| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,951,535 | Δ1 bp | pseudogene (977/984 nt) | ilvG → | pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive, acetolactate synthase II, large subunit, cryptic, interrupted |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,951,533 | 0 | A | . | 97.7% | 186.4 / ‑1.6 | 45 | pseudogene (975/984 nt) | ilvG | pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive, acetolactate synthase II, large subunit, cryptic, interrupted |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (17/26); minor base G (0/1); total (18/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTG > NC_000913/3951392‑3951662 | cGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACGAGCTGCGTCCGGCACATGTGGCAGTAGAAGGTGATTTAAACGCCCTGTTACCAGCATTCAAGCAGCCGTCaa > 1:146048/1‑143 (MQ=255) ggCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTTGTTTAGGTGCTCTGTCACCAGCGTCACAGCAGCCGTTaat > 2:281322/1‑142 (MQ=255) gCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTaatc > 2:548131/1‑141 (MQ=255) cTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑CATCAATGa < 2:599195/143‑1 (MQ=255) cTGAACACCTTCGCGACACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGa < 2:500603/143‑1 (MQ=255) cacCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGgc < 2:569735/143‑1 (MQ=255) acgtgcgcgCCAGACGCCAGTGTTCTCCAGATGGAGATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGgca < 1:727383/138‑1 (MQ=255) ccTTCGCGCCACACGCCAGTATTATCCATATGGTTATCGACTCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑GATCAATAACTGgcgg > 2:598576/1‑141 (MQ=255) cTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGgcagc > 2:209941/1‑143 (MQ=255) ccgcgCCACCCGCCAGTGTGATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCaa < 2:644498/142‑1 (MQ=255) cacaCGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCAGCGTCAGGCACATGTGGCATCACAAGGGGATTTAAATGCTCTGTTACCAGCATTACACCAGCCGTT‑AAGCAATGACTGGCAGCAACACGgc > 1:516106/1‑143 (MQ=255) acGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTgcgcg > 2:687279/1‑143 (MQ=255) tgtTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGc < 2:659247/143‑1 (MQ=255) tgtTATCACTGTGGATATCGAGCCGGCAGAAATGAACAAGCGGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGc < 1:195244/143‑1 (MQ=255) tgtTAGCCATATGGAGATCGACCTGGCACAAATGAACAAGCGCCGTCAGGCACATGTGGCATTACAAGATGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGc < 1:611318/143‑1 (MQ=255) gttgactctgttGGTTACCGACCCGGCACCAATGACCAAGCTGCTTCAGGCACATGTGGCATTACAAGGTGATTTACATTCTCTGTTACTATCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGc < 1:611617/132‑1 (MQ=255) aTCCATAGGTATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGtga < 2:469344/143‑1 (MQ=255) atatGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACCGCACCCGCT‑ACTCAAGGACTGGCAGCACCACTGCCCGCAGCTGCGTGATGaa > 1:576412/1‑143 (MQ=255) atatGGATATCGACCCGGCAGAAACGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGaa < 2:663571/143‑1 (MQ=255) atGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACa < 1:687279/143‑1 (MQ=255) gATAGCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTCGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTc < 1:209344/143‑1 (MQ=255) cGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACCAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTAc < 2:537823/143‑1 (MQ=255) gaggcaaTGAACAAGCTGCGTCAGGCACATGTGGCATTACCAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGa < 1:238978/138‑1 (MQ=255) aGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCACCACTGCGCGCAGCTGCATGATGAACATTCCTGGCGTTACGAcc > 1:143319/1‑143 (MQ=255) gagcAAGCTGCGTCAGGTACATGTGGCATTAGAAGGTGATTTAAATGCTCTGGTACCAACATGATAGC‑GTTGGGCAATCAATGACTGCCACCAACACTGCGCGCAGCTGCGTCACTAACCTCGGCAGCGTAACGACCATCCCg > 1:191213/4‑143 (MQ=255) gAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCg > 2:741952/1‑143 (MQ=255) aaCAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCgg < 2:464761/143‑1 (MQ=255) aaGCTGCGGCAGGGTCCTGTGCGATCACGAGGTGATTTAAATGCTCTGGTACCAGCAGTACAGCAGCCG‑TGAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGa < 1:519294/143‑1 (MQ=255) gTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACCGCGCGCAGCGGCGTGATGACCATTCCTGGCGTTACGACCATCCCGGTGACGCTATc > 1:159428/1‑143 (MQ=255) aTTAAAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgttgt < 1:31449/143‑1 (MQ=255) ttACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgtt < 1:431244/140‑1 (MQ=255) ttACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgtt > 2:431244/1‑140 (MQ=255) agacggTGATTTAAATGCTCTGTTACCAGCATGACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaa < 2:575672/139‑1 (MQ=255) cacagttgtttATATGCGCTGGTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaaa < 1:180361/135‑1 (MQ=255) cAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaaa > 2:70105/1‑143 (MQ=255) ggTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAgaca > 2:333684/1‑143 (MQ=255) tGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaacaac > 1:59303/1‑143 (MQ=255) tttAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGAGGCTATCTACGCGCCGTTGTTGTTAAAACAACTGt < 2:562402/143‑1 (MQ=255) tgtAAATGCTCTGTGACCAGCACTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGCTGAACATTCCTCGCGTTACGAACATCCCGGTGCCGCTATCTACGCGCCGTTGTTGTTAAaacaaccaa < 2:686113/141‑4 (MQ=255) ttAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGGTAAAACAACTGTc > 2:148318/1‑143 (MQ=255) ttAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGAAGCTGCGTGATGAACATTCCTGGCGGTACGACTATCCCGGTGACGCTAGCTACGCGCCGTTGTTGGTAAAACAACTGTc > 2:147587/1‑143 (MQ=255) aTGCTCTGTTACCAGCATCACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCCGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGAt < 2:670637/143‑1 (MQ=255) gCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCg > 2:495558/1‑143 (MQ=255) tctGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTATTCTTTTGATAACGCTCGGATCGtg > 1:566430/1‑142 (MQ=255) ggTTACCAGCATTACAGCAGCCGTT‑AATCAATGCCTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTaaa < 2:435558/142‑1 (MQ=255) gTTACCAGCATTAAAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCCGCTTCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAAc < 2:431821/143‑1 (MQ=255) aCCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACGGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAGACCTg < 1:12302/143‑1 (MQ=255) ttACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGGTGTTGACACCACTCTCGGCTCGTAACCATGCggctca > 1:144466/1‑139 (MQ=255) | CGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTG > NC_000913/3951392‑3951662 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |