Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	45 [0]	[0] 45	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	43 (1.020)	35/276	0.2	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

GCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGG  >  NC_000913/1300562‑1300839                                                                                                                                         |                                                                                                                                              agttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataa                                                                                                                                         <  1:771632‑M2/7‑1 (MQ=255)   ttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgatgaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATc                                                                                                                                       >  2:634193‑M2/135‑143 (MQ=255)       ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                   <  1:217502‑M2/13‑1 (MQ=255)       ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                   <  2:446824‑M2/13‑1 (MQ=255)       ttataaggatttcctgaactgcttcgccgcaccatcatgttattttcgccacatcataagcctgggcttgctgaagaataattgaaatgatattagtaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                   <  1:68654‑M2/13‑1 (MQ=255)              gatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAat                                                                                                                            >  1:327955‑M2/124‑143 (MQ=255)                   ccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtg                                                                                                                       <  2:76790‑M2/25‑1 (MQ=255)                     ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgtg                                                                                                                     >  2:140877‑M2/117‑143 (MQ=255)                          tgcttctcctcaccatcatggtattttcgccacatcataatcctgggcttgctgaagaataattgaaaggatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                <  2:128027‑M2/32‑1 (MQ=255)                            cttctcctcaccgtcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCa                                                                                                              <  2:218275‑M2/34‑1 (MQ=255)                            cttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCa                                                                                                              <  1:338400‑M2/34‑1 (MQ=255)                             ttctcctgaccatcatgttgttttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCac                                                                                                             <  2:562250‑M2/35‑1 (MQ=255)                             ttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCac                                                                                                             <  1:763700‑M2/35‑1 (MQ=255)                               ctcctctccatgtggtaactttctccagatcataattctgggcttgctgaagaagaattgaaatgatattattaatttcactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCacta                                                                                                           <  2:507627‑M2/37‑1 (MQ=255)                               ctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCacta                                                                                                           <  2:566205‑M2/37‑1 (MQ=255)                                tcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgacatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCactac                                                                                                          >  1:704613‑M2/106‑143 (MQ=255)                                 cctcgccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCactact                                                                                                         <  2:342648‑M2/39‑1 (MQ=255)                                     accatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGaa                                                                                                     >  2:743769‑M2/101‑143 (MQ=255)                                       catcgtgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACt                                                                                                   <  1:412485‑M2/45‑1 (MQ=255)                                              ttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatcttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTACATTATAGTGCATTTCACTACTGGACCTGTAATCa                                                                                            >  2:443425‑M2/92‑143 (MQ=255)                                                   ttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccattgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaaa                                                                                       >  1:495662‑M2/87‑143 (MQ=255)                                                       ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaatacaactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGAAACTGTAATCAGAAGAGATa                                                                                   >  1:646606‑M2/83‑143 (MQ=255)                                                        cacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAg                                                                                  <  1:230769‑M2/62‑1 (MQ=255)                                                        cacatcataatcctgggcttgctcaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAg                                                                                  <  1:67120‑M2/62‑1 (MQ=255)                                                         acatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGa                                                                                 >  1:254049‑M2/81‑143 (MQ=255)                                                                   cctgggcttgctgaagagtaattgaaatgatattattgattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGc                                                                       <  2:643643‑M2/73‑1 (MQ=255)                                                                            gctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTa                                                              <  2:100187‑M2/82‑1 (MQ=255)                                                                              tgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAtt                                                            >  2:634433‑M2/60‑143 (MQ=255)                                                                                 agaataattgaaatgatattagtaattccactgtctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttga                                                         <  2:137966‑M2/87‑1 (MQ=255)                                                                                  gaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgat                                                        <  1:552071‑M2/88‑1 (MQ=255)                                                                                   aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                       <  1:427685‑M2/89‑1 (MQ=255)                                                                                            aatgatattattaagtccactgccgttggtagaggaaagtgcttgTTTATAATCAATTGTTAAATTATGGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAGAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                              <  1:532310‑M2/98‑1 (MQ=255)                                                                                                         attccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAACGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                                 >  2:127646‑M2/33‑143 (MQ=255)                                                                                                              actgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATg                            <  1:594506‑M2/116‑1 (MQ=255)                                                                                                              actgcatttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTTCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATg                            >  1:662880‑M2/28‑143 (MQ=255)                                                                                                                      tggtagaggaaagtgctaaATAATAATCAATTGTTAAATAATGGTGCATTACACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATATGATAGAATAGAAAGAAGTTAGATAAGCCACGAATGCAAg                    >  2:546386‑M2/20‑143 (MQ=255)                                                                                                                       gttagaggaaagttataaATAATAATCAATTGATAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAACATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGc                   <  2:719798‑M2/125‑1 (MQ=255)                                                                                                                         tagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCta                 >  1:540968‑M2/17‑143 (MQ=255)                                                                                                                           gaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtata               >  1:686019‑M2/15‑143 (MQ=255)                                                                                                                           gaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtata               >  1:669861‑M2/15‑143 (MQ=255)                                                                                                                           gaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtata               <  1:10606‑M2/129‑1 (MQ=255)                                                                                                                            aggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAg              >  1:366773‑M2/14‑143 (MQ=255)                                                                                                                            aggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACAGGCTTAGCCAATCCTAATATTATTTATTTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAg              >  1:743508‑M2/14‑143 (MQ=255)                                                                                                                                       aaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACg   >  2:100793‑M2/3‑143 (MQ=255)                                                                                                                                        aATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACgg  <  2:254049‑M2/142‑1 (MQ=255)                                                                                                                                         |                                                                                                                                              GCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGG  >  NC_000913/1300562‑1300839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑