Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,308,104 G→A A274V (GCA→GTA)  nlpI ← lipoprotein involved in osmotic sensitivity and filamentation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,308,1040GA97.8% 117.0 / NA 46A274V (GCA→GTA) nlpIlipoprotein involved in osmotic sensitivity and filamentation
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (22/22);  minor base C (1/0);  total (24/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGC  >  NC_000913/3307964‑3308241
                                                                                                                                            |                                                                                                                                         
tATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGCTCCTGGCCCCGGAGCGATAACTCCAACCATCCg                                                                                                                                         >  2:612859/1‑143 (MQ=255)
tATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCCACCCGCTAACGCTTGTGCTGGCCCCGGAGCGATCACTCCAACAATACg                                                                                                                                         >  2:161765/1‑143 (MQ=255)
   tAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGGCCAGGAGCGATAATTCCAACAATACGTAt                                                                                                                                      >  1:327317/1‑143 (MQ=255)
        aacaacgacTCCCTCCCCCGTTAAGGTGATGGCAATCAAAAAAGACGACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTg                                                                                                                                 <  1:22704/143‑1 (MQ=255)
          caacaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGGCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCt                                                                                                                               <  2:354559/143‑1 (MQ=255)
           aacaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTc                                                                                                                              >  1:2500/1‑143 (MQ=255)
                    cTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGtt                                                                                                                     <  1:96540/143‑1 (MQ=255)
                     tCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTa                                                                                                                    <  1:243398/143‑1 (MQ=255)
                         ccGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCGAACAATACGTATCCGTCGTGAACAAAGTTATGaa                                                                                                                >  1:646880/1‑143 (MQ=255)
                                 gTGAGGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGttgttg                                                                                                        <  2:337563/143‑1 (MQ=255)
                                         aaTCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACCAAGTTATGAACGTTGTTGGCAACCGc                                                                                                >  2:283418/1‑143 (MQ=255)
                                         aaTCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAACCCCAACAATACGTATCGGTGCCCAACAAAGTTATGAACGTTGTTCGCAACGGc                                                                                                >  2:677407/1‑143 (MQ=255)
                                         aaTCAAAAAAGATTAAGGGCTGATGTGTACGTCAGCTATTGATGGTCCGATTATGGCAGGTCATGTTGGTCCTGGCCCAGGAGCGATAATTTCAACAATACGTATGGGTGACACAGAAAGTCACGCACGTAGTTGGCAACCGc                                                                                                >  2:733598/1‑143 (MQ=255)
                                              aaaaaGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGtt                                                                                           >  1:134785/1‑143 (MQ=255)
                                              aaaaaGATTACGGGCTGATGGGTACGTCAGCTAATGCTGGTCCGATTCTGCCAGGTCAGCTTGGTCCGGGCCCAGGAGCGATCATCCCAACAATGCGTAGCGGTGCCCAACACAGCTAAGGACGTTGTTGCCAACGGccggag                                                                                           >  2:393193/1‑139 (MQ=255)
                                               aaaaGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGttt                                                                                          <  1:372023/143‑1 (MQ=255)
                                                 cggATTACGGGCTGATGTGTCCGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCACAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGa                                                                                        <  1:296255/141‑1 (MQ=255)
                                                     ttACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAg                                                                                    >  1:513448/1‑143 (MQ=255)
                                                           gCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCGGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCGGt                                                                              >  2:559137/1‑143 (MQ=255)
                                                            cTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTg                                                                             <  1:430451/143‑1 (MQ=255)
                                                            cTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTg                                                                             <  2:106282/143‑1 (MQ=255)
                                                               aTGTGTACGTTAGCTATTGTTGGTCGTGTTCTGCTAGGTCATCTTGGTCCTGGCTCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGgcg                                                                          <  2:585493/143‑1 (MQ=255)
                                                                   gtACGTCAGCTAGCGCTGGTGCGATTCTGCCAGGTCATCTTGGTCCTGGCGCAGGAGCGATAGTTCCAACAATACGTATCGGTGCTCAACAAAGTTGTGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGt                                                                      <  2:517212/143‑1 (MQ=255)
                                                                    tACGTCGGCTATTGCTGGTCCGGTTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTc                                                                     <  2:141826/143‑1 (MQ=255)
                                                                        tCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTGTCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCaaa                                                                 >  2:416839/1‑143 (MQ=255)
                                                                         cAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGCTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAAt                                                                >  2:367554/1‑143 (MQ=255)
                                                                           gCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGGGGCGCTGTCCGAATcc                                                              >  1:680529/1‑143 (MQ=255)
                                                                                   tGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACt                                                      >  1:82277/1‑143 (MQ=255)
                                                                                     gTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTa                                                    >  1:418011/1‑143 (MQ=255)
                                                                                         gATTCTGCCAGGTCATCTTGGTCCTGGCCCGGGAGCGATAGTTCCAACAATACGTATCGGTGCTCAACAAAGTTATGGACGTTGTTGGCAACCGCCAGATTGGACAGGGCCGTGGCGCTGTGAAAATCCCCCCGACTTCGgta                                                >  1:629214/1‑143 (MQ=255)
                                                                                            tCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGgtagta                                             >  1:346072/1‑143 (MQ=255)
                                                                                             cTGCCAGGTCTGCTTGGTCCGGGGCCAGGAGCGATAATTCCAGCAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTAc                                            <  2:296978/143‑1 (MQ=255)
                                                                                               gCCAGTTCATCTTGGTCCTGGTCCCGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACtt                                          <  2:301213/143‑1 (MQ=255)
                                                                                                 cAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACAGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCGCCAGACTTAGGTAGTACTTAc                                        >  1:461967/1‑143 (MQ=255)
                                                                                                  cggccATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAAAAGTGCCGTGGCGCTGTCCAAATCCCCCACACTTAGGTAGTACTTaac                                       >  2:136136/5‑141 (MQ=255)
                                                                                                       aTCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAAAAAAGTTATCAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGCGGCGCTGTCCAACTCCCCCAGACTTAGGTCGCACTCACTTAAAt                                  >  2:356892/1‑143 (MQ=255)
                                                                                                           tGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTAACGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGaa                              <  1:384282/143‑1 (MQ=255)
                                                                                                               ctcggCGCAGGAGCGATAATTCCAAGAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTg                          <  2:452948/140‑1 (MQ=255)
                                                                                                                cTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGCAGTTgg                         >  1:716069/1‑143 (MQ=255)
                                                                                                                         gAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTCGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTga                <  1:589334/143‑1 (MQ=255)
                                                                                                                           gCGATAATTCCAACATTACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCTGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTgaga              <  2:134407/143‑1 (MQ=255)
                                                                                                                           gCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTgcga              >  2:727854/1‑143 (MQ=255)
                                                                                                                           gCGATAAGTCCAACAATACGTATCGGTGCGCAACAAAGGTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTgaga              <  1:183354/143‑1 (MQ=255)
                                                                                                                                     cagcaaTACGTATCGGCGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCagcga    <  2:312363/140‑1 (MQ=255)
                                                                                                                                       ccaaTACGTATCGGGGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCagcgagc  <  2:275657/142‑1 (MQ=255)
                                                                                                                                       acaaTACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCagcgagc  <  2:82277/143‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                         
TATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGC  >  NC_000913/3307964‑3308241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑