Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	523,618	A→G	13.5%	S120G (AGC→GGC)	rhsD →	Rhs protein with DUF4329 family putative toxin domain, putative neighboring cell growth inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	523,618	0	A	G	13.5%	206.9 / 2.8	90	S120G (AGC→GGC)	rhsD	Rhs protein with DUF4329 family putative toxin domain, putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (22/52);  new base G (6/9);  total (29/61)
Fisher's exact test for biased strand distribution p-value = 5.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑ACTGGGGTGGTCTGAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTGCCGCCGTACCGGGTGCT  >  NC_000913/523345‑523886                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                  gnggggccacccGGCGGGGGGGGCGCGGTGGGTGCGGGCGGGATGACTTCGGGGAACCCGGTAAATCCGCTGGTAGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGGTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGa                                                                                                                                                                                                                                                                                   <  2:1146548/264‑1 (MQ=25)   cgcgcCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑Gc                                                                                                                                                                                                                                                                                >  1:464748/1‑274 (MQ=255)   cgcgcCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCa                                                                                                                                                                                                                                                                               >  1:1477338/1‑275 (MQ=255)                gggtggtgCGCGGGGTGTCCGGTGGGGATGACTTCGGGCGAACCGGGAAGTCCGCTGCTGGGGTCGAAGTTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCTGCCGGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGc                                                                                                                                                                                                                                                                  <  2:1036304/269‑1 (MQ=37)                  ggcgtgCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATCTCCGCTTACAGCTACGTGATGCCGGCCTGATACTCAACCACAACGGCGGGCGGG‑GCAATC‑CCTTTGAGcccg                                                                                                                                                                                                                                                               >  2:447627/1‑273 (MQ=255)                      tgCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGACGGCGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCAGCTGCCGTTCCTTTTCTCCCGCACCTACAGCAGCTACCGGACGACGACGCCTGCCCCGGTGGGCGGTTTCGGCCCCGGCTGGAAAACGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACCCAACGACAACGGCGGGCGGG‑GCATTC‑TCTTTGCGCCcccgct                                                                                                                                                                                                                                                           >  1:138589/1‑275 (MQ=255)                      tgCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTCAGCCgctgcc                                                                                                                                                                                                                                                           >  1:1101226/1‑274 (MQ=255)                        cTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGCCGGGCGGA‑GACTTC‑ACTTTGAGCCgctgct                                                                                                                                                                                                                                                           >  1:778388/1‑273 (MQ=255)                             tgtgtCCGGGCGGGATGACTTCGGGCACCCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTCCAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATCCTCAACGACAACGGCGGGGGGAGGCATCC‑CCTTT‑CGCCCCTGCT‑CCCggg                                                                                                                                                                                                                                                    >  2:214260/1‑274 (MQ=25)                                 tCCGGGCGGCATGACGGCGGGCAACCCGGGAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCAAAGCGGACCTT‑GCGCTGCCCGTCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCggggg                                                                                                                                                                                                                                                  <  1:1345478/272‑1 (MQ=255)                                  ccGGGCGGGGTGACTTCGGCCAACCGGGTAAATCCCCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAgg                                                                                                                                                                                                                                               <  2:464747/274‑1 (MQ=255)                                       cgggATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGGT‑GCCGGGGGAGGCGgtg                                                                                                                                                                                                                                          <  1:389271/274‑1 (MQ=255)                                        gggATGCCTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGTTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTa                                                                                                                                                                                                                                        <  1:1391068/275‑1 (MQ=255)                                         ggATGGCGTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGCCGGACCTT‑GCCCGGCCCGGCCCCCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGGCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCGGACATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTa                                                                                                                                                                                                                                        <  2:474534/274‑1 (MQ=255)                                           aTGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GTCGGGGGGGGCGGTGTaacg                                                                                                                                                                                                                                     >  1:38564/1‑272 (MQ=255)                                             gcctgcGGGCAACCCGGTACATCCGCTGCTGGGGGCGAAGGTGCAGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTCCAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGATGTACAGc                                                                                                                                                                                                                                    <  1:847060/269‑1 (MQ=255)                                                ttCGGGCAACCCGGTAGATCCCCGGCTGGGGGCGAAGGTGCGGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCa                                                                                                                                                                                                                                <  2:38564/275‑1 (MQ=255)                                                ttCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCCACGTGATGACGGACTGATACTCAACAACAACGGCGGGCGGG‑GCCATA‑CCTTTGAGCCCCCGCC‑TCCGGGGGGGGCGGTGGACAGCCGcc                                                                                                                                                                                                                                >  2:35725/1‑274 (MQ=37)                                                gtCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCa                                                                                                                                                                                                                                <  1:664854/274‑1 (MQ=255)                                                 gcGGGCAACCCGGTAAATCCGCTGCTGGGGACGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCa                                                                                                                                                                                                                                <  1:992927/273‑1 (MQ=255)                                                        aCCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAACCGCCTTCTGATATCCGCTTCCAGCTACGTGATGACGGACGGATACTCAACGACAACGGCGGGCGGA‑GACTTCTACTTT‑AGCCGCTGTC‑TCCGgggggggggggggaacgcagcagggaggga                                                                                                                                                                                                                        >  2:1341368/1‑255 (MQ=25)                                                         cccGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGG‑GCATTC‑ACTTTGAGCCCCTGCTGGCCGGGGGGGGGGGGGTGCacagccgggggtcaa                                                                                                                                                                                                                        >  2:1319132/1‑261 (MQ=25)                                                              tAAAGCCGCTGCTGGGGCCGAAGGGGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTGCATTCTCTCCCGCACCGACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACGGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGTGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTgg                                                                                                                                                                                                                  <  1:657974/275‑1 (MQ=255)                                                               aaaTCCGCTGCTGGGGGAGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTACACTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGG‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGc                                                                                                                                                                                                                 <  1:32111/275‑1 (MQ=255)                                                                      ctgctgGGGGCGAAGGTGCTGCCCGGCGAGGCGGACCTT‑GCGCTGCCCGGCCCGCGGCCGTTCATTCTCTCCCGCACTTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTTGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTgc‑g                                                                                                                                                                                                         <  1:1341369/275‑1 (MQ=255)                                                                       tgctgGGGGCGAAGGTGCTGCCCGGCGAGCCGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTgc‑gc                                                                                                                                                                                                        <  1:704732/275‑1 (MQ=255)                                                                          gggtgcggagggggccgcCCGGCGGGCCGGCCCTT‑GCTCTGCCCGGCCCGCTGCCGTTCGTTCGCTCCCAAACCTACTGCAGCTACCGGACGAAGAAGGCTGATCGGGTGGGCGTTTTCGCCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACACCGGCGGGCGGA‑GCATTC‑ACTTTGGGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTgc‑gc                                                                                                                                                                                                        <  2:1284572/256‑1 (MQ=255)                                                                                       gctgcCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGgcagca                                                                                                                                                                                         <  1:107149/274‑1 (MQ=255)                                                                                             cGGCGAGGCGGACCTT‑TCGCTGCCCGGCCCGCTGCCGTTCCTTCTCTCCCTCACCTACAGCAGCTACCGGACGAAGACGCCTGGACCGGTGGGCGTTTTCGGCCCCGGCTGGAAGGCGCCTTCTGATATCCGCTTACAGCTACGTTATGACGGACTGATACTCAACGACAACGGCGGTCGGG‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCg                                                                                                                                                                                   <  2:665524/274‑1 (MQ=255)                                                                                             cGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCTTTC‑ACTTTGAGCCGCTGCT‑GCCCGGGGGGGGGGGGTCCGGCCGCAGTGGCTCAATGTGGGTGGTTC‑GCCGTGGTGAGGACGCACAACCgg                                                                                                                                                                                  >  2:135319/1‑275 (MQ=37)                                                                                             cGGCGAGACGGACCTT‑GCGCTGCCCGACCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCg                                                                                                                                                                                   <  1:1244610/274‑1 (MQ=255)                                                                                               gCGAGACGGCCCTTGGCGGTGCCTGG‑CCGCCGCCGTTCATTGTCTCCCGCGCCTACGGCAGGTACGGGACGAAGACGCCTGAGCCGGTGGGCGTTTTCGGCCCCGGCTGGATAGCACCTTCTGAGATCCGCGTACGGCTACGGGATGACTGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGGGCCGCTGCG‑GCCGGGGTAGGCGGTGTACAGCCGCAGGGAGTCAATGTGGCTGGTGC‑GCGGAGGTAAGGCAGCACAGCCGGa                                                                                                                                                                                 <  2:138588/274‑1 (MQ=255)                                                                                                            t‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTgg                                                                                                                                                                   <  1:135320/275‑1 (MQ=255)                                                                                                                gcTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGgcg                                                                                                                                                                 <  1:35725/274‑1 (MQ=255)                                                                                                                 cTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGa                                                                                                                                                                                 <  1:748597/257‑1 (MQ=255)                                                                                                                  tccccggaccgCTGCCGTTCAGTCTCTCCCGCACCTACGGTAGCTACCGGACGAAGACGCCTGGACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGcgcg                                                                                                                                                               <  1:886207/272‑1 (MQ=255)                                                                                                                    cccggcccgCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCCTTC‑CCTCTTAGCCGCCCCT‑GCCCGGGGGGGCGGGGTGCAGCCGCAGCGAGTCAAAGTGGCGGCGGC‑GCGGGGGGAAGGGGCAACACCCGGACGGCCATCCgacgggggggc                                                                                                                                                             >  2:1051700/1‑264 (MQ=21)                                                                                                                    cccggcccgCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCgg                                                                                                                                                              <  1:1319133/273‑1 (MQ=255)                                                                                                                         cccgCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCTTTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTgc‑gc                                                                                                                                                                                                        <  1:1103478/226‑1 (MQ=255)                                                                                                                              tGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGG‑GCACTC‑ACTTTGTGCCCCCGCC‑GCCCGGGGAGGGGGGGTTACGCCCCCGTGGGGCAAAGTGGGCCGTGC‑GCGGGGGGAAGGCGGCACCGCGCGGCCGCCATCAACCCGCGCGGACGTggggggg                                                                                                                                                   >  2:484449/1‑273 (MQ=21)                                                                                                                              tGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCa                                                                                                                                                                                                                                >  1:497505/1‑198 (MQ=255)                                                                                                                                  gTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGa                                                                                                                                                                                                                            <  2:497504/198‑1 (MQ=255)                                                                                                                                        tctctcCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGCGGGGGGCGCTGCCGCCgg                                                                                                                                        <  1:447628/275‑1 (MQ=255)                                                                                                                                            tcCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCGACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGatat                                                                                                                                    <  1:1051701/275‑1 (MQ=255)                                                                                                                                            tcCCGCACCGACGGCAACTACCGTGCGACAAAGCGTGAACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCAGTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGatat                                                                                                                                    <  2:865993/275‑1 (MQ=255)                                                                                                                                               cGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCggggg                                                                                                                                                                                                                                                  <  1:1018516/163‑1 (MQ=255)                                                                                                                                                 cACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑AATTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGGGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGGCTAGCCGGACGGGCACACGCTGGCCGGGCTGTGGGGGGCGCTGCCGCCGGATAAACGGt                                                                                                                               >  1:1585351/1‑275 (MQ=255)                                                                                                                                                  aCCGACGGCAGCTGCCGGACGAAGACGCCTGCACCGGTGGGCGTTGTCGGCCCCGGCGCGAAGGCGCCTTCTGATATCCGCTTACAGCTAGGTGATGACGGACTGACACTCAACGACAACGGCGGGCAGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCGGTGAGTGAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGCGCGCTGCCGCCGGATATCCGGtt                                                                                                                              <  2:87803/275‑1 (MQ=255)                                                                                                                                                      aCAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGTA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTgc‑gc                                                                                                                                                                                                        <  1:535229/197‑1 (MQ=255)                                                                                                                                                         gcagcTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCg                                                                                                                       <  1:484450/275‑1 (MQ=255)                                                                                                                                                         gcagcTACCGGACGAAAACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCg                                                                                                                       <  1:48149/275‑1 (MQ=255)                                                                                                                                                            gcTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGG‑GCATTC‑ACTTTGAGCCGCTGCT‑ACCGGGGGAGGCGGGGTGCAGCCGCCGCGAGGCAATGTGGCTGGCGC‑GCGGGGGTAAGGCAGCACAGCCGGACGGCCCTACGCTGGCGCGCGGGGGGGGGGCGCTGGCCCCCGGCACCCGGTTCAGCCCGcg                                                                                                                     >  2:788095/1‑273 (MQ=255)                                                                                                                                                             cTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCTCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGGGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATAAGCTGGCGCGGGTGTGGGGGGCGCTGCCGCCGGATATACGGGTAAGGCCGcca                                                                                                                    >  1:1209843/1‑272 (MQ=255)                                                                                                                                                              tACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATTCGCCGGCGCGGCCGTGGGGGGCGCGGCCGCCGGATATTCGGTTAAGCCCCCaccc                                                                                                                  >  1:76051/1‑272 (MQ=255)                                                                                                                                                              tACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCCCTGCT‑GCCGGGGGGGGCGGGGTTCAGCCGCAGTGAGTCAATTGGGGCGGTGCGGGGGGGGTAGGGCGCCCCGGCCGGCGGGCCAAACCCGGGCCGGGCGGGGGGGGGGGTGGCCG‑CGGGGATCCGGttt                                                                                                                             >  2:1320397/1‑263 (MQ=255)                                                                                                                                                                  ggACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGcc                                                                                                                         <  1:1320398/264‑1 (MQ=255)                                                                                                                                                                   ggagAAGACGCCTGCACCGGTGGGCTTTTTCGGCCCCGGCGGGAAAGCGCCTTCGGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGGCACCGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTAcc                                                                                                             <  2:25832/272‑1 (MQ=255)                                                                                                                                                                     cGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTg                                                                                                           <  2:372559/275‑1 (MQ=255)                                                                                                                                                                            gCCTGAACCGGGGGGCGGTTGCGGCCCCGGCTGGGAAGGGCCTTCGGATATCCGCTTACAGGTACGTGGTGACGGACTGATGCTCAGCGACAACGGCGGGCGGA‑GCCTTC‑ACTTTGGGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGGGAGGCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGAc                                                                                                      <  2:547244/273‑1 (MQ=255)                                                                                                                                                                              cTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGC‑GCATTC‑ACTTTGACCCGCTGCT‑GCCGGGGGAGGCGGGGTACAGCCCACGTGAGTCAATGTGGCTGGGGC‑GCGGGGGTAAGGAAGCCCAGACGGGCAGCCATACGCCGGCGCGGGCGGGGGGGGCGCGGCCGCCCGGTTCCCGGTTTAGGCCGCACATTTTCCTGGCGACCaca                                                                                                  >  2:1436583/1‑273 (MQ=255)                                                                                                                                                                              cTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATCC‑ACTTTGAGCCGCTGCT‑GCCg                                                                                                                                                                                                                                                      >  1:1552256/1‑128 (MQ=255)                                                                                                                                                                              cTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCCTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑GCTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGGCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGGGGGGGGCGCGGCCGCCGGCTGTCCGGTTAAGCCCGCCTGTTTACCTGGCGACCCAc                                                                                                  >  1:215498/1‑275 (MQ=255)                                                                                                                                                                                  aCCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATCC‑ACTTTGAGCCGCTGCT‑GCCggggg                                                                                                                                                                                                                                                  <  2:1552255/128‑1 (MQ=255)                                                                                                                                                                                    cGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGGGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCCGTGGGGGGCGCTGCCGCCGGGTATCCGGTTAAGCCCGCATCTGTACCTGGGGCCCAACCGCGca                                                                                            >  1:1180414/1‑275 (MQ=255)                                                                                                                                                                                     ggTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGG‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTgc‑gc                                                                                                                                                                                                        <  1:349960/166‑1 (MQ=255)                                                                                                                                                                                          gCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAgggg                                                                                      <  1:398590/275‑1 (MQ=255)                                                                                                                                                                                            ggttgcGGCCCCGGCTGGAAAGCGCCGTCTGATATCCGCGTACAGCTACGTGATGACGGACTGTTTCTCAACGACAACGGCGGGCGGG‑GCATTC‑ACTTTGAGCCGCTGGT‑GCCGGGGGAGGCGGTGGACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGcc                                                                                    <  2:243633/270‑1 (MQ=255)                                                                                                                                                                                            gTTGTCGGCCCCGGCCGGAAAGGGCCTGCTAATTGCCGCCTACAGCCACGGGATTACGGACTGCAACTCACCGAGAACGGCGGTGTGG‑GCCTTC‑ACTTTGGGCCGCTGGT‑GCCGGGGGAGGCGGTGTACAGCCGCCGGGAGGCAATGTGGCTGGTGC‑GCGGTGGTAAGGCGGCACAGCCGGAAGGCGATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCTGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGcc                                                                                    <  2:1101225/275‑1 (MQ=255)                                                                                                                                                                                              tttCGGCCCCGGCTTGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGGCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTaa                                                                                                                            >  1:756846/1‑233 (MQ=255)                                                                                                                                                                                                 cGGGCCCGCCTGGAAGGCGCCTTGTGGTAGCCGCTTACAGCTACGTGAGGACGGACGGGTAGCAAATGACAACGGCGGGGGGA‑GCATTC‑GCTGTGAGCGGCTGGT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCGGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCgtggt                                                                               <  2:13718/275‑1 (MQ=255)                                                                                                                                                                                                  ggCCCCGGCTTGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGccc                                                                                                                        <  2:756845/233‑1 (MQ=255)                                                                                                                                                                                                      ccGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑a                                                                         <  1:1321988/275‑1 (MQ=255)                                                                                                                                                                                                      ccGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑a                                                                         <  1:214261/275‑1 (MQ=255)                                                                                                                                                                                                      ccGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑a                                                                         <  1:322757/275‑1 (MQ=255)                                                                                                                                                                                                        ggCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAAtg                                                                                                                                                                                                                     >  1:91688/1‑135 (MQ=255)                                                                                                                                                                                                            ggAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGc                                                                                                                                                                                                                 <  2:91687/135‑1 (MQ=255)                                                                                                                                                                                                                   gcCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGGGAGTCAATGTGGCTGGTGG‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCGGTGGGGGGCGCTGCCGCCAGATATCCGGTTAAGCCGGCATCTTTACCTGGCGCCCAACAGCGCACAGGGGCCGTGGGGGATCACGGGGGGGGTcg                                                             >  1:1582632/1‑274 (MQ=255)                                                                                                                                                                                                                               ccGCGTACAGCTACGGGATGCGTGGCTGATCCTCAACGACAACGGCGGGCGGG‑GCATTC‑ACTTTGGGCGGCTGCT‑GCCGGGGGGTGCGGTGTACAGGCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑ACTGGGGTGGTCTGAGcgggtgcc                                                  <  2:778387/273‑1 (MQ=255)                                                                                                                                                                                                                                    tACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGcaca                                                                                          <  1:1306873/229‑1 (MQ=255)                                                                                                                                                                                                                                      cAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCgtggt                                                                               <  1:1124806/238‑1 (MQ=255)                                                                                                                                                                                                                                                 tgaCGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAg                                                                                                                           >  1:1002919/1‑183 (MQ=255)                                                                                                                                                                                                                                                     ggACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCg                                                                                                                       <  2:1002918/183‑1 (MQ=255)                                                                                                                                                                                                                                                               agagggggataaggcaagggA‑GCGTTC‑GCTTTGGGCGGCTGCT‑GCGGGGGGGGGCGGTGTACGGCCGCAGTGAGTTAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCCGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACAAACAGCGCACAGGGGCCGTGGTGGAT‑ACTGGGGTGGTCTGAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTgccg                <  2:432371/257‑1 (MQ=255)                                                                                                                                                                                                                                                                  ccgcaaaCGGCGGGCGGG‑GCATTC‑ACTTGGTGCCGCTGCT‑GCCGGGGGAGGCGGGGGACAGCCGCAGTGGGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCCCAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑ACTGGGGTGGTCTGAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTgccgcc              <  2:76050/269‑1 (MQ=255)                                                                                                                                                                                                                                                                   cGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGGGTAAAGCCGCCGGGGGTCAATGTGGCCGGTGC‑GCCGTGGTAAGGCAGCGCAGCCGGACGGCCAAACACCGGCGCCGCTGGGGGGGGCGCCGCCGCCGGCGATCCGGTGAAACCCGCATCTTTACCTGGCGCCCAACAGCGCACCGGCGGCGCGGTGGGG‑ACTGGGGGGGTCTGGGGGGGTGCGGGGGGCGGAGCGCGGACTCACACcgccgcgggccccgg            >  2:1407247/1‑266 (MQ=255)                                                                                                                                                                                                                                                                      cgacgtagggcggA‑GCTTTC‑ACATTGGGCCGCTGCC‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGGGTCAATGTAGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTAGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑ACTGGGGTGGTCTGAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTGCCGCCGTAc          <  1:198431/267‑1 (MQ=255)                                                                                                                                                                                                                                                                      cAACGGCGGGCGGA‑GCATTC‑ACGTTGGGGCGCTGCT‑GCCGGGGGGGGCGGTGTACGGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑ACTGGGGTGGTCTGAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTGCCGCCGTAcc         <  1:538268/275‑1 (MQ=255)                                                                                                                                                                                                                                                                       aaCGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGACGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑ACTgggg                                                                   <  1:1011938/216‑1 (MQ=255)                                                                                                                                                                                                                                                                        aCGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑ACTGGGGTGGTCTGAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTGCCGCCGTACCgg       <  1:915608/275‑1 (MQ=255)                                                                                                                                                                                                                                                                          ggcgggcgGG‑GCATTC‑ACTTTGATCCGCTGCT‑GCCGGGTGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑ACTGGGGTGGTCTGAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTGCTGCCGTACCggg      <  1:15326/274‑1 (MQ=255)                                                                                                                                                                                                                                                                              ggcggG‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACGGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑ACTGGGGTGGTCTGAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTGCCGCCATCCCggtaagc  <  1:773254/274‑6 (MQ=255)                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                  GGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT‑GCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGA‑GCATTC‑ACTTTGAGCCGCTGCT‑GCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGC‑GCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCGACCAACAGCGCACAGGGGCCGTGGTGGAT‑ACTGGGGTGGTCTGAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTGCCGCCGTACCGGGTGCT  >  NC_000913/523345‑523886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑