <  2:353512/142‑1 (MQ=255)
                                       agagCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTtattatgcggct                                                                                                       >  2:623491/1‑137 (MQ=255)
                                        gagCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACAGGGGGAATCGGTGCCTTTTTATTATCTGGttt                                                                                                      >  2:894127/1‑143 (MQ=255)
                                        gagCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTGCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGttt                                                                                                      <  1:35525/143‑1 (MQ=255)
                                             acccGACATAGTGGAGAACTCAGTTTGCCTGGCAGAATGTGGGCGCTCTGACCAGTGATATCATCATGGTAGATGCACATAAATCGGCATGAATAAAAAAGGCACCGGGGGAAACGGTGCCTTTTTATTATCTGGTTTGTCAg                                                                                                 <  2:404857/140‑1 (MQ=255)
                                              aTCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTAACTGGTTACTCAgg                                                                                                >  2:661754/1‑143 (MQ=255)
                                                 gACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGGTTGTCAGGAAt                                                                                             >  2:797632/1‑143 (MQ=255)
                                                        ttGAGAACGTATTTTGCCTGGTAGAATTATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAt                                                                                      <  2:790608/142‑1 (MQ=255)
                                                          gagaACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAtgt                                                                                    >  2:525479/1‑143 (MQ=255)
                                                              aCGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACCTAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATGATCTGGTTTGTCAAGAATATGGCAtgttgtt                                                                                >  1:896985/1‑143 (MQ=255)
                                                              aCGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATATTGCGGCATATATAAAAACGGCACCGGGGGAATCGGAACCTTTTTATAATCTGGTATGTCAGGAATCTGGCAtgttgtt                                                                                >  1:28217/1‑143 (MQ=255)
                                                                gTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAtgttgttgt                                                                              >  1:588649/1‑143 (MQ=255)
                                                                gTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATAGGATTTGTCAGGAATCTGGCAtgttattga                                                                              >  1:818642/1‑142 (MQ=255)
                                                                  aTTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGttt                                                                            >  1:858296/1‑143 (MQ=255)
                                                                   ttttGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGTCATAAATAAAATAGGCTCCTGGTTAATCGGTGCCTTTTTATTATTTGGTTTGTTAGAAATCTGACATGTTGTTGtttt                                                                           >  1:423516/1‑143 (MQ=255)
                                                                   ttttGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGCATCGGTGCCTTGTCATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGtttt                                                                           >  1:257600/1‑143 (MQ=255)
                                                                      tGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTca                                                                        <  1:57144/143‑1 (MQ=255)
                                                                         cTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCTGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGGTGTTTTTCacaa                                                                     >  1:577167/1‑143 (MQ=255)
                                                                                aagtatgGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGt                                                              <  2:331644/139‑1 (MQ=255)
                                                                                    atgGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGCAATCTGGCATGTTGTTGGTTTTCACAAACAACGTCAGCt                                                         >  1:129824/1‑143 (MQ=255)
                                                                                       gCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCCTAAATAAAAAAGGCACCGGGAGAATCAGAGCGTTGTGTTGGTATGGTTTTTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAAAGTCAGCTTa                                                       >  2:236355/1‑143 (MQ=255)
                                                                                       gCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTa                                                       >  2:85758/1‑143 (MQ=255)
                                                                                          ttttCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCg                                                    >  2:794822/1‑143 (MQ=255)
                                                                                           gtccACCAGTGATATCATCACGGTAGACGCACATAAAGCGGCATAAATAAAAAAGGCCCCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGc                                                   <  2:675178/140‑1 (MQ=255)
                                                                                              cagcAGTTATATCATCATGGTTGTTGCATATAATGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGTAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTg                                                <  2:316867/140‑1 (MQ=255)
                                                                                               aCCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCCCAAACAACGCCAGCTTATCGCCTgg                                               >  1:281156/1‑143 (MQ=255)
                                                                                                ccAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGt                                              <  2:758174/143‑1 (MQ=255)
                                                                                                 cAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAACTAAACATGGAACCCGGGTGGGCGCACCACTCCTGGGTTTGGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTGTCGGCGGGtt                                             >  1:700941/1‑143 (MQ=255)
                                                                                                    tGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGCTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCa                                          <  1:618597/143‑1 (MQ=255)
                                                                                                       tatCATCATGGTAGATGCCCATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCGTGGTTGCAGAt                                       >  1:640994/1‑143 (MQ=255)
                                                                                                         tcaccaTGGTAGATGCACATAAAGCGGCATAAATTAAAAATGCACCGGGGGAATCGTTGCCTTTTTATTATCTGGTTTGTCAGCAATCTGGCATGTTGTTGTTTTTCACAAACAATGTCAGCTTATCGCCTGGTTGCAGATTc                                     <  2:67765/143‑1 (MQ=255)
                                                                                                           atcatGGTAGATGCACATAAAGCGGCATAAATAACAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGc                                   <  2:161363/143‑1 (MQ=255)
                                                                                                           atcatGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGACAGCTTATCGCCTGGTTGCAGATTCGc                                   <  2:117192/143‑1 (MQ=255)
                                                                                                            tcatGGTAGGTGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCa                                  <  2:437274/143‑1 (MQ=255)
                                                                                                               tGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAgtg                               >  1:5627/1‑143 (MQ=255)
                                                                                                                         acaTAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCTTCTGGTTGCAGAGTCACACTGCCGCGGTTcc                     >  2:672019/1‑143 (MQ=255)
                                                                                                                           aTAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTAGTTGCAGATTCGCAGTGTCGCTGTTCCAg                   >  2:119481/1‑143 (MQ=255)
                                                                                                                            ttaaGCGGCATTAAAAAAAAATGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAg                   <  2:160363/141‑1 (MQ=255)
                                                                                                                               aGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCTGATTCGCGGTGTCGCTGTTCCAGCGCa               >  1:483726/1‑143 (MQ=255)
                                                                                                                                   gCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCCGCGCATcac           >  1:266146/1‑143 (MQ=255)
                                                                                                                                     ataaataaAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCACCTCAt         >  2:657507/1‑143 (MQ=255)
                                                                                                                                       taaataaaAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCCGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATc        >  1:216648/1‑143 (MQ=255)
                                                                                                                                             aaaaaGGCACCGGGGGTATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCTCTGTTCCAGCGCATCACATCTTTGAt  <  2:281156/143‑1 (MQ=255)
                                                                                                                                             aaaaaGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGAt  >  1:746883/1‑143 (MQ=255)
                                                                                                                                             |                                                                                                                                              
CGCCAGAAGCGTTAGTAGACGCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGAT  >  NC_000913/232413‑232695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.