Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,779,348 Δ1 bp 14.8% intergenic (‑47/‑269) ydiP ← / → ydiQ putative DNA‑binding transcriptional regulator/putative electron transfer flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,779,3480T.14.8% 85.9 / 7.8 27intergenic (‑47/‑269)ydiP/ydiQputative DNA‑binding transcriptional regulator/putative electron transfer flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (9/14);  new base . (2/2);  total (11/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGCCTGCCGCGTTCTATCACCACAATGTCATCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCGTGTATTGCGGTTATACAACCGCGTTTAGCGAGAGCTAAACAAGATTATTTACACTGAATGCAAGATTGTACGGTCATGGAATAACTATATTGCCGCAAATTGCACTTTTGTTTTAGCGATCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAAC  >  NC_000913/1779067‑1779630
                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                          
gCAGCCTGCCGCGTTCTATCACCACAATGTCATCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATAc                                                                                                                                                                                                                                                                                     <  1:270415/289‑1 (MQ=255)
  aGCCTGCCGCGTTCTATCACCACAATGTCATCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACg                                                                                                                                                                                                                                                                                    <  1:18861/288‑1 (MQ=255)
     cTGCCGCGTTCTATCACCACAATGTCATCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCgt                                                                                                                                                                                                                                                                                >  2:275839/1‑289 (MQ=255)
         cgcgTTCTCTCACCACAATGTCATCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCGTGTAt                                                                                                                                                                                                                                                                            <  1:16713/289‑1 (MQ=255)
         cgcgTTCTATCACCACAATGTCCTCTGTATGTGCCACATATAACGCAGAATCGACTGTAAACCTTGCAACCCCTTTCTTGACGTAAATCTACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTACATGCAAAACGTGAAAGCCGTGGCTTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGAAAAAACAATATATGCCGGATAAAATACGGCGTGTAt                                                                                                                                                                                                                                                                            <  1:138517/289‑1 (MQ=255)
                       acaATGTCATCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCGTGTATTGCGGTTATACAAc                                                                                                                                                                                                                                                              <  1:149697/289‑1 (MQ=255)
                                     aTGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGAAAAATACGGCGTGTATTGCGGTTATACACCCGCGTTTAGCgaga                                                                                                                                                                                                                                                >  1:2853/1‑288 (MQ=255)
                                     aTGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGAAAAATACGGCGTGTATTGCGGTTATACAACCGCGTTTAGCgaga                                                                                                                                                                                                                                                >  1:136396/1‑288 (MQ=255)
                                               tataACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCGTGTATTGCGGTTATACAACCGCGTTTAGCGAGAGCTAAACAAg                                                                                                                                                                                                                                      <  1:166165/289‑1 (MQ=255)
                                               tataACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCGTGTATTGCGGTTATACAACCGCGTTTAGCGAGAGCTAAACAAg                                                                                                                                                                                                                                      <  1:275839/289‑1 (MQ=255)
                                               tataACGAAGAATCGATGGTAAACCTTGCAAACCCTTTCTTGACGTAAATCAACGCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCGTGTATTGCGGTTATACAACCGCGTTTAGCGAGAGCTAAACAAg                                                                                                                                                                                                                                      <  1:306160/289‑1 (MQ=255)
                                                          aTCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGGGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCGTGTATTGCGGTTATACAACCGCGTTTAGCGAGAGCTAAACAAGATTATTTaca                                                                                                                                                                                                                            <  1:86808/288‑1 (MQ=255)
                                                           tCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCGTGTATTGCGGTTATACAACCGCGTTTAGCGAGAGCTAAACAAGATTATTTACACt                                                                                                                                                                                                                          <  1:169328/289‑1 (MQ=255)
                                                                          ccAACACCTTCCTTAACGGAAATCAACTCGGTTTCGTTTTCATGAACGTGATGTCCGAACGCCCTTTTTGGATCATCGCTAAATGCAAAACGTGAAAGACGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGTTACATGACAACCTCCCTATCCCATGAGCAAGCAAAAACAATAGATGCCGGATAAAATACGGCGTGTATTGCGGTTATACAACCGCGTTTAGCGAGAGCTAAACAAGATTATTTACACTGAATGCAAGATTGTa                                                                                                                                                                                                           <  2:36441/288‑1 (MQ=255)
                                                                                         aCGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCGTGTATTGCGGTTATACAACCGCGTTTAGCGAGAGCTAAACAAGATTATTTACACTGAATGCAAGATTGTACGGTCATGGAATa                                                                                                                                                                                              >  1:200372/1‑287 (MQ=255)
                                                                                              aaTCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCGTGTATTGCGGTTATACAACCGCGTTTAGCGAGAGCTAAACAAGATTATTTACACTGAATGCAAGATTGTACGGTCATGGAATAACTatat                                                                                                                                                                                       >  1:192930/1‑289 (MQ=255)
                                                                                                         tttCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCGTGTATTGCGGTTATACAACCGCGTTTAGCGAGAGCTAAACAAGATTATTTaca                                                                                                                                                                                                                            <  1:299265/241‑1 (MQ=255)
                                                                                                            cATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCGTGTATTGCGGTTATACAACCGCGTTTAGCGAGAGCTAAACAAGATTATTTACACTGAATGCAAGATTGTACGGTCATGGAATAACTATATTGCCGCAAATTGc                                                                                                                                                                          >  2:104673/1‑288 (MQ=255)
                                                                                                                   aTGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACGGGCGACAATAAGAGTTTCACTGGCGTTATCAAAACAGGGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATATAATACGGCGTGTATTGCGGTTACACAACCGCGTTTAGCGAGCGCTAAACAAGCTTATTTACACTGAATGCaa                                                                                                                                                                                                                  >  2:299265/1‑241 (MQ=255)
                                                                                                                                 cGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCGTGTATTGCGGTTATACAACCGCGTTTAGCGAGAGCTAAACAAGATTATTTACACTGAATGCAAGATTGTACGGTCATGGAATAACTATATTGCCGCAAATTGCCCTTTTGTTTTAGCGATCGCAt                                                                                                                                                    >  2:207914/1‑289 (MQ=255)
                                                                                                                                                                        aaaGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCGTGTATTGCGGTTATACAACCGCGTTTAGCGAGAGCTAAACAAGATTATTTACACTGAATGCAAGATTGTACGGTCATGGAATAACTATATTGCCGCAAATTGCACTTTTGTTTTAGCGATCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCt                                                                                                              <  2:192930/288‑1 (MQ=255)
                                                                                                                                                                                                                                cGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCGTGTATTGCGGTTATACAACCGCGTTTAGCGAGAGCTAAACAAGATTATTTACACTGAATGCAAGATTTTACGGTCATGGAATAACTATATTGCCGCAAATTGCACTTTTGTTTTAGCGATCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTc                                                       >  1:295104/1‑287 (MQ=255)
                                                                                                                                                                                                                                                   cccTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCGTGTATTGCGGTTATACAACCGCGTTTAGCGAGAGCTAAACAAGATTATTTACACTGAATGCAAGATTGTACGGTCATGGAATAACTATATTGCCGCAAATTGCACTTTTGTTTTAGCGATCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTAt                                   >  1:61127/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                   agcaaAAACAATATATGCCGGATAAAATACGGCGTGTATTGCGGTTATACAACCGCGTTTAGCGAGAGCTAAACAAGATTATTTACACTGAATGCAAGATTGTACGGTCATGGAATAACTATATTGCCGCAAATTGCACTTTTGTTTTAGCGATCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGaaa                  >  1:55994/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                  tGCCGGAAAAATACGGCGTGTATTGCGGTTATACAACCGCGTTTAGCGAGGGCTAAACAAGATTATTTACACTGAATGCAAGATTGTACGGTCATGGAATAACTATATTGCCGCAAATTGCACTTTTGTTTTAGCGATCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAAc  <  2:136396/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  tGCCGGAAAAATACGGCGTGTATTGCGGTTATACAACCGCGTTTAGCGAGAGCTAAACAAGATTATTTACACTGAATGCAAGATTGTACGGTCATGGAATAACTATATTGCCGCAAATTGCACTTTTGTTTTAGCGATCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAAc  <  2:2853/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   gCCGGATAAAATACGGCGTGTATTGCGGTTATACAACCGCTTTTAGCGAGAGCTAAACAAGATTATTTACACTGAATGCAAGATTGTACGGTCATGGAATAACTATATTGCCGCAAATTGCACTTTTGTTTTAGCGATCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAAc  <  1:104673/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                          
GCAGCCTGCCGCGTTCTATCACCACAATGTCATCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCGTGTATTGCGGTTATACAACCGCGTTTAGCGAGAGCTAAACAAGATTATTTACACTGAATGCAAGATTGTACGGTCATGGAATAACTATATTGCCGCAAATTGCACTTTTGTTTTAGCGATCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAAC  >  NC_000913/1779067‑1779630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: