Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 761,422 C→T A906V (GCC→GTC)  sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913761,4220CT98.1% 150.8 / NA 52A906V (GCC→GTC) sucA2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (23/28);  total (23/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGCAGCAGTTTGCTCACGTCAAGGATTTTGTCTGGTGCCAGGAAGAGCCGCTCAACCAGGGCGCATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGCCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTCGAATAAATAAAGGATACACAATGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCT  >  NC_000913/761283‑761557
                                                                                                                                           |                                                                                                                                       
ttGCAGCAGTTTGCTCACGTCAAGGATTTTGTCTGGTGCCAGGAAGAGCCGCTCAACCAGGGCGCATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGtctc                                                                                                                                      >  1:807205/1‑143 (MQ=255)
ttGCAGCAGTTTGCTCACGTCAAGGATTTTGTCTGGTGCCAGGAAGAGCCGCTCAACCAGGGCGCATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGtctc                                                                                                                                      >  1:265719/1‑143 (MQ=255)
   cagcagTTTGCTCACGTCAAGGATTTTGTCTGGTGCCAGGAAGAGCCGCTCAACCAGGGCGCATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTgcgc                                                                                                                                   >  1:393833/1‑143 (MQ=255)
   cagcagTGTGCTCCGGTCAAGGATTTTGTCTGGTGCCAGGCAGGGCCGCTCAGCCAGGGCGCATGGTGCTGCAGCCGGCATCATGTCCGGGAGGGGATTCCGTTTGGGGCTTCTCTGCGTTTTGCAGGCCGCCCGGTCTCCGc                                                                                                                                   <  2:835509/143‑1 (MQ=255)
    agcagTTTGCTCACGTCAAGGATTTTGTCTGGTGCCAGGAAGAGCCGCTCAACCAGGGCACATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCGGcc                                                                                                                                  >  2:480301/1‑143 (MQ=255)
            gCTCACGTCAAGGATTTTGTCTGGTGCCAGGAAGAGCCGCTCAACCAGGGCGCATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCcggc                                                                                                                          <  1:525629/143‑1 (MQ=255)
                 cGTCAAGGATGTTGTCTGGTGCCAGGAAGAGCCGCTCAACCAGGGCGCATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAg                                                                                                                     <  2:361959/143‑1 (MQ=255)
                  gTCAAGGATTTTGTCTGGTCCCCGGCCGCGCCCCCCAACCAGGTGGCGTGGGGCTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAgg                                                                                                                    <  1:353677/143‑1 (MQ=255)
                      aGGATTGTGTCTGGGGCCAGGAAGAGCCGCTCAACCAGGGCGCATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGtat                                                                                                                <  1:613847/143‑1 (MQ=255)
                      aGGAGTTTGTCTGGTGGCAGGAAGAGCCGCTCAACCAGGGCGCATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGtat                                                                                                                <  1:156767/143‑1 (MQ=255)
                       ggATTTTGTCTGGTGCCAGGAAGAGCCGCTCAACCAGGGCGCATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCGGCGACTCTCCAGCGCTAGGGtata                                                                                                               >  1:604951/1‑143 (MQ=255)
                              gTCTGGTGCCAGGAAGAGCCGCTCAACCAGGGCGCATGGTACTGCAGCCAGCATCATTTCCGTGAGGAGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGt                                                                                                        <  1:487410/143‑1 (MQ=255)
                                  ggTGCCAGGAAGAGCCGCTCAACCAGGGCGCATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGCAGGGTATATGTCCGTTCAc                                                                                                    >  1:245736/1‑143 (MQ=255)
                                   gTGCCAGGAAGAGCCGCTCAACCAGGGCGCATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCAcc                                                                                                   >  2:578949/1‑143 (MQ=255)
                                   gTGCCAGGAAGAGCCGCTCAACCAGGGCGCATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCAGGCGGTAGGGTATATGGCCGTTCAcc                                                                                                   >  1:253659/1‑143 (MQ=255)
                                    tGCCATGAAGAGCCGCTCAACCAGGGCGCATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCa                                                                                                  <  1:937061/143‑1 (MQ=255)
                                    tGCCAGGAAGAGCCGCTCAACCAGGGCGCATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCa                                                                                                  <  1:262012/143‑1 (MQ=255)
                                    tGCCAGGAAGAGCCGCTCAACCAGGGCGCATGGTACTGCAGACAGCATCATTTACGTGAAGTGATTCCTTTTGGGGCTTCTCTGCGTTATGCAGCCCGCCCGGTCTCCGCCTATCCGGCGGTAGGGTAGATGACCGATCAccg                                                                                                  >  2:112420/1‑142 (MQ=255)
                                     gCCAGGAAGAGCCGCTCAACCAGGGCGCATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAg                                                                                                 >  2:485429/1‑143 (MQ=255)
                                      ccAGGAAGGGCCGCTCAACCAGGGCGCAGGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGa                                                                                                <  1:187466/143‑1 (MQ=255)
                                        aGGAAGAGCCGCTCAACCAGGGCGCATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGaaa                                                                                              <  1:511013/143‑1 (MQ=255)
                                        aGGAAGAGCCGCTCAACCAGGGCGCATGGTACTCCAGCCAGCATCATTTCCGTGAAGTGATTCGGTATGGGGCGTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCCCTCAGACGGGCGGGTATATGTCCGGTGAccgggaa                                                                                              >  1:358779/1‑138 (MQ=255)
                                          gAAGAGCCGCTCAACCAGGGCGCATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACa                                                                                            <  1:123443/143‑1 (MQ=255)
                                                        cctgggCGCATGGTCCTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTgg                                                                              <  1:8186/140‑1 (MQ=255)
                                                        ccggggCGCATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTgg                                                                              <  2:648806/140‑1 (MQ=255)
                                                             gcgcCTGGTACTGCAGCCAGCATCATTCCCGTGAAGTGCTTCAGTTTGGCCCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAAt                                                                         <  2:457311/143‑1 (MQ=255)
                                                             gcgcATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTCTACGTCCGTTCACCAGCAACAGCAACCAGCTCCGGTTCAt                                                                         >  2:434649/1‑143 (MQ=255)
                                                              cggctGGTACTGCAGCCAGCATCATTTCCGTGGAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATg                                                                        <  2:92185/139‑1 (MQ=255)
                                                                        tGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCGGGTTAATGACGCGCTgga                                                              >  1:430747/1‑141 (MQ=255)
                                                                        ggCAGCCAGCATCTTTGCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGaa                                                              <  1:240507/142‑1 (MQ=255)
                                                                          cagccagcATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATCTGTCCGTTCCCCCGACACAGCAACAGGATCTGGTTAATGACGCGCTGCACg                                                            >  2:489936/1‑143 (MQ=255)
                                                                           agccagcATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCACCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGt                                                           <  2:720172/143‑1 (MQ=255)
                                                                            gccagcATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTc                                                          <  1:483737/143‑1 (MQ=255)
                                                                                 catcatTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCCGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTCGaata                                                     >  2:459933/1‑143 (MQ=255)
                                                                                 catcatTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTCGaata                                                     >  2:454707/1‑143 (MQ=255)
                                                                                 catcatTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTCGaata                                                     >  2:24541/1‑143 (MQ=255)
                                                                                    caattCCGTGAAGGGAGTCCGTCTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTCGaataaat                                                  <  2:953295/140‑1 (MQ=255)
                                                                                     ggttCGGTGGAGTGATTGCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGTGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTCGaataaata                                                 <  2:501058/141‑1 (MQ=255)
                                                                                      tttCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGGTAATGACGCGCTGCACGTCGAacaaataa                                                >  2:88166/1‑143 (MQ=255)
                                                                                         ccGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTCGAATAAATAAAgg                                             <  1:651760/143‑1 (MQ=255)
                                                                                              aaGTGGTTCCGTGTGGGGCTTCTGTGCGTTATGCGGGCCGCCCGGTCTCCGCCGCTCCGGCGGTAGGGTATATGTGCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTCGAATAAATAAAGGATaca                                        <  2:951540/143‑1 (MQ=255)
                                                                                              aaGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGACCGGTCTCCGCCTCGCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTCGAATAAATAAAGGATaca                                        >  1:491877/1‑143 (MQ=255)
                                                                                                    ttCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTCGAATAAATAAAGGATACACAATGa                                  >  2:552307/1‑143 (MQ=255)
                                                                                                         tttGGGGCTTCTCTGCGTTATGAAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACAAGAAACAGCAACAAGCTCTGGTTAATGACGCGCTGAACGTCGAATAAATAAAGGATACAAAATCAGTAGc                             >  2:425557/1‑143 (MQ=255)
                                                                                                               gCTTCTCTGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAGCAGCACCAAGATCTGGTTAATGACGCGCTGAACGTCGAACAAATAAAGGATACACAATGCGTCGCGTAGat                       >  2:25340/1‑143 (MQ=255)
                                                                                                                    tctGCGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTCGAATAAATAAAAGATACACAATGAGAAGCGTAGATATACt                  >  2:476868/1‑143 (MQ=255)
                                                                                                                        cGTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTAGATGTCCGGTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTCGAATAAATAACGGATACACCATGAGCAGCGAAGATATTCAggac              >  2:953812/1‑141 (MQ=255)
                                                                                                                         gTTATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTCGAATAAATAAAGGATACACAATGAGTAGCGTAGATATTCTGGTcc             <  2:265719/143‑1 (MQ=255)
                                                                                                                          ttgtGCAGGCCGCCCGCTCTCCGCCTCGCCTGCGGTAGGGTATCTGTTCGTTCACCAGAAACAGCAACAAGTTCTGGTTAATGACGCGCTGAACGTCGAATAAATAAAGGATACACAATGAGTAGCGTAGATATTCTGGTccc            <  2:124395/140‑1 (MQ=255)
                                                                                                                           tATGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTCGAATAAATAAAGGATACACAATGAGTAGCGTAGATATTCTGGTCCCt           <  1:24541/143‑1 (MQ=255)
                                                                                                                            aTGCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTCGAATAAATAAAGGATACACAATGAGTAGCGTAGATATTCTGGTCCCTg          <  1:449089/143‑1 (MQ=255)
                                                                                                                              gCAGGCCGCCCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTCGAATAAATAAAGGATACACAATGAGTAGCGTAGATATTCTGGTCCCTGAc        <  1:643480/143‑1 (MQ=255)
                                                                                                                                  gccgccCGGTCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTCGAATAAATAAAGGATACACAATGAGTAGCGTAGATATTCTGGTCCATGCcctgc    >  2:440236/1‑143 (MQ=255)
                                                                                                                                    cgccCGGCCTCCGCCGCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTCGAATAAATAAAGGATACACAATGAGTAGCGTAGATATTCTGGTCCCTGAcctgcct  <  2:450135/143‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
TTGCAGCAGTTTGCTCACGTCAAGGATTTTGTCTGGTGCCAGGAAGAGCCGCTCAACCAGGGCGCATGGTACTGCAGCCAGCATCATTTCCGTGAAGTGATTCCGTTTGGGGCTTCTCTGCGTTATGCAGGCCGCCCGGCCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTCGAATAAATAAAGGATACACAATGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCT  >  NC_000913/761283‑761557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑