Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 970,421 C→T 4.9% R345C (CGC→TGC)  ycaQ → DUF1006 family protein with C‑terminal wHTH domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913970,4210CT 4.9% 283.3 / 3.5 82R345C (CGC→TGC) ycaQDUF1006 family protein with C‑terminal wHTH domain
Reads supporting (aligned to +/- strand):  ref base C (34/44);  new base T (2/2);  total (36/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

AAATCATTGCTGTGCATGTTGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCC  >  NC_000913/970137‑970706
                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                             
aaaTCATTGCTGTGCATGTTGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGc                                                                                                                                                                                                                                                                                             <  1:265580/287‑1 (MQ=255)
      ttGCTGTGCATGTTGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTAATTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGGTATTTTGTTATGCCGTTATTACATCGTGGGCAATTAGTCGGGCGAATGGTTGCCAAAATGCATCGCAAGAccg                                                                                                                                                                                                                                                                                      >  2:148088/1‑286 (MQ=255)
       tGCTGTGCATGTTGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCCCCAGACAGGc                                                                                                                                                                                                                                                                                    >  2:351717/1‑289 (MQ=255)
            tgCATGTTGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATcc                                                                                                                                                                                                                                                                                <  2:159624/288‑1 (MQ=255)
             gCATGTTGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGGGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCtt                                                                                                                                                                                                                                                                              <  2:710752/289‑1 (MQ=255)
             gCATGTTGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCtt                                                                                                                                                                                                                                                                              <  1:136813/289‑1 (MQ=255)
              cATGTTGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCtt                                                                                                                                                                                                                                                                              <  2:47607/288‑1 (MQ=255)
              cATGTTGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTg                                                                                                                                                                                                                                                                             >  1:67041/1‑289 (MQ=255)
              cATGTTGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTg                                                                                                                                                                                                                                                                             >  2:75367/1‑289 (MQ=255)
              cATGTGGAAAAATTGGGCAACCTTGGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCtt                                                                                                                                                                                                                                                                              <  2:367956/288‑1 (MQ=255)
                 gTTGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAg                                                                                                                                                                                                                                                                          >  1:467784/1‑289 (MQ=255)
                 gTTGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAg                                                                                                                                                                                                                                                                          >  1:333522/1‑289 (MQ=255)
                   tGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGtt                                                                                                                                                                                                                                                                        >  2:349563/1‑289 (MQ=255)
                    gAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATCTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATTCATCCCCATACAGGCATCCTTGACGTTa                                                                                                                                                                                                                                                                       >  1:422587/1‑289 (MQ=255)
                           tGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATctctct                                                                                                                                                                                                                                                                <  1:454657/289‑1 (MQ=255)
                           tGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATctctct                                                                                                                                                                                                                                                                <  1:149862/289‑1 (MQ=255)
                                       ggctcgaggttgatTTGCGGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTTCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGa                                                                                                                                                                                                                                                    <  2:113104/279‑1 (MQ=255)
                                       ggCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCCATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGa                                                                                                                                                                                                                                                    <  1:224240/289‑1 (MQ=255)
                                         cTGCAGGATGATTTGATGCCGCTACTCGAGCGGGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACATTCGCCTTTTGAGCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCACCAGACAGGCATCCCTGAAGTTATCTCTCTGTGGTTACAGGaa                                                                                                                                                                                                                                                   <  2:702132/288‑1 (MQ=255)
                                            cATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCtt                                                                                                                                                                                                                                                                              <  1:83331/258‑1 (MQ=255)
                                            cATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCtt                                                                                                                                                                                                                                                                              >  2:83331/1‑258 (MQ=255)
                                                      tgctgcCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAAc                                                                                                                                                                                                                                     >  2:113434/1‑289 (MQ=255)
                                                          gcCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACa                                                                                                                                                                                                                                 <  1:580181/289‑1 (MQ=255)
                                                               tACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGc                                                                                                                                                                                                                             <  1:75367/288‑1 (MQ=255)
                                                                     agcgggcgCTGGCCGGAAAGCTACCTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCaaa                                                                                                                                                                                                                       <  2:186503/288‑1 (MQ=255)
                                                                           cgcTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGc                                                                                                                                                                                                                 <  2:67041/288‑1 (MQ=255)
                                                                            ccTGGCCGAAAAGGTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGc                                                                                                                                                                                                                 <  2:716009/286‑1 (MQ=255)
                                                                                                   cGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTc                                                                                                                                                                                          >  2:37739/1‑287 (MQ=255)
                                                                                                        aGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTa                                                                                                                                                                                      >  1:200186/1‑286 (MQ=255)
                                                                                                          cgcgGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTg                                                                                                                                                                                  >  1:65205/1‑288 (MQ=255)
                                                                                                                    tCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGgca                                                                                                                                                                               <  2:691339/281‑1 (MQ=255)
                                                                                                                    tCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGgca                                                                                                                                                                               >  1:691339/1‑281 (MQ=255)
                                                                                                                         ttttGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCgg                                                                                                                                                                  >  2:147283/1‑289 (MQ=255)
                                                                                                                         ttttGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCgg                                                                                                                                                                  >  2:667793/1‑289 (MQ=255)
                                                                                                                          tttGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCggg                                                                                                                                                                 <  2:613700/289‑1 (MQ=255)
                                                                                                                           ttGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCaaa                                                                                                                                                                                                                       >  1:67270/1‑234 (MQ=255)
                                                                                                                           ttGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCaaa                                                                                                                                                                                                                       <  2:67270/234‑1 (MQ=255)
                                                                                                                           ttGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGt                                                                                                                                                                <  1:349563/289‑1 (MQ=255)
                                                                                                                           tgggtCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGt                                                                                                                                                                <  1:159606/285‑1 (MQ=255)
                                                                                                                           gtGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCGTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTGCGCTAACTGGCAGCAGGCAACGCGGGt                                                                                                                                                                <  1:84318/288‑1 (MQ=255)
                                                                                                                                         tGGGATCGCAAACGCGCAGGGCAGCTTTTGGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGAtgct                                                                                                                                                  <  1:586564/289‑1 (MQ=255)
                                                                                                                                                   aaCGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAgg                                                                                                                                        <  2:218357/289‑1 (MQ=255)
                                                                                                                                                    aCGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGc                                                                                                                                       <  1:351717/289‑1 (MQ=255)
                                                                                                                                                    aCGCGCAGAGCAGCTTTTTGAGTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGc                                                                                                                                       <  2:291558/289‑1 (MQ=255)
                                                                                                                                                              cagcTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACt                                                                                                                              <  2:65205/288‑1 (MQ=255)
                                                                                                                                                                 cTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTAAGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAATGCTCCTCTACTGAtt                                                                                                                          >  2:229410/1‑289 (MQ=255)
                                                                                                                                                                           ttAGCTCCCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTTCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCgg                                                                                                                <  2:166247/289‑1 (MQ=255)
                                                                                                                                                                           ttAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCgg                                                                                                                <  1:147283/289‑1 (MQ=255)
                                                                                                                                                                                tACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTggg                                                                                                           <  1:667793/289‑1 (MQ=255)
                                                                                                                                                                                             gCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGt                                                                                              <  1:37739/289‑1 (MQ=255)
                                                                                                                                                                                             gCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGt                                                                                              <  1:196658/289‑1 (MQ=255)
                                                                                                                                                                                                      cAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATTGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAAt                                                                                     >  2:397202/1‑289 (MQ=255)
                                                                                                                                                                                                      cAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATTGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAAt                                                                                     >  2:397198/1‑289 (MQ=255)
                                                                                                                                                                                                         cgcCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGa                                                                                                                                                                                                                                                    <  1:78818/127‑1 (MQ=255)
                                                                                                                                                                                                         cgcCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGa                                                                                                                                                                                                                                                    >  2:78818/1‑127 (MQ=255)
                                                                                                                                                                                                         cgcCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCAGCGGCTGGGAAATAGACCCCGTCGCCTAAATGAt                                                                                  >  1:607930/1‑289 (MQ=255)
                                                                                                                                                                                                         cgcCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATCTCCGCACCGGCTGGGACATAGACCCCGTCGCATaaagtat                                                                                  >  2:444791/1‑285 (MQ=255)
                                                                                                                                                                                                           ccGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGt                                                                                                                                                                >  1:576146/1‑209 (MQ=255)
                                                                                                                                                                                                           ccGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGt                                                                                                                                                                <  2:576146/209‑1 (MQ=255)
                                                                                                                                                                                                              aaaCGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGCTTTCGCTAAATGGCAGCAGGCAACGCGGGTGACCTTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGGAATGGAACGCGTACCATCAATGCTCATGa                                                                             >  2:40371/1‑289 (MQ=255)
                                                                                                                                                                                                                         tGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATg                                                                  <  1:282714/289‑1 (MQ=255)
                                                                                                                                                                                                                         tGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATg                                                                  <  2:467784/289‑1 (MQ=255)
                                                                                                                                                                                                                               ttttGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATATATGATTATGATAAGCTTTATGCATTCa                                                            >  1:513475/1‑289 (MQ=255)
                                                                                                                                                                                                                                         ccGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGa                                                                                                                                                              <  1:619604/181‑1 (MQ=255)
                                                                                                                                                                                                                                         ccGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGa                                                                                                                                                              >  2:619604/1‑181 (MQ=255)
                                                                                                                                                                                                                                              attaCATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGAt                                                                            <  1:605780/258‑1 (MQ=255)
                                                                                                                                                                                                                                              attaCATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGAt                                                                            >  2:605780/1‑258 (MQ=255)
                                                                                                                                                                                                                                                  cATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTggag                                         <  1:113434/289‑1 (MQ=255)
                                                                                                                                                                                                                                                   aTCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTggagg                                        >  1:435768/1‑289 (MQ=255)
                                                                                                                                                                                                                                                     cGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTggagga                                       >  1:332160/1‑288 (MQ=255)
                                                                                                                                                                                                                                                       tGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACt                                    >  1:616929/1‑289 (MQ=255)
                                                                                                                                                                                                                                                       tGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAAAGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAACTAGCCCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGACCCATCTGGAGGAACt                                    >  2:339472/1‑289 (MQ=255)
                                                                                                                                                                                                                                                         ggCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTAt                                  >  1:411934/1‑289 (MQ=255)
                                                                                                                                                                                                                                                              ttAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGaa                                      <  1:264685/280‑1 (MQ=255)
                                                                                                                                                                                                                                                              ttAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGaa                                      >  2:264685/1‑280 (MQ=255)
                                                                                                                                                                                                                                                                   tGGGCGAATGGATGCCAAAATGCATTGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGt                        <  1:397202/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tGGGCGAATGGATGCCAAAATGCATTGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGt                        <  1:397198/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                    gggCGAATGGATGCCAAAATGCATCGCGAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTc                       <  2:92993/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                    gggCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATCGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCg                         >  2:645339/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                                          aTCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGc                                                                                                                                       >  2:708759/1‑155 (MQ=255)
                                                                                                                                                                                                                                                                                          aTCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGc                                                                                                                                       <  1:708759/155‑1 (MQ=255)
                                                                                                                                                                                                                                                                                          aTCGCCAGACAGGCATCCGTGAAGTTATCTCTCTGTGGTTACAGGAAGGTAGTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATtgcctgcc  <  1:40371/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                             
AAATCATTGCTGTGCATGTTGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGATCGCAAACGCGCAGAGCAGCTTTTTGATTTTAGCTACCGGCTGGAGTGCTATACCCCAGCGCCGAAACGCCAGTATGGCTATTTTGTTCTGCCGTTATTACATCGTGGGCAATTAGTTGGGCGAATGGATGCCAAAATGCATCGCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCC  >  NC_000913/970137‑970706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: