New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1826807	45 (1.010)	4 (0.090)	4/272	6.0	8.2%	coding (117/969 nt)	astE	succinylglutamate desuccinylase
	?	NC_000913	= 1826851	46 (1.070)					coding (73/969 nt)	astE	succinylglutamate desuccinylase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGT  >  NC_000913/1826718‑1826991                                                                                                                                       |                                                                                                                                             gcgcCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAGCGCCAtt                                                                                                                                     >  1:329072/1‑143 (MQ=255)  cgcCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCCTCGCCCAGCCAGCGCCAGCGAACGCCGTTg                                                                                                                                    >  1:464321/1‑143 (MQ=255)    ccAAGCAGCGCGTCCAGCAGCTCCAGGTGTGCCGTCTCATTAGGGTGTAGTGGCTC‑GCAAGCAGCCGCCTGGTTTGGCGGTGGAGTTAATGGCCTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGAt                                                                                                                                  <  2:783277/143‑1 (MQ=255)     cAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGCTGAtt                                                                                                                                 >  2:451575/1‑143 (MQ=255)     cAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAATGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCCCACCATCGCCCAGCCAGCGCCAGCGAACGCCGCTGAtc                                                                                                                                 >  2:206705/1‑142 (MQ=255)      aaGCAACGCGTCCAGCATCTCCACAGGTCCCGTCTCATTACCGTGTATTCCCGCTGAAATCACGAGTGCCCCTT‑GCGCTGGCGCCCATGGCGTCAGGTCCAGCACACCGTCGCCCCCCCGGCGCCCGCGACCCCCGCTgctag                                                                                                                                >  2:719028/1‑139 (MQ=255)         cAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCCGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGGGCCAGCGGACGCCGTTGACTtctc                                                                                                                             >  2:801708/1‑143 (MQ=255)              cgTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTctcgctcg                                                                                                                        <  2:540989/143‑1 (MQ=255)                  cAGCACCTCGACAGGTGCCGTCTCATTACCGTGTATGCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGa                                                                                                                    <  2:367504/143‑1 (MQ=255)                               ggTGCCGTCCCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTAAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGtttttt                                                                                                       <  1:421511/143‑1 (MQ=255)                                gTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCGGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGttttttg                                                                                                      >  1:709713/1‑142 (MQ=255)                                gTGCCGGCTCATTACCGGGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTa                                                                                                      <  1:326066/143‑1 (MQ=255)                                   ccGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGGTCCAGCACACCATCGCCCAGCCAGCGCCAGGGAACGCCGTGGAGTGCACACTCGCGGATAACCGGTCTTGCAccc                                                                                                   >  2:234991/1‑143 (MQ=255)                                         cATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAg                                                                                             <  1:330610/143‑1 (MQ=255)                                          aTTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCCGCCAGCGCCAGCGAACGCCGCTGATTTCTCGCTCGGTGATAACCGGTTTTTTCCCCGTTACgg                                                                                            >  2:374761/1‑143 (MQ=255)                                                        ccGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCATCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGaaaa                                                                              >  1:506303/1‑143 (MQ=255)                                                                aTCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTGTACCCGTTCCGGTCAGAGCCAGAAACTTATCCAt                                                                      >  2:554115/1‑143 (MQ=255)                                                                  caccacTGCGCCTT‑GCGGTGGCGGTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTg                                                                    <  1:487903/143‑1 (MQ=255)                                                                   aCCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACGCGTGAAGGTCAGAGGAGGGAAAGTATCCATGGc                                                                   >  1:14267/1‑143 (MQ=255)                                                                     cAGTGCGCCTG‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGccc                                                                 <  2:636830/143‑1 (MQ=255)                                                                      aGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGcccc                                                                >  2:13892/1‑143 (MQ=255)                                                                           gcCTC‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCccctccc                                                           <  2:333479/143‑1 (MQ=255)                                                                               t‑GCGGTGGCGTTAATGGCGGCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTcgc                                                       <  1:308641/143‑1 (MQ=255)                                                                                     tGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGGACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTACGGTCAGAGCACGAAAATCATCGATTGCCCCCTCCCTCGCGCCgg                                                  >  2:175594/1‑143 (MQ=255)                                                                                        cggtAATGGCGGCAGTTCCAGCACACCATCGCGCAGCTATTTGTTTCGTACGCCGTTGATTGCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAAc                                               <  2:166063/140‑1 (MQ=255)                                                                                        cggtAATGGCGGCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTGCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAAc                                               <  1:147949/140‑1 (MQ=255)                                                                                         gTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACg                                              >  1:79878/1‑143 (MQ=255)                                                                                         gTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGAGCCAGCGAACGCCGTTGCTTTCTCGCCCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGACAACGAAAAGTAGCCATCGCCGCCTCCCTCCAGCTgtgacc                                              >  2:172959/1‑138 (MQ=255)                                                                                          ttAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACgg                                             >  1:478046/1‑143 (MQ=255)                                                                                           tAATGGCGTCAGTTCCAGCACACCATCGCGCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGa                                            <  2:452257/143‑1 (MQ=255)                                                                                              tGGCGTCAGTGCCAGCACACCATCGCCCAGGGAGCGCCAGCCAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATaa                                         <  1:333296/143‑1 (MQ=255)                                                                                                gCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCCGCGAACGCCGTTGATCTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTCAGGTCAGAGCAAGCAACTTATCCATTGCACCCCGCCTCGCTCTGGAACAGCTaacc                                       >  2:633757/1‑141 (MQ=255)                                                                                                gCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAaac                                       <  2:324693/143‑1 (MQ=255)                                                                                                gCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAaac                                       >  1:546378/1‑143 (MQ=255)                                                                                                gCGTCAGTTCCAGCACACCATCGCCCAGCAAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGGACGGATaagc                                       >  2:246861/1‑141 (MQ=255)                                                                                                    cAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAaaccgaa                                   <  1:285128/143‑1 (MQ=255)                                                                                                    cAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAaaccgaa                                   <  1:653666/143‑1 (MQ=255)                                                                                                        tCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGGTGATGTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAAaccgaaccga                               <  1:434546/143‑1 (MQ=255)                                                                                                          cAGCACACCACCGCCCGGCCGGCGGTAGCGACGGCCGTTGATGTCTCGCTCGGTGATAACCGGTTTTTTTCCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAAACCGAACCgaga                             <  2:191803/143‑1 (MQ=255)                                                                                                           aGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAAACCGAACCGAGAt                            >  1:797599/1‑143 (MQ=255)                                                                                                              acacCATCGCCCGGCCAGCGCGAGCGAGCGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAAACCGAACCGAGATTCa                         <  2:340518/143‑1 (MQ=255)                                                                                                              acacCATCGCCCAGTCAGCGCCAGTGAACGCCGTTTATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAAACCGAACCGAGATTCa                         <  1:280686/143‑1 (MQ=255)                                                                                                                      gCCCAGCGAGCGCCAGCGAACTCCGTTGATTTCTCGCCCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTgg                 <  2:751542/143‑1 (MQ=255)                                                                                                                        ccagccagCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCt               <  2:464321/143‑1 (MQ=255)                                                                                                                         cggccagcgcccgcGTTCGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTGCCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTc              <  2:430050/141‑1 (MQ=255)                                                                                                                                   cagcgAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTACTTGGCTCAATAcgtgca    >  2:1968/1‑138 (MQ=255)                                                                                                                                     gcgAACGCCGTTGATTTCTCGCTCGGTGATAACGTTCGGTTTGCCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGt  <  1:332667/143‑1 (MQ=255)                                                                                                                                       |                                                                                                                                             GCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGT  >  NC_000913/1826718‑1826991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.