Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,400,524 G→A 7.9% R188C (CGC→TGC)  mreB ← cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,400,5240GA 7.9% 296.9 / 12.0 97R188C (CGC→TGC) mreBcell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand):  ref base G (43/46);  new base A (5/3);  total (48/49)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

AGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTG  >  NC_000913/3400237‑3400809
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                             
aGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATgcg                                                                                                                                                                                                                                                                                               <  1:232690/288‑1 (MQ=255)
aGCCAATTCCGGCGGGCACGGTTCCAGTGCAACCATTACCGCGCTAACAATACCGGTCAGCGGTTCCTGCAGTGCTTCAAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCAGCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATgcg                                                                                                                                                                                                                                                                                               <  2:559786/288‑1 (MQ=255)
      ttCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACagaa                                                                                                                                                                                                                                                                                        >  1:738053/1‑289 (MQ=255)
       tacggcggGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCGTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACagaa                                                                                                                                                                                                                                                                                        <  1:750447/286‑1 (MQ=255)
       tCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACagaag                                                                                                                                                                                                                                                                                       <  1:659053/289‑1 (MQ=255)
       tCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACagaa                                                                                                                                                                                                                                                                                        <  2:738053/288‑1 (MQ=255)
       tCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATGTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACagaag                                                                                                                                                                                                                                                                                       <  1:403405/289‑1 (MQ=255)
         cggcggGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAagag                                                                                                                                                                                                                                                                                     <  1:742809/289‑1 (MQ=255)
                cACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTaa                                                                                                                                                                                                                                                                               >  1:570080/1‑288 (MQ=255)
                cACTGTTACAGTGCAACCATTATCGCGCTCACAATACCGGTAAGCGGTTCCCGCTGTTCTTCGCGGATTTCATTGGAGTTCAGGGTACAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGTACTTCGTTTTCACGGACTTCATCCCCCGGATAAGCCGAACCGATTCCGAGCTTGATACGTTCCTCGGTGGCGTCCCCGATCATCTAACCGCAATTCCAAAGCACATAGTTGATGATCGCTTCCTCGAAACGGTCAACACCGATCCGCACACACAAGGACTaaa                                                                                                                                                                                                                                                                              >  2:609434/1‑289 (MQ=255)
                   tGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACca                                                                                                                                                                                                                                                                           <  1:617493/289‑1 (MQ=255)
                   tGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACca                                                                                                                                                                                                                                                                           <  1:506070/289‑1 (MQ=255)
                           tGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTc                                                                                                                                                                                                                                                                   >  2:186904/1‑289 (MQ=255)
                              aaCCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAg                                                                                                                                                                                                                                                                <  1:676211/289‑1 (MQ=255)
                               aCCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATCCGTTCTGCGGTGGCCTCACCGATCAGAGAACCGTACTTACGACGCCCAGCGTTGATGATAGCTTCGTCGAAACGGTCCCCACCAATGCGCCCAGAAGAGGCGCAAACCTCACCGTCCAAgg                                                                                                                                                                                                                                                               >  2:241816/1‑289 (MQ=255)
                               aCCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAgg                                                                                                                                                                                                                                                               >  2:446075/1‑289 (MQ=255)
                                   ttACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGAt                                                                                                                                                                                                                                                           <  2:684516/289‑1 (MQ=255)
                                   ttACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGAt                                                                                                                                                                                                                                                           <  2:91008/289‑1 (MQ=255)
                                        gcgcTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAg                                                                                                                                                                                                                                                      <  2:30210/289‑1 (MQ=255)
                                          gcTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGc                                                                                                                                                                                                                                                     <  1:186904/288‑1 (MQ=255)
                                           cTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGca                                                                                                                                                                                                                                                                                             <  2:427064/247‑1 (MQ=255)
                                           cTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGca                                                                                                                                                                                                                                                                                             >  1:427064/1‑247 (MQ=255)
                                           cTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCaa                                                                                                                                                                                                                                                   >  2:568497/1‑289 (MQ=255)
                                                    ccGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTgg                                                                                                                                                                                                                                          <  2:526970/289‑1 (MQ=255)
                                                      ggTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTa                                                                                                                                                                                                                                        >  2:13345/1‑289 (MQ=255)
                                                        tCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGCTAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAACAGGAGTAAACCACACCGTCCAAGGAGATAACAGCAAATTCAGTGGTaac                                                                                                                                                                                                                                      >  2:613648/1‑287 (MQ=255)
                                                        tCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTacc                                                                                                                                                                                                                                      >  2:329427/1‑289 (MQ=255)
                                                        tCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTacc                                                                                                                                                                                                                                      >  1:546972/1‑289 (MQ=255)
                                                        tCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTacc                                                                                                                                                                                                                                      >  1:217461/1‑289 (MQ=255)
                                                         aaGCGGTTCCTGCAGTGCTTCGAGGATTGCAATGGAGTTCAGGGGAGAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACAAACTTCGATATCACGGACTTCAAGGCCCGGATAAGCCGAACCGATTTCGTGCTTGCTACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGAACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTacc                                                                                                                                                                                                                                      <  2:672805/287‑1 (MQ=255)
                                                          aGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTacca                                                                                                                                                                                                                                     >  2:404612/1‑288 (MQ=255)
                                                            cGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTaccacc                                                                                                                                                                                                                                   >  1:222316/1‑288 (MQ=255)
                                                                    gCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATc                                                                                                                                                                                                                          <  1:568497/289‑1 (MQ=255)
                                                                       gtgctttggcgtgttCATTGGAGTTCAGGGTAAACCCGCGGGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACAGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAac                                                                                                                                                                                                                       <  2:489672/276‑1 (MQ=255)
                                                                        tgcgtcgaggtagtgaTTGGAGTTCAGGGAAGAACTGCGTGAAACACGCGATGTCAGTTGACGGCCACGGAAATCGAGTGAACGGGCTGCATAGCGCGAATAAGCCGAACCGGGTTCGTGAATGATACGTTCTGCGGTGGCTTAACCGATCAGAGAACCGTAATTACGACGCACATAGTTGAAGATAGATTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACTGTTCAAGGAGAGAACAGCATCGTCAGTGGTACAACCACCGATATCAac                                                                                                                                                                                                                       <  2:260423/273‑1 (MQ=255)
                                                                         gCTTCGCGGAGTACACTTGAGTTCAGAGGACAACCGCGTGGCAAAGCTTCTTGCAGGGTACAGCCACGAACTTCGATTTCACGGAATTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCAGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAac                                                                                                                                                                                                                       <  2:336225/287‑1 (MQ=255)
                                                                         gCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACcaca                                                                                                                                                                                                                                                                         >  1:313900/1‑237 (MQ=255)
                                                                         gCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACcaca                                                                                                                                                                                                                                                                         <  2:313900/237‑1 (MQ=255)
                                                                         gCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAac                                                                                                                                                                                                                       <  1:448570/287‑1 (MQ=255)
                                                                         gCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAac                                                                                                                                                                                                                       <  1:393768/287‑1 (MQ=255)
                                                                         gCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAac                                                                                                                                                                                                                       <  1:721670/287‑1 (MQ=255)
                                                                         gCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAac                                                                                                                                                                                                                       <  1:329427/287‑1 (MQ=255)
                                                                         gCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAac                                                                                                                                                                                                                       <  1:296565/287‑1 (MQ=255)
                                                                         gCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAac                                                                                                                                                                                                                       <  1:270259/287‑1 (MQ=255)
                                                                                                cAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACTGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCCCATAGTTGATGATAGCTTCGTCGAACCGGTAACCACCAATACGCACAGAAGAGCAGTAAACCAACCCGGTCAAGGAGATAACAGCAACTCCAGTGGTACCAACAACGATCTCAACCACACTAGAACCCGTccatcaagaa                                                                                                                                                                                              >  2:440882/1‑280 (MQ=255)
                                                                                                cAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACCGCAACTTCAGTGGTACCACCACCGATATCAACCACCATCGAACCGGTCCCTTCAGaa                                                                                                                                                                                              >  1:420425/1‑289 (MQ=255)
                                                                                                aaGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGAGACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGAACATAGTTGATGATAGCCTCGTCGAAACGGTCACCACAAATGCGCACAGAAGAGGAGTAAACAAAAACGGTCAAGGAGATCACCGCAACGCAAGTGGCACAACAACCGATATCaagcccactagaaacgtccacctcagaa                                                                                                                                                                                              >  2:440861/2‑263 (MQ=255)
                                                                                                     tAAAACCACGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACGTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCgg                                                                                                                                                                                         <  2:268605/289‑1 (MQ=255)
                                                                                                        aaCCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAac                                                                                                                                                                                                                       >  1:469146/1‑256 (MQ=255)
                                                                                                        aaCCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAac                                                                                                                                                                                                                       <  2:469146/256‑1 (MQ=255)
                                                                                                         aCCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAgg                                                                                                                                                                                     <  1:404612/289‑1 (MQ=255)
                                                                                                             cgTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAac                                                                                                                                                                                                                       <  1:170324/251‑1 (MQ=255)
                                                                                                             cgTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAac                                                                                                                                                                                                                       >  2:170324/1‑251 (MQ=255)
                                                                                                                  aaCACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCa                                                                                                                                                                            >  1:25904/1‑289 (MQ=255)
                                                                                                                         tCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGc                                                                                                                                                                                                                                                     <  1:396932/209‑1 (MQ=255)
                                                                                                                         tCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGc                                                                                                                                                                                                                                                     >  2:396932/1‑209 (MQ=255)
                                                                                                                            gCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACcaca                                                                                                                                                                                                                                                                         >  1:641984/1‑186 (MQ=255)
                                                                                                                            gCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACcaca                                                                                                                                                                                                                                                                         <  2:641984/186‑1 (MQ=255)
                                                                                                                                            gAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAAt                                                                                                                                                  <  1:446075/289‑1 (MQ=255)
                                                                                                                                                           ggACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACg                                                                                                                                   <  1:746587/289‑1 (MQ=255)
                                                                                                                                                              cgtCATCGCCCGGATAAGCCGAACCGATTTGGTGCTGGAGACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGACCATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACggg                                                                                                                                 <  2:488937/286‑1 (MQ=255)
                                                                                                                                                                            tAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGGGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTgcg                                                                                                                  <  2:289888/289‑1 (MQ=255)
                                                                                                                                                                                     ccGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCAc                                                                                                         >  1:597186/1‑289 (MQ=255)
                                                                                                                                                                                     ccGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCAc                                                                                                         >  2:395634/1‑289 (MQ=255)
                                                                                                                                                                                              tGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTgcgc                                                                                                >  1:214237/1‑289 (MQ=255)
                                                                                                                                                                                               gCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTgcgc                                                                                                <  1:13345/288‑1 (MQ=255)
                                                                                                                                                                                                          tCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCgg                                                                                                                                                                     >  2:95173/1‑208 (MQ=255)
                                                                                                                                                                                                          tCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCgg                                                                                                                                                                     <  1:95173/208‑1 (MQ=255)
                                                                                                                                                                                                              cGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGACGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCAGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGt                                                                                >  2:333225/1‑289 (MQ=255)
                                                                                                                                                                                                                             tCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAac                                                                                                                                                                                                                       >  1:515306/1‑139 (MQ=255)
                                                                                                                                                                                                                             tCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAac                                                                                                                                                                                                                       <  2:515306/139‑1 (MQ=255)
                                                                                                                                                                                                                             tCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGcac                                                                 >  1:422586/1‑289 (MQ=255)
                                                                                                                                                                                                                                      cGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAg                                                        >  2:627125/1‑289 (MQ=255)
                                                                                                                                                                                                                                        tAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCGATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGa                                                       >  1:397140/1‑288 (MQ=255)
                                                                                                                                                                                                                                        tAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGa                                                       >  1:272661/1‑288 (MQ=255)
                                                                                                                                                                                                                                            tACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCGCCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAcgcg                                                  <  2:445372/289‑1 (MQ=255)
                                                                                                                                                                                                                                            tACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAcgcg                                                  <  2:272661/289‑1 (MQ=255)
                                                                                                                                                                                                                                                               gatAGCTTCGTCGAAACGTTCACCACCAATGCGAACAGAAGAGGAGTAAACCAAACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACGGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTGCAATCAGGAAGAATTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAg                               <  1:440882/289‑1 (MQ=255)
                                                                                                                                                                                                                                                               gatAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAATAGGAGTAAACCACACCGTTCAAGAAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAg                               <  1:440861/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                  aGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCCTCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGACTTGCGCGGCGTTCATCCTGGGTCGCGCCAACCGGCACACACACCAGAACGCGCGGGCCTGGACGCATaga                                >  2:9449/1‑283 (MQ=255)
                                                                                                                                                                                                                                                                    cTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACTGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGCTCAACCTGGCTCGACCCAACCGGCGCACAAACCAGAACGCGGGGGCTTGGACGCCGACAGCTGtt                          >  2:454521/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                    cTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCCCACAAACCAGCACCCGCGGGCTTGGACGCATAAAGCGGtt                          >  2:688515/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                    cTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGtt                          >  1:8928/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                    cTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGtt                          >  1:366163/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                    cTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACAAAAACCAGAACGCGCGGGATTGGACGCATAAAGCTGtt                          >  1:360207/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                    cTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCCCACAAACCAAAACGCGCGGGCTTGGACGCATCAAGCTGtt                          >  2:551152/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                       cgtcgAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACAACACCGTTCATGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCAACAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGACACCCGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACCCGTGGGCTGGGACGAATAAAGATGTTgat                       >  2:426712/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                        gtcgAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGc                                                                                                                                                                                    >  2:458669/1‑131 (MQ=255)
                                                                                                                                                                                                                                                                        gtcgAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGc                                                                                                                                                                                    <  1:458669/131‑1 (MQ=255)
                                                                                                                                                                                                                                                                        gtcgAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCtg                      <  1:613648/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                            aaaCGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCa                  <  2:422586/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                             aaCGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCAc                 <  2:214237/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       accgATGCGCACAGAAAAGAGGTAAACCACACCGTTCAAAGAGATAACAGCAACTTTAGTGGTACCACAACCGAGATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAACACCAATTGCAGCAGCCAGCGGTTCTTCAATCAGGAAGACGTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGATCGCGCCAACCGGCACACAATCCTGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACGTGTTtgatg       <  2:597186/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       accaATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtgatg       <  1:695809/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                           aTGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGCTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGACACCCGGCACACAAACCAGAACGCGCGGGGTTGGACGCATAACGGTGTAGCTGTGCACTGGTTTGATGAAGt   >  2:752505/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                            tGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCCTAAAGCTGTTGCTGGTCACTTGTTTGATGAAGTg  >  2:695809/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                            tGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGACGTg  >  1:633134/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                            tGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGt   >  1:28852/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                            tGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGt   >  1:703766/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                             
AGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTG  >  NC_000913/3400237‑3400809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: