Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (‑2/+1)	glpR ← / ← glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	98.4%	203.0 / ‑3.4	64	intergenic (‑2/+1)	glpR/glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (29/34);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑C‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGAC  >  NC_000913/3560316‑3560589                                                                                                                                             |                                                                                                                                        gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcgca                                                                                                                                       <  1:1018636/143‑4 (MQ=255) gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATAAGCACGTTTTCTTCGGTCCATTAAGTCCTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCGGCGCCGCCCCGCCCTGATGGcgca                                                                                                                                       >  2:466201/1‑140 (MQ=255) gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCCGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGACGGCAGCGCCGCACCGCCATGATGGcgcc                                                                                                                                       >  2:780899/1‑140 (MQ=255) gATAAACAGCGTCGAGCCATTGGGGATTTGCTCAGCCACTTTGCGGGCGATGCGCTCTTTTTCTTGGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcaca                                                                                                                                       >  2:288434/1‑140 (MQ=255)     aaCACCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGAt                                                                                                                                  <  1:1126058/143‑1 (MQ=255)      aCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATc                                                                                                                                 <  1:1164520/143‑1 (MQ=255)       cAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGGAGGCAGCGCCGCCCCGCCATGATGGCGA‑AGGATCa                                                                                                                                >  1:128936/1‑143 (MQ=255)        cgCGTCGAGCCATGGGGGATGTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAg                                                                                                                               <  1:395659/142‑1 (MQ=255)         gCGTCGAGCCATTGGGGATGTGCTCCGCCACTTTGCGGGCGATGCGCTCTGTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAgg                                                                                                                              <  1:864564/143‑1 (MQ=255)           gTCGAGCCATTGGGGATTTGCTCCGCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGCTAACCGAACTGGAGGCCCGCGCCCCAGCGCCCTGATGGC‑CAAGGCTCAGGtt                                                                                                                            >  1:426535/1‑143 (MQ=255)            tCGAGCCGTTGGGGTTTTGCTCCGCCACGTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGttt                                                                                                                           <  2:830046/143‑1 (MQ=255)            tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGttt                                                                                                                           <  1:744274/143‑1 (MQ=255)            tCGAGCCAGTGGGGATGTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGttt                                                                                                                           <  1:1209109/143‑1 (MQ=255)              gAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTg                                                                                                                         <  1:965446/143‑1 (MQ=255)                    ttGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTccac                                                                                                                   <  1:141408/143‑3 (MQ=255)                    tcggggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGc                                                                                                                   <  1:131717/141‑1 (MQ=255)                       gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAg                                                                                                                >  2:572260/1‑143 (MQ=255)                           tttGCTCCGCCACTTTGCGGGCGATCCGCTCTTTTTCTTCGGTCTGGCTGACCTGGCGATCGTGCCACGGCGTGTTAACCGAACTGGGAGGCAGCCCCGCACGTCCCTGATGGCGC‑AGGATCAGGGTTTGCTCCGCCCGCTCa                                                                                                            >  1:490100/1‑143 (MQ=255)                              gCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGGAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTg                                                                                                         >  1:359597/1‑143 (MQ=255)                                tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGCTGGCCTTCCGATCGTGCCACGGCGTGTTAACCGAACCGGAACGCAGCGCCGCACCGCCATGATGCCGC‑ACGATCACGTTTTGCTCCGCCAGCCCaccccc                                                                                                       >  2:239517/1‑138 (MQ=255)                                      aCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCGATGCTGGCGC‑AGGATCAGGATCTGCTCCGCCAGCCGATTGAGGTcgcg                                                                                                 >  2:76655/1‑143 (MQ=255)                                                  aTGCGCTCGTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTg                                                                                     <  1:1148483/143‑1 (MQ=255)                                                    gcgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCg                                                                                   <  1:1266826/143‑1 (MQ=255)                                                    gcgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAGAGGCTGCg                                                                                   >  1:1213628/1‑143 (MQ=255)                                                    gcgcTCTTTTTCTTCGGTCTGGGCGGGCTTGCGATCGTGCCACGGCGTGTTGACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCg                                                                                   <  1:1240139/143‑1 (MQ=255)                                                     cgcTCTCTCTCGTCGTGCGGGGCGGCCTTGCGAGCGTGCCACGGCGTGTTAACCGAGCTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTGTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg                                                                                  <  2:1122582/143‑1 (MQ=255)                                                      gcTCCTTTGCTTCGGGCGGGGTGGCCTGGCGAGCGTGCCACGGCGTGTTAGCCGAACTGGACGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCggg                                                                                 <  2:195972/143‑1 (MQ=255)                                                       ttctTTTTCCTCGCTGTGGGTGGCCTTGCGATCGTGCCACGGCGTGGTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGc                                                                                <  1:1045527/142‑1 (MQ=255)                                                       ctctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGc                                                                                >  1:1132328/1‑143 (MQ=255)                                                         ctTTTTCTTCTCTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTg                                                                              <  1:270249/143‑1 (MQ=255)                                                          tttttCTTCGATCTGGCTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGa                                                                             <  1:371322/143‑1 (MQ=255)                                                              tcttcGGTCTGGGTGGCCTTGCGAGCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGga                                                                         <  1:276804/143‑1 (MQ=255)                                                                 gcGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAGCTGGGAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaa                                                                      <  2:694493/142‑1 (MQ=255)                                                                    gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATg                                                                   >  1:1268465/1‑143 (MQ=255)                                                                       tGGGTGGCGTTGCGATCGTGCCACGGCGTTGTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctc                                                                <  2:557871/143‑1 (MQ=255)                                                                         ggcggCCTGGCGGGCGTGCCACGGGGTGTTAACCGAGCTGGAAGGCAGCGCCGCACCGCCACGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTa                                                              <  2:100549/140‑1 (MQ=255)                                                                         ggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTa                                                              >  1:368902/1‑143 (MQ=255)                                                                          gTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCCTTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAc                                                             <  1:1259176/143‑1 (MQ=255)                                                                          gTGGCCTTGCGATCGTGCCACGGCGTGTGAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCCGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAc                                                             <  1:853434/143‑1 (MQ=255)                                                                                  gCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGATCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                     <  2:661151/143‑1 (MQ=255)                                                                                   cGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                                    <  1:572260/143‑1 (MQ=255)                                                                                    gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTCGCGTGCCGTCGAATAGTCTGCGGGCTGACGCAGAAATGCTCTACCAGCTCTTCg                                                   >  2:276913/1‑143 (MQ=255)                                                                                    gATCGTGCCACGGCGTCTTGACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg                                                   <  2:1042907/143‑1 (MQ=255)                                                                                     aTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGCGAACTGCTCTACCAGCTCTTCgg                                                  >  1:322120/1‑143 (MQ=255)                                                                                     aTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCgg                                                  >  1:523762/1‑143 (MQ=255)                                                                                       cGTGCCGCGGCGTGTTCACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                                <  2:954036/143‑1 (MQ=255)                                                                                        gTGCCACGGCGTCTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                               <  1:916681/143‑1 (MQ=255)                                                                                          gCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTTCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTg                                             <  1:322601/143‑1 (MQ=255)                                                                                                    gtTCACCGAGCCGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                   <  1:811522/143‑1 (MQ=255)                                                                                                    gtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCCGCTCTTCGGTGCTGACATAACCct                                   >  2:391234/1‑143 (MQ=255)                                                                                                     ttAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctg                                  <  2:955106/143‑1 (MQ=255)                                                                                                     ttAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctg                                  >  1:410751/1‑143 (MQ=255)                                                                                                      tAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGATTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGGAAGGCTCGACCAGCTCTTCGGTGCTGACATAACCctgc                                 >  2:918265/1‑143 (MQ=255)                                                                                                         ccGAACCTGCAGGCAGTGCCGCACCGCCAGGATGGCGC‑AGGATCATGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCTGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGt                              <  1:466201/143‑1 (MQ=255)                                                                                                          cGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGtt                             >  1:405426/1‑143 (MQ=255)                                                                                                            aaCTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCGCATTGAGGTCGCGGCGAATAGTCTGCGCGCTGACGGAGACAGGCTCTACCCGCTACTCGGTACTGACATAACActgacgcgta                           >  2:1311687/1‑136 (MQ=255)                                                                                                              cTGGAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCCGTTTAAc                         >  2:733926/1‑143 (MQ=255)                                                                                                                 gAAGGCAGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg                      >  1:1146065/1‑143 (MQ=255)                                                                                                                      cAGCGCCGCACCGCCATGCTGGCGC‑AGGGTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGa                 <  2:1180408/143‑1 (MQ=255)                                                                                                                       aGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTCCTGACATAACCCTGCTGTTTAACCAGTTCGat                >  1:972415/1‑143 (MQ=255)                                                                                                                       aGCGCCGCACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGCAGAAATGCTCTACCACCTCTTCGGTACTGACATAACCCTGCTGCTTAACCAGTTCgct                >  2:1221805/1‑141 (MQ=255)                                                                                                                          gccgcACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGttagctact             >  2:618061/1‑136 (MQ=255)                                                                                                                            cgcACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGACTAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTCACATAACCCTGCTGTTTACCCCGGTCGATAATAc           >  2:81054/1‑143 (MQ=255)                                                                                                                             gcACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAcc          >  1:778871/1‑143 (MQ=255)                                                                                                                              cACCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCg         >  1:763947/1‑143 (MQ=255)                                                                                                                               aCCGCCATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGAAATGCTCTACCAGCTCTTCGGTACTGCCATAACCCTGCTGCTTACCCAGTTCGCTAGTACCGt        >  2:224640/1‑143 (MQ=255)                                                                                                                                   ccATGATGGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCgtggtg    >  1:885357/1‑143 (MQ=255)                                                                                                                                     ntgatgGCGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTAGCGGCGAATAGTCTGCGGGCTGCCGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGCTTAACCAGTTCGGTAATAGCGTTGTGAc  >  1:186163/2‑143 (MQ=255)                                                                                                                                             |                                                                                                                                        GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑C‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGAC  >  NC_000913/3560316‑3560589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑