| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 97.4% | 104.8 / NA | 39 | I204S (ATC→AGC) | wcaA | putative glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (19/19); minor base T (0/1); total (19/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTG > NC_000913/2132650‑2132923 | tCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGGCGAAAGTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc < 1:498920/143‑1 (MQ=255) ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGGGGTCGAATTTGTCGTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTGGCCGTGATTGATATGCAGGCTCTGCGTc > 2:149491/1‑143 (MQ=255) gCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTc > 1:650892/1‑143 (MQ=255) gCTGATATCTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTc < 1:269184/143‑1 (MQ=255) tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa > 1:274820/1‑143 (MQ=255) agtttttttGCTGGCTCGGTCGAATTTGTCTTTGTGCTTGCGTTACGCGTGTCAGTACCCGGCGACTTTTTGCGGTGAGGGGGTGACCTGCATTTCACCGTTATTGTTATGCAGGTTCTGCGTCGCCTACTCTCCTGTCCACg < 2:226749/140‑1 (MQ=255) ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATCTGCAGGCTCTGCGTCGACCCTTCTACTTTCCACgtgt > 1:232851/1‑140 (MQ=255) cTGTCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa < 1:588241/143‑1 (MQ=255) cTGGCGCGGTCGGAGTTGTCTTTGTGCTGGCGGTAGAGGTGGAAGTGCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGGTGTGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa < 2:95912/143‑1 (MQ=255) gcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcca > 1:136093/1‑143 (MQ=255) tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccac > 2:601741/1‑143 (MQ=255) cGACTTTGTGTGTTTGCTTGCGGTAAAAGTGTAAGTCCCCGTTGAATTCTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc < 2:428560/143‑1 (MQ=255) tttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATcc > 2:776480/1‑143 (MQ=255) gTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCa > 1:220049/1‑143 (MQ=255) ttGTGCTTGCTTTATAAGTGGATGTTCCCGGAGTATTTTTTCGGTGTCGAGGTGATCTGCATTGCACCGGGATAGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa < 1:530041/143‑1 (MQ=255) ttGTGCTTGCGGTATATTTGGTATTTTCCGGAGTATTTTTTCGGTTTCGTGGTGATTTGCATTTCACCATGATTGATATGCAGGCTCTGCGGCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa < 1:326323/143‑1 (MQ=255) cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCAGTACTCCAACACCATCCACAGGAAGATGt > 2:865176/1‑143 (MQ=255) gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg > 1:262792/1‑143 (MQ=255) gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg > 1:710279/1‑143 (MQ=255) gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg > 1:717292/1‑143 (MQ=255) agaCTTTCTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg < 1:273152/143‑1 (MQ=255) ttCGGTGACGAGGTGATCTGCATTTCACCGTGAATGATATGCAGGCTCTGCGTCGCCTCTTCTACCTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa < 1:715836/143‑1 (MQ=255) cgcggTCATCTGCATTTCACCGTGATTGATCTGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACAct < 1:315935/140‑1 (MQ=255) ccaGGTGATCTGCATTTCCCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACAct < 1:256952/141‑1 (MQ=255) aGGTGAGCGGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACActct < 1:624718/143‑1 (MQ=255) ggTGATCTGCATTTCACCGTGTTTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCtt < 2:686216/143‑1 (MQ=255) gTGATCTGCCTTGCACCGTGATTGATGTGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCttt < 2:449965/143‑1 (MQ=255) tGCATTTCACCGTGATTGATATGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGAGGGCGTAATGCTGTGCCGCCGTCAGTGCGGAATAGAACAAGCACTCTTTGcaaag > 2:850131/1‑141 (MQ=255) gtgcACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTCTCCACGGTTCGCCGTGCTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCAt < 1:785611/140‑1 (MQ=255) aCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc > 1:843539/1‑143 (MQ=255) cgggattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACCCCACCACCATCGGCAGGAAGAGGTCGTAATCACGTGCCGCTCTCAGTTCGATATCGACCGAACACTCTTTGAAACGCTATGCCAAg > 2:316735/4‑143 (MQ=255) cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCac > 2:596857/1‑142 (MQ=255) cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg > 2:486888/1‑143 (MQ=255) ttgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTCCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa < 2:523797/143‑1 (MQ=255) tgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCTCAGGAAGCTGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAACCCCCATGCCCCGGTAAAg > 1:482095/1‑143 (MQ=255) tgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACACTTTGAAACGCCATGCCCAGGTAAAg > 2:597740/1‑143 (MQ=255) tgacATGCGGGCTCTGCGTCGCCTATTCGAGTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAg < 2:693784/143‑1 (MQ=255) atatGCAGGCTCTGCGTCGCCACTTCTACTTGCCACGGGTCGCCGTACTCCACCGCCGTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAc < 1:326112/143‑1 (MQ=255) tGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTg < 1:601741/143‑1 (MQ=255) | TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTG > NC_000913/2132650‑2132923 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |