Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (‑2/+1)	glpR ← / ← glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	94.4%	297.1 / 6.0	89	intergenic (‑2/+1)	glpR/glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  ref base . (0/5);  new base G (48/36);  total (48/41)
Fisher's exact test for biased strand distribution p-value = 1.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑C‑C‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAA  >  NC_000913/3560183‑3560728                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                    caacggcggGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGGGATGGTTGGTGACAAGGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑cg                                                                                                                                                                                                                                                                                     <  2:445865/271‑2 (MQ=255) cACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCCTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑cg                                                                                                                                                                                                                                                                                     >  1:628768/1‑273 (MQ=255)                tgCGAAAATCTCCTTGTACCATCAACTGGTTGGCAACGTGGGGATTGTTGGTGAAAATGCGCAAATTGCGGTGATGGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGc                                                                                                                                                                                                                                                                    <  2:335/275‑1 (MQ=255)                    aaaaTCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCTGTCTGGGTGGCCTTGCGATCGTGCAACGGCGTGT‑TACCCGAACTGGAAGGCAGCGCCCAACC‑GCCATGCT‑GG‑CGC‑CAGTTCAGGTTTTGCTCCg                                                                                                                                                                                                                                                                >  1:470531/1‑275 (MQ=255)                        gtgtcttTTACCATCAACGTGGTAGCAAGGGTGAGATGGGTGGTGACAAGGGGGAAATTGCTGTGATTGAGCAGGGCGGGCGCTACCGGTGCCGGCGTGGTGCCGATATCGATAAACAGCGTCGGGCCATTGGGGATTTGCTCCGCCACGTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAg                                                                                                                                                                                                                                                            <  2:592152/272‑1 (MQ=255)                           tcttTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTc                                                                                                                                                                                                                                                                  >  1:334419/1‑266 (MQ=255)                               ttACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGcc                                                                                                                                                                                                                                                              <  2:334418/266‑1 (MQ=255)                                    aTCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑CGGATCAGGTTTTGCTCCGCCAGCTCATTGAg                                                                                                                                                                                                                                                   >  1:645017/1‑272 (MQ=255)                                          gtgtTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑cg                                                                                                                                                                                                                                                                                     <  1:274376/233‑2 (MQ=255)                                               aGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCCGCTCATTGAGGTCGCGGCGAATAg                                                                                                                                                                                                                                     >  1:817618/1‑275 (MQ=255)                                                    cGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGCAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc                                                                                                                                                                                                                                >  1:301332/1‑275 (MQ=255)                                                    cGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc                                                                                                                                                                                                                                >  1:260546/1‑275 (MQ=255)                                                     gTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TACCCGAACTGGCAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑CGGACCAGGTTTTGCTCCGCCAGCTCATTGAGGTGGCGGCGAATAGTCTCCg                                                                                                                                                                                                                               >  2:585945/1‑275 (MQ=255)                                                      ttGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg                                                                                                                                                                                                                              >  1:43526/1‑275 (MQ=255)                                                       tGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGAGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCCCATTGAGGTCGCGGCGAATAGTCTGCggg                                                                                                                                                                                                                             >  1:683019/1‑275 (MQ=255)                                                              gttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTTGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTCGCGATCGTGCCCCGGCGTGT‑TAACCGAACTGGAAGGACGCGCCGCACC‑GCCATGCT‑GG‑CGC‑CGGACCAGGTTTTGCTCCCCCAGCCCATTGCGGTCGCGGCGAATAGTGCGCGGGacgacg                                                                                                                                                                                                                       >  2:243982/1‑274 (MQ=255)                                                              gttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGCGT‑TAAACGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGCTCAGGTTGTGCACCGCCCGCCCATTGAGGTCGCGACGAATAGTCCGCCGGCTGACgg                                                                                                                                                                                                                      >  2:282121/1‑275 (MQ=255)                                                                 ggTGACAATGCGCAAATTGCTGTGATTGCGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGAAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGGTTTGCTACGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGGCgggg                                                                                                                                                                                                                    >  1:718345/1‑272 (MQ=255)                                                                  gTGACTATTCTCAAATTTCTGTTATTGAGCAGTGCGGGCGCTACCGCTGCCGGCGTGGTGCCGACAGCGATAAACAGCGTCGATCCAGTGGGGATTTGCTCCGCCACTTGGCGGGCGATGCGCT‑CTTGTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAATCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCGGCTGGCTGACGgaga                                                                                                                                                                                                                   <  1:243982/274‑1 (MQ=255)                                                                      cAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCGGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTGTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGc                                                                                                                                                                                                              <  1:869246/275‑1 (MQ=255)                                                                         tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctct                                                                                                                                                                                                           >  1:789228/1‑275 (MQ=255)                                                                            gcAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCCTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc                                                                                                                                                                                                        <  2:628767/275‑1 (MQ=255)                                                                                   gcggtgATTGAGCAGTGCGGGCGCTACCGCTTCCGGCGGTGTGCCGATATCGATAAACAGCGTCGCGCCATTGGGGATTTGCTCGGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGGCGGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑GAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                                                                                 <  2:69491/272‑1 (MQ=255)                                                                                    cTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                                                                                 <  2:260546/274‑1 (MQ=255)                                                                                    cTGTGAGTGAGCAGTGCGGGCGCTCTCGCTGCCTGGGTGGGGCCGGTATCGATAAACAGGGTCGGGCCATTGGGGATTGGCCCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                                                                                 <  2:658629/274‑1 (MQ=255)                                                                                     tgtgATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAAATGCTCTACCATCTCTTc                                                                                                                                                                                                >  1:658630/1‑274 (MQ=255)                                                                                      gtgATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg                                                                                                                                                                                               >  2:39024/1‑274 (MQ=255)                                                                                         atgtggcggtgcgggcGCTACCGCTGCGGGCGTGGGGCCGATATCGATAAACAGCGTGGCGCGATTGGGGATTTGCGCCGCCACTGTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGAGCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCGCC‑GCCATGAT‑GG‑CGC‑AGGTTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGTCGGAGAAATGCTCTACCAGCTCTTCGGTa                                                                                                                                                                                            <  2:538634/266‑1 (MQ=255)                                                                                          ttGGGCAGTGCGGGCGCTACCGCTTCCGGCGTGGGGCCGAGATCGATAAACTGCGTCGAGCCATTGGGGATTTGCTCCGCCACTGTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGGGT‑TAACCGAACTGGAAGGCAGCTCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                                                                                                                                                                           <  2:444214/274‑1 (MQ=255)                                                                                          ttGCGCAGTGCGTCGCCTACCGCGTCCGGCGTGGTGCCGTTATCGTTAAACAGCGTCTAGCCATTGGGGATTTGCTCCGCCCCTTTGCGGGCGATGCGCG‑TTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                                                                                                                                                                           <  1:865016/274‑1 (MQ=255)                                                                                          ttGAGCAGTGCGTGCGCTACCGCTTCCGGGGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                                                                                                                                                                           <  1:585946/274‑1 (MQ=255)                                                                                                 gtgcgtgcGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGCTCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCCCCGCACC‑GCCATGAT‑GG‑CGC‑CGGATCAGGTTTTTCCCCGCCAGCCCATTGCGGTACAGGCCAATAATCCGCGGGACGACGGAGCAATGCATCACCCGGTCCTCGGTACTGAcgtc                                                                                                                                                                                    >  2:11028/1‑271 (MQ=255)                                                                                                 gtgcgtgcGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATaa                                                                                                                                                                                   <  2:770323/275‑1 (MQ=255)                                                                                                    cgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAccc                                                                                                                                                                                >  1:32240/1‑275 (MQ=255)                                                                                                       gcgcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGACCTGGAAGGGAGCGCAGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGACCCGCCAGCTCATTGAGGTGGCGTCGACTCGTCTGCGGGCTGCCGGAGATATGCCTTACGCGCCTTTCGGGACTGACCTCCCCctgc                                                                                                                                                                             >  2:227713/1‑275 (MQ=255)                                                                                                            aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGCTCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGAAATGCTCTACCAGCTCTTCGGTACTGACCTAACCCTGCTGttt                                                                                                                                                                        >  1:514683/1‑275 (MQ=255)                                                                                                            aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGTAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCCACTAGTCTGCGGCATGACGGCGAAATGCTCTACCAGCTCTTCGGTACGAACATAACCCTGCTGttt                                                                                                                                                                        >  2:558381/1‑275 (MQ=255)                                                                                                            aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCCTTTCGGTCGCGGCGAATAGGCTGCGGGCTGACGGAGACATGCCCTACCAGCCATTCGGTACTGACATAACCCTGCTGttt                                                                                                                                                                        >  2:828499/1‑275 (MQ=255)                                                                                                            aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGCGACATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGttt                                                                                                                                                                        >  1:308064/1‑275 (MQ=255)                                                                                                            aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCCAATACTCTGCGGGCTGACGGCGAAATGCTCTACCCGCCCTTCGGTACTGACATAAcccatccgttt                                                                                                                                                                        >  2:50793/1‑267 (MQ=255)                                                                                                                   ccGGCGTGGTGCCGCTATAGATAAACAGCGCGGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg                                                                                                                                                                  <  2:43526/274‑1 (MQ=255)                                                                                                                     ggCGTGGTGCCGGAATCGTGCAACGGGGTGCGGCCATTGGGGATTGTCGCGGCCACTTTCGGGGCGATGGTGT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt                                                                                                                                                                <  2:764149/274‑1 (MQ=255)                                                                                                                     ggCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcg                                                                                                                                                                                                                                               >  2:577054/1‑195 (MQ=255)                                                                                                                        gtggtgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGa                                                                                                                                                             <  1:169962/274‑1 (MQ=255)                                                                                                                         tggtgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggc                                                                                                                                                                                                                                           <  1:577055/195‑1 (MQ=255)                                                                                                                               ggtagtctaTAACCCGCGTCGGGCCATGGGGTGTGTTGTCGGCCACTGTGCGGG‑GAGTGGCTCCTTTTCCTGCGGTCTGGGTGGCCTTGCGAGCGTGCCACGG‑GTGTGTAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GGTG‑C‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCg                                                                                                                                                     <  2:472777/267‑1 (MQ=255)                                                                                                                                      gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGGTCGATAATACCGTtgtgg                                                                                                                                               >  1:301751/1‑273 (MQ=255)                                                                                                                                        tAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGGTTAACCAGTTCGATAATACCGTTGTGActt                                                                                                                                            >  2:378488/1‑273 (MQ=255)                                                                                                                                        tAAACAGCGGCGAGCCATTTGGGGTTTGCTCCGCCTCTTTGCTGGCGATGGGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCTTGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACg                                                                                                                                             <  1:227713/274‑1 (MQ=255)                                                                                                                                             aGCGGCGGGCCATGGGGGATTGGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGAGCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgtg                                                                                                                                        <  2:378669/274‑1 (MQ=255)                                                                                                                                               cGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGGCCTACCCCTGCTGTTTAACCAGTCCGATAATACCGTtgggcacgtgtgttt                                                                                                                                     >  2:287446/1‑262 (MQ=255)                                                                                                                                               cGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc                                                                                                                                                                    >  2:762657/1‑244 (MQ=255)                                                                                                                                               cGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAAGGCGACATGCCCTACCACCTATTCGGTACTGTCATAACCCTGCTGTTTTACCAGTTCGATAATACCGTGGTGCCGTGGtgttt                                                                                                                                     >  2:472132/1‑275 (MQ=255)                                                                                                                                                gTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGA‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGAGAATCGTCTGCGGGCTGACGGAGAAATGCCCTACCAGCCCTTCGGTACTGACATAACCCGTCTGTTTAACCAGGTCGATAATACCGTTTTGCCCTTGtgttt                                                                                                                                     >  2:686443/1‑274 (MQ=255)                                                                                                                                                 tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgt                                                                                                                                   <  1:39024/275‑1 (MQ=255)                                                                                                                                                  cGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCGTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgtt                                                                                                                                  <  1:147245/275‑1 (MQ=255)                                                                                                                                                   gAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt                                                                                                                                                                <  1:762658/244‑1 (MQ=255)                                                                                                                                                                 ttGCCCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATccc                                                                                                                  <  1:770814/275‑1 (MQ=255)                                                                                                                                                                   gcgcccccACTTGGCGGGCGATGCGCC‑CGTTTTCTTCGGTCTGGGTGGCCTTGCGATCGGGCCGCGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCt                                                                                                                 <  2:514682/268‑1 (MQ=255)                                                                                                                                                                   gCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACg                                                                                                                                             <  1:223072/247‑1 (MQ=255)                                                                                                                                                                   gCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACg                                                                                                                                             >  2:223072/1‑247 (MQ=255)                                                                                                                                                                                   ggCGATGCGCT‑CTTTTTCTTCGGTCTCGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGCAGGCAGCGCCGCGCC‑GCCACGAT‑GG‑CGC‑ATGATCAGGTTTTGCCTCGCCAGCCCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGACCTCCCAGCTCTTCGGTACTGACATAACCCTGCTGTGTAACCCGTTCGTTAATACATATTTGACGTTGTTGTTGTTTCCATTTA‑AAATCCCTTGAATCAttctcgtttt                                                                                                >  2:480767/1‑275 (MQ=255)                                                                                                                                                                                                        cGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAACTGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaac                                                                            >  2:394258/1‑275 (MQ=255)                                                                                                                                                                                                          gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCggg                                                                                                                                                                                                                             >  2:214718/1‑129 (MQ=255)                                                                                                                                                                                                          gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaaca                                                                           >  2:338897/1‑274 (MQ=255)                                                                                                                                                                                                              gggTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGa                                                                                                                                                                                                                         <  1:214718/129‑1 (MQ=255)                                                                                                                                                                                                               ggTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCGCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc                                                                     <  1:558382/275‑1 (MQ=255)                                                                                                                                                                                                                     ctggCGGTCGTGCCACGGGGGGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                                                               <  2:817617/272‑1 (MQ=255)                                                                                                                                                                                                                         cGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAc                                                           >  1:845152/1‑275 (MQ=255)                                                                                                                                                                                                                             cGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCCTTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAGTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACCAAATCACTCGCTACACCCAc                                                       >  1:391938/1‑275 (MQ=255)                                                                                                                                                                                                                              gTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCCCTTTGGTACTGACATCACCCTGCTGTTTACCCAGTTCGATAATACCCTTGTGACGTTGTGTTTGTTT‑CCATTATAAATCCCTGGGATTATTTTCCTTTTCTCGCATTGGACGAATCAACAAAACCAATCGCTAAACCCCCg                                                      >  2:76227/1‑275 (MQ=255)                                                                                                                                                                                                                                         tgt‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGCT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGACATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGCTTTGACGTTGTGTTTGTTT‑CCTTTATAAATACCTGGAATTATTTTCGTTTTCGCGTCTTGATCGAATCAACAACAGCCATCGCTAAACCCACGGCTAAccagcc                                           >  1:35693/1‑271 (MQ=255)                                                                                                                                                                                                                                         tgt‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGCGAAATGCTCTACCAGCTCTTCGGTACTGACGTAACCCTGCTGTTTAACCAGTTCGATAATCCCGTTGCGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCACATCAATAAAAGCCATAGCTAAACTCACGGCTACCCCgtc                                           >  2:598658/1‑273 (MQ=255)                                                                                                                                                                                                                                         tgt‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAACTGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGc                                           >  1:35028/1‑275 (MQ=255)                                                                                                                                                                                                                                         tgt‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGc                                           <  2:32240/275‑1 (MQ=255)                                                                                                                                                                                                                                          gt‑TAACCGAGCTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAAGCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGc                                           <  2:840496/274‑1 (MQ=255)                                                                                                                                                                                                                                          gt‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGc                                           <  2:308064/274‑1 (MQ=255)                                                                                                                                                                                                                                           tgtAACGGGACTGGAAGGCGGCGCCGCACC‑GCCATGGT‑GG‑CGC‑GGGGTCATGTTTTGGTCCGCCAGCACATTGGGGTCGCGGCGAAGAGTATGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CAGTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGc                                           <  2:718344/272‑1 (MQ=255)                                                                                                                                                                                                                                           t‑tAACCTAACTGGAATGCAGGGCCTCACC‑GCCATGATGGG‑G‑C‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGa                                         <  2:845151/275‑1 (MQ=255)                                                                                                                                                                                                                                           gtaaaccagagtGGACGGCGGCG‑CGCACCGGACAGGAT‑GG‑C‑TAAGGATCAGGGTTTGCGCCGCCAGCTCATTGAGGTCGCGGGGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGc                                           <  2:418807/263‑1 (MQ=255)                                                                                                                                                                                                                                                  gAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCACTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAACCACGGCTAACCCGGCGAtgtgtg                                   >  1:478299/1‑275 (MQ=255)                                                                                                                                                                                                                                                    aCTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CCTTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaa                                                                         >  1:335917/1‑235 (MQ=255)                                                                                                                                                                                                                                                    aCTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGc                                  >  1:794377/1‑274 (MQ=255)                                                                                                                                                                                                                                                        gAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc                                                                     <  2:335916/235‑1 (MQ=255)                                                                                                                                                                                                                                                         gaGGCAGGGCCGCCAC‑GCCATGAT‑GG‑CGC‑AGGAGCAGGTTTTGCCCCGCCAGCTCATTGAGGTCGGGGCGAATAGTCGGCGGGCTGACGGAGAAATTCTCTACCAGCTCTGCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGtt                            <  2:35693/274‑1 (MQ=255)                                                                                                                                                                                                                                                                cgccgcACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATc                     >  2:186408/1‑275 (MQ=255)                                                                                                                                                                                                                                                                  ccgcACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAcc                                               >  1:874069/1‑247 (MQ=255)                                                                                                                                                                                                                                                                  ccgcACC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCg                    >  1:20571/1‑274 (MQ=255)                                                                                                                                                                                                                                                                      aCC‑GCCATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGc                                           <  2:874068/247‑1 (MQ=255)                                                                                                                                                                                                                                                                           ccATGAT‑GG‑CGC‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTACGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTAGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGATAACCCGGCGAGGTGTGCTCCGTTCCCCATCGCCATccaa            >  1:110794/1‑272 (MQ=255)                                                                                                                                                                                                                                                                            cATGATGGG‑G‑C‑AGGATCAGTGTTTGCTCCGCCAGCTCATTGGGGTCGCGGCGAATGGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATCCcaaa          <  2:683018/275‑1 (MQ=255)                                                                                                                                                                                                                                                                                    g‑C‑C‑AGGTTCAGGTTTTGCCCCGCCAGCTTATTGCGGTCGCTGCGATTAGTCTGCGGGCTGACGGGTGAATTCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCaa  <  2:301332/275‑1 (MQ=255)                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                    CACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCT‑CTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCACC‑GCCATGAT‑GG‑C‑C‑AGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT‑CATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAA  >  NC_000913/3560183‑3560728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑