Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 153,986 IS5 (–) +4 bp coding (1438‑1441/2598 nt) htrE ← putative outer membrane usher protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 153986 =0 (0.000)52 (1.810) 34/284 0.0 100% coding (1441/2598 nt) htrE putative outer membrane usher protein
?NC_000913 = 275149 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 = 1539890 (0.000)22 (0.760) 19/284 0.5 100% coding (1438/2598 nt) htrE putative outer membrane usher protein
?NC_000913 273955 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region

TTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  >  NC_000913/273823‑274096
                                                                                                                                    |                                                                                                                                             
ttAATGACGGCTTGATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGa                                                                                                                                     <  2:148911/143‑1 (MQ=255)
                cTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATAGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAAttc                                                                                                                     >  1:386166/1‑143 (MQ=255)
                            ccggCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGAc                                                                                                         <  2:20657/141‑1 (MQ=255)
                                   aTTCGCAACGGCCGTCGTGATGGCGAGATGCGAGTGGTCTCAACGAGCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtca                                                                                                  <  2:474976/143‑1 (MQ=38)
                                        cAAACGGCGTCGTTGTGGCGAGATGCGAGCGGCCTCAACGAGCCCGCAGGATAAAGCCAGAAACTTCGCGGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttca                                                                                             <  2:77855/143‑1 (MQ=25)
                                                               tGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAttt                                                                      <  2:440146/143‑1 (MQ=255)
                                                                gCGAGCGGTCTCAACGGTCCCGCAGGAGAAAGCCAGAAACGGCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttt                                                                     <  2:335553/143‑1 (MQ=255)
                                                                   cgTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtc                                                                  <  1:175974/142‑1 (MQ=255)
                                                                    gTGGTCTGAACGTTCCCGCAGGAGAAAGCCAGAGACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtct                                                                 <  2:241165/143‑1 (MQ=255)
                                                                    gTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGCTTGAGCCGAtttttccc                                                                 >  2:508314/1‑140 (MQ=255)
                                                                       gTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCCTCGCTGACAGCACTGTTCTGGAAGGTGCGAATCAGCGGGGAAATTCTTCTCGGCTGACGCAGTCATTTCATTTCTTCATGTTTGAGCCGCTCTTTTCTccc                                                              >  2:264595/1‑143 (MQ=21)
                                                                            aaCGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACCGCCATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTaaa                                                         >  1:410278/1‑143 (MQ=255)
                                                                                          agaAAGCGAGAAGCGTCGCCGCATCGCGGACAGCACTGTTATGGGAGGTGCGGATAAGCGGGGAAATTCTTGTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGc                                           <  2:159456/143‑1 (MQ=17)
                                                                                                ccAGAATCTTCGCCGCATCGCTGTCAGCACTGTTATGGATGGTGCGTATATGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATGTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTAttt                                     <  1:60225/143‑1 (MQ=17)
                                                                                                ccAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGCATCAGCCTAtgt                                     >  2:94616/1‑141 (MQ=255)
                                                                                                    aaaCTGCGCCGCTGCGCAGACCGCACTGTGATGGAAGGTGCGAATAAGCGGGGAAATTCTGCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGGAAATGCCTTGAATCAGCCTATTTAGAc                                 <  1:134992/143‑1 (MQ=14)
                                                                                                         tCGCCGCGTCGCTGACAGCACTGTTATGGAAGGTGCGAATAGGCTGGGAGTTTCTTCTCGGATCACGCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGttt                            <  2:393731/143‑1 (MQ=14)
                                                                                                             cgcATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGCttcttc                        <  1:448442/143‑1 (MQ=255)
                                                                                                                   gCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCCGCCTCAGTTATTTTATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTCCTTCGCCAtt                  >  1:267099/1‑143 (MQ=14)
                                                                                                                      gACAGCACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTaa               <  2:465334/143‑1 (MQ=17)
                                                                                                                       aCAGCACTGTTATGTCAGGTGCGAATAAGCGGGCAAATTCTTCTCGGCTGACTCATTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAg              <  2:20926/143‑1 (MQ=255)
                                                                                                                          gCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCg           <  2:26073/143‑1 (MQ=34)
                                                                                                                              tGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGATCAGGCTAATTAGACCGTTTCTTCGCAAGTTAAGGCGTTAt       >  2:474908/1‑143 (MQ=11)
                                                                                                                                   tGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCa  <  1:361173/143‑1 (MQ=11)
                                                                                                                                    |                                                                                                                                             
TTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  >  NC_000913/273823‑274096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.