Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 1,159,286 +GTA coding (1418/1434 nt) ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 11592822 (0.030)75 (1.170)
+AGT
61/268 0.0 97.4% coding (1414/1434 nt) ptsG fused glucose‑specific PTS enzymes: IIB component/IIC component
?NC_000913 1159283 = 2 (0.030)coding (1415/1434 nt) ptsG fused glucose‑specific PTS enzymes: IIB component/IIC component

AGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATG‑‑GATG‑‑‑AGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTC  >  NC_000913/1159150‑1159424
                                                                                                                                          |                                                                                                                                             
gcagcgttgctgatgtgtctacaggggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggtggctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTAc                                                                                                                                           <  2:67536‑M2/5‑1 (MQ=255)
   gcgttgcggatgtgtctgaagtggataaggccggcctgaagagactgggggcagcgggcgtagtggttggtggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATc                                                                                                                                        <  2:155947‑M2/8‑1 (MQ=255)
    cgttgctgatgtgtctaaagtggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaacgccgagctggatgagcAGTACCTcc                                                                                                                                       >  1:10325‑M2/135‑143 (MQ=255)
     gttgctgatgtgtctaaagtggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgcgatggatgtgtAGTACATCCg                                                                                                                                      >  1:120434‑M2/134‑143 (MQ=255)
     gtggctgatgtgtctaaagtggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCg                                                                                                                                      <  1:285180‑M2/10‑1 (MQ=255)
      ttgctgatgtgtctaaagtggatcaggccggcctgaagggactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGt                                                                                                                                     <  2:898755‑M2/11‑1 (MQ=255)
      tggctgatgtgtctaaagtggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGt                                                                                                                                     <  1:36173‑M2/11‑1 (MQ=255)
        gctgatgtgtcgaaagtggatcaggccggcctgaagaaacggggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTaa                                                                                                                                   <  1:126191‑M2/13‑1 (MQ=255)
         ctgatgtgtctaaagtggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAAc                                                                                                                                  >  1:1019852‑M2/130‑143 (MQ=255)
          tgatgtgtctaaagtggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAAcc                                                                                                                                 >  1:68124‑M2/129‑143 (MQ=255)
          tgatgtgtctaaagtggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAAcc                                                                                                                                 >  2:187554‑M2/129‑143 (MQ=255)
              gtgtctaaagtggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCActc                                                                                                                             >  1:51875‑M2/125‑142 (MQ=255)
              gtgtctaaagtggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTa                                                                                                                             >  1:46884‑M2/125‑143 (MQ=255)
              gtgtctaaagtggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggctgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTa                                                                                                                             >  2:1169631‑M2/125‑143 (MQ=255)
                   taaagtggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCg                                                                                                                        >  1:1071996‑M2/120‑143 (MQ=255)
                   taaagtggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACAAACCCg                                                                                                                        >  2:974877‑M2/120‑143 (MQ=255)
                      agtggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTAGATCCGTAACCACTCATCCGCaa                                                                                                                     >  1:839847‑M2/117‑143 (MQ=255)
                      agggggtcagggcggcctgaagaaggtgggcgcagcgggcgtagtggttgcgggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTaa                                                                                                                     <  2:51822‑M2/27‑1 (MQ=255)
                        tgggtcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGa                                                                                                                   <  2:478155‑M2/29‑1 (MQ=255)
                        tggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGa                                                                                                                   >  1:172387‑M2/115‑143 (MQ=255)
                               tgccgcgctgaagcaacggggcgtagcgggcgtagtggttgctggttcgggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTggg                                                                                                            <  2:55855‑M2/36‑1 (MQ=255)
                                gccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactgaatacgataacctgaaaaccgagatggatgagtAGTACAGACGTAACCACTAATCCGTGAGACgctgaga                                                                                                           >  2:847604‑M2/107‑140 (MQ=255)
                                gccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTgggg                                                                                                           >  1:419350‑M2/107‑143 (MQ=255)
                                 ccggcctgaagaaactgggcgcagcgggcgtagtggttgcgggttctggtgttcaggcgattatcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGga                                                                                                          <  1:600399‑M2/38‑1 (MQ=255)
                                  cggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTCACCACTAATCCGTACGACGTTGGGgag                                                                                                         >  1:1081676‑M2/105‑143 (MQ=255)
                                     cctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTCCATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACt                                                                                                      >  1:947883‑M2/102‑143 (MQ=255)
                                       cgaagaaactggtcgcagcgggcgtaggagttgctggtgcgggtgttcaggcgattttcggtactaactccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTaa                                                                                                    <  1:90044‑M2/44‑1 (MQ=255)
                                               ctgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCa                                                                                            >  1:689685‑M2/92‑143 (MQ=255)
                                                    cgcagtgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATgg                                                                                       <  1:431664‑M2/57‑1 (MQ=255)
                                                      cagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCt                                                                                     <  1:378823‑M2/59‑1 (MQ=255)
                                                      cagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCt                                                                                     >  1:880943‑M2/85‑143 (MQ=255)
                                                            gcgtagtggttgctggttctggtgttccggcgattttcggtactaaatccgataacctgaacaccgagatggctgagtAGTACATCCGTAACCACTCAGACGTAAGACGAGGGGGAGACTAAGGCAGCCagctggcggcgtat                                                                               >  2:941759‑M2/79‑139 (MQ=255)
                                                            gcgtagtggtgggtggttctggtgttcaggcgattttcggtactgaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCATCTGGCTGCctta                                                                               <  2:574173‑M2/65‑2 (MQ=255)
                                                               tagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCtttttt                                                                            <  1:719070‑M2/68‑1 (MQ=255)
                                                                  tggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACa                                                                         <  1:187554‑M2/71‑1 (MQ=255)
                                                                    gttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGCCGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAgg                                                                       >  2:341636‑M2/71‑143 (MQ=255)
                                                                      tgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTCTTCTACAGgtg                                                                     >  1:683272‑M2/69‑143 (MQ=255)
                                                                       gctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGgtgt                                                                    <  2:505923‑M2/76‑1 (MQ=255)
                                                                       gctggttctggggtttacgtgatttgcggttctaaatccgataaccttaaaaccgagatggattagtAGTACATCCGTAACCACGAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGgtgt                                                                    <  2:505922‑M2/76‑1 (MQ=255)
                                                                       gctggttctggcgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTACGGCAGCCAGATGGCTGCCTTTTTTACAGgtgt                                                                    <  2:505926‑M2/76‑1 (MQ=255)
                                                                          ggttctggtgtgcaggggattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTAt                                                                 <  2:159984‑M2/79‑1 (MQ=255)
                                                                           gttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTAtt                                                                <  1:402687‑M2/80‑1 (MQ=255)
                                                                           gttctggtctgcatgcgagtttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGTGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTAtt                                                                <  2:949918‑M2/80‑1 (MQ=255)
                                                                             tgtggtgttcaggcgatcttccgtactaaacccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCa                                                              <  1:729214‑M2/82‑1 (MQ=255)
                                                                             tcgggtgttcaggcgattgtcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCa                                                              <  1:291879‑M2/82‑1 (MQ=255)
                                                                               tggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCGGTAAACACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCCGATGGCAGCCTTTTTTACCGGTGATATCCAGa                                                            >  2:597962‑M2/60‑143 (MQ=255)
                                                                                ggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGaa                                                           <  1:755341‑M2/85‑1 (MQ=255)
                                                                                  tgttcaggcgattttccgtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGACTGCCTTTTTTACAGGTGTTATTCAGAAtt                                                         <  2:569828‑M2/87‑1 (MQ=255)
                                                                                   cttccggcgattttcggtactacatccgatgacctgaaaaccgggatggatgggtAGGACATCCGTAACCACTAATCCGTCAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTg                                                        <  2:875111‑M2/88‑1 (MQ=255)
                                                                                    ttcatgcgattttcggtactaaatctgataaactgaaaatcgagatggatgagtAGTACATTCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGa                                                       <  1:392469‑M2/89‑1 (MQ=255)
                                                                                     tcaggcgattttcggtactaaatccgataacatgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCGGAAAGGGAGCTGCCTTGTTTGCAGGTGGTATGCACAATTGAt                                                      >  1:803288‑M2/54‑143 (MQ=255)
                                                                                      caggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGCTGGACGCCTTTTTTACAGGTGGTattcagaagtacta                                                     >  2:351194‑M2/53‑137 (MQ=255)
                                                                                       aggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAACCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATAc                                                    >  1:346584‑M2/52‑143 (MQ=255)
                                                                                         gcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTCTTCAGAATTGATACGt                                                  >  1:666245‑M2/50‑143 (MQ=255)
                                                                                            attttcggtactatatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGcc                                               <  1:304499‑M2/97‑1 (MQ=255)
                                                                                               tgcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGt                                            <  1:528189‑M2/100‑1 (MQ=255)
                                                                                                   gtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACgtgccggaactg                                        >  1:893115‑M2/40‑138 (MQ=255)
                                                                                                     tAAATCCGATCAACTAAAAACCGAGATG‑‑GATGAGTAGTACATACGTAACCACGAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCAGCCTGTTTAACAGGGATTATTCAGAATTGATACGAGCAGGGAAtcatga                                    >  2:87821/1‑138 (MQ=255)
                                                                                                     tAAATCCGATAACCTGAAAACCCAGATGAAAATGC‑‑ACTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCACCCAGATAGCTGCCTCTCTTACAGGTGTTACTCAGAATGGAGACGCGCTGGCAACGcgtc                                    >  2:86999/1‑140 (MQ=255)
                                                                                                       taactccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGa                                    <  1:849723‑M2/108‑1 (MQ=255)
                                                                                                       taaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGAGGAGACTAAGGCAGCAAGATGGCTGCCTTGTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATgctaa                                    >  2:322518‑M2/36‑141 (MQ=255)
                                                                                                        aaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGaa                                   <  2:930099‑M2/109‑1 (MQ=255)
                                                                                                          atccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAAt                                 >  2:426573‑M2/33‑143 (MQ=255)
                                                                                                          atccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAAt                                 <  1:520270‑M2/111‑1 (MQ=255)
                                                                                                            gcgatgagctgaaaggcgagatggatgagtAGTAGATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCCGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTa                               <  1:406757‑M2/113‑1 (MQ=255)
                                                                                                               ataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTAcgc                            <  1:3478‑M2/116‑1 (MQ=255)
                                                                                                                  accttaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGgt                         <  1:275515‑M2/119‑1 (MQ=255)
                                                                                                                     tgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGAAGTTGGGGAGGCTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGtgtg                      >  1:998656‑M2/22‑143 (MQ=255)
                                                                                                                        aaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCg                   <  2:172387‑M2/125‑1 (MQ=255)
                                                                                                                         aaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGCGACTACGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTCATACGTGCCGGTAATGCTCAAAGTCAGCGGTCTGCCGt                  >  2:386824‑M2/18‑143 (MQ=255)
                                                                                                                         aaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGGTGGGGAGACTCAGGCAGCCAGATGCCTGCCTTTTTTACAGGTGTTATTCCGACTTGATACGTGCCGGTAATGCGGACGTTACGCGAGGTGCCGt                  >  2:1055288‑M2/18‑143 (MQ=255)
                                                                                                                         aaccgagatggatgagtAGTACATCCGTAACCACTAATCAGTAAGACGGTGGGGAGACTACGGCAGCCAGATGGGTGCCTTTTTTACAGGGGTTATTCGGAATTGAGACGTGCCGGGAATGCTGAAATtacgcggtgctccgc                  >  2:1067391‑M2/18‑137 (MQ=255)
                                                                                                                          accgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTa                 <  1:1028229‑M2/127‑1 (MQ=255)
                                                                                                                           ccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAGTGCTGAAATTACGCGGTGTACCGTAg                >  2:493004‑M2/16‑143 (MQ=255)
                                                                                                                              agatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTagagg             >  1:569469‑M2/13‑141 (MQ=255)
                                                                                                                                    atgggtAGTACATCCGTAACCACTAATCCGTAAGACGGAGGGGAGACTAAGGCAGCCAGAAGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGGGCCGTGGACGATaaaa       >  2:55083‑M2/7‑143 (MQ=255)
                                                                                                                                    atgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTCAAATTACGCGAGGTGCCGTAGACGATAGaa       >  1:1113183‑M2/7‑143 (MQ=255)
                                                                                                                                    atgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGAGTTATTACGACTCGATGCGGTCTGGTATTGCTGAAATTACGCGGTGTGACGTCGACGCTAGaa       >  2:1090021‑M2/7‑143 (MQ=255)
                                                                                                                                       ggtAGTACATCGGTTACCGCTGATCCGTAAGGCGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCt    <  1:1153942‑M2/140‑1 (MQ=255)
                                                                                                                                       agtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCt    <  1:1067814‑M2/140‑1 (MQ=255)
                                                                                                                                        gtAGTACATCCGTAACCACTAATCCGTGGGTCGTTGAGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCtt   <  2:10325‑M2/141‑1 (MQ=255)
                                                                                                                                         tAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTc  <  2:658502‑M2/142‑1 (MQ=255)
                                                                                                                                         tAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTc  >  1:708496‑M2/2‑143 (MQ=255)
                                                                                                                                          |                                                                                                                                             
AGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATG‑‑GATG‑‑‑AGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTC  >  NC_000913/1159150‑1159424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.