| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,951,535 | Δ1 bp | pseudogene (977/984 nt) | ilvG → | pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive, acetolactate synthase II, large subunit, cryptic, interrupted |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,951,533 | 0 | A | . | 98.0% | 205.6 / ‑1.7 | 50 | pseudogene (975/984 nt) | ilvG | pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive, acetolactate synthase II, large subunit, cryptic, interrupted |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (31/17); minor base G (0/1); total (32/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTG > NC_000913/3951392‑3951669 | cGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTaa > 1:691383/1‑143 (MQ=255) ggCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGAGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTaat > 1:712217/1‑142 (MQ=255) accgacccccTTCGCGCCACACGCCATTGTTCTCCATATCGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATTCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATg < 1:352046/135‑1 (MQ=255) tGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGAc > 1:698944/1‑143 (MQ=255) acacCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTgg > 1:526177/1‑143 (MQ=255) acCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGgca > 1:28412/1‑143 (MQ=255) ttCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGCCAGCAATGAACAAGATGCGTCAGGCACATGTGGCATTACAAGGTGATCTAAATGCTCTGTTACCAGCATTACAGCAGCCGCT‑AATCAAAGACTGgcatca > 2:305205/1‑143 (MQ=255) gcgcgCCCCATGCCAGTGACAGCCACATGGCTATCGCCCCGGCAGAAATGAACAAGCCGCGTCAGGCACACGCGGCAGTACAAGGTGATTTACATGCTCTGTTGCCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCaa < 1:432580/142‑1 (MQ=255) gcCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCCATGACTGGCAGCAACACt > 1:277160/1‑143 (MQ=255) acacGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACCTGTGGCATTACAAGGTGCTTTAAATGCTCTGTTCCCAGCATTACAGCCGCCGTT‑ACTCAATGCCTCGCCACAacaccccg > 1:251371/1‑139 (MQ=255) cacGCGAGTGTTATCCATATGGAGATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGGTTTAAATGCTCTGTTACCAGCAGTACAGCAGCCGTT‑AATCAATGACTGGTTGCAAGACTgcgc < 2:729634/143‑1 (MQ=255) cAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGc > 1:648842/1‑143 (MQ=255) gtgtTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTCAAATGCTCTGTTACCAGCCTTACAGCAGCC‑CTCAATCAATGACTGGCAGCAACCCGGCGCGCAGcac > 2:738053/1‑141 (MQ=255) ttATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGCGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTAGCAGCAACACTGCGCGCAGCTGCgg > 2:332381/1‑142 (MQ=255) aTCCATATGGATATCGACCCGGCAGAAATGAACCAGCTGCGTCAGGCACATGTGGCGTTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACCGCGCCCAGCCGCGtga > 2:374591/1‑143 (MQ=255) acatGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGaa < 1:10005/141‑1 (MQ=255) tgggggtagcGACCCGGGAGAAGTGAACAAGCTGCGTCAGGCACGTGTGGCATTACAAGGTGAGTTAGATGCGCTGTTACCAGCAGTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAAc < 2:211496/134‑1 (MQ=255) agACCCGGCAGAAATAAACAAACTGCGCCAGGCACACGTGGCATTACAAGGGGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑CATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGc < 2:589896/142‑1 (MQ=255) aTGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCGGTT‑AATCAATGACTGGCAGCCACACTGCGCGCAGCTGCGTGATGAACATGCCTGGGGTGACGACCAtgc > 2:39582/1‑141 (MQ=255) aTGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTCGCGACCATcc > 2:425760/1‑143 (MQ=255) tGAACAAGCTGTGTCAGCGACATTTGTCATTACATGGTGATTTGAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCTTGATGATCATTCCTGGCGTTACGACCATccc < 2:483191/143‑1 (MQ=255) gcatccggCGGCAGGCACATGTGGAATGAAGAGGTGATTGAAATGCTCTGTTACCAGCATTACAGCAGCCG‑TGAATCAATGACTGGCAGCAACACTGCGCGCAGCGGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGt < 2:729822/136‑1 (MQ=255) gcggCGTCAGGCACAGGGGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACg < 1:322474/140‑1 (MQ=255) gTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTCCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATc > 2:69354/1‑143 (MQ=255) tCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCCGCCGTT‑AATCAATGACTGGCAGCGACACTGCGCGCAGCTGCGTGATGAACATTCCTGGGGTTACGACCATCCCGGTGACGCCATCt > 2:208036/1‑143 (MQ=255) tCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGCGACGCTATCt > 1:715232/1‑143 (MQ=255) aGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAc > 2:162796/1‑143 (MQ=255) gCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAcgc > 2:553304/1‑143 (MQ=255) acaTGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTTCGTCCATCCCGGTGACGCTATCTACgcgc > 1:349448/1‑143 (MQ=255) acaTGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACgcgc > 2:163657/1‑143 (MQ=255) acaTGTGGCATTACAAGGTGATTTAAATGCTCTCTTACAAGAATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTCTCTACgcgc > 1:568217/1‑143 (MQ=255) tgGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttg > 1:705930/1‑143 (MQ=255) gCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGAACGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCAgttgtt > 1:541064/1‑143 (MQ=255) cATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAGCATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgttg < 1:291765/143‑1 (MQ=255) aTTACAAGGTGATTTAAATGCGTTGTTACCAGCTTTACAGCATCTATT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCGGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgttgt < 1:465793/143‑1 (MQ=255) ttACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgttgtt > 2:622324/1‑143 (MQ=255) ggTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaaca > 1:549385/1‑143 (MQ=255) gTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaacaa > 1:554498/1‑143 (MQ=255) aTTTAAATGCTCTGTTACCAGCATGACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCCATCTACGCGCCGTTGTTGTTAAAACAACTg > 1:524516/1‑143 (MQ=255) acaTGCCCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCgg < 1:55888/141‑1 (MQ=255) aaaTGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGGTACGACCATCCCGGTGACGCTATCTAGGCGCCGTTGTGGTTAAAACAACGGTCgg > 1:4722/1‑143 (MQ=255) aaTGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAACCAACTGTCGGa > 2:516396/1‑143 (MQ=255) tGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCGATGACTGGCCGCAACCCTGCGCGCAACTGCGTGATGGACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGCTGTTGTTAAAACAAATGGCGGagc > 2:321550/1‑141 (MQ=255) tGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAGTGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAGCATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATc < 2:588812/143‑1 (MQ=255) ccAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGAGCGTCAACGTGc > 2:424900/1‑143 (MQ=255) cagcagACGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgt < 2:470341/143‑1 (MQ=255) ggccgcCGTT‑AATCAATGACTGGCGGCAACTCTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGACTTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtc < 2:452770/139‑1 (MQ=255) agcagcCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtc < 1:162796/143‑1 (MQ=255) agcagcCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtc < 1:98709/143‑1 (MQ=255) agcCGTT‑AATCAATGACTGGCAGCAACACTGCGAGACGTGGTTTGGGGGTCGTGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGGTGTTAAAACAGCTGTCGGGTCGTAAACCGGccggtttcgacggt > 2:809525/1‑130 (MQ=255) ccGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGtga > 2:726120/1‑44 (MQ=255) ccGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGtga < 1:726120/44‑1 (MQ=255) | CGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTG > NC_000913/3951392‑3951669 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |