Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	4,001,645	Δ5 bp	coding (220‑224/951 nt)	corA →	magnesium/nickel/cobalt transporter

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	4001645	4001649	5	29 [0]	[0] 29	corA	magnesium/nickel/cobalt transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4001644	0 (0.000)	29 (1.170)	25/512	0.1	100%	coding (219/951 nt)	corA	magnesium/nickel/cobalt transporter
	?	NC_000913	4001650 =	0 (0.000)					coding (225/951 nt)	corA	magnesium/nickel/cobalt transporter

CGATATTTTAAGCATTGGGAGTCCCGGTCATGCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGA  >  NC_000913/4001397‑4001886                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   cGATATTTTAAGCATTGGGAGTCCCGGTCATGCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcg                                                                                                                                                                                                                           <  1:123064‑M1/273‑26 (MQ=255) cGATATTTTAAGCATTGGGAGTCCCGGTCATGCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcg                                                                                                                                                                                                                           >  2:123063‑M1/1‑248 (MQ=255)  gATATTTTAAGCATTGGGAGTCCCGGTCATGCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCACctccttcttcttctttgaagatgcggca                                                                                                                                                                                                                        >  2:160567‑M1/1‑247 (MQ=255)         tAAGCATTGGGAGTCCCGGTCATGCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCCACCCGCCCGGACCTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctcattcttcttctttgaagatgtggaagatc                                                                                                                                                                                                                    >  1:84946‑M1/1‑240 (MQ=255)           aGCATTGGGAGTCCCGGTCATGCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCCCGTTTCTTGGAAGACGACGACGGCCTGAATctccttcttcttctttgaagatgcggaagatcacgcc                                                                                                                                                                                                               >  1:96666‑M1/1‑238 (MQ=255)                tGGGAGTCCCGGTCATGCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATccccttcttcttctttgaagtttcggaagatcaagccggtaa                                                                                                                                                                                                          >  2:76206‑M1/1‑233 (MQ=255)                       cacggTCATGCTGAGCGCATTTCAACTGGGAAATAACCGACTGACCCGGCTGGAAGTGGAAGAGTCACAACCCCTTGTAAAGGCAGGATGGAGTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccact                                                                                                                                                                                                   <  2:114783‑M1/273‑50 (MQ=255)                                    aGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgacggtaactccactgtggcatttacc                                                                                                                                                                                       >  1:87189‑M1/1‑213 (MQ=255)                                           ttCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATccccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtg                                                                                                                                                                                >  1:191479‑M1/1‑206 (MQ=255)                                                 tGGCAAATAACCGACTGACCCGGCTTGAAGTCGAAGGGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcg                                                                                                                                                                         <  2:92451‑M1/275‑76 (MQ=255)                                                 tGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcg                                                                                                                                                                         <  1:184366‑M1/275‑76 (MQ=255)                                                              aCTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTCTCTTTGAAGACGACGACGGCCTGCATctccttcttcttcttttaaggttcggaagataacgccggtaactccactgtggcctttcccatccgtgatggttgtctgtttacg                                                                                                                                                               >  1:195658‑M1/1‑187 (MQ=255)                                                                        cTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttcctctgcgtgcgcttg                                                                                                                                                  >  1:229146‑M1/1‑177 (MQ=255)                                                                                       tCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgctttt                                                                                                                                    <  1:57293‑M1/274‑113 (MQ=255)                                                                                                  tGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtcggtttactctgcgtgagcgtgaactgcccgcttttcgtcggtatcg                                                                                                                         >  1:48103‑M1/1‑151 (MQ=255)                                                                                                        tGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCCCGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctggttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgt                                                                                                                  >  1:174715‑M1/1‑145 (MQ=255)                                                                                                                  aTTGATCTTGGCGAACCGGACGCCGACGAGCGGCTGCGCGGACAATCTGAACTTGGCCAGAGCCGGGCAACACGCCCGGAACTGGAAGACATTGAAGCATCGGCACGTTTCTTTGAAGACGACGGCGTCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcctgcttttcgtctgtatcgtatgcgtgcccgtagc                                                                                                         <  1:160568‑M1/274‑140 (MQ=255)                                                                                                                    tGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcccgccgttaactcccctgtggcatttaccttccctcatggtcgtctttttaccctccgtgaacacgaactgcccgcttttcttctgtttgttatgcgtgcccttggccag                                                                                                      >  1:122983‑M1/1‑133 (MQ=255)                                                                                                                         ttGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatg                                                                                                                                                                             >  2:86031‑M1/1‑128 (MQ=255)                                                                                                                           gTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgat                                                                                                 >  1:96463‑M1/1‑126 (MQ=255)                                                                                                                           gTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgccagtagccagtcgat                                                                                                 >  1:65744‑M1/1‑126 (MQ=255)                                                                                                                            tCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatg                                                                                                                                                                             >  2:207631‑M1/1‑125 (MQ=255)                                                                                                                             cGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcg                                                                                                                                                                         <  1:86032‑M1/199‑76 (MQ=255)                                                                                                                                                            aaatCGGAACTTGGCCAGGGCCGGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgatggtagacggtaacgcctacgagttgctgctggat                                                               <  2:191478‑M1/273‑182 (MQ=255)                                                                                                                                                                                                     ggAAGACACTGCAGCAGCGGCAGCTATCTTTGAAGCCGACGCCGGCCGTCATctcctgcttcttctttgcagacgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgatggtagacggtaacgcctacgagttgctgctggatctgttcgaaaccaaaatcgaacagttggcagatgaaattgaa                     <  1:76207‑M1/275‑224 (MQ=255)                                                                                                                                                                                                                aggcaTCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgatggtagacggtaacgcctacgagttgctgctggatctgttcgaaaccaaaatcgaacagttggcagatgaaattgaaaatatctatag          >  1:66116‑M1/3‑41 (MQ=255)                                                                                                                                                                                                                aaGCATCGGCCCGGTTCTTTGAAGACGACGACGGCCTGCATctccttctgcttcgttgaagatgcggaagatcacgccggtaaatccactggggcatttaccatccgtgatggtcgtctgttgacgctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgatggtagacggtaacgcctacgagttgctgctggatctgttcgaaaccaaaatcgaacagttggcagatgaaattgaaaatatctatag          <  2:122982‑M1/275‑235 (MQ=255)                                                                                                                                                                                                                aaGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgaggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagcccgtcgatggtagacggtaacgcctacgagttgctgctggatctgttcgaaaccaaaagcgaacggttggcagatgaacttgaaaatatctatag          >  1:152068‑M1/1‑41 (MQ=255)                                                                                                                                                                                                                          aCGTTTCTTTGAAGGCGACGACCGCCTCCAGctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgatggtagacggtaacgcctacgagttgctgctggatctgttcgaaaccaaaatcgaacagttggcagatgaaattgaaaatatctatagcgacctgg  <  2:174714‑M1/273‑243 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   CGATATTTTAAGCATTGGGAGTCCCGGTCATGCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGA  >  NC_000913/4001397‑4001886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑