Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	intergenic (‑271/‑264)	insA ← / → uspC	IS1 repressor TnpA/universal stress protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	71 (1.400)	53/282	0.0	100%	intergenic (‑271/‑267)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	64 (1.260)	38/282	0.3	100%	intergenic (‑274/‑264)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

AAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGA  >  NC_000913/2289972‑2290255                                                                                                                                              |                                                                                                                                               aaGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCCCATATCAGGCACTGGTTCGCAGCTTCCa                                                                                                                                               >  2:688905/1‑143 (MQ=2)   gAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCAACTTCCATa                                                                                                                                             >  1:542538/1‑143 (MQ=17)       aTCTCCTTTCTGGTCTGACGGCGCTTACTGCTGAAGTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGc                                                                                                                                         <  2:738175/143‑1 (MQ=25)           ctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACACACATGATCTCCTCACAGCGACTTGGTCCCACCCTCCATAACGCTGTa                                                                                                                                     >  1:663309/1‑143 (MQ=11)           ctTGTCTGGTCTGCCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATTACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGTGACTTGTTCGCACCTTCCATAACGCTGTa                                                                                                                                     <  2:700933/143‑1 (MQ=255)            ttttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTACGTTGATGACTCATGATGAACCCTGATCTATGGCTCCAGATGACAAACATGATCTCCTATCCGGCAGTTGTTAGCACCTTACACACCGCTGtcg                                                                                                                                    >  2:545757/1‑141 (MQ=11)             tttGTGGTATGACGGCGGTTACTGCGGAATTCACTGTCGGCGGAGGTGAGTTGATGACTCATGATGAGCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGc                                                                                                                                   <  2:860229/143‑1 (MQ=14)             tttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGc                                                                                                                                   >  1:842698/1‑143 (MQ=39)                ccggTCTGCCGGCGCTTACTCCTGACTTCACTTGCGGCGAAGGTAAGTTGATGACTCATGATGACCCCTGTTCTATGGCTCCAGATGACATACATGATGTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGccac                                                                                                                                <  2:649321/141‑1 (MQ=14)                  ggTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCCTCCATAACGCTGTAGccacaa                                                                                                                              >  1:718057/1‑141 (MQ=255)                   gTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCTGGGCCTTGTTCGGACCTTCCATAACGCTATAGCAACCAg                                                                                                                             >  1:121492/1‑143 (MQ=14)                   gTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGAATTGTTCGCACCTTACATACCGCTGTAGCAACGAg                                                                                                                             >  2:833113/1‑143 (MQ=11)                      tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAAc                                                                                                                          <  1:911730/143‑1 (MQ=255)                                   tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAACTCCGATCTCATATAAGAGACTTGCTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATCTTGCGGac                                                                                                             >  2:908176/1‑142 (MQ=17)                                          ttCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTACTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACaaa                                                                                                      <  1:677595/143‑1 (MQ=255)                                             aCTGTCGGCGAAGGTAAGTTGATGACTAATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAagag                                                                                                   >  1:924518/1‑143 (MQ=255)                                                    gCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGa                                                                                            >  1:933147/1‑143 (MQ=255)                                                            aaGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATCTCAGGGACGTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATACTACGCCACGACAAACAGGAACACAACCAGAtg                                                                                    >  2:850799/1‑142 (MQ=18)                                                             aGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACATAACGCTGTAGCCACCAGAACAGATATTGCGGAACTACAAAGAGAAACAGAACCAGATTg                                                                                   >  1:469847/1‑143 (MQ=255)                                                                   tgaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCAt                                                                             <  1:460997/143‑1 (MQ=255)                                                                          tgttgaTCCGTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCGGTAGCCACCAGAACATATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCtt                                                                      <  1:627975/140‑1 (MQ=37)                                                                             cgaACCCTGTGCGATGGCTGCAGATGACAAACATGATCTCATATCAGGGACGTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCa                                                                   <  1:16383/142‑1 (MQ=255)                                                                               aaCCCTGTTCTATGGCTCCCGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATc                                                                 <  1:457187/143‑1 (MQ=255)                                                                               aaCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGACCGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATc                                                                 <  1:456647/143‑1 (MQ=255)                                                                                aCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATcc                                                                >  2:833388/1‑143 (MQ=255)                                                                                  ccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTa                                                              >  1:587729/1‑143 (MQ=255)                                                                                  ccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTa                                                              >  1:672883/1‑143 (MQ=255)                                                                                  ccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTa                                                              >  1:117056/1‑143 (MQ=255)                                                                                  ccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTa                                                              >  1:759511/1‑143 (MQ=255)                                                                                         tATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGGACGACACAGAGAACCAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAaatt                                                       >  1:443477/1‑143 (MQ=255)                                                                                         tATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGACAACATAACTTATATTACGGATGTACAAGTAAGAACAGAACCAGATTGATGCATTAAGCTTTCATCCTATGAaatt                                                       >  2:660789/1‑143 (MQ=21)                                                                                          aTGGCTCCACAGGCAAAACATGATCCTATACCAAGGACTCGTTCGCACCTTCCAGAACGCTGTAGCCACCAGAACAGATATTGCGGAACTACAAAGAGACACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAaatta                                                      <  2:589332/143‑1 (MQ=255)                                                                                            ggCTCCAGATGACAAACATGACCTCATATCAGGGACTTGTGCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAaattaat                                                    <  2:706400/143‑1 (MQ=255)                                                                                             gCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAaattaatt                                                   >  1:810963/1‑143 (MQ=255)                                                                                               tttaGATGATAAACATGATCTTATATCAGTGATTTGTTTTCACCTTCTATAACGCTGTAGTCATCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGTATTGAGCTTTCATCCTATGAAATTAATTGc                                                 <  2:542538/140‑1 (MQ=255)                                                                                                     tGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACATAACGCTGTAGCCACCAGAACAGATATTGAGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTGTCATCCTATGAAATTAATTGCTgacaa                                           >  2:392598/1‑139 (MQ=255)                                                                                                       aCAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTaaaa                                         >  1:562842/1‑143 (MQ=255)                                                                                                         aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACAAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGTTGTTACAAGc                                       >  2:839729/1‑143 (MQ=255)                                                                                                           aCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCAt                                     >  1:792947/1‑143 (MQ=255)                                                                                                            cATGATCTCAGATCAGGGGCTTGTACGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCAtt                                    <  1:804241/143‑1 (MQ=255)                                                                                                                  ctcctttCAGGGACTTCTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAAAGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTAc                              <  2:532350/137‑1 (MQ=255)                                                                                                                  ctcATATCACGGAGTGCTTCGCACCTTCCATAACGCTGTAGCCACTAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTAc                              <  2:842698/143‑1 (MQ=255)                                                                                                                     atatCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAAACAGATTGATGCAGTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGa                           >  1:780380/1‑143 (MQ=255)                                                                                                                         cAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAAt                       >  1:765211/1‑143 (MQ=255)                                                                                                                              aCTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGCTGCATTGAGCTTTCATCCTATGAACTTAATTGCTGTTAAAAGCATTCGGTACAGAAAATACCCa                  >  1:622569/1‑143 (MQ=255)                                                                                                                              aCTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCa                  <  2:515676/143‑1 (MQ=255)                                                                                                                                ttGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACCAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCCTCCTATGAACTTAATTGATGGTAAAAGTGTTGGGTACAGAAAATACGCATa                >  1:70687/1‑143 (MQ=255)                                                                                                                                 tGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACCGTAAATACCCATAg               >  1:123816/1‑143 (MQ=255)                                                                                                                                     cGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTcc           <  2:718057/143‑1 (MQ=255)                                                                                                                                     cGCACCTTCCAAAACGCTGTAGGCACCAGAACAGCTATTGCGGCACGACAAAGAGAAACAGAACCAGAGTCATGCATTCAGCCTTCATGCGATGAAACTAATTGCTCTTAAAAGCCTTGGGTCTAGCCAAGATCCATAGCtgc           >  2:710801/1‑141 (MQ=255)                                                                                                                                           ttCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATAccc     >  1:787899/1‑143 (MQ=255)                                                                                                                                              cATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAATATCGCAGAGGTCCATACCCGGa  >  2:694345/1‑143 (MQ=255)                                                                                                                                              |                                                                                                                                               AAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGA  >  NC_000913/2289972‑2290255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.