Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,560,4551.G100.0% 69.3 / NA 23intergenic (‑2/+1)glpR/glpRpseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (10/13);  total (10/13)

AGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC  >  NC_000913/3560323‑3560580
                                                                                                                                     |                                                                                                                             
aGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAg                                                                                                                      <  1:609950/143‑1 (MQ=255)
         ccATTGGGGATTCGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTc                                                                                                             <  1:269480/143‑1 (MQ=255)
          cgttGGGGATGTGCTCCGCCACTTTCCGGGCGTGGCGCTCTTTTGCTTCGGTCTGGGTGGCCTGGCGATCGTTCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATTGCGCAGGATCAGGTTTTGCTcc                                                                                                            <  2:201863/141‑1 (MQ=255)
               ggcaTTTGCTCCGCCACTTTGCGGGCGATGCGCGCGTGTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAg                                                                                                       <  1:85686/140‑1 (MQ=255)
                   gtctcTCCGCCACTTTGCGGGCGATGCGCTCGTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCa                                                                                                   <  1:394108/139‑1 (MQ=255)
                                                 ctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGTCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTGTTGCTCCGCCAGCTCATTGAGGTCCCGGCGAATAGTATGCGGGCTg                                                                     >  2:127720/1‑143 (MQ=255)
                                                        ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAACAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGAGAATAGACCGCGGGCTGACGgacg                                                              >  2:35912/1‑141 (MQ=255)
                                                        ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGACGGCGCAGGATCAGGTTTTGCTCCGCCAGCGCATCGAGGTCGCGGCGAATAGTCGGCGGGCTGACGgaga                                                              >  2:546765/1‑143 (MQ=255)
                                                         tcggtcggGGTGGCCGTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaa                                                             <  2:452050/143‑1 (MQ=255)
                                                         tcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaa                                                             >  1:542478/1‑143 (MQ=255)
                                                           ggTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGAGGCAGCGGGGAATAGTCTGCGGGGTGAGGgaggcaa                                                           >  2:350085/1‑139 (MQ=255)
                                                                     gCCTTGCGATCGTGCCGCGGCGTGTTAACCGTACTGGAAGGCAGCGCCGCACCGCCATGTTGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg                                                 <  2:341289/143‑1 (MQ=255)
                                                                         tGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGGTCAGGTTTTGCTCCGCCAGCCCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGCAATGCTCTACCAGctct                                             >  1:642569/1‑143 (MQ=255)
                                                                                            gtTAGCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                          <  1:448274/143‑1 (MQ=255)
                                                                                              tAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgc                        <  2:243553/143‑1 (MQ=255)
                                                                                              tAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCCCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAGGCTCTCCCAGCGCTTCGGTACGGACATAACCccgc                        >  2:469008/1‑143 (MQ=255)
                                                                                                    aaCTGGAAGACAGCGCCGCACCGCCATGATTGCGCAGTAACTGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCATCTCTTCGGTACTGACATAACCCTGCTGTTTa                  <  2:613627/143‑1 (MQ=255)
                                                                                                      cTGGAAGGCAGCGCCGCACGGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                <  1:93750/143‑1 (MQ=255)
                                                                                                       tGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCAGCGGTGTAGcc               >  2:289724/1‑143 (MQ=255)
                                                                                                        ggAAGGCAGCGCCGCGCCGGCATGAGGGCGCGGGACGAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGACTAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCa              <  2:163505/143‑1 (MQ=255)
                                                                                                                 cgccgcCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataa     <  1:358132/143‑1 (MQ=255)
                                                                                                                  gccgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCCGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACAGACAAATGCTCTCCCAGCTCTTCGGTACTAACATACACCAGCTGTTTAACCGGTTCGctaat    >  2:471106/1‑143 (MQ=255)
                                                                                                                    cgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAc  >  1:357735/1‑143 (MQ=255)
                                                                                                                                     |                                                                                                                             
AGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC  >  NC_000913/3560323‑3560580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: