| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,316,393 | T→C | Y73H (TAC→CAC) | rcsB → | response regulator in two‑component regulatory system with RcsC and YojN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,316,393 | 0 | T | C | 95.2% | 172.3 / NA | 63 | Y73H (TAC→CAC) | rcsB | response regulator in two‑component regulatory system with RcsC and YojN |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (31/28); minor base A (1/2); total (33/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGATCTGCCGAAAGC > NC_000913/2316258‑2316532 | tGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAGACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGCACGGCGATGGCATTACCATAATCAAGCACATaaa > 2:179899/1‑143 (MQ=255) gTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCACCAACCTGCCGAAACTGGATGCGCATGTGCTGATTACCGCTCCCCCCACACCTGGCCATAACTCCGGCGAACGCATCACCGTAGCCAAGCACATCCAgcg > 1:220615/1‑143 (MQ=255) gTGAATGTCGTCGCCTAATTTGAAGACTCTACACCACTGACCAACACCCTGCCGAACCTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAgcg < 1:1036811/143‑1 (MQ=255) aTGCTGTCGGCGAATTTGAAGACCCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCGGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCAt < 1:468259/143‑1 (MQ=255) tgttgtCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCAtt > 1:465954/1‑143 (MQ=255) ttgtCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCCAGCACATGAAGCGCCAtctc > 2:952415/1‑143 (MQ=255) ttgtCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCCTTTc > 2:302599/1‑143 (MQ=255) tgtCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGTTGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTcc < 1:440966/143‑1 (MQ=255) cGGCGTATTTGAAGGCTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCaa < 2:463684/143‑1 (MQ=255) gAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCCATGGCATTACCTTAATCAAGAACATCAAACGCCACTTCCCAACCCt > 2:407226/1‑143 (MQ=255) tttGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTATTCCTGCGCATCAAGCGGCATTTCCTAAGCCTGTc > 1:10369/1‑143 (MQ=255) gAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCGTAATCAAGCACATCAAGCGCCACTTCCCAAGCCTGTCGac > 2:1072385/1‑142 (MQ=255) ctACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGtt < 1:879845/143‑1 (MQ=255) ggCACTGATCAACCACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGa < 2:348231/142‑1 (MQ=255) cACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCATGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTGGATGATTGGTCTGACt > 2:1058211/1‑143 (MQ=255) tGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATg > 2:1003872/1‑143 (MQ=255) tGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATg < 2:326114/143‑1 (MQ=255) tGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATg > 2:554481/1‑143 (MQ=255) tGAGCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATg < 2:643738/143‑1 (MQ=255) caacaaCCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGaaca < 1:232021/143‑1 (MQ=255) aacaacCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCCCACAACGTGCCATTTCCCCCGCACGTCGATCATTGTTCTGACTATGaacaa > 2:650767/1‑143 (MQ=255) aaCTGGGCGCGCATGTGTTGATTACCGGTCTCGCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGAtt < 2:104766/143‑1 (MQ=255) tGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACACGGCGATTCtt > 2:95544/1‑143 (MQ=255) ggATGCGCATGTGTTGATTACCGATCGCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTa < 2:470490/143‑1 (MQ=255) gATGCGCATGTGTTGATTACCGTTCTCGCCATGCTTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGGTCATTGTTCTGACTATGAACAACATCCCGGCGATTCTTAg < 2:922885/143‑1 (MQ=255) tgagtagATTACCGATCTATCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATTAATCACATCAATCGCCATTATCAAAGCCTGTCGATCATTGGTCGGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTAt < 1:177434/137‑1 (MQ=255) gtgtTGATTACCGATCGCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTAtt < 1:933795/143‑1 (MQ=255) gATTACCGATCTCTCCATGCCCGGCGATAAGTACGGCGATGGCATTACCTTAACCAAGCACATCAAGCGCCATTTCCCAAGCCGGTCGATCATTGTTCTGACTATGAACAACAACCCGGCTATTCTTAGTGCGGTATTGGATc < 1:332966/143‑1 (MQ=255) tACCGCTCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCCAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTgg < 1:887169/143‑1 (MQ=255) cGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCACGAGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGata > 2:1012866/1‑143 (MQ=255) cGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGGGGTGTTGGATCTGGata > 2:985853/1‑143 (MQ=255) aTCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTACCAAGACTGTCGATCATTGTTCTGACGATGAACAACACCCCGGCGATTCTTAGTGCGGCAATGGCGCTGGatagc > 2:635802/1‑141 (MQ=255) tcgccATGCTTGGCGATAAGTACGGCGATGGCCTTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGaa < 2:66132/140‑1 (MQ=255) actcccggCCTCGCGATAAGTACGGCTATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGaa < 1:347364/136‑1 (MQ=255) tgaaTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCGCATCAAGCGCCATGTGCCAAGCCTGTCGATCACTGTTCAGACTATGGACAAGAAGCCGGTGAGTGTTAGTGCGGAGTTGGGTCCGGATATCGCAgg > 2:584194/4‑143 (MQ=255) tGCCTGGCGATAAGTACGGCGTTGGCATAGTGGTTATCAAGAACTCCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATc < 2:10369/143‑1 (MQ=255) gCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTAGATCATTGTTCTGACTATGAACAACAACCCGGAGATTCTTAGTGCGGGATTGGAACTGGATATCGAAGGGAACg > 2:505489/1‑143 (MQ=255) tGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGc > 1:730249/1‑143 (MQ=255) tGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGc < 1:554481/143‑1 (MQ=255) ggCGAGAAGTACAGGGATGGCATGACCGAAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGTTATTGGATCTGGATATCGAAGGGATCGTGCt < 1:312420/143‑1 (MQ=255) gCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCCTTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGAATTGGACCTGGATATCGAAGGGATCGTGCTg > 2:507458/1‑143 (MQ=255) gATACGTGCGGGAATGGGATTCGCGTACTCAAGAACATGAGTCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGAGTATAAACAACAACCCGGCGATTCTTAGTGCGGTATAGGATCGGGATATCGAAGGGATCGTGCTGaa < 2:928539/143‑1 (MQ=255) aatcaGTACGGCGATGGCATTCCCTTGATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTGCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGaa < 1:329556/139‑1 (MQ=255) aaGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACCATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGCTATCGAAGGGATCGTGCGGAAACa > 2:609011/1‑143 (MQ=255) aaGTACGGCGATGGCATTAACTTAAACAAGGCCATCAAGCGCCATTGGCCAAGCCTGTCGATCATTGTTATGACTATGAACAACAACCCGGCGACTCTTAGTGCGGTATTGGATCTGGCTATCGAAGGGATGGTCCTGAAACa > 2:667028/1‑143 (MQ=255) aGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGACACaa > 2:740958/1‑143 (MQ=255) ggaCGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAg < 1:861501/141‑1 (MQ=255) gTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAg < 1:512082/143‑1 (MQ=255) tACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGATCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAgg > 2:338892/1‑143 (MQ=255) acctttaTGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGt < 1:547103/137‑1 (MQ=255) ggCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGc > 2:812921/1‑143 (MQ=255) aTGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGCTCGTGCTGAAACAAGGTGCaccg > 1:999964/1‑143 (MQ=255) aTGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCaccg > 1:1075824/1‑143 (MQ=255) ggCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCaccgac < 1:1006305/143‑1 (MQ=255) gCATTACCTTAATCAGGCACAGCAAGCGCCATTCCCCAGGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCaccgacc < 2:987952/143‑1 (MQ=255) gCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGACACACGGTGCcccgacc > 1:679460/1‑143 (MQ=255) cATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAATCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCaccgaccg < 1:407226/143‑1 (MQ=255) aTTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTCTTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCAccgaccga > 2:773198/1‑143 (MQ=255) cTTAATCCAGCACATCCAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGTCGATTTTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGATCTGc < 1:98840/143‑1 (MQ=255) cTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGCCTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATACCGAAGGGATCGTGCTGAAACAGGGTGCACCGACCGATCGGc > 1:596060/1‑143 (MQ=255) tCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGGCCGAGCTGCCGaaa > 1:167545/1‑143 (MQ=255) aaGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGCACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGGTATCGACGGGATCGTGCTGAAACCAGGTGCATCGGCCGTGCTGCCCaagcc > 1:90127/1‑140 (MQ=255) aaGCACAGCAAGCGCCATTTCCCAAGCCTGTCGATCATTGATCTGACGATGAACAACAACCCCGCGAGTCGTAGCGCGGCATTGGATCTGGAACCCCAAGCGAGCGTCCTCAAACCACGAGCACCGAGCGACCACCCGaagcc > 2:39295/1‑140 (MQ=255) | TGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGATCTGCCGAAAGC > NC_000913/2316258‑2316532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |