| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,560,455 | 1 | . | G | 97.4% | 111.3 / ‑3.2 | 38 | intergenic (‑2/+1) | glpR/glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (16/21); total (16/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01 | |||||||||||
ATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGT > NC_000913/3560313‑3560591 | atCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGc < 1:619592/143‑1 (MQ=255) aGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTGGCGATGGTGGCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGGTCAg > 1:620692/1‑143 (MQ=255) cGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGttt‑t > 1:876719/1‑143 (MQ=255) gAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑Tg < 1:153555/143‑1 (MQ=255) gAGCCATTGGCGATTTGCTCCGCCACGTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑Tg < 2:398818/143‑1 (MQ=255) gCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTCTTCTTCGGT‑CTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TGCt < 1:873322/143‑1 (MQ=255) ccATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTc < 1:92706/143‑1 (MQ=255) aTTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑CGCTCCg > 2:829902/1‑143 (MQ=255) ttcgggATGTGTTCTGCCAGTTTGCGGGCGATGTGCTCTTGTTCTTCGGT‑CTGGGTGGCCTTGCGATCGTGTCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGc < 2:898096/140‑1 (MQ=255) ggctttGCTCCGCCACCGTGCCGGCTAGTCGCTCTTTTTATAGGGG‑CTTGGCGGCCTTGCGATCGTGCCACGCCGTTTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCAGc < 1:923273/140‑1 (MQ=255) ggATTGGCTCGGGCACCGGGCGGGCGATGCGCTCTGTTTCTTCGGTCCTAGGACACTCTGC‑ATCTGGCCACGGCGTGTTAACCGAACTGGAAGTCAGCGCCGCACGGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCAGc < 1:927167/143‑1 (MQ=255) tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAg > 1:334438/1‑143 (MQ=255) tttGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGATGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TCCTCCGCCAGCTCATTGAGGTCgcggc > 2:226889/1‑143 (MQ=255) gCGGGCGATGCGCTCTTGTTCGTCGGT‑CTGGGTGGCCTGGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGaa < 2:737914/143‑1 (MQ=255) tGCGCTCTTTTTCTGCGGT‑GTGGGTGGCCTCGCGTTCGCGCGACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc < 1:880963/143‑1 (MQ=255) cgcTCTTGTTCTTCGGT‑CTGGCTTGCCTTGCGATCGGGCCTCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg < 2:297983/143‑1 (MQ=255) tctTTTTCTTCGGT‑CTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCt > 1:442906/1‑143 (MQ=255) tttttCTTCGGT‑CTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGGCTGCGGGCCGa > 2:259236/1‑143 (MQ=255) ggT‑CTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaagt > 1:746199/1‑141 (MQ=255) ggT‑CTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAt < 2:876719/143‑1 (MQ=255) gcgttGTGATCGTGGCACGGTGTGTTAGCCGAACTGGACGGCAGCGCCGGACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCAGCTCATGGAGGTCGCGGCGAATAGGCTGCGGGCTGTCGGAGAAATGGTCTACCAg < 2:296702/140‑1 (MQ=255) ggtggggCCACGGCGTGTTAACCGAACGGGAGGGCAGCGCCGCGCCGCCATGACGGCGCAGTTTGATGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg < 2:558885/137‑1 (MQ=255) aTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCGCCCCATCGAGGACGGGCCGCATAGTCTGCGGGCTGACGGAGAAATGCTCGACCCGCCCTTCgg > 2:653101/1‑143 (MQ=255) aTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCGGACGGAGAAATGCTCTACCAGCTCTTCgg > 1:615994/1‑143 (MQ=255) tGCCACGGCTTGTTAACCGTACTCGTCGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACt < 2:405421/143‑1 (MQ=255) gCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCCGCCCTTCGGTACTg > 1:711194/1‑143 (MQ=255) ccACCGGGTGGTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAGCAC‑GCCGCTTAAATCTGTGCTCCGCAAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa < 1:679737/143‑1 (MQ=255) aCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACGGCCATGATGGCGCAGGGTCAGGTTT‑TACTCCGCCAGCTCATTGAGGTCGCGGCGAATCGGCTGAGGGCTGACGGAGAAAGGCGCGAGCAGCTCTTCGGTACTGAcg > 2:189957/1‑142 (MQ=255) gtgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAcc > 1:84256/1‑143 (MQ=255) tAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgc > 1:81020/1‑143 (MQ=255) cGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGtt < 1:603426/143‑1 (MQ=255) cTGTAAGGCTGCGCCGCACCGCCATGATGGCGCGGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc < 2:265261/143‑1 (MQ=255) aGGCAGCGCGGCACCGCCATGACGGCGCAGGATCAGGTTT‑GGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt < 1:315185/143‑1 (MQ=255) aGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGTTTT‑TTCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt < 1:388580/143‑1 (MQ=255) aGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt < 2:334438/143‑1 (MQ=255) ccgcACCGCCATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataata < 1:734867/143‑1 (MQ=255) cATGATGGCGCAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGa < 1:934407/143‑1 (MQ=255) tgatgGCGCAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACg > 1:599826/1‑143 (MQ=255) gatgGCGCAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCACCTCTCCGGTACTGACATAACCCAGCTGCTTAACCCGTTCGATAATACCCGCGTGACgg > 2:925196/1‑142 (MQ=255) | ATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTT‑TGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGT > NC_000913/3560313‑3560591 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |