New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3224522 =46 (1.250)3 (0.080) 3/276 5.3 6.4% coding (1890/3093 nt) ebgA evolved beta‑D‑galactosidase, alpha subunit
?NC_000913 3224536 = 43 (1.200)coding (1904/3093 nt) ebgA evolved beta‑D‑galactosidase, alpha subunit
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GTCACCGTAATGTTGAGGAACGCTTCGCGGGCGTCCAGCTGCGGCAGCGTGATCTGCAAGGGGGCTTCGCTGTTCGGCGCAACGTCGCGCAGTTTAATCTGCTGCGTCGCGAGCGTTTCACCTTCGGCGCGCACCTCTGCGTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3224665‑3224522
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtgcGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCC  >  NC_000913/3224536‑3224675
                                                                                                                                                                                                                                                                                            
GTCACCGTAATGTTGAGGAACGCTTCGCGGGCGTCCAGCTGCGGCAGCGTGATCTGCAAGGGGGCTTCGCTGTTCGGCGCAACGTCGCGCAGTTTAATCTGCTGCGTCGCGAGCGTTTCACCTTCGGCGCGCACCTCTGCGTG                                                                                                                                               <  2:550867/143‑1
     CGTAATGTTGAGGAACGCTTCGCGGGCGTCCAGCTGCGGCAGCGTGATCTGCAAGGGGGCTTCGCTGTTCGGCGCAACGTCGCGCAGTTTAATCTGCTGCGTCGCGCGCGTTTCACCTTCGGCGCGCACCTCTGCGTGCGggg                                                                                                                                          >  1:453768/1‑140
     CGTAATGTTGAGGAACGCTTCGCGGGCGTCCAGCTGCGGCAGCGTGATCTGCAAGGGGGCTTCGCTGTTCGGCGCAACGTCGCGCAGTTTAATCTGCTGCGTCGCGAGCGTTTCACCTTCGGCGCGCACCTCTGCGgggaggg                                                                                                                                          >  1:17241/1‑136
                                                                                                                             GGCGCGCACCTCTGCGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAA                                                                                                        >  1:514459/1‑57
                                                                                                                             GGCGCGCACCTCTGCGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAA                                                                                                        <  2:514459/57‑1
                                                                                                                                             TGCGCGTCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCC  <  2:597094/143‑1
                                                                                                                                                                                                                                                                                            
GTCACCGTAATGTTGAGGAACGCTTCGCGGGCGTCCAGCTGCGGCAGCGTGATCTGCAAGGGGGCTTCGCTGTTCGGCGCAACGTCGCGCAGTTTAATCTGCTGCGTCGCGAGCGTTTCACCTTCGGCGCGCACCTCTGCGTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3224665‑3224522
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtgcGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCC  >  NC_000913/3224536‑3224675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.