| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,157,900 | +A | coding (32/1434 nt) | ptsG → | fused glucose‑specific PTS enzymes: IIB component/IIC component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,157,896 | 1 | . | A | 88.9% | 20.0 / ‑3.0 | 9 | coding (28/1434 nt) | ptsG | fused glucose‑specific PTS enzymes: IIB component/IIC component |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (6/2); total (6/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
ATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGC‑AAA‑AGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCC > NC_000913/1157785‑1158030 | aTGCCCTGTACACGGCCAGGCTCTACCCCCTTGCCACGCGTGAGAACGTAAAAACAGCACCCATACTCACCAGCACTCTCAATTATGTATAAGAATGCATTTCCTAACCTGCAAAA‑AGGTCCGTAAATCGCTAAtgctgcagc > 2:254246/1‑140 (MQ=255) tttACACGGCGAGGCTCTCCCCCCTTTCCACTCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAA‑AGGTCGGTAAATCGCTGATGCTGCCGGTATCCg < 1:78181/141‑1 (MQ=255) gAGGGTCTCCCCCCGTGCCACGCGTGAGAATGTAACAAAAGCACCCATACTCAAGAGCACTCTCAAGTATGTTTAAGAATGCATTTGCTGACCTGCAAAA‑AGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTAt < 2:57874/143‑1 (MQ=255) tataaCGTCAAGAAAGCACCCGTTCTCCGGAGCACTCTCAATTATGCTTAAGAATGCATTTGCTAACCTGC‑AAATAGGTCGGTAAAGCGCTGATGCTGCCGGTATCCGTATTGTCTATCCCAGGTATTCTGCTGGGCGTCGGt < 2:198222/140‑1 (MQ=255) cAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAA‑AGGTCGGTAAATCGCTGATGCTGCCGGTCTCCGTACTGCCTATCGCATGTATTCTGCTGGGAAGCGAGAACGCGAGTTTCAGCTGGCTGCCCGCCGTTGTATCGCa > 1:295206/1‑143 (MQ=255) tAAGAATGCATTTGCTAACCTGCAAAA‑AGGTCGGTAAATCGCTGATGCTGCCTGTATCCGTACTGCCTATCGCAGGTATTCTTCTGTGCGTCGGTTCCACGAATTTCAGCTAGCTGACCGCCGTTGTATCGCATGTTATGTCa > 1:190141/1‑143 (MQ=255) cATTTGCTAACCTGCAAAA‑AGGTCGGTAAATCGCTGATGCTGACGGTATCCGTACTGCCTATCGCAGGTATTCTGCTCGGCGTCGGTTCCGCGAATTTCAGCTCGCTGCCCGACGTGGTATCGCAGGTTCTGGCGGAAGCAgg > 2:8826/1‑143 (MQ=255) tAACCTGCAAAA‑AGGTCGGTACATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCCTTCTATCTCATGTTCTGGCAGAACCAGGCCGTTcc > 2:77488/1‑143 (MQ=255) tAACCTGCAAAA‑AGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATAGCAAAAGCAGGCggaagc > 1:292791/1‑139 (MQ=255) | ATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGC‑AAA‑AGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCC > NC_000913/1157785‑1158030 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |