| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 418,961 | Δ1 bp | coding (1073/1296 nt) | phoR → | sensory histidine kinase in two‑component regulatory system with PhoB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 418,960 | 0 | G | . | 97.1% | 137.6 / ‑0.4 | 35 | coding (1072/1296 nt) | phoR | sensory histidine kinase in two‑component regulatory system with PhoB |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base . (14/19); total (16/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.90e-01 | |||||||||||
TGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCACGAAA > NC_000913/418820‑419095 | tgtCTGGCAACGAATATCAGCTACGCAGTGCGTTTTCGAACCTGTTCTTTAACGCTGTGAATCATTCGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACgac < 1:328229/143‑3 (MQ=255) tGGCAACCAAGACCACCTACGCAGTGCGATTTCGAACCTCGTCTATAACGCCGTGAATCATACGCCGGACGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAC‑GACCggg < 1:828063/143‑1 (MQ=255) aCGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTCGCGTTGACGAT‑ACCGACCGGGCtttt > 1:19720/1‑142 (MQ=255) aGATCAGCTACGCAGTGCGATTTCGAACATGGTCTGTATCGCCTGGAATCAGACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCACTGCAcc > 1:863115/1‑143 (MQ=255) tCAGCTCCGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATAGCACAGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGa < 2:644241/143‑1 (MQ=255) tCAGCGACGCAGTGCGATTTCGCACCTGGTCCCGTCCCCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGa < 1:701920/143‑1 (MQ=255) gCTGCGCAGTGCGATTTCGAACCTGGTCGTTGACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAAGTTAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCa < 2:739069/143‑1 (MQ=255) gCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCa < 1:525430/143‑1 (MQ=255) tACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCATAGCACCGGAGCata > 2:698707/1‑143 (MQ=255) aGTGCGATTTCGAACCTGGTCTATAACGCAGTGAATCATCCGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCGTTGCAGCGGAGCATATGCcg > 2:647735/1‑143 (MQ=255) tGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCATATTCcgcg > 1:852038/1‑143 (MQ=255) gCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCgcgc < 1:591968/143‑1 (MQ=255) tttCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGAGCCTGa > 1:370255/1‑143 (MQ=255) tttCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGCGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCATGGCACCGGAGCATATTCCGCGCCTGa > 2:668678/1‑143 (MQ=255) aCCTGGTCTATACCGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGc < 1:647359/143‑1 (MQ=255) aCCTGGACTATAACGCCGTAAATCAGACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGc < 1:647472/143‑1 (MQ=255) tGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGGGTGCCGCACGGTGCCGAATTTAGCGTCGACGCTAACGCA‑CGGGCATTGCACCGCAGCATATTCCGCGCCTGACGGGGCGtt > 2:356947/1‑143 (MQ=255) ctgtaccgccgcGAAGCCCACGCCGGAAGGCACGCATAGCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTa < 1:72156/137‑1 (MQ=255) ataACGCCGTGAATGATACGCCGGGAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCAGGGTGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATc < 2:730198/143‑1 (MQ=255) aaTCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGGAATTATCGCGGTGATaaa > 2:262828/1‑143 (MQ=255) tCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATCAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACTGAGCGTTTGTATCGCGGTGATACAgc > 2:801526/1‑143 (MQ=255) gAAGGCACGTCTATGTCCGTACGCTGGCAGCTAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGCTAAC‑GACCGAGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCgg < 1:229719/143‑1 (MQ=255) aCGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTTCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAAcc > 1:605921/1‑143 (MQ=255) aCGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGAGCCGAATTTAGCGTTGGAGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGAGTTCCCGGCAAAcc > 1:643115/1‑143 (MQ=255) gTACGCTGGCAGCGGGTGCCGCACGGTGCCGAATTCAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGa < 1:122336/143‑1 (MQ=255) tGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCGAACCGGCGGTAGCGGAGCAggg > 2:775389/1‑143 (MQ=255) ggCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGTGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGt < 1:240367/143‑1 (MQ=255) aGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAg < 1:172909/143‑1 (MQ=255) ccGCACGGTGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTg < 2:853903/143‑1 (MQ=255) cGCACGGTGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGCTTAGGGTTAGCGGTCGTGa > 1:12566/1‑143 (MQ=255) gTGCCGAATTTAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATg < 1:555183/143‑1 (MQ=255) aaTTCAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGa < 2:554565/143‑1 (MQ=255) tttAGCGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGCGCGTTTTTATCGCGTTGATAACGCGCGTTCCCGGCACACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGCAACATGCTGTGAat > 2:893600/1‑143 (MQ=255) gCGTTGATGATCAC‑GACCGGGCATTGCACCGGAGCGTATTCCGCGCCTGGCCTAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAatcatc < 2:749943/143‑1 (MQ=255) cGTTGAAGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGAGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAAtcatca > 1:470865/1‑143 (MQ=255) aaGATAAC‑GAGCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTGTGATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCACGaaa < 1:832806/143‑1 (MQ=255) | TGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCACGAAA > NC_000913/418820‑419095 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |