| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,316,760 | T→C | V195A (GTC→GCC) | rcsB → | response regulator in two‑component regulatory system with RcsC and YojN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,316,760 | 0 | T | C | 94.6% | 85.5 / ‑6.2 | 38 | V195A (GTC→GCC) | rcsB | response regulator in two‑component regulatory system with RcsC and YojN |
| Reads supporting (aligned to +/- strand): ref base T (0/1); major base C (21/14); minor base A (0/2); total (21/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
TCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGG > NC_000913/2316628‑2316887 | tctcGCCAAAAGAGAGTGAAGTTCTGCGCTTGCTTGCGGAATTCGTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTCTTAAAACCATCAGGAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGat > 2:277408/1‑143 (MQ=255) gCCAAAGGAGAGTGAAGTTCTGCGCCTGTGTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCg < 1:692612/143‑1 (MQ=255) ccAAAAGAGAGAGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACAGCAGTATTCACACCATCAGTAGCCAGACGACACCTGCGATGAGGAAGCTGGGTGCCGAGAACGATATCGc > 2:271046/1‑143 (MQ=255) gAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATGGCCctgctgcagtg > 1:14371/1‑138 (MQ=255) cgcCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTc > 1:565083/1‑143 (MQ=255) tgttgcttGGGGAAGGCCTTCTGGTGGCCGGGAAAAGTCAAAAGCTGTACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCa < 2:344288/137‑1 (MQ=255) gcCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCa < 2:649597/143‑1 (MQ=255) cTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAACCGATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGCGCAGAGAACGATCTCGCCATGCTGAATTATCTCGCTTCAgg > 2:26825/1‑142 (MQ=255) cTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGt < 2:742031/143‑1 (MQ=255) ttctgcaaGGCTGCCGGGTGCCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGAcc < 2:582521/137‑1 (MQ=255) ggAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGCCCTTaa > 1:716605/1‑143 (MQ=255) gAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGCTGATGAAGCTGGGTGCCGAGCACGACATCGCCCTCCTGAATTAACTCCCCTCAGTGCCCtaccg > 2:522499/1‑139 (MQ=255) ttCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGc > 2:47454/1‑143 (MQ=255) ttCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGc < 1:663452/143‑1 (MQ=255) ttCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGc < 1:99405/143‑1 (MQ=255) ttCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCAGCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCGCTCTTCAGTGACCTTAAGTCCGGc > 2:94600/1‑143 (MQ=255) ccTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCCGCGAGACTGAAGCTAGGTGCCGTGAACGAACACGCCCTGCTGAATTAGCTCTCTGCAACGACCTTAAGTCCGGCAg > 2:130623/1‑143 (MQ=255) cTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCGATGGTGAATTATCTCTATTAAGTGAGATTAAGTCGAGCAGa > 2:275999/1‑143 (MQ=255) ggTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGAAGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGACCGGCAGATa > 2:290915/1‑143 (MQ=255) aCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGa > 1:773532/1‑143 (MQ=255) aCCGAGATCGCTAAAAAGCTGAACCCTATTATGAACACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGa < 1:529871/143‑1 (MQ=255) ggcgaagaaGCGGAACCGCAGGATGAAAACCATCAGGAGCCAGAAGCAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCAc < 2:218331/136‑1 (MQ=255) cGCTAAAAAGCTGAACCGCAGTATTAACACCATCAGTAGCCAGAAGAAAGCGGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCAc < 2:357859/143‑1 (MQ=255) cGCTAAAAAGCCGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCAc < 1:585392/143‑1 (MQ=255) tAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGGCTTATCGCTCTTCAGTGCCCTTAAGTCCGGCAGATAACGACTCATGAcccg > 1:32457/1‑141 (MQ=255) aatgCTGAACCGCAGTATTAAAACCATCTGTATCCAGAAGAACTCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAg < 2:379100/140‑1 (MQ=255) aaaGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATACAGACTAATCACCTGTAg > 1:92452/1‑143 (MQ=255) aaGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCGGATAAAGACTAATCACCTGTAgg > 1:313760/1‑143 (MQ=255) cTGGACCGCAGTATGCAATGCATTTGTGGCCAGAAGAAATCTGCGATGATGTAGCTGGGTGACGAGAACGCTATCGCCCTGCTGAATTATCTCTCTTCAGTGTCCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCa < 2:8855/143‑1 (MQ=255) aaCCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTGGGCCAGAt > 2:393707/1‑143 (MQ=255) ccGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAACGACTAATCCCCTGTAGGCGAGGTaa > 2:536520/1‑143 (MQ=255) ccGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATaa > 1:505330/1‑143 (MQ=255) cAGTAGTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGCGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGAc < 1:604827/143‑1 (MQ=255) tATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCGTCAGTGACCTTAAGTCCGGCAGATAAAGACGAAGCACCTGTAGGACAGATAAGAcgcg > 2:694584/1‑143 (MQ=255) cATCAGTAGCCAGAAGAATTCGGCGATGATGAAGCTGGGTGCCGAGAACAATACCGCCCTGCTGAATTATTTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCt < 1:362164/143‑1 (MQ=255) agaagaAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGCGTCTTATCTGGCAtt > 2:336387/1‑143 (MQ=255) gatgatGAAGCTGGGTGACGAGAGTAATGTTGCCCTGCTGAATTACCTCTCTACAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGcc < 1:489244/143‑1 (MQ=255) tgatcaAGGTGGGGGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCTGGTAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCgg < 2:14371/143‑1 (MQ=255) | TCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGG > NC_000913/2316628‑2316887 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |