Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,726,507 Δ1 bp coding (485/600 nt) nemR → transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,726,5070T.100.0% 150.8 / NA 34coding (485/600 nt)nemRtranscriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (17/17);  total (17/17)

TCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGAC  >  NC_000913/1726377‑1726644
                                                                                                                                  |                                                                                                                                         
tCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTCACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCCCtt                                                                                                                              >  1:801333/1‑142 (MQ=255)
 cAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACtgt                                                                                                                             <  2:877624/143‑1 (MQ=255)
   gaagaTATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGCACCGCTGCAACAGGCACAAG‑GCTTTACGCACtctgc                                                                                                                           >  1:141513/1‑142 (MQ=255)
             gcAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCGGTGAGGACCATTGATTAACCTTGCGTGGCGGACCGCGGCAACAGGCAAAAG‑GCTTGACGGACGGGTGCGCGGCGCGa                                                                                                                 >  2:419794/1‑143 (MQ=255)
             gcAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCGTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGa                                                                                                                 >  1:78021/1‑143 (MQ=255)
                         aTAACGGGGCTCGCGGCGTGCTGGCCCTGCTCTCTCAGGCGCTGGAAAAGGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCa                                                                                                     <  2:138969/143‑1 (MQ=255)
                          tAACGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCGGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCaa                                                                                                    <  2:766756/143‑1 (MQ=255)
                                 gCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTGACCTATTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTcg                                                                                             >  1:553330/1‑143 (MQ=255)
                                   tCGCGGCGTGATCGCCCTGCTCTCTCGGGAGAAAAATAATTGGCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTcgcg                                                                                           <  1:830032/143‑1 (MQ=255)
                                         cgTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGtct                                                                                     >  1:227182/1‑141 (MQ=255)
                                            gATCGCCCTGCGCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATCGTTTAACCTTTTGTGGCGCACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGa                                                                                  <  1:187186/143‑1 (MQ=255)
                                              tCGCCCTGCTCTCTCAGGCGCGGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGc                                                                                <  1:232441/143‑1 (MQ=255)
                                                         gctcGGGGGCTGGAAAATGGCCGTGAGAACCAGTGTTGAACCTTTTGTGGCGAACCGCTGCAGCGGGCACAAG‑GCTTGACGCGCTGTGGCGTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAAc                                                                     <  2:631656/142‑1 (MQ=255)
                                                                cgcTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTgg                                                              <  1:710377/143‑1 (MQ=255)
                                                                 gcTGGAAAAGGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCAGATATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGc                                                             <  2:188717/143‑1 (MQ=255)
                                                                   tGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTCGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGccc                                                           >  2:118220/1‑143 (MQ=255)
                                                                    ggAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAGCGCGCTGGcccc                                                          >  1:579151/1‑142 (MQ=255)
                                                                     gAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCAt                                                         <  2:640304/143‑1 (MQ=255)
                                                                      aaaaTGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATg                                                        >  1:513125/1‑143 (MQ=255)
                                                                      aaaaTGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATg                                                        >  1:867967/1‑143 (MQ=255)
                                                                                    aaCCATTGTTTAGCCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACattatt                                          <  1:217381/143‑1 (MQ=255)
                                                                                       cATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCg                                       >  1:580192/1‑143 (MQ=255)
                                                                                            tttAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAGAACATTATTGCAACGcc                                  >  1:568689/1‑143 (MQ=255)
                                                                                              tAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTg                                >  2:616129/1‑143 (MQ=255)
                                                                                                  cTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGt                            >  2:79211/1‑143 (MQ=255)
                                                                                                    gtggtgGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGttt                          <  1:587351/140‑1 (MQ=255)
                                                                                                     ttGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTa                         <  1:29444/143‑1 (MQ=255)
                                                                                                        tgGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTAGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATCATTGCGACGCCTGCCGTTTAGCa                      >  1:516163/1‑143 (MQ=255)
                                                                                                         ggCGCAGCGCTGCAAAACGGACAAG‑GCTTTACGCACTGGGGGTTGGCGCGAAGCTGCAGGCCAACATTTCGCGGAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAg                     <  2:76347/143‑1 (MQ=255)
                                                                                                          gCGAACCGCTGCAACAGGCACACG‑GCTTTTGTTACTGTGTCTTGGCGCGATTTTGCAGGCCTAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAgg                    <  1:235484/143‑1 (MQ=255)
                                                                                                          gCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTACGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCCTGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAgg                    >  2:441235/1‑143 (MQ=255)
                                                                                                                   tGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGACACTGGGGAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTa           <  1:554056/143‑1 (MQ=255)
                                                                                                                     cAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATc         <  2:553330/143‑1 (MQ=255)
                                                                                                                            cacaAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAgac  >  1:689761/1‑143 (MQ=255)
                                                                                                                                  |                                                                                                                                         
TCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGAC  >  NC_000913/1726377‑1726644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑