New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 3714532195 (1.300)3 (0.020) 3/454 NT 1.5% coding (1862/2334 nt) bisC biotin sulfoxide reductase
?NC_000913 3714545 = 196 (1.320)coding (1849/2334 nt) bisC biotin sulfoxide reductase
Rejected: Frequency below/above cutoff threshold.

TCCCTCGCTAATGACCGCTCCGGCAAGAATTTGCCCGCGTGCGTTCCACAACCGAACAGTATCGCCATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGCTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3714282‑3714532
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAA  >  NC_000913/3714545‑3714818
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
TCCCTCGTGAATGACCGCTCCGGCAAGAATTTGCCAGCGTGCGTTCCACAACCGAACAGTATCGCATTCTTGTATGCCGCGCTCCTGGGCGTCATCAGGATGAATGGTGACAGGCTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCGGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGCCCAGGGCAATCCGGGTAACCGTAAT                                                                                                                                                                                                                                                            <  1:601411/275‑1
                                                                             CGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGCTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAG                                                                                                                                                                                                                                                                                      >  1:593943/1‑172
                                                                                 CTCCTGGGCATCGTCAGGATGAATGGTGACAGGCTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAG                                                                                                                                                                                                                                                                                      >  1:163947/1‑168
                                                                                                                                                                  CTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGG                                                                                                                      >  2:537049/1‑247
                                                                                                                                                                      CTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGG                                                                                                                  <  1:537050/247‑1
                                                                                                                                                                                                                                       cccctggcacattggggtCCCAGGGCAATCCGGGGAACCGTAATCGGTAATCCGCGGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTACCGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAAGAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTACCTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATAGAACGTTTCCAGCCATTGCAGCT                     <  2:1247761/257‑1
                                                                                                                                                                                                                                       ccacgtgcacataggcgGCCCCGGGAAATCCGTGTAACAGTAATCGGCAATCCGCTGTGAGAATATTGCAAGCTTGCCGCGGGCGGTTTTTAACGGATGCGCCAGCGGGTGGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGTTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATAACACGTTTCCAGCCATTGCAGCT                     <  2:798328/258‑1
                                                                                                                                                                                                                                                          CCAGGGCAATCCGGTTAACCGTAATGGGCAATACGCAGTGAGAAGATTTCAATCTTGCCGGTGGCGGTTTTTAACGGAGGCGCCAGCGGATCGCTGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATAAAACGTTCCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAA  <  2:1079574/275‑1
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
TCCCTCGCTAATGACCGCTCCGGCAAGAATTTGCCCGCGTGCGTTCCACAACCGAACAGTATCGCCATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGCTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3714282‑3714532
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAA  >  NC_000913/3714545‑3714818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.