Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,976,909 A→C E104A (GAG→GCG)  galR → galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,976,9090AC97.6% 121.9 / NA 42E104A (GAG→GCG) galRgalactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (25/16);  total (26/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGC  >  NC_000913/2976783‑2977048
                                                                                                                              |                                                                                                                                            
tcggtcTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAg                                                                                                                              <  2:119723/143‑1 (MQ=255)
tcggtcTGGTCGTCGGTGCCGGTTCCGCTCCGCTTTTCGGTGCAATGGTGAATGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGGTTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAg                                                                                                                              <  2:488154/143‑1 (MQ=255)
    tcTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAAc                                                                                                                          >  2:409186/1‑143 (MQ=255)
        gTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCACTGAGCAACTGAt                                                                                                                      >  2:198402/1‑143 (MQ=255)
           gTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGCTGGCTTATCACACCGGTAATTTTTTATTGATTGGCACCGGTTACCACCACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTgcaccc                                                                                                                   >  2:311719/1‑138 (MQ=255)
               gTGATGGTTCCTATCCTTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCAGTAATTTTTTATTGATAGGCAAGGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCAt                                                                                                               <  2:330050/143‑1 (MQ=255)
                tGATGTTTCGGATCCGTTTTTGGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTGATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATc                                                                                                              <  2:535895/143‑1 (MQ=255)
                   tGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAGCGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCt                                                                                                           >  2:425549/1‑143 (MQ=255)
                         cGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGCTCCGCCATCGCTGtgctg                                                                                                     >  1:130379/1‑143 (MQ=255)
                         cGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTCAATTCATTTGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAAGTGATCCGCCATCGCTGtgctg                                                                                                     >  1:123000/1‑143 (MQ=255)
                          gATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGCGTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGtgctgc                                                                                                    <  1:323541/143‑1 (MQ=255)
                            tCCGTTTTTCGGCGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGt                                                                                                  <  1:585332/143‑1 (MQ=255)
                                tttttCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGgt                                                                                              >  1:269644/1‑143 (MQ=255)
                                tttttCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCGGCGTTGgt                                                                                              >  1:283428/1‑143 (MQ=255)
                                 tgttCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTGATCACACCGGTAATTGTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGgtc                                                                                             <  2:796481/141‑1 (MQ=255)
                                       gTGCAATGGTGAAAGCGGGCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTAACACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCAGCGCTGTGCTGCGTTGGTCGTCCAt                                                                                       >  2:31791/1‑143 (MQ=255)
                                          cAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGcc                                                                                    >  1:435709/1‑143 (MQ=255)
                                                  aaaGCGGTCGAACAGGTGGCTTATCAAACCGGTAATTTGTTATGGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCACAATGAt                                                                            >  1:820817/1‑143 (MQ=255)
                                                      cGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCAGGCCAAACTGATcccc                                                                        >  2:558292/1‑142 (MQ=255)
                                                        gTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGa                                                                      <  1:425549/143‑1 (MQ=255)
                                                            aaCAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGcc                                                                  >  1:807100/1‑142 (MQ=255)
                                                              cAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGTTTACCACAACGAACAAGAAGCGCGTCAGGACATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGa                                                                <  1:345918/143‑1 (MQ=255)
                                                                      ttATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGCTGCTGATTTA‑GCCt                                                       >  1:67837/1‑143 (MQ=255)
                                                                          cacaCCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGTCAGTGTACGTTTTCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCAtt                                                   >  1:397691/1‑143 (MQ=255)
                                                                          cacaCCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCAtt                                                   <  1:409186/143‑1 (MQ=255)
                                                                           gcacCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGGACAAAAAGCGCGTCAGGCCATTGAGCAACGGATCCACCATGGCTGGGCTGCGTGGGTCGTCCATGCCGGAGGGATCACGAGTGCTGGTTTA‑GGGTCATTa                                                  >  2:697621/2‑143 (MQ=255)
                                                                           acacCGGTAATTTTTTATTGATTGGCAACGGTGACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTa                                                  <  2:546329/143‑1 (MQ=255)
                                                                            cacCGGTAATTTTTTAGTGATTGGCAACGGTTACCACAACGAACAAGAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTaa                                                 <  2:277340/143‑1 (MQ=255)
                                                                                 gTAATTTTATCTTGTGTGGCCCCGGTTACCACAACGCACAATCTGCGCGTCAGGCCATTCAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGaaa                                            <  1:30567/143‑1 (MQ=255)
                                                                                          tATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCCAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGccc                                   >  1:551008/1‑143 (MQ=255)
                                                                                          tATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑CCCTCATCAATGAAACAAATGcgc                                   >  2:314241/1‑141 (MQ=255)
                                                                                             tgattgGCAACGGTTACCACAACGAATAAAAAGCGCGTCAGGCCAGTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGt                                <  1:296186/143‑1 (MQ=255)
                                                                                               attgGCAACGGTTACCACAACGAGGAATAAGAGAAACAAGCCAATGAGCAACTGAGCCGACATCGCTCTGCAGCGTTGGTCGGCAACGCCAAAAAGATCGCGGATGC‑GAGTTACGCCACAGTAATAAAAGAAAAGCAAGGTAt                              >  2:707855/1‑143 (MQ=255)
                                                                                               attgGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGAAt                              >  2:453618/1‑143 (MQ=255)
                                                                                               attgGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAAGGAAACAAATGCCCGGtgt                              >  2:853344/1‑141 (MQ=255)
                                                                                                ttgGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCGGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGGATg                             >  2:721678/1‑143 (MQ=255)
                                                                                                 tgacAACGGTTACCACAACGAATAAAAAGCGCGTCAGGCCATTGAGCAACGGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGTATgg                            <  1:733853/140‑1 (MQ=255)
                                                                                                          ttACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTTGTCGTCCATGCCACACTGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCa                   >  2:651740/1‑143 (MQ=255)
                                                                                                             ccACAACGGCCAAAAAGCGCGTCCGGCCAGTGGGCAACGGCGCCGCCATCGCTGTGCTGTGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAAcc                <  2:425462/143‑1 (MQ=255)
                                                                                                               acaACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGt              >  1:328585/1‑143 (MQ=255)
                                                                                                                         aaaaGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATAAACCGTAGcctggctg    >  2:54640/1‑143 (MQ=255)
                                                                                                                           aaGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGc  >  1:492434/1‑143 (MQ=255)
                                                                                                                              |                                                                                                                                            
TCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGC  >  NC_000913/2976783‑2977048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑