| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,158,384 | G→A | W172* (TGG→TGA) | ptsG → | fused glucose‑specific PTS enzymes: IIB component/IIC component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,158,384 | 0 | G | A | 90.0% | 44.0 / ‑5.9 | 21 | W172* (TGG→TGA) | ptsG | fused glucose‑specific PTS enzymes: IIB component/IIC component |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (7/11); minor base C (0/2); total (7/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01 | |||||||||||
CGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGA > NC_000913/1158245‑1158526 | cGATCGCAGCGTACATGTTTGACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGaccg < 2:220708/143‑1 (MQ=255) gATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGCCCTGGCTGCCATCTTTCCTGGCGTTGTGCTGTCCTTCCTTTGcccgc > 2:122162/1‑143 (MQ=255) aTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTCAACGCTTTGTGCCGATCATGTCTGGCCTCGCTGCCATCTTTACTGGCGTTGTGATGTCCTTCATTTGaccccc > 2:212340/1‑143 (MQ=255) gCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTgg < 1:93904/143‑1 (MQ=255) gTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGtt < 1:114965/143‑1 (MQ=255) ccgtGTTTTGCCGTTTCTACCGTGTTACGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGTTCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGCCCGCCGATTGGTTCt < 2:364537/140‑1 (MQ=255) cgtGTTTAGCCGTTTCTACCGGATTAAGCTGCCTGAGTATCGTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGTTCTg < 2:133923/141‑1 (MQ=255) gTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTCCTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGTTCTGCaa < 1:426281/143‑1 (MQ=255) tttAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCAGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTAATTTGACAGCCCATTGGTTCTGCaaa > 2:165748/1‑142 (MQ=255) ttAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGTTCTGCAATc < 1:194135/143‑1 (MQ=255) tACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGTTCTGCAATCCAGACCTtctc > 1:171137/1‑143 (MQ=255) aaGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGgc > 1:66846/1‑143 (MQ=255) gTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGTTCTGCAATCGAGCCGGTCTCTCAGTGGGCTGCTTACCAGa > 1:17129/1‑143 (MQ=255) ggCTGCTTTGCCGGTAAACGCGTTGGGCCGATCAGTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGt < 1:142445/143‑1 (MQ=255) cttcttTGCCGGTAAACGCTTTGTGCCGTTCATTTCTGGCCTGGCTGCCATCATTACTGGCGTTGTGCTGTCCTACATTTGACCGCCGATAGGTCCTGCAATCCAGACCTTCTCGCAGTGGACTACTTACCAGAACCCgaaaa > 2:194426/1‑139 (MQ=255) tcttTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTTGGCTGCTTACCAGAACCCGGTAGtt < 1:136588/143‑1 (MQ=255) ccGGTAAACGCTTTGTGCCGATCATGTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGTCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGttt < 1:219712/143‑1 (MQ=255) cGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTa > 1:98506/1‑143 (MQ=255) gATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCg > 2:441452/1‑143 (MQ=255) ttctttCTGTCTTGGCTGCCATCTTTTTTGGCGTTGTGCTGTCCTTCATTTGACCGCCGTTTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGTACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGaa < 2:411264/140‑1 (MQ=255) ggcctggcGGGCCTCTTTAGCGCTGTGTTGCTGTCCCTCGGTTGACCGCCGATTGGTTCTGCGATCCATGCCTTGTCTCGGTGGGCTGCTTACCAGAAGCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCt < 2:444979/143‑1 (MQ=255) gCGTGGTGCTCTCGTTGCGTTGCCCGCCGATTGGTTCTTCCATCTAGTCCTTCTCTCAGTGTGCTGCTTACCAGACCCCGGCAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCGGGTACCGTTTGGTCTGcaccac < 2:396658/143‑1 (MQ=255) cTTCAGTTGACCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTAc < 1:212340/143‑1 (MQ=255) ttGACCTCCGATTGGTTCTGCCATCCAGACCTTCTCTCAGTTGGCTTCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTcc < 1:143562/143‑1 (MQ=255) gccgccGTTTGGTTCGGCATTCGAGACCTGCGCGCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGa < 2:270526/143‑1 (MQ=255) | CGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGA > NC_000913/1158245‑1158526 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |