Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,976,909	A→C	E104A (GAG→GCG)	galR →	galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,976,909	0	A	C	100.0%	180.0 / NA	55	E104A (GAG→GCG)	galR	galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (29/26);  total (29/26)

GACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGG  >  NC_000913/2976769‑2977047                                                                                                                                             |                                                                                                                                           gACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTGGGTGCAATGGTGAAAGCGGTCGAGCAGGTGGCTTATCACACCGGTAGTAATTTACAGATTGGGGACGGGTACCACCACGAACAAAAAgcgc                                                                                                                                          >  1:804146/1‑143 (MQ=255)   ccACTGAACCGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCGTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGt                                                                                                                                        <  2:430684/143‑1 (MQ=255)    cACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGATTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTc                                                                                                                                       >  1:597175/1‑143 (MQ=255)    cACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGACTTATCATACCTGTGATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTc                                                                                                                                       >  2:710958/1‑143 (MQ=255)      cTGAAACGGTCGGTCTGGTCGTTGGTGCTGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAg                                                                                                                                     <  1:918312/143‑1 (MQ=255)      cTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGCAATTTTTTATTGATTGGCAACGGTTACCACAACGACCACAAAGCGCGTCAg                                                                                                                                     >  1:198846/1‑143 (MQ=255)            cggtcgtgCTGGGCGGGGGGGGTGTTTCCGAGCCGGTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCAtt                                                                                                                               <  2:90365/143‑1 (MQ=255)            cggtcggtCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCTGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCAtt                                                                                                                               <  2:121640/143‑1 (MQ=255)                   tcTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCCTTGAGCAAc                                                                                                                        >  1:840121/1‑143 (MQ=255)                        tCGTTGGTGAGGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATc                                                                                                                   <  1:724337/143‑1 (MQ=255)                        tCGTGGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTGATCACACCGGTAAGTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATc                                                                                                                   <  1:466462/143‑1 (MQ=255)                          gTTGGTGATGTTTCCGATCCGTTTTTAGGTGCAATGGTGAAAGCGGTCGAACCGGTGGCTCATCACACCGGTAATTTTTTATTGATTGGCAACGGGTAACACAACGAACAAAAAGCGCGTCAAGCAAGTGAATAACTGCTCCg                                                                                                                 >  2:989257/1‑143 (MQ=255)                          gTTGGGGATGTTTCCGATCCGTTTTGCGGTGCAATGGTGCAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCg                                                                                                                 <  2:899160/143‑1 (MQ=255)                                  tGTTTCCGATGCGTTTTTTCTAGCATTGGTGAAAGCTGTCGAACAGGTTGCTTATCACACCGGTAATTTTTTATTGATTTTCAACGCGTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCt                                                                                                         <  2:185874/143‑1 (MQ=255)                                  gtgttccgttccgTTTTTCGGTGCATTGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCt                                                                                                         <  2:1041950/140‑1 (MQ=255)                                    tttCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCACCTGATCCGCCATCGCtgt                                                                                                       >  1:980360/1‑143 (MQ=255)                                     ttCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCtgtg                                                                                                      <  1:417910/143‑1 (MQ=255)                                         gATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCGTCGCTGtgctgc                                                                                                  >  1:53141/1‑143 (MQ=255)                                           tCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGt                                                                                                >  1:414320/1‑143 (MQ=255)                                                gtttCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGgtc                                                                                           <  1:609445/142‑1 (MQ=255)                                                     ggTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCa                                                                                      <  2:611219/143‑1 (MQ=255)                                                      gTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCAt                                                                                     >  1:180654/1‑143 (MQ=255)                                                       tGCAATGGTGCAAGCGCTCTAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATg                                                                                    <  1:156710/143‑1 (MQ=255)                                                       tGCAACGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATg                                                                                    <  1:1150769/143‑1 (MQ=255)                                                         cAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCGTCGCTGTGCTGCGTGGGTCGCCCATGcc                                                                                  >  2:58018/1‑143 (MQ=255)                                                             ggggAAAGCGGTCGAACAGGTGGCTGATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCaaaa                                                                              <  2:147279/140‑1 (MQ=255)                                                               tGAAAGCGGTCGAACAGGTGGCTCATCACACGGGTAATTTTTTATTGATTGGCAACGGTTACGACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATAGCTGTGCTGCGTTGGTCGTGCAGGCCGAAATg                                                                            >  2:331234/1‑143 (MQ=255)                                                                   aGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATcc                                                                        <  2:1079732/143‑1 (MQ=255)                                                                            aCAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGGCGATGCCAAAATGATACCGGATGCTg                                                               >  1:505875/1‑143 (MQ=255)                                                                              aGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGCTCCCGGATGCTggt                                                             >  2:781931/1‑141 (MQ=255)                                                                                          acacCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGGAACTGATCCGCCATCGCTGTGCTGGGTTGGTCGTCCATGCCAAAATGCTCCCGGATGCCGCTTTAGCCTCATTa                                                 >  2:541611/1‑143 (MQ=255)                                                                                          acacCGGTAATTTTTTATTGAATGGCAACGGTTACCACACCGAACAAAAAGCGCGTCAGGCCATTGAGCCACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAACATGATCCCGGATGCTGATTTCGCCTCAtga                                                 >  1:472836/1‑141 (MQ=255)                                                                                                gcaaTTTTGTATTGATTGGCAACGGGTACCACAACGAACAAAGAGCGCGTCAGGCCATTGAGCAACTGAGCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGaaa                                           <  1:32020/141‑1 (MQ=255)                                                                                                 tAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAAc                                          >  1:883428/1‑143 (MQ=255)                                                                                                  aaTTTTTTATTGATGGGCAACGGTTACCACAACGACCCACCAGCATGCCAGTCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACa                                         <  1:859629/143‑1 (MQ=255)                                                                                                  aaTTTTCTATGGATCGGCAACGGTTACCACAACGACCCATCCCCGCGTCAGGCCCTTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACa                                         <  1:859854/143‑1 (MQ=255)                                                                                                   aTTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGCTTTAGCCTCATTAATGAAACaa                                        >  1:222803/1‑143 (MQ=255)                                                                                                     tttttATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCCACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAGAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACaacg                                      >  2:471109/1‑141 (MQ=255)                                                                                                           ttgattgGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCgg                                >  1:633184/1‑143 (MQ=255)                                                                                                             gattgGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTa                              >  2:642626/1‑143 (MQ=255)                                                                                                             gatggGCAACGCTCACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTa                              <  1:191208/143‑1 (MQ=255)                                                                                                               ttgGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGATGATTTAGCCTCATTAATGAAACAAATGCTCGGTATg                            >  2:745994/1‑143 (MQ=255)                                                                                                                tcgCAACGGTCACCACAACGAACCAACAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATgg                           <  1:593515/141‑1 (MQ=255)                                                                                                                  gCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTTATCCGCCATCGCTGTGCTGCGTTGGTCGTCCAGGCCAAAATGATCCCGGATGCTGATTTCGCCTCATTAATCAAACAAATGCCCGGTATGGTg                         >  2:363746/1‑143 (MQ=255)                                                                                                                  gCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATGCAGGATGCTGATTTAGCCGCATTCATGAAACAACTGCCCGGTATGGTg                         >  2:1089458/1‑143 (MQ=255)                                                                                                                    aaCGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATCAATGAAACAAATGCCCGGTacgccgct                       >  1:223460/1‑136 (MQ=255)                                                                                                                       cgTTACCACAACGAACAAAAAGCCCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTTCTGCTTTGCTCTTCCATGCCCAAATGTTCCCGGATGCTTATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGAt                    <  1:326432/142‑1 (MQ=255)                                                                                                                           actagagcGAACAATTAGCGCGTCAGGGTATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTTGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAAc                <  2:36944/136‑1 (MQ=255)                                                                                                                             cacaACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCg              >  2:409808/1‑143 (MQ=255)                                                                                                                             cacaACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGATATGGTGCTGATCAAcag              >  1:65547/1‑141 (MQ=255)                                                                                                                             cacaACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGAGTTCGCCTCATTAATGAAACAAATGCCCAGAAAGGGTCTGATCAACCg              >  2:1018145/1‑143 (MQ=255)                                                                                                                                aaCGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAACTGCCCGGTATGGTGCTGATCAACCCTAt           >  2:330515/1‑143 (MQ=255)                                                                                                                                 aCGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATc          <  2:920129/143‑1 (MQ=255)                                                                                                                                   gAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATcct        >  1:1069369/1‑143 (MQ=255)                                                                                                                                        aaaaGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATcctgcctg   <  2:1069369/143‑1 (MQ=255)                                                                                                                                         aaaGCGCGTCAGGCCAGTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTgg  <  1:561159/143‑1 (MQ=255)                                                                                                                                             |                                                                                                                                           GACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGG  >  NC_000913/2976769‑2977047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑