New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 253246651 (1.260)3 (0.080) 3/272 6.1 5.9% coding (58/972 nt) cysK cysteine synthase A, O‑acetylserine sulfhydrolase A subunit
?NC_000913 = 2532494 47 (1.210)coding (86/972 nt) cysK cysteine synthase A, O‑acetylserine sulfhydrolase A subunit
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTG  >  NC_000913/2532324‑2532603
                                                                                                                                              |                                                                                                                                         
gATATTCTAAATCCTTACTTCCTCATATTCTCTGCGCGTGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGcc                                                                                                                                           <  2:221204/143‑1 (MQ=255)
gATATGCTAGATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGcc                                                                                                                                           <  2:165109/143‑1 (MQ=255)
       tAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCGGTAGAATCCCAATTTCATACAGTTAAGGCCAGGCCATGAGTCAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCg                                                                                                                                    >  1:521753/1‑143 (MQ=255)
       tAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATACTACCCGTTTCTTCCCAATTTCATACAGTTAAGAACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCg                                                                                                                                    >  1:502955/1‑143 (MQ=255)
        aaaTCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGc                                                                                                                                   >  2:159926/1‑143 (MQ=255)
         aaTCCGTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTGACACGCCGCTGGTTCGCCTGAATCGCa                                                                                                                                  <  2:563723/143‑1 (MQ=255)
            ccTTACGTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGTCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCg                                                                                                                               <  2:231870/143‑1 (MQ=255)
              ttACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGCTTCACAATTTCATACAGTTAAGGACAGGCCATGCGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCggc                                                                                                                             >  2:597758/1‑142 (MQ=255)
                aCTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGACTGAATCGCATCGGTaa                                                                                                                           >  2:302557/1‑143 (MQ=255)
                     cGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGAc                                                                                                                      <  2:586823/143‑1 (MQ=255)
                       cATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTCACGGACGc                                                                                                                    >  1:74342/1‑143 (MQ=255)
                             ctctGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCCGTAACGGACGACTTCTg                                                                                                              >  1:109444/1‑143 (MQ=255)
                              tctGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTgg                                                                                                             >  1:638986/1‑143 (MQ=255)
                                 gAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGa                                                                                                          <  1:341575/143‑1 (MQ=255)
                                      ggTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGcccaaggc                                                                                                     >  2:507925/1‑136 (MQ=255)
                                      ggTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTg                                                                                                     <  2:203902/143‑1 (MQ=255)
                                           gCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAAtc                                                                                                >  2:565557/1‑143 (MQ=255)
                                            cTACCTGTTGTATCCCAATTTCATACAGGTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGGTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGGGGAAtct                                                                                               >  1:522623/1‑143 (MQ=255)
                                             tACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTCACGGACGCATTCTGGCGAAGGTGGAAtctc                                                                                              >  1:283464/1‑143 (MQ=255)
                                                  gttgtATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAAc                                                                                         >  1:1045/1‑143 (MQ=255)
                                                  gttgtATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAAc                                                                                         >  1:699023/1‑143 (MQ=255)
                                                        ttccAATTTCATGCAGTTAAGGACAGGCCATGTGTAAGATTTTTGAAGATACCTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGc                                                                                   <  2:533012/141‑1 (MQ=255)
                                                        tCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCCTTCTGGCGAAGGTGGAATCTCGTAACCCCAGc                                                                                   >  1:661850/1‑143 (MQ=255)
                                                        tCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTGCGCCTGAATCGCATCGGTAACGGACGCCTTCTGGAGAAGGTGGAATCGCGTAACCCCCGc                                                                                   >  1:422296/1‑143 (MQ=255)
                                                          ccAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGCAGGTGGAATCTAGTAACCCCAGcct                                                                                 >  2:675360/1‑141 (MQ=255)
                                                          ccAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCtt                                                                                 >  2:194869/1‑143 (MQ=255)
                                                            aaTTTCATACAGTTAGGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCGGCGTCa                                                                               >  2:406448/1‑143 (MQ=255)
                                                              ttccATACAGTTAAGGACAGGCCATGAGTAAGCTTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGc                                                                             <  1:319102/140‑1 (MQ=255)
                                                                 cATGCAGTTAAGGACAGGCAAGGAGCAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGtt                                                                          <  2:513616/143‑1 (MQ=255)
                                                                  acaCATTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATACCTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTa                                                                         <  2:560086/141‑1 (MQ=255)
                                                                        ttAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGTACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGGTAAGTGcc                                                                   >  2:128545/1‑143 (MQ=255)
                                                                        tgaaGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGcc                                                                   <  2:186156/141‑1 (MQ=255)
                                                                         tAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCg                                                                  <  1:159926/143‑1 (MQ=255)
                                                                          caGGACGGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGt                                                                 <  2:66373/142‑1 (MQ=255)
                                                                              ccAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGCCACACGCCGCTGGTTCGCCTGAATCGCATCGGGAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCg                                                             <  2:200650/142‑1 (MQ=255)
                                                                               cAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCgg                                                            <  1:352531/143‑1 (MQ=255)
                                                                                  gCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTACCCCAGCCTCAGCGTTAAGTGCCGTATCGGTGcc                                                        >  2:143231/1‑143 (MQ=255)
                                                                                     ctGAGGAAGATGTTTGAGGGTAACTCGCGGGCGGGCGGTCACACGCCGCTGGTTCGCCGGAGTCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCGCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCaa                                                      <  2:12238/142‑1 (MQ=255)
                                                                                            aGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGagt                                               >  1:107830/1‑141 (MQ=255)
                                                                                              aTTTTGGAGGATAACTCGCTGACTATCTGTCACACGCCGCTGGTTCGGCTGAATCGAGCCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTg                                             <  2:352474/143‑1 (MQ=255)
                                                                                               tttttGACGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCCTCGGTAGCGGTTGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTgg                                            <  1:535033/143‑1 (MQ=255)
                                                                                               tttttGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTgg                                            <  1:433371/143‑1 (MQ=255)
                                                                                                     aaGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGCAGGTGGAATCTCGTAACCCCAGCTTCCGCGTGAAGTGCCGTAACGGTGCCCACACGCATCGGGATGcc                                      >  2:498472/1‑143 (MQ=255)
                                                                                                             tCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAgcg                              >  1:285237/1‑143 (MQ=255)
                                                                                                               gCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGcgcg                            <  2:283464/143‑1 (MQ=255)
                                                                                                                          gTCACGCGTCGTTGGTTCCGCTGAATCGCATAGGGAACGGACGCATCCGGGCGCAGGTGGAATCTCGCAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGaaa                 <  2:152791/143‑1 (MQ=255)
                                                                                                                           tCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAGGCGCGACGTGCTGacgc                >  2:386514/1‑140 (MQ=255)
                                                                                                                               acGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTTGAAACAgg            >  1:267529/1‑143 (MQ=255)
                                                                                                                               acGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAgg            >  2:731486/1‑143 (MQ=255)
                                                                                                                                  ccgcTGGGTGGCCTGACTCGCATCGGTAACGGACGCATTCGGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATGGGTGTCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGt         <  1:519025/143‑1 (MQ=255)
                                                                                                                                   cgcCGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGACAGTGGAAGCTCGTAACCCCAGCTCCAGCGTTAACTGCCGGATCACTGCCAACATCATTGACGACGCCGAAAATCGCGACGTGCAGAAAGCAcgcgct        >  2:118091/1‑141 (MQ=255)
                                                                                                                                        gTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTACGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACt   <  1:597758/143‑1 (MQ=255)
                                                                                                                                         ttCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGGTGAACTg  >  2:597842/1‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                         
GATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTG  >  NC_000913/2532324‑2532603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.