Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1299499–1300697 1300697 1–1199 58 [0] [0] 57 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)54 (0.850) 44/274 0.5 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

CCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACG  >  NC_000913/1300563‑1300838
                                                                                                                                       |                                                                                                                                             
gttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagcataattgaaatgatattattaattccactgcctttggtagaggaaagtgcgaaataataat                                                                                                                                        >  2:449877‑M2/136‑143 (MQ=255)
   tgttataaggatttccttaactgcttctcctcaccgtcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCaa                                                                                                                                     <  2:616970‑M2/11‑1 (MQ=255)
     ttataaggctttccttaactgcttctcctcaccatcatgttattttcgccacatcctaatcctggccttcctgacgaataattgaaatgatattattaattcccctgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                   >  2:461179‑M2/131‑143 (MQ=255)
     ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                   <  2:1062435‑M2/13‑1 (MQ=255)
       ataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATtgc                                                                                                                                 >  2:650968‑M2/129‑142 (MQ=255)
        taaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGtt                                                                                                                                >  1:307445‑M2/128‑143 (MQ=255)
             atttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctcggcttgctgcagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAACAATCAATTCTTAaatc                                                                                                                           >  2:963804‑M2/123‑142 (MQ=255)
                tccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                        <  1:228373‑M2/24‑1 (MQ=255)
                tccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                        <  1:247760‑M2/24‑1 (MQ=255)
                   ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaataatattattaattccactacctttgatatatgaaaatgctaaATAATAATCAATAGTTAAATTATtttg                                                                                                                     >  2:74221‑M2/117‑143 (MQ=255)
                          cttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCa                                                                                                              <  1:123184‑M2/34‑1 (MQ=255)
                          cttctcctcaccatcatgttattttcgccacagcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCa                                                                                                              <  1:491517‑M2/34‑1 (MQ=255)
                               cctcaccatcatgttattttcgccgcatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCactact                                                                                                         >  1:873565‑M2/105‑143 (MQ=255)
                                ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTg                                                                                                        >  1:1057979‑M2/104‑143 (MQ=255)
                                     catcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACt                                                                                                   >  2:202534‑M2/99‑143 (MQ=255)
                                        catgttattttcgccacatcatactcctgggcttgctgaagaataattgaaatgatattatcaattccactgcctttggtagaggaaagtgctaaAATATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTa                                                                                                <  1:840298‑M2/48‑1 (MQ=255)
                                          tgttatttgcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAt                                                                                             <  2:157593‑M2/50‑1 (MQ=255)
                                          tgttattgtcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAt                                                                                             <  1:176315‑M2/50‑1 (MQ=255)
                                           gttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                            >  1:889890‑M2/93‑143 (MQ=255)
                                           gttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                            >  1:84577‑M2/93‑143 (MQ=255)
                                           gttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattataaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                            >  1:79929‑M2/93‑143 (MQ=255)
                                            ttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCa                                                                                           >  1:1095125‑M2/92‑143 (MQ=255)
                                               ttgtcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagacgaaagtgctaaATAATAATCAATTGTTAAATTATTGATCATTTCACTACTAGAACTATAATCAGaa                                                                                        >  2:1135261‑M2/89‑143 (MQ=255)
                                                tttcgtcacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaa                                                                                       <  2:1031362‑M2/56‑1 (MQ=255)
                                                      cacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAg                                                                                 <  1:477655‑M2/62‑1 (MQ=255)
                                                      cacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACCACTGAAACTGTAATCAGAAAAGATAg                                                                                 >  2:445200‑M2/82‑143 (MQ=255)
                                                       acatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGa                                                                                <  1:682467‑M2/63‑1 (MQ=255)
                                                       acatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGa                                                                                >  1:233415‑M2/81‑143 (MQ=255)
                                                         atcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACa                                                                              <  1:983920‑M2/65‑1 (MQ=255)
                                                           cataatcctgcgcttgcgtaagaataattgaaattatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATg                                                                            <  1:189841‑M2/67‑1 (MQ=255)
                                                            ataatcctgggcttgctggagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGc                                                                           <  1:218339‑M2/68‑1 (MQ=255)
                                                                tcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAg                                                                       >  2:741333‑M2/72‑143 (MQ=255)
                                                                     ggcttgcggaagaataattgaaatgagattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAt                                                                  <  2:8943‑M2/77‑1 (MQ=255)
                                                                            tgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTATAATCAGAAAAGATAGACATGCATAGCCACTCTCGAtt                                                           >  2:911517‑M2/60‑143 (MQ=255)
                                                                             gaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAttt                                                          <  1:837090‑M2/85‑1 (MQ=255)
                                                                             gaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAttt                                                          <  1:193907‑M2/85‑1 (MQ=255)
                                                                               agaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttga                                                        <  2:119782‑M2/87‑1 (MQ=255)
                                                                               agaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttga                                                        <  2:647208‑M2/87‑1 (MQ=255)
                                                                                 aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                      >  2:590232‑M2/55‑143 (MQ=255)
                                                                                    aattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaa                                                   <  1:1137836‑M2/92‑1 (MQ=255)
                                                                                         aaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGAgtgacttgaa                                              >  2:1076743‑M2/47‑143 (MQ=255)
                                                                                          aatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                             <  1:217266‑M2/98‑1 (MQ=255)
                                                                                              atattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtg                                         >  2:541039‑M2/42‑143 (MQ=255)
                                                                                                attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGCTAGACATGCTTAGCCAATATCTATTTGATTGAACTGAAAGAtgtt                                       >  1:384529‑M2/40‑143 (MQ=255)
                                                                                                attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtt                                       >  1:384534‑M2/40‑143 (MQ=255)
                                                                                                attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtt                                       >  1:384535‑M2/40‑143 (MQ=255)
                                                                                                attagtaattccacgggctttggtagaggaaagtgctaaATAATAATCAAGTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtt                                       <  2:1119257‑M2/104‑1 (MQ=255)
                                                                                                      aattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaa                                 <  2:1183593‑M2/110‑1 (MQ=255)
                                                                                                       attccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTACATCGTACTAAGAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                                >  1:547145‑M2/33‑143 (MQ=255)
                                                                                                        ttccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAgg                               >  1:189734‑M2/32‑143 (MQ=255)
                                                                                                           cactgcctttggtcgaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCAt                            <  2:1095125‑M2/115‑1 (MQ=255)
                                                                                                           cactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCAt                            >  1:412047‑M2/29‑143 (MQ=255)
                                                                                                                  tttggtagaggaaagtgctacATAATAATCAATTGTTAAATTATTGTGCATTTAACAACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCa                     >  1:345402‑M2/22‑143 (MQ=255)
                                                                                                                   ttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCaa                    <  1:706152‑M2/123‑1 (MQ=255)
                                                                                                                                   ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATa    <  2:1057979‑M2/139‑1 (MQ=255)
                                                                                                                                    taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAGTTGAAAGATGTGTGTTAAGGCATGGATGCAAGCTATAGATTCTGAta  >  1:726923‑M2/4‑142 (MQ=255)
                                                                                                                                     aaATAATAATCAATTGTTAAATTATTGTGCATTTCACCACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCGGTCGCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACg  <  1:445200‑M2/141‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                             
CCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACG  >  NC_000913/1300563‑1300838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.