| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,560,455 | 1 | . | G | 94.9% | 108.1 / ‑0.9 | 40 | intergenic (‑2/+1) | glpR/glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base G (19/18); total (21/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGA > NC_000913/3560314‑3560588 | tCGATAAACCGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGcg < 1:480665/143‑2 (MQ=255) ccAGCGCCGGGGCATTGGGGCTCTGCTCCGCGGCCTTGCGTGCGACGCGCTCTTTTGCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATc < 2:173971/142‑1 (MQ=255) gTCGGGCCATTGGGGGTGCGCTCCGCCGCTTGGCGGGCGATGCGCTCTTGTTCCTCGGGCCGGGTCGCCTTGCGATCGTGCCACGGCGTGCTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGtt < 2:861092/143‑1 (MQ=255) aTTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGATGGCCGTGCGATCGTGCCACGGCGTGTTAACCGAAGTGGAAGGCAGCGCCGCAACGCCATGAGC‑GAG‑CAGGATCAGGTCTTgagccg > 2:613849/1‑138 (MQ=255) gcccccccACGTGGCGGGCGATGCGCGCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTg < 1:305529/137‑1 (MQ=255) cGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTCGGTGGCCTTGCGATCGTGCCACGGCGTGTTTACCGGACTGGAAGGCCGCGCCGCACCGCCCTGA‑TAGAG‑AAGGTGGGGGGCTTGCCCCGCCAGCTCCTTGAGGt > 2:185644/1‑143 (MQ=255) cTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg < 1:252048/143‑1 (MQ=255) tGCGGGCTATGCGCGCTTTTGCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGa < 2:776359/143‑1 (MQ=255) gggcggCGATGCGCTCTTTTGCGTCGGTCTGGGTGGCCTGGCGATCGTGCCACGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGa < 2:284660/139‑1 (MQ=255) gCGGGCGATGCGCTCTTTTGCTTCGTTGTGGGGGGCCTTGCGATCGTGCCACGGCGTGTTAATCGAACTGGAAGGCAGCGCCGCCCCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGaa < 1:362579/143‑1 (MQ=255) cGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGACCAGGTTTTGCTCCGCCAGCTCATTGAGGCCGCGGCGAAt > 2:573679/1‑143 (MQ=255) gggCGATGCGCACTCCTCCCTCGGTCTGGGTGGCCCTGCGACCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa < 1:4524/143‑1 (MQ=255) tGCGCTCTTTTAATACTATCTGGGTGGCCTTGCGAGCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc < 1:331560/143‑1 (MQ=255) ggggcgctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc < 1:176519/137‑1 (MQ=255) ttttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCGGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGGCGCGGGGGATAGTATGCGGGCTGAc > 2:819806/1‑143 (MQ=255) ccgtcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGga < 1:429940/140‑1 (MQ=255) ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAACAGTCCGCGGGCGGACGgaga > 2:456973/1‑143 (MQ=255) gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATg > 2:299424/1‑143 (MQ=255) tCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGc > 1:383347/1‑143 (MQ=255) gggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCAGACGGAGAGATGctct > 2:871197/1‑143 (MQ=255) ggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctctc > 1:317022/1‑142 (MQ=255) cTTGCGATCGTGCCACGGCGTGTTAGCCGAACTGGAAGGCAGCGCCGCACAGCCATGA‑T‑GGCGCAGGATCAGGTATTGCTCCGCGAGCTCATTGATGTCTCTTCGAATAGTCTGCGGGCGGACGGAGAGAGGATCTAGCGGct > 1:168720/1‑143 (MQ=255) gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCTGCGCCGCACCGCCATGA‑C‑GGCGCAAGATCAGGTTTTGATCCGCCAGCCCACTGAGGTCGCGGCGAATAGTCTGCGCGCTCCCGCAGACATGCACTACCAGCTCttag > 2:423317/1‑141 (MQ=255) aTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGATATGCTCTACCCGCTCTTCgt < 2:692482/143‑2 (MQ=255) aTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCgg > 1:826552/1‑143 (MQ=255) tGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGTACCGCCATGA‑T‑GGCGCAGGATCGGGTTTGGATCCGCCATCGCCTTGCGGCCGCCGCGAAGAGTCGGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACt < 1:664492/143‑1 (MQ=255) ccACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTgc > 2:711506/1‑142 (MQ=255) tgatAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGGAGCGTCAGGTTTCGATCCGCCACCCCATTGGGGTCGCGGCCGATGGTCTGCGCGCTGACGGAGTCATGGGCGGCCTGCACTTCGGTACCGAGCTAAcaa > 2:375564/4‑141 (MQ=255) ttAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctg > 1:84317/1‑143 (MQ=255) ccGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGACGTCGCGGCCAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACAGACCTAAACctgctct > 2:225011/1‑141 (MQ=255) cGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTCATCAGTTATCCTTTTTAGGTGGTCGCCAGTGGTCTGCGTGCGGACGGAGGAAAGCGCTACCAGCTCTCCGGTACTGACCTAACCCTGCTGtg > 2:642797/1‑142 (MQ=255) aaCCGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGGCGCGGCGAGTAGTCTGCGGGCTGACGGAGAAATCCTCTACCCGCCCTTCGGTACTGGCATAACCCCGCTGgttc > 1:404943/1‑142 (MQ=255) actccaaGGCAGCGCCGCACCGCCATTA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTaa < 1:393232/138‑1 (MQ=255) tGGAAGGCAGCGCCGCACCGCCATGG‑T‑GGCGCAGGTCCCGCGACGGCGGCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc < 2:637946/143‑1 (MQ=255) gAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAACTGCTCTACCAGCTCTTCGGTCCCGACATAACCCTGCTCTTTCACCAg > 2:339105/1‑143 (MQ=255) cAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGa < 1:299424/143‑1 (MQ=255) ccgccgTGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTGTTCGGTACTGACATAACCCTGCTGCTTCACCAGTTCGATAATACCgct > 2:381683/1‑141 (MQ=255) cGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCC‑CCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCACGCTGTTTAACCAGTTCGGTAATACCGTtgt > 1:300442/1‑143 (MQ=255) cATGA‑T‑GGCGCAGGATCAGGGTCTGCACCGCCAGCTCATTGAGGTCGCGGCGAACAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGa < 1:229613/143‑1 (MQ=255) cATGA‑G‑GGCGCAGGATCGGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGa < 1:293436/143‑1 (MQ=255) cATGA‑A‑GGCACAGGCTCACGTTCTGCTCCCCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGAAACGCTCTACCAGCCCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGa < 1:230499/143‑1 (MQ=255) | TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGA > NC_000913/3560314‑3560588 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |