New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4454798	60 (1.160)	5 (0.100)	5/270	6.6	8.2%	coding (812/999 nt)	fbp	fructose‑1,6‑bisphosphatase I
	?	NC_000913	= 4454801	54 (1.090)					coding (809/999 nt)	fbp	fructose‑1,6‑bisphosphatase I
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGC‑CATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCTTCTTCCTGG  >  NC_000913/4454660‑4454938                                                                                                                                               |                                                                                                                                          tggtggTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGC‑CATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAg                                                                                                                                          <  1:484919/143‑1 (MQ=255)     cGTTGCCGACAAAGAATGAGCGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGC‑CATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTg                                                                                                                                      <  1:872200/143‑1 (MQ=255)      gTTGCCGACAAAGACTGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGC‑CATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCATTTTGc                                                                                                                                     <  1:316609/143‑1 (MQ=255)      gTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGC‑CATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGc                                                                                                                                     <  1:843577/143‑1 (MQ=255)            gACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGC‑CATCGCTCGCTTTCCCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAGACGCGGTTTGCCGTCCg                                                                                                                               >  1:796434/1‑143 (MQ=255)             aCAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGC‑CATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCCGTTTGCCGCCCgg                                                                                                                              >  2:942422/1‑143 (MQ=255)               aaaGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGC‑CATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGt                                                                                                                            <  1:63417/143‑1 (MQ=255)                     tGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGC‑CATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGGATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGCCCGGGtggctgg                                                                                                                      >  1:71854/1‑143 (MQ=255)                     tGAACGGCGCTGGTACAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGC‑CATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGtggctgg                                                                                                                      <  1:12978/143‑1 (MQ=255)                              cTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGC‑CATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTg                                                                                                             <  2:588501/143‑1 (MQ=255)                                  tGCAGGGTTTCCGGGATGAGCTCGAGAAGACGTTGTCTGCGCGTCG‑TCTCGGTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTa                                                                                                         <  1:944597/143‑1 (MQ=255)                                   gCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGC‑CATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTag                                                                                                        >  2:841626/1‑143 (MQ=255)                                   gCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGC‑CATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTag                                                                                                        <  2:850858/143‑1 (MQ=255)                                    cAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGC‑CATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTaga                                                                                                       >  2:710215/1‑143 (MQ=255)                                     cgggTTTCCGGGATGATATCCAGAATACGCTCTTTGC‑CATCGCTAGGTTTATCCCCTGTGTGTTGCCCCAGAAATGCCAGCGGGTTGCACTCATACAGCAAAGGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTagag                                                                                                      <  2:201532/142‑1 (MQ=255)                                             cGGGATGATATCCAGAATACGCTCTTTGC‑CATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCGTGGGTAGAGAGAGATag                                                                                              >  1:317862/1‑142 (MQ=255)                                                ggtgatATCCAGAATACGCTCTTTGC‑CATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCACCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGc                                                                                           <  2:776638/141‑1 (MQ=255)                                                         cAGAATACGCTCTTTGC‑CATCCCTCGCGTTACCGCCCGCTTGTTCCGCCAGTAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTcagca                                                                                  <  2:440113/143‑1 (MQ=255)                                                          aGAATACGCTCTTTGC‑CATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCCTGGGCAGAGATAAATACCGCCTTGcagcgg                                                                                 >  2:933495/1‑141 (MQ=255)                                                          aGAATACGCTCTTTGC‑CAGCTCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTcagcag                                                                                 <  1:831164/143‑1 (MQ=255)                                                           gAATACGCTCTTTGC‑CATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAgg                                                                                <  1:827789/143‑1 (MQ=255)                                                                   tctTTGC‑CATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTg                                                                        >  1:98042/1‑143 (MQ=255)                                                                           cATCGCTCGCTTTATCGCCCGCTTGTTCCGCCAGGAATGCCAGCGGGATGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATcc                                                                 <  1:299605/143‑1 (MQ=255)                                                                            aTCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCcg                                                                >  1:683462/1‑143 (MQ=255)                                                                             ccgcgcgctTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCcgc                                                               <  1:702976/142‑1 (MQ=255)                                                                                  cgctCTACCGCCCGCTTGCTCCGCCAGGAATGCCATCGGGTTGCACTCATCCAGCAAACGCACTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCa                                                          <  1:482286/143‑1 (MQ=255)                                                                                    ctTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGACACCAACTTCGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGt                                                        >  1:698245/1‑143 (MQ=255)                                                                                     tttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTggg                                                                                                           <  2:915410/91‑1 (MQ=255)                                                                                     tttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTggg                                                                                                           >  1:915410/1‑91 (MQ=255)                                                                                      ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGa                                                      <  2:728560/143‑1 (MQ=255)                                                                                        aCCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAAc                                                    <  2:493005/143‑1 (MQ=255)                                                                                             ccgcTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAGTCCGCGACCAGCGCACCGata                                               >  2:848589/1‑143 (MQ=255)                                                                                                gttGTTCCGCCAGGAATGCCATCGGGTTGCGCGCATACAGCAAACGCAGTTTGCCGGCCGGGTGGCCGGCGGTGCTTGGGTAGAGATAAATACCGCCTGTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATaa                                            <  2:125374/142‑1 (MQ=255)                                                                                                    ttCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCAGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTg                                        >  1:716246/1‑143 (MQ=255)                                                                                                       cGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAGTCCGCGACGCGTGAACCGGTATAACGTGTgg                                     >  1:491290/1‑143 (MQ=255)                                                                                                       cGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGCACCGAGATAACGTGAgg                                     >  2:691055/1‑143 (MQ=255)                                                                                                       cGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGTTATGACGTGCgg                                     >  2:687650/1‑143 (MQ=255)                                                                                                        gCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTTCGGTTGCAATCCGCGACCAGTGAACCGATATACCGTGAGGt                                    >  1:449225/1‑143 (MQ=255)                                                                                                         ccAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGta                                   >  1:551590/1‑143 (MQ=255)                                                                                                            ggAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGGTACGGTGGAAATCCGCGGCCAGTGAACCGATATGACGTGCGGtagca                                >  1:303001/1‑140 (MQ=255)                                                                                                              actGCCATCGGTGTGCGCTCATACAGCAAACGCAGGTTGCCGGCCGGGTGGCTGGCGGTGCGTGGGTAGAGATAACTACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAgg                              <  2:266434/141‑1 (MQ=255)                                                                                                                tGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGGGAGGTGTAAGAGc                            >  1:23025/1‑143 (MQ=255)                                                                                                                   cATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTAGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGAATAAggacagt                         >  2:410367/1‑138 (MQ=255)                                                                                                                    aTCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAGCGTGAGGTATAAGGGCGGtt                        >  1:70764/1‑143 (MQ=255)                                                                                                                     tCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTAGGGTAGAGATAAATACCGCCTTACAGCAGGTTACGGTGGAAATCCGCGACCAGTGATCCGATAGAACGTGAGGTATAAGGGCGGGtg                       >  2:768370/1‑143 (MQ=255)                                                                                                                       gggTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTtggt                     <  1:710215/143‑1 (MQ=255)                                                                                                                        tgTTTCACTAATATAGGCAACGCAGTGTGCCGGCGGGGGGGCGGGCGGTGCGTGGGTAGAGAGAAATACCGGCTTGCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTtggtg                    <  1:269273/142‑1 (MQ=255)                                                                                                                        tgTTGCACTCATACACCAACCGCAGTTTGCCGGCCGGGTGGCTGGCGGTGCTTGGGTAGAGATACATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTtggtg                    <  1:232128/142‑1 (MQ=255)                                                                                                                        gtctGCACTCATGCAGCCAACGCAGTGTGCCGTGCGGGTGGCTGGCGGTGCTTGGGTAGAGGTAAATACCGCCGTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTtggtg                    <  2:734358/140‑1 (MQ=255)                                                                                                                          ttGCAGGCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGa                  <  1:942422/143‑1 (MQ=255)                                                                                                                               cTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTAt             <  1:802511/143‑1 (MQ=255)                                                                                                                                 caaaCAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTAtct           <  1:841626/140‑1 (MQ=255)                                                                                                                                     cAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATcttctt       >  1:210889/1‑143 (MQ=255)                                                                                                                                     cAGCAAACGCAGTTTGCCGAACGCATGGGGGTCGTTGCTTGGGTAGCTATGAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATcttctt       <  1:130445/143‑1 (MQ=255)                                                                                                                                      aGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCttcttc      >  1:366059/1‑143 (MQ=255)                                                                                                                                       gCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATGCGCGACCAGTGAACCGATATAACGTGAGGTATCAGGGCGGTTGGTGGATTTATCTTCTTcc     >  2:612771/1‑143 (MQ=255)                                                                                                                                        cAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATCCCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCTTCTTCCt    <  1:669703/143‑1 (MQ=255)                                                                                                                                          aaCGCAGTTTGCCGTCCGGGTGGCTGGCGGTGATTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATCAGGGCGGTTGGTGGATTTATCTTCTTCCTgg  >  2:500722/1‑143 (MQ=255)                                                                                                                                               |                                                                                                                                          TGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGC‑CATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCTTCTTCCTGG  >  NC_000913/4454660‑4454938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.