| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,726,169 | T→G | F49L (TTT→TTG) | nemR → | transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,726,169 | 0 | T | G | 97.5% | 116.5 / NA | 40 | F49L (TTT→TTG) | nemR | transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (20/19); minor base C (1/0); total (21/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGA > NC_000913/1726034‑1726305 | cacCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTACCGCACTa > 2:431978/1‑143 (MQ=255) ccGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTaaa < 2:596203/143‑1 (MQ=255) aaCATGATACTCGCGAACATCTCCTGACGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAaagaa < 1:23450/143‑1 (MQ=255) cATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGc < 1:540728/143‑1 (MQ=255) aCATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCCCCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATACCTACTTGCGCCCTAACGAAGCGTTTGGCGTTGCCa > 2:304376/1‑143 (MQ=255) tctcCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCCCTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGc < 1:319361/143‑1 (MQ=255) tcCTGGCGACGGGCGCGCAACTTTGCCTGCCACGTGGACTCACCGGGATGGGGCTAAGCGCATTACGAGAAACCGCCGAAGTGCCGCAAGGGACCCGCTATCACACCTCGACCTCCAAAGCAGCGCTTGGCCTTGCCagccgg > 2:248035/1‑138 (MQ=255) tcCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCtt < 2:526323/143‑1 (MQ=255) cTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGa < 1:219804/143‑1 (MQ=255) tgtcGACGGGCGAGCAACTTTGCCTGCAACGTGGAGTCACCGGGATGGGGGTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTGTATCACTTCTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAg < 2:51254/140‑1 (MQ=255) tGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGGCGAAAGGGTCCTTCTCTCGCTGCTTGCGCTCTAAAGAAGCGTTTGGCCGTGCCATGCTGGAg > 1:363604/1‑143 (MQ=255) tGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACCAGTCGGGAACTAAAGAAGCGTTTGGCGTTGCCATGCTTGAg > 1:134087/1‑143 (MQ=255) ggCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGc < 2:303473/143‑1 (MQ=255) cGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGGCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGt < 1:487782/143‑1 (MQ=255) gggCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTGGGCGCTGCCATGCCTGAGCGTCAtt > 2:533730/1‑143 (MQ=255) cGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTAcg > 2:425494/1‑143 (MQ=255) gttGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCatat < 2:402224/142‑1 (MQ=255) ttGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTGCGCCGCATATc > 1:409476/1‑143 (MQ=255) cTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCa > 1:974/1‑143 (MQ=255) tGCAACGTGGATTCACCGGGATGGGGCTAAGCGCATCACCAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCACAGCACCAg > 2:600647/1‑143 (MQ=255) tGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGATCCTTCTATCACTACTTGCGCTGTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCCTTACGCCGCATATCACCAg > 2:196291/1‑143 (MQ=255) tGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGCCCTTCTATAAATGATTGCGCGCTAAAGACGCGTTTGGCTATGCCGCGCTTGGGCGACAGTGAGCCGCATAGGCCCAGCgactga > 2:631261/1‑143 (MQ=255) gATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCCCTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCgactgcct > 1:141731/1‑143 (MQ=255) ttCATCGGTATGGGGCTAAGCGAATTGCTAAAACCCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAACAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGActgactga < 2:185735/143‑1 (MQ=255) cACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGt < 1:465954/143‑1 (MQ=255) cACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGt < 1:465832/143‑1 (MQ=255) ggATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTtgctg < 1:670843/143‑1 (MQ=255) ggATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTtgctg < 1:237515/143‑1 (MQ=255) ggggCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATCTCACCAGCGACTCACTGCGGTGCTGCagt > 2:53928/1‑141 (MQ=255) cGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGGAg > 1:291071/1‑143 (MQ=255) gAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAgg > 2:170196/1‑143 (MQ=255) gAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTAATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAgg > 1:725605/1‑143 (MQ=255) aaaaCCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGCCTGACTGCGTTGCTGCAACCCGGCGAAGTTAACTAcag > 2:110976/1‑141 (MQ=255) aCCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGCAGGTAACTACCGCGa > 1:71624/1‑143 (MQ=255) gCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCg > 1:235004/1‑143 (MQ=255) gTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACt > 1:473924/1‑143 (MQ=255) aGGGTCCTTCCATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCttatt < 1:431978/143‑1 (MQ=255) tCCTTCTATCACTACTTGCGCTCTCACGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCa < 1:694588/143‑1 (MQ=255) tCACTACTTGCGCTCGAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAacac < 1:558135/143‑1 (MQ=255) ctactTGCGCCCTACAGAAGCGTTCGGCGTTCCCATGCTTGACCGCCATTCCGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGa < 1:175399/143‑1 (MQ=255) | CACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGA > NC_000913/1726034‑1726305 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |