Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	704,008	C→A	P22Q (CCG→CAG)	nagE →	N‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	704,008	0	C	A	100.0%	34.9 / NA	16	P22Q (CCG→CAG)	nagE	N‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (8/8);  total (8/8)

CGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAAACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAG  >  NC_000913/703870‑704142                                                                                                                                           |                                                                                                                                       ctgCAGTACAATTTGCAGCAAACTAAAAATACGTCTTGAAACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATGGCGGTGCTGCAGGTg                                                                                                                                    <  2:322229/141‑1 (MQ=255)     aGTACAATTTGCAGCAAAATAAAAATACGGCTTGAAACGAGCCAACTAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTAGAGCTCCCTATTGTGGTTCTGCAGGTagcgg                                                                                                                                >  1:56840/1‑143 (MQ=255)         cAATTTGCAGCAAAATAAAAATACGGCTTGAAACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTgggcgaccg                                                                                                                            >  2:297018/1‑136 (MQ=255)             ttGCAGCAAAATAAAAATACGGCTTGAAACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTtg                                                                                                                        <  2:261785/143‑1 (MQ=255)                                cGGTTTGAAACGAGCCAAATATGTTTTTTGTAGTGTGAATAAGATGAATATTTTAGGGTGTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAg                                                                                                     <  1:99923/143‑1 (MQ=255)                                      gtaaCGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGTGCGTTATATTTTCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTAc                                                                                               <  1:16701/141‑1 (MQ=255)                                           gAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTAGGTCAGCCAGATTTACTTAAc                                                                                          >  1:363366/1‑143 (MQ=255)                                              ccAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTCGGGCGTTACAGCTCCCTATCGCGGTACTGCAGGTGGCGACACTGTTGATGCGATTCGGACAGCCAGAATTACTTAACGtt                                                                                       >  1:54102/1‑143 (MQ=255)                                                      ggTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTGCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTAt                                                                               <  1:368319/143‑1 (MQ=255)                                                         tctcGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGc                                                                            >  2:190352/1‑143 (MQ=255)                                                                          gATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGAGTTACTTAACGTTGCGTTTATTGCCCACGCGGGCGGTGCGa                                                           >  2:505/1‑143 (MQ=255)                                                                                tatTTTAGTTTTTTTCTAGCTTCTCTGTAGGGCGTTACAGCCCCCCATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTg                                                     <  1:54084/143‑1 (MQ=255)                                                                                         ttttttCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCtag                                            >  2:275578/1‑141 (MQ=255)                                                                                                    gtcTCGGTAGGGCGTTACAGTTCCCTATTGCGGTGCTGCAGGTGGCGGCATTGTTGTTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTc                                 <  1:266285/141‑1 (MQ=255)                                                                                                                cGTTACAGCGCCCTAGCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTGGGCCAGATTTACTTGACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGtgtg                     <  2:81736/143‑1 (MQ=255)                                                                                                                                   ggTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCCTCGCCATCGGTGTGGCATCCCGCTGGTCGAAAg  >  2:83588/1‑143 (MQ=255)                                                                                                                                           |                                                                                                                                       CGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAAACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAG  >  NC_000913/703870‑704142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑