| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,173,361 | 0 | C | . | 96.4% | 114.4 / ‑2.7 | 28 | pseudogene (2/442 nt) | gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (15/12); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
| * | NC_000913 | 2,173,362 | 0 | C | . | 96.4% | 113.7 / ‑3.1 | 28 | pseudogene (1/442 nt) | gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (15/12); minor base G (0/1); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
ATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGT > NC_000913/2173221‑2173496 || tctgccACGATGCACCGCGGCCGCCATCGCTACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATaccaccg < 2:62776/139‑2 (MQ=255) cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTGGCCGATaccaccgccgta > 1:256954/1‑137 (MQ=255) cACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCca > 1:123474/1‑143 (MQ=255) tcggagaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATTGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAg < 2:271509/139‑1 (MQ=255) gCCGATGGGGGCAAGATCGCCAAACGGCAGCAACTGATTACCCGGCACACAAACAGCAATAAAAAAGAT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCCGCAGCAACGGCGGATCACGGCCagaca > 2:180044/1‑139 (MQ=255) cGATGGTGGCAAGATCGCCAAACGGCAGCAACTGATTACCCGGCACACAAACAGCAATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATAAgg > 2:264180/1‑143 (MQ=255) gATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGG‑GAGTGGGATAAAAATCAGGCTTGCCGAGACCA‑‑CCGCCGTATGTCCCAGCAGCAAAGCCGGATCAAGGCCAAACAGGa > 1:359220/1‑143 (MQ=255) aTCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGCTCAAGGCCAATCAGGAACTCCTGAccgc > 1:623464/1‑143 (MQ=255) tCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgcc < 1:351839/143‑1 (MQ=255) cAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAAc < 2:28989/143‑1 (MQ=255) acaCGGCAGCCCCTGATTACCCGGCACACAAACAGCAATGAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACt < 2:5691/141‑1 (MQ=255) aaaCGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGT‑GAGTGGGATAAAAATCAGGCGCGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGCCCGCCGGACt > 1:504800/1‑143 (MQ=255) aaaCGGCAGCACCTGACTACCCGGCACACAAACAGCAATTAACATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACt < 1:583759/143‑1 (MQ=255) ggCAGCACCTGATTCCCCGGCACACAAACAGCAAAT‑AGATGGTGGAGGTGGATAAAAATCAGGCTTGCCGATAC‑‑CGCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGc < 2:165805/143‑1 (MQ=255) ggCAGCACCTGATTACCCGGCACACAAACAGCAATCGTAATTGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGc < 2:165839/143‑1 (MQ=255) gcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGt < 2:184022/143‑1 (MQ=255) cTGATTACCCGGCACACAAACAGCAATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGCGCCAATGCCAATCAGGAACTCCTGACCGCCGGACTTCACCTGTaaaa > 1:335510/1‑142 (MQ=255) gtACCCGGCACACAAACAGCAATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACt < 1:777599/142‑1 (MQ=255) ccGGCACACAAACAGCAATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACCCCTGACCCCCGAACTTCGCATGTAAACCCCTACGa > 2:503641/1‑143 (MQ=255) ccGGCACACAAACAGCAATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTACCAGCAGCAACGCCGGATCAAGGCCTATCAGGACCTCCTGACCGCCGAACTTGCCCGGTAAACGACAACGa > 2:584761/1‑143 (MQ=255) ccGGCACACAAACAGCAATTAAAAAGGA‑GAGTGGGATAAAAATCAGGCTTGCCGATACAA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGa > 1:473586/1‑143 (MQ=255) caAACAGCAATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCACAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGACCTGCACCCGTAAACGACTACCAGcccggtg > 2:591953/1‑138 (MQ=255) aaaCAGCAATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTa > 1:135228/1‑143 (MQ=255) aaCAGCAATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAg < 2:256954/143‑1 (MQ=255) aaaTGGT‑GAGGGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGCCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGtt < 2:621927/143‑1 (MQ=255) attGGGAGAAAGTTCAGTCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCACAACCGCCGGAGCAAGGCCAATCAGGAACGCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatc < 2:92395/141‑1 (MQ=255) gggATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTGCGCCTGTAAACGACTACGAGCCTGCTTCGCGATGGGCGTTAAAccatccat > 2:255164/1‑143 (MQ=255) aTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGGTAAACCCCCCATGATGGGttt > 2:143615/1‑143 (MQ=255) aGGCGTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCAGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACGTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAAt < 2:69123/143‑1 (MQ=255) ccGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCCTAGCGATGGGCGTTAAACCATCCATGATAGGCTTAATCACCCGt > 2:500718/1‑143 (MQ=255) || ATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGT > NC_000913/2173221‑2173496 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |