Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,876,678	IS5 (+) +4 bp	coding (98‑101/1038 nt)	iap →	aminopeptidase in alkaline phosphatase isozyme conversion

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2288919 =	NA (NA)	32 (0.860)	31/284	0.2	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	2876678 =	0 (0.000)					coding (98/1038 nt)	iap	aminopeptidase in alkaline phosphatase isozyme conversion
*	?	NC_000913	= 2290113	NA (NA)	29 (0.780)	28/284	0.3	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 2876681	0 (0.000)					coding (101/1038 nt)	iap	aminopeptidase in alkaline phosphatase isozyme conversion

TCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2289979‑2290113 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAGCAAAATCGCCAGGTTTAGGTGACGAGGCGTGTACGGGGAGAATAAAGCATACGCCGAGCGCCAGGGCAGCGGTACGGTGGCGCAATGCGGAAAACATAGTGAGTCCTTAAATACCATGCAAATTTTTT  <  NC_000913/2876681‑2876550                                                                                                                                                                                                                                                                             TCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGCTCTATGGCTCCAGATGACAAACATGAACTACTTGCGGGGACGTGGTCGCACCTCCCTCAGCAAc                                                                                                                              >  2:472552/1‑142       TTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGCTGACAAACATGATCTCATATCAGGGACCTGTTCGCACCTTCCTTAGCCAACTCGCC                                                                                                                        >  2:602711/1‑143        TCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAACGTCCATTGCGAGCTCTCTATGAACCCTGTTCTATGTCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCAAAATCGCCA                                                                                                                       <  1:152578/143‑1             TCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTGTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCCAACTCGCCAGGcat                                                                                                                  >  2:440768/1‑140                 ACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGAGACTTCTTCGCACCTTCCTTAGCAAAATCGCCAGGTTCAGGT                                                                                                              >  2:55635/1‑143                   GGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCAAATCGCCAGGTTTAGGTGA                                                                                                            >  1:666519/1‑143                        TTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCCTATCAGGGACTTGTTCGCACCCTCCTTAGCAAAATCGCGAGGTTTAGGTGACGAGG                                                                                                       >  2:57029/1‑143                            TGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCAAAATCGACAGGTATAGGTGACGAGGCGgg                                                                                                   >  2:413452/1‑141                              CTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCAAAATCGCCAGGTGTAGGTGACGAGGCGTGTA                                                                                                 >  1:42198/1‑143                                   TTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGTACTTGTTCGCACCTTCCTTAGCAAAATCGACAAGTTCAGGAGACGAGGCATGTACGGGG                                                                                            >  1:418517/1‑143                                    TCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCAAAATCGCCAGGTTTAGGTGACGAGGCGTGTACGGGGA                                                                                           <  2:121993/143‑1                                             GCGAAGGTAAGTTGATGACTCATGATGCACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCAAAATCGCCAGGTTTAGGTGACGAGGCGTGTACGGGGAGAATAAAGC                                                                                  <  1:546311/143‑1                                                            TGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCAAAATCGCCAGGTTTAGGTGACGAGGCGTGTACGGGGAGAATAAAGCATACGCCGAGCGCCA                                                                   >  1:479013/1‑143                                                            TGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCAAAATCGCCAGGTTTAGGTGACGAGGCGTGGACGGGGAGAATAAAGCATACGCCGAGCGCCA                                                                   >  2:113375/1‑143                                                                TCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATAAGGGACTTGTTCGCACCTTCCTTAGCAAACTCGCCAGGTTTAGGTGACGAGGCGTGTACGGGGAGAATATAGCATACGCCGAGCGCCAGGGC                                                               >  1:7941/1‑143                                                                     tgGAACCCTGTTCTAGTGCTCCCCATGCCACACATGATCTCATATCCGGCACTTTTTCGCACCTTCCTTAGCAAAATCGCCAGGTTCAGGTGACGCGGCGTGTACGGGGAGAATAAAGCATACGCCGAGCGCCAGGGCAGCGG                                                          <  2:184615/141‑1                                                                     ATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCAAAATCGCCAGGTTTAGGTGACGAGGCGTGTACGGGGAGAATAAAGCATACGCCGAGCGCCAGGGCAGCGG                                                          >  2:590270/1‑143                                                                            CTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCAAAATCGCCAGGTTTAGGTGACGAGGCGTGTACGGGGAGAATAAAGCATACGCCGAGCGCCAGGGCAGCGGTACGGTG                                                   <  2:22597/143‑1                                                                                  TATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCAAAATCGCCGGGTTTAGGTGACGAGGCGTGTACGGGGAGAATAAAGCATACGCCGAGCGCCAGGGCAGCGGTACGGTGGCGCAA                                             >  1:608663/1‑143                                                                                   ATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCAAAATCGCCAGGTGTAGGTGACGAGGCGCCTACGGGGAGAATAAAGCATACGCCGAGCGCCGGGGCAGCGGTACGGTGGCGCAAT                                            >  2:308053/1‑143                                                                                                   AACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCAAAATCGCCAGGTTTAGGTGACGAGGCGTGTACGGGGAGAATAAAGCATACGCCGAGCGCCAGGGCAGCGGTCCGGTGGCGCAATGCGGCAGACATGGTGA                            >  1:273410/1‑143                                                                                                       ctATCTCATATCATGGTCTTGTTCGCACTTTCTTTTGCAAAATGGTCAGGTGTAGGTGACGAGGCGTGTACGGGGAGAATAAAGCATACGCCGAGCGCCAGGGCAGCGGTACGGTGGCGCAATGCGGAAAACATAGTGAGTCC                        <  1:502457/141‑1                                                                                                          TCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCAAAATCGCCAGGTTTAGGTGACGAGGCGTGTACGGGGAGAATAAAGCATACGCCGAGCGCCAGGGCAGCGGTACGGTGGCGCAATGCGGAAAACATAGTGAGTCCTTA                     >  1:58223/1‑143                                                                                                            TCATATCAGGGACTTGTTCGCACCTTCCTTAGCAAAATCGCCAGGTTTAGGTGACGAGGCGTGTACGGGGAGAATAAAGCATACGCCGAGCGCCAGGGCAGCGGTACGGTGGCGCAATGCGGAAAACATAGTGAGTCCTTAAA                   >  1:441394/1‑143                                                                                                                 TCAGGTACTTGTTCGCACCTTCCTTAGCAAAAGCGCCAGGTTTAGGTGACGAGGCGTGTACGGGGAGAATAAAGCATACGCCGAGCGCCAGGGCAGCGGTACGGTGGCGCAATGCGGAAAACATAGTGAGTCCTTAAATACCA              <  1:484841/143‑1                                                                                                                       ACTTGTTCGCACCTTCCTTAGCAAAATCGCCAGGTTTAGGTGATGAGGCGTGTACGGGGAGAATAAAGCATACGCCGAGCGCCAGGGCAGCGGTACGGTGGCGCAATGCGGAAAACATAGTGAGCCCTGAAATACCATGCAAA        >  1:639681/1‑143                                                                                                                         TTGTTCGCACCTTCCTTAGCAAAATCGCCAGGTATAGGTGACGAGGCGTGTACGGGGAGAATAAAGCATACACCGAGCGCCAGGGCAGCGGTACGGTGGCGCAATGCGGAAAACATAGAGAGAACTTAAATACCATGCAAATT      >  2:330346/1‑143                                                                                                                            TTCGCACCTTCCTTAGCAAAATCGCCAGGTTTAGGTGACGAGGCGTGTACGGGGAGAATAAAGCATACGCCGCGAGCCAGGGCAGGGGCACGGCGGCACACTGCGGAGAACATAGTGAGCCCGTACATACCATGCAAATTTTT   >  2:399917/1‑143                                                                                                                             TCGCACCTTCCTTAGCAAAATCGCCAGGTTTAGGTGACGAGGCGTGTACGGGGAGAATAAAGCATACGCCGAGCGCCAGGGCAGCGGTACGGTGGCGCAATGCGGAAAACATAGTGAGTCCTTAAATACCATGCAGATTTTTT  >  1:181939/1‑143                                                                                                                                                                                                                                                                             TCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2289979‑2290113 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAGCAAAATCGCCAGGTTTAGGTGACGAGGCGTGTACGGGGAGAATAAAGCATACGCCGAGCGCCAGGGCAGCGGTACGGTGGCGCAATGCGGAAAACATAGTGAGTCCTTAAATACCATGCAAATTTTTT  <  NC_000913/2876681‑2876550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.