Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	4,001,645	Δ5 bp	coding (220‑224/951 nt)	corA →	magnesium/nickel/cobalt transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4001644	1 (0.020)	50 (1.010)	44/284	0.1	98.0%	coding (219/951 nt)	corA	magnesium/nickel/cobalt transporter
	?	NC_000913	4001650 =	1 (0.020)					coding (225/951 nt)	corA	magnesium/nickel/cobalt transporter

ATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4001510‑4001644 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTAT  >  NC_000913/4001650‑4001775                                                                                                                                                                                                                                                                       ATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCGGCATCTCCgtgt                                                                                                                        >  1:341304/1‑139     ATCTTGTCGAACCGGACGACGACGAGCGACGGAGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTGCTTTGCAGACGGCGACGGCCTGCATCTCCTgcgcctt                                                                                                                    >  2:473309/1‑136       CTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCGCGGCACTGGAAGCCATCGAAGCATCGGCACGTTTCTTTGCAGACGACGACGGCCTGCATCTCCTTCTTCTTCT                                                                                                                  >  1:600126/1‑143           TCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGA                                                                                                              <  1:627497/143‑1            CGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCATGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAA                                                                                                             <  1:757827/143‑1                 CGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGC                                                                                                        <  1:817438/143‑1                 CGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGC                                                                                                        <  1:421015/143‑1                         GACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGCCGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATC                                                                                                >  1:825045/1‑143                           CGAGCGACTGCGCGTACAAGCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCAC                                                                                              <  1:642697/143‑1                              GCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGACCTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAGGATCACGCa                                                                                           >  1:275363/1‑142                               CGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCG                                                                                          >  2:488122/1‑143                                 ACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGCAGATCACGCCGGT                                                                                        >  2:789311/1‑143                                 ACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGT                                                                                        >  1:240836/1‑143                                   TGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTGTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAg                                                                                      >  2:130419/1‑142                                     CGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCAAGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGACGATGCGGAAGATCACGCCGGTCACc                                                                                    >  1:272193/1‑142                                      ggGTACAATCTGAGCGTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCAGGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTC                                                                                   <  2:734731/141‑1                                      GCGTCCAATCAGCACTCGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTGCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTC                                                                                   <  1:770905/143‑1                                         TACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTATCTTTGAAGACGACGACCGCCTGCATCTCCTTCTTCTTCTTTGAAAATGCGGGAGATCACGCCGATAGCTCTAC                                                                                >  2:737959/1‑143                                            AATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGAACGGTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGT                                                                             >  1:258179/1‑143                                             ATCTGAACTTGGCCAGCGCATCGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTG                                                                            <  2:829926/143‑1                                             ATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTG                                                                            >  1:621054/1‑143                                              TCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGG                                                                           <  2:195689/143‑1                                              TCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGG                                                                           <  1:126351/143‑1                                              TCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACCGTGG                                                                           >  1:578875/1‑143                                              TCTGAACGTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGG                                                                           <  1:128218/143‑1                                                TGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTACTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTCACTCCACTGTGGCA                                                                         >  1:390117/1‑143                                                 GAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACCACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCAT                                                                        <  1:441019/143‑1                                                          CAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCC                                                               >  1:718156/1‑143                                                              GCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTGCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGA                                                           <  2:33776/143‑1                                                               CCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCATCTTTCTTTGTAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAT                                                          <  2:188024/143‑1                                                                CTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATG                                                         <  1:513726/143‑1                                                                 TGGCATCCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGcggg                                                        >  2:326543/1‑139                                                                  GGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGCAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTCCCATCACTGATGca                                                       >  2:31361/1‑141                                                                        CCGCCCGGAACTGGAAGACATCGAAGCATCGACACGTTTCTTTGAAGACGACGCCGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGCAACCACAATGTGGCATTTACCATCCGTGATGGTCGTATG                                                 >  1:853267/1‑143                                                                            CCGGATCTGGAAGACCTCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTA                                             <  1:303006/143‑1                                                                             CGGGACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTAC                                            <  1:555826/143‑1                                                                                 ACTGGAAGACATCGGTGCATCGGCACGTTTCTGTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCGGGTAACTCCACTGTGGCACTTACCATCCGTGATGGTCGTCTGTTTACTCTG                                        <  2:474627/143‑1                                                                                  gTGGAAGACATCGAAGCAGCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGC                                       <  1:824241/142‑1                                                                                    gcAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGT                                     <  1:635586/141‑1                                                                                     GAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGCAGATGCGGAAGCTCACGCCGGTGACTCCACTGTGGCATTTAGCAGCCGTGATGGTCGTCGGTTTACTCTGCGcg                                    >  1:296441/1‑141                                                                                     GAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTACCTCCACTGTGTCATTTCCCATCCGTGATGGTCGTCTGTTTACTGTGCGTG                                    >  1:588684/1‑143                                                                                          cagCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGT                               <  1:460223/140‑1                                                                                           ATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTG                              >  1:490355/1‑143                                                                                             cgacgtATCGGGAGGTTTCTTTGACGACGGCGACGGCCTGCATCGCCTGCTTCTTCTTGGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCAGTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAA                            <  2:349218/137‑1                                                                                                           GTTTCTTTGAAGGCGGCGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGAGCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCG              <  2:560606/143‑1                                                                                                           GTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTGGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCCTTTACCATCCGTGATGGTCGTCGGTGTACTCTGCGTGCGCGTGAACGCGCCGCTTgtgc              >  2:702249/1‑139                                                                                                            tgtcgtgGAAGACGACGACGGCATGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGT             <  1:279957/136‑1                                                                                                                TTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGGGAACTGCCCGCTTGTCGCCTGT         >  2:668712/1‑143                                                                                                                    AAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTGGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCG     >  1:733065/1‑143                                                                                                                       tCGACGACGCTCTGCATGTTCGTCTTCTTCTTTGTAGATGCGGCAGATCACGCCGGTAATTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTATTCTGTGTGTGCGTGAACTGCCCGCTTTTCGTCTGTATCGTAT  <  2:550741/142‑1                                                                                                                                                                                                                                                                       ATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4001510‑4001644 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTAT  >  NC_000913/4001650‑4001775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.