Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 451,643 G→A 76.7% intergenic (‑33/+427) cyoA ← / ← ampG cytochrome o ubiquinol oxidase subunit II/muropeptide transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913451,6430GA76.7% 127.6 / 31.7 61intergenic (‑33/+427)cyoA/ampGcytochrome o ubiquinol oxidase subunit II/muropeptide transporter
Reads supporting (aligned to +/- strand):  ref base G (7/7);  new base A (17/29);  total (25/36)
Fisher's exact test for biased strand distribution p-value = 5.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGACCAGTTCGGGCTGTACTTAGCATCTTTATTGCTCGCACGGTACTTCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGACAACTGGGTGATGAGAAAATAAAGA  >  NC_000913/451362‑451906
                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                        
tgACCAGTTCGGGCTGTACTTAGCATCTTTATTGCTCGCACGGTACTTCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAg                                                                                                                                                                                                                                                                   >  1:258054/1‑289 (MQ=255)
tgACCAGTTCGGGCTGTACTTAGCATCTTTATTGCTCGCACGGTACTTCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAg                                                                                                                                                                                                                                                                   >  1:341635/1‑289 (MQ=255)
      gTTCGGGCTGTACTTAGCATCTTTATTGCTCGCACGGTACTTCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGtgtgtg                                                                                                                                                                                                                                                             >  2:581279/1‑289 (MQ=255)
       ttCGGGCTGTACTTAGCATCTTTATTGCTCGCACGGTACTTCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTgg                                                                                                                                                                                                                                                            >  2:596202/1‑289 (MQ=255)
        tCGGGCTGTACTTAGCATCTTTATTGCTCGCACGGTACTTCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTggg                                                                                                                                                                                                                                                           <  2:250347/289‑1 (MQ=255)
                tACTTAGCATCTTTATTGCTCGCACGGTACTTCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATAAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTTAGTCTCATTTAACGACCTCAATTCACCGGGATTTGGTGGCGTTTAAAGTGTGTGGGATTTTTAc                                                                                                                                                                                                                                                   >  2:659419/1‑289 (MQ=255)
                 aCTTAGCATCTTTATTGCTCGCACGGTACTTCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATTGGGTTGCGTTTAAAGTTCTTGGGCCTTTTACg                                                                                                                                                                                                                                                  >  2:373494/1‑289 (MQ=255)
                   ttAGCATCTTTATTGCTCGCACGGTACTTCCAGGCGAAACCAACAGCCATCAAGATTGCGTGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACggg                                                                                                                                                                                                                                                <  2:341635/289‑1 (MQ=255)
                   ttAGCATCTTTATTGCTCGCACGGTACTTCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACggg                                                                                                                                                                                                                                                <  1:590763/289‑1 (MQ=255)
                     aGCATCTTTATTGCTCGCACGGTACTTCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGaa                                                                                                                                                                                                                                              <  1:422239/289‑1 (MQ=255)
                        aTCTTTATTGCTCGCACGGTACTTCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAgg                                                                                                                                                                                                                                            <  2:229600/288‑1 (MQ=255)
                             tATTGCTCGCACGGTACTTCCAGGCGAAACCAAAAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACAt                                                                                                                                                                                                                                      <  2:283912/289‑1 (MQ=255)
                                    cGCACGGTACTTCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGTAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCATGGGATCTGGTGGCTTTTAACGTGTGTGGGCATTTTACGGGAAGGTTACATTTATGTa                                                                                                                                                                                                                               >  2:297234/1‑289 (MQ=255)
                                    cGCACGGTACTTCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTa                                                                                                                                                                                                                               >  1:533482/1‑289 (MQ=255)
                                        cGGTACTTCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACa                                                                                                                                                                                                                           <  1:171041/289‑1 (MQ=255)
                                        cGGTACTTCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACa                                                                                                                                                                                                                           <  2:496851/289‑1 (MQ=255)
                                        cGGTACTTCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACa                                                                                                                                                                                                                           <  1:186269/289‑1 (MQ=255)
                                                   ggCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAAtt                                                                                                                                                                                                                 >  2:206787/1‑288 (MQ=255)
                                                     cGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGa                                                                                                                                                                                                                        <  1:106257/279‑1 (MQ=255)
                                                     cGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGa                                                                                                                                                                                                                        >  2:106257/1‑279 (MQ=255)
                                                                    tCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTg                                                                                                                                                                                                >  1:673796/1‑288 (MQ=255)
                                                                      aaGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCt                                                                                                                                                                                             <  2:406160/289‑1 (MQ=255)
                                                                          ttGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGcc                                                                                                                                                                                          <  2:513432/288‑1 (MQ=255)
                                                                          ttGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGcc                                                                                                                                                                                          <  2:250827/288‑1 (MQ=255)
                                                                          ttGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCa                                                                                                                                                                                         <  2:509469/289‑1 (MQ=255)
                                                                                   tAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTAAGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACata                                                                                                                                                                                <  1:596202/289‑1 (MQ=255)
                                                                                        aCAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTg                                                                                                                                                                           <  2:258054/289‑1 (MQ=255)
                                                                                         cAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTg                                                                                                                                                                           <  1:206787/288‑1 (MQ=255)
                                                                                                                                          aCCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATaa                                                                                                                         <  2:73147/289‑1 (MQ=255)
                                                                                                                                           ccAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTtat                                                                                                                           <  1:269649/286‑1 (MQ=255)
                                                                                                                                                             tCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAg                                                                                                     >  2:71337/1‑289 (MQ=255)
                                                                                                                                                                       cAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTgggc                                                                                           >  1:225831/1‑289 (MQ=255)
                                                                                                                                                                        aGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTgggcg                                                                                          <  2:225831/289‑1 (MQ=255)
                                                                                                                                                                        aGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTgggc                                                                                           <  1:373494/288‑1 (MQ=255)
                                                                                                                                                                           aTTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTgggcg                                                                                          <  1:581279/286‑1 (MQ=255)
                                                                                                                                                                            ttACAGCCACGAGCAAATACAGTCCTGGCAAATAATAAAAACCATCCCAAACTTTTATGTTATTTCCTGATTCTCATTTAACGACCTCAATTCCCCGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGAAAGTTTACTTTACTGTAAAAATGTTTAATTTCCTGTTACTTGGTTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCaa                                                                                     <  2:475743/289‑1 (MQ=255)
                                                                                                                                                                             tACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCaa                                                                                     <  1:248767/289‑1 (MQ=255)
                                                                                                                                                                             tACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCaa                                                                                     <  1:297234/289‑1 (MQ=255)
                                                                                                                                                                               cAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTtat                                                                                                                           >  2:174785/1‑251 (MQ=255)
                                                                                                                                                                               cAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTtat                                                                                                                           <  1:174785/251‑1 (MQ=255)
                                                                                                                                                                                    aCTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGtt                                                                                                                             <  2:285324/243‑1 (MQ=255)
                                                                                                                                                                                    aCTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGtt                                                                                                                             >  1:285324/1‑243 (MQ=255)
                                                                                                                                                                                      tGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCGCCCGGTTAACTATTTTTCAAAACATTACAGGTTGGTTTTAAAGAGCTGTTTCAAAAAGGCGTTATAAAAAAAGGGGAAGTGTGAAGGAATTCGGGGGGGGCATTTGTATaa                                                                            >  1:475743/1‑288 (MQ=255)
                                                                                                                                                                                                   tGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGcc                                                                                                                                                                                          <  1:719613/168‑1 (MQ=255)
                                                                                                                                                                                                   tGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGcc                                                                                                                                                                                          >  2:719613/1‑168 (MQ=255)
                                                                                                                                                                                                        gCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGAAACACATATCAGGTTGATTTTCAGAATCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGATTCTGGGCGGGCAATTGGTGTAAACAATGTGAAATaacaa                                                           >  2:280285/1‑286 (MQ=255)
                                                                                                                                                                                                             taatGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCaa                                                                                     >  1:590420/1‑258 (MQ=255)
                                                                                                                                                                                                             taatGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCaa                                                                                     <  2:590420/258‑1 (MQ=255)
                                                                                                                                                                                                                   cAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAAc                                                >  1:539120/1‑289 (MQ=255)
                                                                                                                                                                                                                      ccATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACaaa                                             <  1:71337/289‑1 (MQ=255)
                                                                                                                                                                                                                          cccAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAaataat                                         >  2:297815/1‑289 (MQ=255)
                                                                                                                                                                                                                                 ttttATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCAtt                                  <  2:539120/289‑1 (MQ=255)
                                                                                                                                                                                                                                     aTTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTagag                              <  1:659419/289‑1 (MQ=255)
                                                                                                                                                                                                                                           tttCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTggg                                                                                            <  2:261611/221‑1 (MQ=255)
                                                                                                                                                                                                                                           tttCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTggg                                                                                            >  1:261611/1‑221 (MQ=255)
                                                                                                                                                                                                                                                   gTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCaa                                                                                     <  1:175998/220‑1 (MQ=255)
                                                                                                                                                                                                                                                   gTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCaa                                                                                     >  2:175998/1‑220 (MQ=255)
                                                                                                                                                                                                                                                   gTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGACAACTGGGtga                >  1:37048/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                 ccTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTTGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGACAACTGGGTGATGAGAAAATAAAGa  <  2:533482/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                  cTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTtat                                                                                                                           >  1:325783/1‑168 (MQ=255)
                                                                                                                                                                                                                                                                  cTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTtat                                                                                                                           <  2:325783/168‑1 (MQ=255)
                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                        
TGACCAGTTCGGGCTGTACTTAGCATCTTTATTGCTCGCACGGTACTTCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGACAACTGGGTGATGAGAAAATAAAGA  >  NC_000913/451362‑451906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: