| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,726,876 | C→T | P73L (CCT→CTT) | nemA → | chromate reductase, quinone reductase, FMN‑linked; N‑Ethylmaleimide reductase; old yellow enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,726,876 | 0 | C | T | 97.9% | 133.8 / NA | 48 | P73L (CCT→CTT) | nemA | chromate reductase, quinone reductase, FMN‑linked; N‑Ethylmaleimide reductase; old yellow enzyme |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (28/18); minor base G (0/1); total (29/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCGCAGTATTGAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGC > NC_000913/1726744‑1727014 | tGCGCAGTATTGAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCCTGGCATGCac > 2:695853/1‑142 (MQ=255) cgcAGTATTGAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCCACACGATCTGCAGGTGCGCTTGGCCTCCGTAg > 1:213794/1‑143 (MQ=255) ttGAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGCATATGCAGGTGCGCTAGGCATCCATAGTCCGGAg > 2:1006594/1‑143 (MQ=255) aTTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTg < 1:181426/112‑1 (MQ=255) aTTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTg > 2:181426/1‑112 (MQ=255) aCCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGGGCGCTTGGCATCCATAGGCCGGAGCACGTGGCCGCATGGaaaaa > 1:309324/1‑143 (MQ=255) cccGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGaaaaaaa > 2:270746/1‑143 (MQ=255) cccGGTGATGGCGGAACACTATCGCCAACTTGCGAGTGCCGGTTTTATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGTAAAAGGATATGCAGGTGCGCTTGGCATCCGTAGTCCGGAGCAAATTGCCGCATGGaaaaaaa < 1:995600/143‑1 (MQ=255) aTGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGc > 1:682522/1‑143 (MQ=255) cTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGAATCCATAGGCCGGAGCAAATTGCCGCATGGAAAAACAACACAGCTGGTGTTCATg > 2:631835/1‑143 (MQ=255) tgtCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGc < 2:309432/141‑1 (MQ=255) tATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATGCATAGTCCGGAGCAAATTGCGGCAGGGAGGAAAATCACCGCTGGCGGTCGTGc > 2:1056810/1‑143 (MQ=255) tATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGc > 1:1149042/1‑143 (MQ=255) tATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGACCCAAAGGCAACAAAGTTAGAAGTCACCGCTGGCCTTCACGc > 1:492301/1‑143 (MQ=255) tATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCCCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGc > 1:992740/1‑143 (MQ=255) aTCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGGTCATGCt > 2:679411/1‑143 (MQ=255) aaCGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAAt < 1:430590/143‑1 (MQ=255) aaCGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCGGGCGCTCATGCGGaacaa > 1:729356/1‑140 (MQ=255) aCGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATg < 1:635955/143‑1 (MQ=255) cGTGACAGTGCCGGTTGGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATgg < 1:340685/143‑1 (MQ=255) tGCCTGTGCCGGGTTGATTATTACTGAAGCCACGCAAATTTCTGCTCAGGCAACAGGATTTGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATAGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTc < 2:583552/143‑1 (MQ=255) gCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCa < 1:624601/143‑1 (MQ=255) tGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCAGAGGAGATAATAGTTTGGTAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATgg > 1:862591/1‑143 (MQ=255) ccGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCAGGGAAAAAAATCACGGCTGGCGTTCATGCTGAAAATGGTCATATGGcc < 1:955684/143‑1 (MQ=255) ccGGTTTGATTATTAGTGAAGCCACGCAAAATACTACCCATTCAAAAAACTGACCCGGTGCGCTTTTCATCCATAGTCCGGAGCAAATGGCCGCAGGGAAAAAAATCAACGCTGTCGTGCATGCCGAACAGGGTCAGATGGcc > 2:1101140/1‑143 (MQ=255) gTTTGATTATGAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGGTATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTg < 1:44267/143‑1 (MQ=255) tGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAACCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAg > 2:817902/1‑143 (MQ=255) ttaGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTGCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCAGGCTGAAAAGGGTCATAGGGCCGTGCAGCTGTgg > 2:612275/1‑143 (MQ=255) ttaGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTgg < 1:838049/143‑1 (MQ=255) aaGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCAGGAGCAAATTGCCGCATGGAAAAAAATCACCGCACGCGCTCGCGCGGACAATGATCATATGGCCGTGCAGCTGTGGCACAcc > 2:1021532/1‑143 (MQ=255) aGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATAGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCg > 1:708989/1‑143 (MQ=255) ccACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGa > 1:1013894/1‑143 (MQ=255) aCGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACg > 1:636323/1‑143 (MQ=255) cGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGCTCATATGGCCGTGCAGCTGTGGCACACCGGACGc > 1:365206/1‑143 (MQ=255) aaTTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCACATTGCCGCATCGAAAAAACTCACCGCCGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCAttt < 2:106543/143‑1 (MQ=255) ttccTGCCCAGGCAAAAGGATATGCACGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAACATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTtct < 2:909326/140‑1 (MQ=255) ttCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTtctc < 2:682522/143‑1 (MQ=255) tGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATGCCTCACg > 2:341727/1‑143 (MQ=255) ggCAGAAGGATAGACAGGGGCGCGTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCGGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGcc < 2:459679/143‑1 (MQ=255) cAAAAGGATATGCAGGTGCGCTTGGCACCCACAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTg < 2:992740/143‑1 (MQ=255) aaaaGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGc > 2:612288/1‑143 (MQ=255) aaGGATATGCAGGTGCGCTTGGCCTCCATAGTCAGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGCTCATATGGACGTGCAGCAGTGGCACACCGGACGCATATCCCACGCCAGCCCGccc > 2:158931/1‑141 (MQ=255) aaGGATATGCAGGTGCGCTTGGCATCCAGAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCaa < 1:242761/143‑1 (MQ=255) ggATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATAGCCGTGCAGCTGTGGCACACCGCAAGCCTATCTCACGCAAGCGTGCAAca > 2:564865/1‑142 (MQ=255) atatGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAACATATGTACTAGCGGTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTg < 1:270746/143‑1 (MQ=255) tatGCAGGTGCGCTTGGCATCCATAGTCCGGAGCACATTGCCGCCTGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAACGGTCATATGGCCGTGCACCTGTGCCACACCGGACGCATTTCTCCCGCCAGCCTGccggctgg > 2:403204/1‑136 (MQ=255) atGCAGGTGCACTTGACATCCATAGTCCGGATCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGc < 2:414537/143‑1 (MQ=255) gcgcTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGc > 1:1054233/1‑143 (MQ=255) | TGCGCAGTATTGAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGC > NC_000913/1726744‑1727014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |