| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,560,455 | 1 | . | G | 97.9% | 142.3 / NA | 48 | intergenic (‑2/+1) | glpR/glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Reads supporting (aligned to +/- strand): ref base . (0/1); major base G (25/21); minor base T (0/1); total (25/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACG > NC_000913/3560314‑3560590 | tCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGCGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGcg > 2:372415/1‑142 (MQ=255) aaaCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGa > 2:438179/1‑143 (MQ=255) cAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCa < 1:202913/143‑1 (MQ=255) aGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGGGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAg < 2:541062/143‑1 (MQ=255) tCGAGCCATTGGGGATTTGCTCCGCCACTGTGCGGGCGATGCGCTCGTTTTCTTCGGTCTGGGTGTCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGttt < 1:118669/143‑1 (MQ=255) gAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CGT‑GATGGCGCAGGATCAGGGTTTg > 1:862407/1‑143 (MQ=255) ccATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACTGATCTGGGAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTc > 1:720656/1‑143 (MQ=255) cagtGGGGATGGGCGCCGCCACTTTGCGGGCGAGGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTcc < 1:293090/140‑1 (MQ=255) gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAg < 1:561381/143‑1 (MQ=255) gggATCTGCTCCGCCCCTCGGCGGGCGATGCGCTCTTTTTCTTCGGCCTGGGTGGCCTTGCGCTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAg < 1:50254/143‑1 (MQ=255) ggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCGCCGCCAGc > 1:75211/1‑143 (MQ=255) gcgtttCCTCCGCCTCTCTGCGGACGATGCGCTCTTGTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGc < 2:858479/140‑1 (MQ=255) gctttGTTCCGCCACTTTGCGGGCGCTGCGCTCTTTTTCTTCGGGCTGGGTGGCCTTGCGATCGTGCCCCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCt < 2:274906/141‑1 (MQ=255) tGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGAGCCGGTTTTGCTCCGCCAGCTCAtt > 1:268007/1‑143 (MQ=255) tGCTCCGCCACTTTGCGGGAGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCAAAGGCGTGTTAAACGAACTGGAAGGCAGCGCCGCAC‑CGT‑CTT‑GATGGCGCAGGAGCAGGTTTTGCTGCGCAAGCTCaca > 2:159229/1‑141 (MQ=255) cgCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt < 1:466670/142‑1 (MQ=255) cTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg < 1:216152/143‑1 (MQ=255) tGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGGTTTGCTCCGCCAGCTCCTTGAGGGCGCGGCGa > 2:539316/1‑143 (MQ=255) tctttctTTTTCTTTGGTCTGTGTGGCCTTGTGTTGGTTCTACGGCTTTTTTACCTAATCGGTAGGTAGTGCCGCTT‑TGC‑CAT‑TATGGCGCAGGATCAGGTTTTGCTCCGTCAGCTCATTGAGTTCGCGGCTAATAGTCttct < 2:424958/139‑4 (MQ=255) gcgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGACGGCAGCGCGGCGC‑CGC‑CAT‑GATGGCGCAGGGTCAGGTGTTGCTCCGACGGCTCATTGAGGTCACGGGGAATAGTCTGCg > 2:772921/1‑143 (MQ=255) gcgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAATGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCATCTCATTGAGGTCGCGGCGAATAGTCTGCg > 1:206091/1‑143 (MQ=255) gcgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACAGGCCG‑‑ATCCCTGGTCTCGCTGAT‑GTTGGCTCACTACCACCCTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCg < 1:632837/143‑1 (MQ=255) cgcTCTGTGCCTTCGGTCCGGGGGGCCCTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg < 1:654318/143‑1 (MQ=255) gcTCTTTTTCGGCGGCCTGGGTGGCCGTGCGATCGTGCCACGGCGTGTCAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCggg < 1:3646/143‑1 (MQ=255) ctctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCGTCGCGGTCGCGGCGATTGGTCTGCGGGc > 1:340444/1‑143 (MQ=255) ttttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGGACTGGAAGGCAGCGCGGCAC‑CGC‑CAG‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGGGGTCGCGGCGAATAGTGTGCCGGCTgca > 2:541721/1‑141 (MQ=255) ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAGGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGAGGTCGCGGCGAATAGTCTGCGAGATGACGgaga > 1:139487/1‑143 (MQ=255) gcgggcggggTGGCCTTGGCAGCGTGCCACGGGGTGTTATCCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CATGGGGGGG‑CAGGTTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaa < 2:650709/136‑1 (MQ=255) ggTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAt < 1:460564/143‑1 (MQ=255) tCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGGAATGc > 2:735744/1‑143 (MQ=255) ggggCCTTGCGACCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAc < 2:127607/141‑1 (MQ=255) gCGATCGTGCCACTGTGTGTTAACCGAACTGGGAGGCAGCGCCGCCC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt < 2:459076/143‑1 (MQ=255) gagcGTGCCGCGGCGTGCTACGCTAACTGGAAGGCGGCGCCGCAC‑CGC‑CAT‑GAGGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCCGCTCTTCg < 2:862407/140‑1 (MQ=255) gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGGAAGGCGCTACCAGCCCTTCg > 1:327843/1‑143 (MQ=255) tCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGACTAGTCTGCGGGCTCACGGAGAAATGCTCTACCAGCCCTTCGGt > 1:109468/1‑143 (MQ=255) tCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt > 1:610397/1‑143 (MQ=255) tGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACt > 1:418549/1‑143 (MQ=255) cGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAc < 2:359613/143‑1 (MQ=255) aaCCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGAGCAGGTTGTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAACGGCTCTGCGAGCTCTGCGGGACTGACATGACCctgcg > 2:145477/1‑142 (MQ=255) aCTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTaa < 1:346724/143‑1 (MQ=255) cTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc > 1:817586/1‑143 (MQ=255) ggAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGAGGTCGCGGCGAATAGTCTGCCGGCTGACGGAGACATGCTCTACCACCCCTTCGGTACTGCCATAACCCTGCTGTCTAACCa > 2:824303/1‑143 (MQ=255) aGGCGGCGCCGGCC‑CGG‑CCT‑GATGGCGCGGGGTCAGGTGTTGCTCCGCCAGCGCATTGAGGTCGCGGCGAATAGTGTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt < 2:290372/143‑1 (MQ=255) ggCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGACTAGTCTGCGGGCTGTCGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATACGCCGACTGTTGACCCCGTTc > 1:463754/1‑143 (MQ=255) gCAGCGCCGCAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGCTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCCGCTCCTCGGTACTGACATAACCCCGCTGCTTAACCAGCTCg > 2:429566/1‑143 (MQ=255) caccgccgcAC‑CGC‑CAG‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGa < 2:227595/140‑1 (MQ=255) gccgcAC‑CGC‑CAT‑GATGGCGCAGGATCAGGTGTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGACAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataag > 2:423761/1‑142 (MQ=255) cAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACACTGACAATTAACCAGTTTGATAATCACGTCGTGa > 1:485393/1‑143 (MQ=255) t‑gatgGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACg > 1:109464/1‑143 (MQ=255) | TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAC‑CGC‑CAT‑GATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACG > NC_000913/3560314‑3560590 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |