Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 3,527,658 Δ5 bp coding (1190‑1194/2136 nt) yrfF → putative RcsCDB‑response attenuator, inner membrane protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3527658 3527662 5 72 [0] [0] 72 yrfF putative RcsCDB‑response attenuator, inner membrane protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 35276570 (0.000)72 (1.190) 65/284 0.0 100% coding (1189/2136 nt) yrfF putative RcsCDB‑response attenuator, inner membrane protein
?NC_000913 3527663 = 0 (0.000)coding (1195/2136 nt) yrfF putative RcsCDB‑response attenuator, inner membrane protein

GTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACA  >  NC_000913/3527521‑3527804
                                                                                                                                              |                                                                                                                                             
gtgctgtttatgctgttattctggatcccgctggatatgccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgca                                                                                                                                               >  1:825634‑M2/143‑143 (MQ=255)
  gctgtttatgctgttattctggatcccgctggatatgccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGc                                                                                                                                             >  2:1078011‑M2/141‑143 (MQ=255)
   ctgtttatgctgttattctggatcccgctggatatgccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCg                                                                                                                                            >  2:540716‑M2/140‑143 (MQ=255)
     gtttatgctgttattctggatcccgctggatatgccgctgaaattcactcactcatggatgaaaggcgcgcagacccttgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGt                                                                                                                                          >  2:1049643‑M2/138‑143 (MQ=255)
     gtttatgctgttattatggatcccgatggatatgccgttgaaattcactctctcatggatgaaaggcgcgcagactattgaagccaccagcgtaaaacaactggctgacgatggcgtgagggtaggagatacattgcAGCGGt                                                                                                                                          >  2:847106‑M2/138‑143 (MQ=255)
     gtctatgctgttattctggctcccgctggatatgccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGt                                                                                                                                          <  1:160893‑M2/6‑1 (MQ=255)
        tatgctgttattctggatcccgctggatatgccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACg                                                                                                                                       <  1:545684‑M2/9‑1 (MQ=255)
         atgctgttattctggatcccgctggatatgccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACgg                                                                                                                                      >  2:788885‑M2/134‑143 (MQ=255)
           gctgttattctggatcccgctggatatgccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGa                                                                                                                                    >  1:66306‑M2/132‑143 (MQ=255)
            ctgttattccggaccccgctgcagacgccgcttaacttcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGaa                                                                                                                                   <  2:1097357‑M2/13‑1 (MQ=255)
             tgttattctggatcccgctggatatgccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAAt                                                                                                                                  >  1:436988‑M2/130‑143 (MQ=255)
              gttattctggatcccgctggatatgccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAAtg                                                                                                                                 >  1:1076486‑M2/129‑143 (MQ=255)
               ttattctggatcccgctggatatgccgctgaaattcactctctcatggatgaaaggcgcgccgaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGTTACGGGAAtgt                                                                                                                                >  1:873007‑M2/128‑143 (MQ=255)
                 attctggatcccgctggatatgccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacggtggcgtgcgggtaggcgacacattgcAGGGGTACGAGACCgtgt                                                                                                                              >  1:450165‑M2/126‑143 (MQ=255)
                  ttctggatcgcgctggatatgccgctgaaattcactctctcatggatgcaaggcgcgcagaccattgcagccaccagcgtaaaagaactggctgacgctggcgggcggggaggcgagacattacAGCGGTACGggaatgggaa                                                                                                                             >  2:712832‑M2/125‑139 (MQ=255)
                  ttctggatcccgctggatatgccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTa                                                                                                                             <  1:569759‑M2/19‑1 (MQ=255)
                  ttctggatcccgctggatatgccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaacgggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTa                                                                                                                             <  2:850293‑M2/19‑1 (MQ=255)
                      ggatcccgctggatatgccgctgaaattcactctctcatggatgaaaggcgcgcagacaattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtcggcgatacattgcAGCGGTACGGGAAAGAGTaatag                                                                                                                         >  2:1009987‑M2/121‑142 (MQ=255)
                       gatcccgctggatatgccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgctacattgcAGCGGTACGGGAATGTGtaatagt                                                                                                                        >  1:90722‑M2/120‑141 (MQ=255)
                        atcgcgctggatatgccgctgaaattcgctctctcatgtattaaaggcgcgcagaccatagaagccaccagcgtaaaacaactggctgacgctggcgttcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTc                                                                                                                       <  2:1094819‑M2/25‑1 (MQ=255)
                        atcccgctgtatatgccgctgaaagtcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgtgtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTc                                                                                                                       <  1:674386‑M2/25‑1 (MQ=255)
                        atcccgctggatatgccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAGTGTGTAATATTc                                                                                                                       >  1:996699‑M2/119‑143 (MQ=255)
                               tggatatgccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacactgcAGCGGTAAGGGAATGCGTAATATTCGAACTTc                                                                                                                >  2:299458‑M2/112‑143 (MQ=255)
                                ggatgtgccgctgagattctctctctcatcgatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctagcgtgcgggtaggcgatacattgcAGCGGTACGGGAATTTGTAATATTCGAACTTcc                                                                                                               <  2:400833‑M2/33‑1 (MQ=255)
                                  atatgccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCgg                                                                                                             <  2:66306‑M2/35‑1 (MQ=255)
                                     tgccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagcccccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATCTTCGAACTTCCggacc                                                                                                          >  2:42762‑M2/106‑141 (MQ=255)
                                      gccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgatggcgttggggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACGTCCGGAAcc                                                                                                         >  2:269677‑M2/105‑143 (MQ=255)
                                       ccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccacccgcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGAGAATGTCTGATGTTCGAACCACCGGAaccc                                                                                                        >  2:120429‑M2/104‑142 (MQ=255)
                                       ccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGCGAATGCGTAATACTCGAACTTCCCGAaccg                                                                                                        >  2:532794‑M2/104‑142 (MQ=255)
                                        cgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTg                                                                                                       <  2:1044643‑M2/41‑1 (MQ=255)
                                         gctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggaaggcgatacattgcAGCGGTACGGCTATGTGTACTATTCGAACTTCCGGACCCTgg                                                                                                      >  2:524787‑M2/102‑143 (MQ=255)
                                          cggaaattcactctctcatggatgaaaggcgcgcggcccattgaggccaccagcgtaaaacaactggctggctctgtcgtccgggcaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGa                                                                                                     <  2:954777‑M2/43‑1 (MQ=255)
                                            gaaattcgctctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAgc                                                                                                   <  1:642419‑M2/45‑1 (MQ=255)
                                                    ctctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACGTCCGGAACCTGGAGCGCAAAAAc                                                                                           >  2:681429‑M2/91‑143 (MQ=255)
                                                    ctctctcagcgacgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAAc                                                                                           <  2:911987‑M2/53‑1 (MQ=255)
                                                     tctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAACGTGTAATATTCGAACTTCCGGGACCTGGAGCGCGAAAAcc                                                                                          >  2:1034825‑M2/90‑143 (MQ=255)
                                                         tcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGCACCTGGAGCGCGAGCACCAAtt                                                                                      >  2:24668‑M2/86‑143 (MQ=255)
                                                           atggatgaaaggcgcgcagaccattgaagccactagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCa                                                                                    <  1:555685‑M2/60‑1 (MQ=255)
                                                            tggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATAGTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCAc                                                                                   >  1:948653‑M2/83‑143 (MQ=255)
                                                              gatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacatcgcAGCGGTACGGGAATGTGTAATATTCGAACTTACGCAACCTGGAGAGCAAATACTAATTCACCt                                                                                 >  2:650122‑M2/81‑143 (MQ=255)
                                                               atgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCtt                                                                                >  2:946791‑M2/80‑143 (MQ=255)
                                                                  acacgcgcgcagaccattgaagccaccagcttaacacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCttttt                                                                             <  2:41336‑M2/67‑1 (MQ=255)
                                                                      gcgcgcggaccattgaagccaccagcggaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGCGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTAcc                                                                         <  1:155770‑M2/71‑1 (MQ=255)
                                                                          gcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGttt                                                                     <  1:788885‑M2/75‑1 (MQ=255)
                                                                                cattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTTGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGc                                                               >  1:557467‑M2/63‑143 (MQ=255)
                                                                                cattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTAGAACTTCCGGAACCTGGAGGGGGAAACCCAACTCACGTTGTTTACCGTTCGCCTGc                                                               >  1:198913‑M2/63‑143 (MQ=255)
                                                                                      agccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGCTTGACTGCTCGCAg                                                         >  2:903386‑M2/57‑143 (MQ=255)
                                                                                       gccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTGACCGTTTGACTGCTCGCAGa                                                        >  1:300337‑M2/56‑143 (MQ=255)
                                                                                       gcagcgagcgtatatcgactggctgacgctggcgtgcgggtaggcgatacgtggcAGCGGTACGGGAATGTGTATTATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATGCACCTTTTTTACCGTTTGACTGCTCGCAGa                                                        <  2:754997‑M2/88‑1 (MQ=255)
                                                                                         caccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGatc                                                      >  2:1034607‑M2/54‑143 (MQ=255)
                                                                                         caccagcgtaaaacaacgggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGatc                                                      <  2:692629‑M2/90‑1 (MQ=255)
                                                                                          accagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAGACAATACCACCTCTCTTACCGTTTGACTCCTCGCAGacca                                                     >  2:769899‑M2/53‑143 (MQ=255)
                                                                                            cagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATCTTCCAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGatcatc                                                   <  1:630826‑M2/93‑1 (MQ=255)
                                                                                                 taaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGaa                                              >  1:1053998‑M2/46‑143 (MQ=255)
                                                                                                   aaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTCTTACCGTTTGACTGCTCGCAGATCATCGGGAacg                                            >  2:343273‑M2/44‑143 (MQ=255)
                                                                                                    aacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAacga                                           >  1:592693‑M2/43‑143 (MQ=255)
                                                                                                          tgcctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCg                                     <  1:89724‑M2/107‑1 (MQ=255)
                                                                                                              tgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAATGGCACGCGCTCa                                 >  1:144384‑M2/33‑143 (MQ=255)
                                                                                                                     gggctgggggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCAt                          <  2:90722‑M2/118‑1 (MQ=255)
                                                                                                                     ggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCCTTGCcac                          >  2:840963‑M2/26‑142 (MQ=255)
                                                                                                                      gcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTGGCAGGTCATCTGGAGCGACGCACGGGTATTGCCAtt                         >  1:996485‑M2/25‑143 (MQ=255)
                                                                                                                      gcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACATCAGGAACCTGGAACGCGAAAACCAATTCACCTTTTTTACCGTTTGAATGCTCGAAAATCAACTGGAAAGAAGCCCGCTCATTGCCAtt                         >  2:700133‑M2/25‑143 (MQ=255)
                                                                                                                              gtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAATCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAAt                 <  2:399751‑M2/127‑1 (MQ=255)
                                                                                                                                   cgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACAGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGaa            <  2:954122‑M2/132‑1 (MQ=255)
                                                                                                                                    gatacattgcAGCTGTACGGCAATGTGTAATATTCTAACTTCCGGAACCTAGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAAc           <  1:847106‑M2/133‑1 (MQ=255)
                                                                                                                                       acattgcAGCGGTACGGCAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCCCCTTTTCTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTgg        <  2:1054854‑M2/136‑1 (MQ=255)
                                                                                                                                         cttgcAGCGGGTCGGGAATGTGTAATATTCGAACTTCCGGACCCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTc      <  2:349287‑M2/138‑1 (MQ=255)
                                                                                                                                         attgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTc      >  1:260093‑M2/6‑143 (MQ=255)
                                                                                                                                         attgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTc      >  2:653717‑M2/6‑143 (MQ=255)
                                                                                                                                          ttgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTca     <  1:764949‑M2/139‑1 (MQ=255)
                                                                                                                                           tgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAACTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTCCCAGAATCTGACCTGGCcaa    >  1:429252‑M2/4‑143 (MQ=255)
                                                                                                                                             cAGCGGTACGGGAATGTGTCATATGCGAACTCCCGGGACCTGGAGCGCGAAACCCAATTCACCTTTTTGACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaaca  <  1:427915‑M2/142‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                             
GTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACA  >  NC_000913/3527521‑3527804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: