Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,275,067 Δ1 bp 2.7% intergenic (‑27/‑403) yjcB ← / → yjcC putative inner membrane protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,275,0670T. 2.7% 519.0 / 3.5 149intergenic (‑27/‑403)yjcB/yjcCputative inner membrane protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base T (66/78);  new base . (2/2);  total (68/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 151 total aligned reads displayed.

TGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGAC  >  NC_000913/4274793‑4275340
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                 
                  atcaCCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCgcggcg                                                                                                                                                                                                                                                                 <  2:698757/275‑1 (MQ=255)
                     aCCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGGAGCAATTAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCgttg                                                                                                                                                                                                                                                              >  1:1126121/1‑275 (MQ=255)
                       cGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGACGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCgttgtt                                                                                                                                                                                                                                                            >  1:1269144/1‑275 (MQ=255)
                          cTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGCTGTTGAGTGTGCTGGCCAGAAACAACATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGGGGTGAGGGGAGCCATCAAACAATTCCTCTGAAACGAGCCGATGCCCTTGACAGCGCGGCtttgtcaaa                                                                                                                                                                                                                                                         >  2:940852/1‑271 (MQ=255)
                             gagttttggccaatgaccGGTGATGTCCGCCATTGTCCTTCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGGTCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTGGAGTGTGCTGGCCAGAAGCATAATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCGCGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACggg                                                                                                                                                                                                                                                      <  2:44605/258‑1 (MQ=255)
                              aTGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGGGGTGAGGGTAGCCATCAAAAAATTCCTCTGAAAAGAGCCGATGCCCGGGACAGCGCGGCGGTGTTccaggg                                                                                                                                                                                                                                                      >  2:622300/1‑269 (MQ=255)
                               tgttgtGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAg                                                                                                                                                                                                                                                    <  1:647887/275‑1 (MQ=255)
                               ggttggtccAGAAAGCGGGGATGTCCGCCATGGTGATGCAAAGCAACCCCAGTGCGAACCAACAGGAGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGTACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAg                                                                                                                                                                                                                                                    <  2:239478/268‑1 (MQ=255)
                                    tgCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCATGGACAGCGCGGCGTTGTTCACGGGAGGCGTg                                                                                                                                                                                                                                               >  2:74427/1‑275 (MQ=255)
                                    tgCCAGATAGCGGTGATGTCCGCAATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAAACACGCCAGTGGTGAGGGTAGCCAACAACAAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCCCGGGGTTGTTCAGGGGAGGCggt                                                                                                                                                                                                                                               >  2:1573054/1‑273 (MQ=255)
                                      gcAGATAACGGGGATGTCCGCCATTGTGATGCCAAGCAACCCCAGTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCGGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGt                                                                                                                                                                                                                                             <  2:1330728/274‑1 (MQ=255)
                                         gATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGATCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTaca                                                                                                                                                                                                                                          >  1:854042/1‑275 (MQ=255)
                                         gATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGACAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGCCGTTGTTCCCGGGAGGCGTGGTacc                                                                                                                                                                                                                                          >  2:35968/1‑274 (MQ=255)
                                             gCGGTGATGTCCGCCATTGTTATGCCAATCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTaca                                                                                                                                                                                                                                          <  1:454828/271‑1 (MQ=255)
                                              cGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGCCATAATCAGACCGACGAAAACGGATGATGAGTGTGCTGGCCAGCAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGCAGGCGCGGCCGTGTTCACGGGAGGCGTGGtcccatctg                                                                                                                                                                                                                                     >  2:302174/1‑267 (MQ=255)
                                              cGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACAct                                                                                                                                                                                                                                        >  2:88670/1‑272 (MQ=255)
                                                        cGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCgg                                                                                                                                                                                                                           >  1:1089539/1‑275 (MQ=255)
                                                         gCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATcgc                                                                                                                                                                                                                             <  1:1546110/272‑1 (MQ=255)
                                                          ccatgtggcggCCCAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACGGCTGCTGTTCACCGCAAGCCCGACATAAGCAGACCGACGAAAACGGATTGTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCGCGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGGGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCggg                                                                                                                                                                                                                          <  2:622877/264‑1 (MQ=255)
                                                                  aTGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAg                                                                                                                                                                                                                  >  1:1347349/1‑274 (MQ=255)
                                                                     ccAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACTGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGccc                                                                                                                                                                                                                                                                               >  2:1100055/1‑210 (MQ=255)
                                                                       aaGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAaca                                                                                                                                                                                                              >  1:263350/1‑273 (MQ=255)
                                                                        aGCAACCCCATTGCGAACCAACAGGCGGTAACACCGCCCAGACCGCGGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACaa                                                                                                                                                                                                           <  2:661286/275‑1 (MQ=255)
                                                                             ccccATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATg                                                                                                                                                                                                      <  1:872741/275‑1 (MQ=255)
                                                                                   tgagAACCAACAGGCGGAAACAACCCCCAGACCCCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATGACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAaca                                                                                                                                                                                                 <  2:1056250/271‑1 (MQ=255)
                                                                                   tGCGAACCAACAGACGTAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGGGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAaca                                                                                                                                                                                                 <  1:437525/274‑1 (MQ=255)
                                                                                       aaCCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAAAATCATTACGGCACTAAGAAGTTGCAAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGA‑‑‑‑‑‑GCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCg                                                                                                                                                                                                                                                 >  1:752116/1‑216 (MQ=255)
                                                                                           aaCGGGCGGAAACAACGCCCAGACCGCTTCTGATCACCGCAAGCCCGCCATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGA‑‑‑‑‑‑GCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGt                                                                                                                                                                                                                                             <  2:752115/216‑1 (MQ=255)
                                                                                            aCAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGt                                                                                                                                                                                                                                             <  1:1371887/221‑1 (MQ=255)
                                                                                                          cGCCCAGACCGCTGCTGATCACCGCAAGCCCTACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTtgt                                                                                                                                                                         <  1:472296/275‑1 (MQ=255)
                                                                                                          cGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATACTCAGACCGACGAAAACGGATGTTGCGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAACTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTTCACTATGGCCATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTtgt                                                                                                                                                                         >  1:1463275/1‑275 (MQ=255)
                                                                                                          cGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACaaaa                                                                                                                                                                                                         >  1:550282/1‑243 (MQ=255)
                                                                                                              cAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGa                                                                                                                                                                                                     <  2:550281/243‑1 (MQ=255)
                                                                                                               aGACCGCTGCTGTTCACCGCAATCCCGACAAAATCAGACCGACGAAAACGTATGTTGAGTGGGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCAGCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAAc                                                                                                                                                                     <  2:1463274/274‑1 (MQ=255)
                                                                                                               aGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTtgtg                                                                                                                                                                        <  1:35968/271‑1 (MQ=255)
                                                                                                               aGACCGCTGCTGAGCACGGCAATCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTACTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAATCAATTCCTCTGAAAAGGGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGACGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATTAAACACTCTGTTTGTTTCATTAATTTTGTGaa                                                                                                                                                                      <  2:337106/273‑1 (MQ=255)
                                                                                                               aGACCGCTGCGGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTtgtg                                                                                                                                                                        <  2:1347348/271‑1 (MQ=255)
                                                                                                                    gctgctGATCACCGCAAGCCCGACATAATCAGACCTACGACAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCAATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATc                                                                                                                                                               <  1:1579502/275‑1 (MQ=255)
                                                                                                                    gctgctGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGTAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATc                                                                                                                                                               <  1:622301/275‑1 (MQ=255)
                                                                                                                    caggctgATCACCGCAAGCCCGACAAAATCAGAGCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATc                                                                                                                                                               <  1:1537748/272‑1 (MQ=255)
                                                                                                                     ctgctgATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATca                                                                                                                                                              >  1:1186479/1‑275 (MQ=255)
                                                                                                                         tgATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAaca                                                                                                                                                                                                 <  1:348686/236‑1 (MQ=255)
                                                                                                                                cGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCa                                                                                                                                                                                                                                                          >  1:36395/1‑172 (MQ=255)
                                                                                                                                cGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACCCTGTTTGTTTCATTAATTTTGTGAACTATATCACCACTGAttgt                                                                                                                                                   >  1:713815/1‑275 (MQ=255)
                                                                                                                                  cAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGACAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGCTGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAAAACACCGTTTGTTTCATTAATTTTGGGAACTATATCACAATTAAttgtt                                                                                                                                                  >  2:1537747/1‑274 (MQ=255)
                                                                                                                                    aGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACggg                                                                                                                                                                                                                                                      <  2:36395/172‑1 (MQ=255)
                                                                                                                                     gCCCGAAATAAACATACCGACCAAAACGGATGTTGGGTGTGCTGGCCAAAAACATCATTATGGCACTAAGAAGTTGCCAGCTAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATtgtttgtt                                                                                                                                              <  1:1573055/275‑1 (MQ=255)
                                                                                                                                       cctgcaaaaTCAGACCGACGAAAACGGATGTTTATGGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCAAGTGGTGATTGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCTGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAg                                                                                                                                            <  2:110958/268‑1 (MQ=255)
                                                                                                                                       ccaaCAAAATCAGACCTACTAAAACGGATGTGGTGTGTGCTGGCCAGAAACAGCATTACGGCCCTAAGAAGTTGCCAGCGAAGAAGACCCACGCCAGTGGTTAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGCTGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGTCTAGCGCGGGGCGTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTTTTAg                                                                                                                                            <  2:433130/272‑1 (MQ=255)
                                                                                                                                       ccGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGATCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAg                                                                                                                                            <  2:854041/275‑1 (MQ=255)
                                                                                                                                        cGAAATAAGCAGACCGACGAAAAGGGATGTTGAGTGTGCTGGCCAGAAAAATCATTACGGCACTGAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAg                                                                                                                                            <  2:523481/274‑1 (MQ=255)
                                                                                                                                          aCATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAAt                                                                                                                                                                                                       >  1:632322/1‑213 (MQ=255)
                                                                                                                                          aCATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGAttgtttg                                                                                                                                                >  1:523482/1‑268 (MQ=255)
                                                                                                                                             tAATCAGACCGACGAAAACGGATGTTGAGGGTGATGGCCAAAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAAAAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCatat                                                                                                                                      <  2:592150/275‑1 (MQ=255)
                                                                                                                                                   gACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACtat                                                                                                                                                                  >  1:1555415/1‑241 (MQ=255)
                                                                                                                                                    aCCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCACTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTaa                                                                                                                                                                               >  2:839836/1‑227 (MQ=255)
                                                                                                                                                      cgacgaAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTg                                                                                                                             <  1:940853/275‑1 (MQ=255)
                                                                                                                                                      cgacaaAAACGGATTTTGAGTGTGCTGGCCGGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGGGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTg                                                                                                                             <  2:1389675/275‑1 (MQ=255)
                                                                                                                                                       gacgaAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATca                                                                                                                                                              <  2:1555414/241‑1 (MQ=255)
                                                                                                                                                        acgaAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGaa                                                                                                                                                                      <  1:1387967/232‑1 (MQ=255)
                                                                                                                                                          gaAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCAtt                                                                                                                                                                                 >  1:527247/1‑219 (MQ=255)
                                                                                                                                                           nanaCTGATGTTGAGGGTGCCGGCCAAAAACATCAGTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAAATCCTCTGAAAATAGCCGATGCCCTGGACAGCGCCGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGTGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACttt                                                                                                                        <  2:1158827/274‑1 (MQ=255)
                                                                                                                                                            aaaCGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTg                                                                                                                                                                                                                     <  2:625380/181‑1 (MQ=255)
                                                                                                                                                            aaaCGGATGTTGAATGTGCTGGCCAGAAGCATCATTACGGCAATAAGAAGTTGCCAGCGAAGAAGAACCCCGCCATTGGTGAGTGTGGCCATCAAACAATTCCTCTGAAAATAGCCGATGCCCTGGGCAGCGCAGCGTTGTTCACTGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTtgtg                                                                                                                                                                        <  1:429379/226‑1 (MQ=255)
                                                                                                                                                              aCGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAAtt                                                                                                                                                                             <  2:527246/219‑1 (MQ=255)
                                                                                                                                                                   tGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACACCATTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTg                                                                                                                   <  1:1395895/272‑1 (MQ=255)
                                                                                                                                                                      tGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACaaaa                                                                                                                                                                                                         <  1:231343/183‑1 (MQ=255)
                                                                                                                                                                                  ccAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGaa                                                                                                                                                                                                    >  2:1406826/1‑176 (MQ=255)
                                                                                                                                                                                      aaaCATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAacac                                                                                                                                                                                                <  1:1406827/176‑1 (MQ=255)
                                                                                                                                                                                      aaaCATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAAAACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTTTACAGATTATGTGGttttt                                                                                             >  2:1314117/1‑275 (MQ=255)
                                                                                                                                                                                       aaCATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGttttt                                                                                             <  1:1244946/274‑1 (MQ=255)
                                                                                                                                                                                       aaCATCAATACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCCCGCCAGTGGTGAGGGTAGACATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGTGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGttttt                                                                                             <  1:1481256/274‑1 (MQ=255)
                                                                                                                                                                                           tcatTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCCGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTCTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTTTACCGATTTTGTGGTTTTTTAGTg                                                                                        >  2:466931/1‑275 (MQ=255)
                                                                                                                                                                                           tcatTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTg                                                                                        >  1:1009949/1‑275 (MQ=255)
                                                                                                                                                                                                      cTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAggg                                                                              >  2:206914/1‑274 (MQ=255)
                                                                                                                                                                                                         agaagTTGCCAGCGAAGAAGAACAACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAAAACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTTCAGATTATGTTGTTTTTCAGTGGCATTAAGGGCat                                                                           >  1:1338152/1‑274 (MQ=255)
                                                                                                                                                                                                              ttGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGAc                                                                                                                                                                                                                                                                          >  2:677886/1‑78 (MQ=255)
                                                                                                                                                                                                                gCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTtg                                                                                                                                                                          >  2:556990/1‑172 (MQ=255)
                                                                                                                                                                                                                 ccAGCGAAGAAGAAACCCGCCAAGGGGGAGGGTAGCCATCAAACAATTCCTCGGAAAAGGTCCAGCCCTGGACAGCGCGGCGTTGTTCACTGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAACATGAAACGCTCTGTTTGTTTCATTAATGTTGTGAACTATATCACAATTGATTGTTTGTTAGACATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATGTCTTCccgc                                                                 <  2:25822/274‑1 (MQ=255)
                                                                                                                                                                                                                  cAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAgcg                                                                                                                                                                                                                                                                      <  1:677887/78‑1 (MQ=255)
                                                                                                                                                                                                                  cAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGTAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTtgtg                                                                                                                                                                        <  1:979350/172‑1 (MQ=255)
                                                                                                                                                                                                                  cAGCGAAGAAGAACCACACCAGGGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTGGCCAGATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCccgc                                                                 <  1:899343/275‑1 (MQ=255)
                                                                                                                                                                                                                    gCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGaa                                                                                                                                                                      <  1:556991/172‑1 (MQ=255)
                                                                                                                                                                                                                     ggataagagacagcGCAAGTGGTGAGGTTAGCCATCAAACAATTCCTCTGAAAAGAGCCGAGGCCCTTGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCcgccgc                                                              <  1:829343/262‑1 (MQ=255)
                                                                                                                                                                                                                          aagaaCCACGCCAGGGGGGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGAGGCGTTGGTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTGGTTAGCCATATTAGGCCGTGAATTTTATTGCTGTACAGATTATGTGGTTTTTCAGGGGCATTAAGGGCATATCGTCCCGCCGCCTCTg                                                         <  2:1269143/275‑1 (MQ=255)
                                                                                                                                                                                                                            gaaCAACGCCAGGTGTGAGGGTAGCCATCAAACAATCCCGCGTAAATGGGCCCATGCCCTGGACAGGGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCa                                                       <  2:713814/275‑1 (MQ=255)
                                                                                                                                                                                                                             aaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCa                                                       <  1:1314118/274‑1 (MQ=255)
                                                                                                                                                                                                                               ccACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCa                                                                                                                                                                                                                                                          >  1:88543/1‑77 (MQ=255)
                                                                                                                                                                                                                                   gCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACggg                                                                                                                                                                                                                                                      <  2:88542/77‑1 (MQ=255)
                                                                                                                                                                                                                                             aGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGTGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAaattaa                                      >  1:1505512/1‑275 (MQ=255)
                                                                                                                                                                                                                                                 tAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTTTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACAATCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTCGGCCGTGACTTTTATTGTTGTACAGCTTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTTCCGCCGGCTCTGCATTCCTGTAGGAAATTATtttt                                  >  1:4571/1‑275 (MQ=255)
                                                                                                                                                                                                                                                  aGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGAttgtttg                                                                                                                                                >  1:574864/1‑164 (MQ=255)
                                                                                                                                                                                                                                                      aNCAAACAATTCNTCTNANNAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTg                                                                                                                             <  2:1186478/179‑1 (MQ=255)
                                                                                                                                                                                                                                                         aaaCAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCa                          >  1:11378/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                         aaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAgg                                             >  2:511814/1‑240 (MQ=255)
                                                                                                                                                                                                                                                                             gCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAaat                                         <  1:511815/240‑1 (MQ=255)
                                                                                                                                                                                                                                                                              ccGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCtgt                                                                                                                                                                                           >  1:20771/1‑93 (MQ=255)
                                                                                                                                                                                                                                                                                 aTGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGa   >  1:764827/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                                 aTGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGAc  >  1:820775/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                                  tGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCtgtttgt                                                                                                                                                                                       <  2:20771/93‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                 
TGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGAC  >  NC_000913/4274793‑4275340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: