Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,402,648 A→G 3.3% V228A (GTG→GCG)  csrD ← targeting factor for csrBC sRNA degradation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,402,6480AG 3.3% 428.8 / 2.7 122V228A (GTG→GCG) csrDtargeting factor for csrBC sRNA degradation
Reads supporting (aligned to +/- strand):  ref base A (56/61);  new base G (2/2);  total (59/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

Only 100 of 122 total aligned reads displayed.

GCCTCTTTTAACGTCCGGTGCGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTACCGCCAGGAAGAGCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACG  >  NC_000913/3402369‑3402933
                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                             
gCCTCTTTTAACGTCCGGTGCGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCGACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTggg                                                                                                                                                                                                                                                                                      <  1:725040/289‑1 (MQ=255)
gCCTCTTTTAACGTCCGGTGCGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTggg                                                                                                                                                                                                                                                                                      <  1:173166/289‑1 (MQ=255)
     ttttAACGTCCGGTGCGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCa                                                                                                                                                                                                                                                                                 >  1:369409/1‑289 (MQ=255)
     ttttAACGTCCGGTGCGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCa                                                                                                                                                                                                                                                                                 >  1:286157/1‑289 (MQ=255)
      tttAACGTCCGGTGCGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCAt                                                                                                                                                                                                                                                                                <  1:210935/289‑1 (MQ=255)
      tttAACGTCCGGTGCGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCAt                                                                                                                                                                                                                                                                                <  2:464232/289‑1 (MQ=255)
       ttAACGTCCGGTGCGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCCGTGCGCCACGGTAGCGCATCCTACATCTCCACAGCAGCTTCGTCAGAGTGAAGCACTGTTCTTCAACCTGGCTCTGCCCCCAGGTATCGCTCAACCTATTGAAGTCCGGCAGACGACTCATCATCACGACCCCGCGGGTACCTACCTTCTCCTACTCTTCCAGTAACGTTGCTAACTGATTATCCACCACGAGTCGGTTATTGAGCCCGGCTTTCCCGTCCTGGGCCGCCTa                                                                                                                                                                                                                                                                               >  2:665788/1‑289 (MQ=255)
          aCGTCCGGTGCGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCTCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAaga                                                                                                                                                                                                                                                                            <  2:262166/289‑1 (MQ=255)
           cGTCCGCGGCGGTAACAGCGCACCAAAATCACGGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAagag                                                                                                                                                                                                                                                                           <  2:276608/289‑1 (MQ=255)
             tCCGGTGCGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGTGTCGGTTATTTAGGCCGGTTTTCACGTCCTGGGCGGTATAAGAGCg                                                                                                                                                                                                                                                                         >  2:368231/1‑289 (MQ=255)
               cGGTGCGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAagag                                                                                                                                                                                                                                                                           >  2:703901/1‑285 (MQ=255)
                            gcgcAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCAc                                                                                                                                                                                                                                                                                              >  1:134410/1‑253 (MQ=255)
                              gcggcaAAAGCCCGGCGGGGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAAGGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGa                                                                                                                                                                                                                                                         <  2:479624/285‑1 (MQ=255)
                              gcagcaAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGa                                                                                                                                                                                                                                                         <  1:88907/288‑1 (MQ=255)
                                          cTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGtgttgt                                                                                                                                                                                                                                             >  1:583381/1‑288 (MQ=255)
                                            gCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTc                                                                                                                                                                                                                                           >  1:249917/1‑288 (MQ=255)
                                               gtggtAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCgcgt                                                                                                                                                                                                                                       >  2:682871/1‑289 (MQ=255)
                                                       gcgcCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTg                                                                                                                                                                                                                               >  1:95622/1‑289 (MQ=255)
                                                         gcCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGaa                                                                                                                                                                                                                             <  2:369409/289‑1 (MQ=255)
                                                            agcagTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCGCGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAAtt                                                                                                                                                                                                                           >  1:515648/1‑288 (MQ=255)
                                                                 tGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGa                                                                                                                                                                                                                     >  1:220966/1‑289 (MQ=255)
                                                                 tGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTGCACGTCCTGGGCGGCATAAGAGCGGAACAGCGTATCAAGCCTGCTGGTTTGTTCGCGTGCGTTCGGAATTTCagca                                                                                                                                                                                                                     >  2:470426/1‑286 (MQ=255)
                                                                  gcgcCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACg                                                                                                                                                                                                                      <  1:368231/287‑1 (MQ=255)
                                                                   cgcagggctAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCCGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAAACCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGaa                                                                                                                                                                                                                    <  1:388463/280‑1 (MQ=255)
                                                                   cgcCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGGTGTTCGCGTGCGTTCTGAATTTccagaa                                                                                                                                                                                                                    >  2:719509/1‑283 (MQ=255)
                                                                        gggTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCg                                                                                                                                                                                                              >  1:725747/1‑289 (MQ=255)
                                                                         ggTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGt                                                                                                                                                                                                             >  2:462210/1‑289 (MQ=255)
                                                                              cgcATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCa                                                                                                                                                                                                        >  1:96652/1‑289 (MQ=255)
                                                                                   catAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTggg                                                                                                                                                                                                                                                                                      <  1:281392/206‑1 (MQ=255)
                                                                                   catAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTggg                                                                                                                                                                                                                                                                                      >  2:281392/1‑206 (MQ=255)
                                                                                     tAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAgcgc                                                                                                                                                                                                    >  1:81276/1‑286 (MQ=255)
                                                                                      aaaTGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCActg                                                                                                                                                                                                <  1:639884/289‑1 (MQ=255)
                                                                                       aaTGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCActgc                                                                                                                                                                                               <  2:583381/289‑1 (MQ=255)
                                                                                        aTGTCGACAGCAGCTTCGTCTGAGTGACGAACTGTTCTTCAACCTGGCTGTGCCCTCAGGTACCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCCCGATCCCGCGGGTACCTACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTCTCGAGGCAGGTTTTCACGTCCAGGGCGGCATCAGACCGGATTCGCGTTACAAGCCGGCCGTGTGGTTCGGGTCCCTTCTGAATTTCACGAAGCCACGTCTCCAggcaccgt                                                                                                                                                                                                >  2:383264/1‑280 (MQ=255)
                                                                                             gACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAACAGCGTCGGTTATTGAGTCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGACCAGCGTATCAAGCCGGCTGTGTTGTTCGCGTGCGGTCTGAATTTCACGACGAAGCGTATCCCGCGCTCTGCTGGt                                                                                                                                                                                           >  2:447791/1‑287 (MQ=255)
                                                                                             gACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCGCGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGGGTGCGTTCTGAATTTCCCGAAGCAGCGTTACCAGCCCCATGCTGGttt                                                                                                                                                                                         >  2:144833/1‑288 (MQ=255)
                                                                                             gACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCAt                                                                                                                                                                                                                                                                                >  1:650341/1‑202 (MQ=255)
                                                                                             gACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGa                                                                                                                                                                                                                                                                       <  2:206813/211‑1 (MQ=255)
                                                                                             gACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGa                                                                                                                                                                                                                                                                       >  1:206813/1‑211 (MQ=255)
                                                                                                     aTTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGaa                                                                                                                                                                                                                    >  1:275193/1‑254 (MQ=255)
                                                                                                     aTTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGaa                                                                                                                                                                                                                    <  2:275193/254‑1 (MQ=255)
                                                                                                      ttCGTCAGAGTGAGAAAGTGTACTTCAACCAGGCGGTGCCCCCAGGTATCGCGCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGggcggc                                                                                                                                                                                 <  2:300847/288‑1 (MQ=255)
                                                                                                      ttCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGggcgg                                                                                                                                                                                  <  1:94811/287‑1 (MQ=255)
                                                                                                       tCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCa                                                                                                                                                                               <  1:719509/289‑1 (MQ=255)
                                                                                                              agTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAg                                                                                                                                                                        >  2:181534/1‑289 (MQ=255)
                                                                                                                tgaagaaaggttgtgagagcTGGCTGTGCCGCCGGGGATCGTTCAACAGATTGGAATCCGGCAGGCGAATCATCATCACGATCCTCTGGGTACCTACATTCTCCTGATCGTCCAGTAACGTTGCTAACTGATTATCGAAAAAGCGTCGGGTATGGAGGCCGGTTTTCACGTCCTGAGCGGCATAAGAGCGGATCAGCGTGTGAAGACGGCTGTGTTGTTCGCGTGCGCTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGGTCTGGGGGGCCATTCATAGa                                                                                                                                                                       <  2:88654/269‑1 (MQ=255)
                                                                                                                tGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATGGAAATCCGCCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGa                                                                                                                                                                       <  2:218651/288‑1 (MQ=255)
                                                                                                                      aCTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTcc                                                                                                                                                                <  2:81276/289‑1 (MQ=255)
                                                                                                                         ggtcttcAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCaa                                                                                                                                                             <  2:444488/287‑1 (MQ=255)
                                                                                                                                    tGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACCGCTTGTTCTGGGCGGCCTTTCATAGATGGTTCCCAATCCATTagag                                                                                                                                                   >  1:278910/1‑288 (MQ=255)
                                                                                                                                       cTGTGCCCCCAAGTATCGCGCAACATATTGGAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCGTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTGTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCa                                                                                                                                                <  2:635451/288‑1 (MQ=255)
                                                                                                                                           gCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCt                                                                                                                                            <  1:665788/288‑1 (MQ=255)
                                                                                                                                            cccccAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCaa                                                                                                                                                             >  1:690032/1‑270 (MQ=255)
                                                                                                                                            cccccAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCt                                                                                                                                            <  2:96375/287‑1 (MQ=255)
                                                                                                                                            cccccAGGGATCGCTCAACAGATTGAAATCCGGCAGACGATGCATCATCACGATCCCGAGGGTAACTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCa                                                                                                                                          <  2:220966/289‑1 (MQ=255)
                                                                                                                                             ccccAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCa                                                                                                                                          <  1:682871/288‑1 (MQ=255)
                                                                                                                                                    tATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTTCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCCTTCATAGATGGTTCCAAACACATTAGAGCCACGCTCCCCGTTTaa                                                                                                                                  >  2:660292/1‑289 (MQ=255)
                                                                                                                                                    tATCGCTCAACATATTGAAAACCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGttt                                                                                                                                    <  2:249917/287‑1 (MQ=255)
                                                                                                                                                      tCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGa                                                                                                                                                                                                                                                         >  2:232473/1‑168 (MQ=255)
                                                                                                                                                      tCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGa                                                                                                                                                                                                                                                         <  1:232473/168‑1 (MQ=255)
                                                                                                                                                         cTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACg                                                                                                                                              >  2:370234/1‑272 (MQ=255)
                                                                                                                                                         cTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACg                                                                                                                                              <  1:370234/272‑1 (MQ=255)
                                                                                                                                                            aaCATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGat                                                                                                                          >  1:417147/1‑288 (MQ=255)
                                                                                                                                                            aaCATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAg                                                                                                                          <  2:594560/289‑1 (MQ=255)
                                                                                                                                                                        tCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGtt                                                                                                               >  1:336672/1‑288 (MQ=255)
                                                                                                                                                                            gCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCa                                                                                                           >  1:694629/1‑288 (MQ=255)
                                                                                                                                                                                               gATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGaa                                                                                                                                                                                                                    <  2:76652/164‑1 (MQ=255)
                                                                                                                                                                                               gATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGaa                                                                                                                                                                                                                    >  1:76652/1‑164 (MQ=255)
                                                                                                                                                                                                 tCCCTTGGGTACCTCCGTTCTCCTGATCTTCCAGTAACGTTGCTACCTGATTATCGAAAAAGAGTCGGTTATTGGGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGGATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGtt                                                                                      <  2:68415/288‑1 (MQ=255)
                                                                                                                                                                                                 tCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTg                                                                                     <  1:451149/289‑1 (MQ=255)
                                                                                                                                                                                                 tCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTCGGCGGCCATTCATAGATGGTTCCCAACACCTTAGAGCCACCCACCCCGTTTAAATTACGAGTACGCAGGGTTTGCAGCAATTCTTCACCGCCAAGtt                                                                                      >  2:147821/1‑288 (MQ=255)
                                                                                                                                                                                                   ccGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTGGCTAACGGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTg                                                                                     <  2:364766/287‑1 (MQ=255)
                                                                                                                                                                                                    cGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGggcg                                                                                                                                                                                   >  1:482422/1‑192 (MQ=255)
                                                                                                                                                                                                    cGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGggcg                                                                                                                                                                                   <  2:482422/192‑1 (MQ=255)
                                                                                                                                                                                                    cGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCg                                                                                  >  1:519699/1‑289 (MQ=255)
                                                                                                                                                                                                    cGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCg                                                                                  >  1:68415/1‑289 (MQ=255)
                                                                                                                                                                                                      tGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGggcggc                                                                                                                                                                                 <  1:524853/192‑1 (MQ=255)
                                                                                                                                                                                                      tGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGggcggc                                                                                                                                                                                 >  2:524853/1‑192 (MQ=255)
                                                                                                                                                                                                           aCCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTaa                                                                            >  1:610808/1‑288 (MQ=255)
                                                                                                                                                                                                           aCCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAAc                                                                           <  2:725747/289‑1 (MQ=255)
                                                                                                                                                                                                                    ctcCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTg                                                                                     <  2:400820/270‑1 (MQ=255)
                                                                                                                                                                                                                    ctcCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTg                                                                                     >  1:400820/1‑270 (MQ=255)
                                                                                                                                                                                                                          aTCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTACCGCCAgg                                                            >  2:565532/1‑289 (MQ=255)
                                                                                                                                                                                                                            cTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCGCGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTACCGCCAGGaa                                                          <  2:515648/289‑1 (MQ=255)
                                                                                                                                                                                                                                      cGTTGTTAACTGATTATCGAAAAAGAGTCGGTTATTGGTGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTACCGCCAGGAAGAGCATAACa                                                <  2:417147/289‑1 (MQ=255)
                                                                                                                                                                                                                                         tGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGggcggc                                                                                                                                                                                 <  1:438823/157‑1 (MQ=255)
                                                                                                                                                                                                                                         tGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGggcggc                                                                                                                                                                                 >  2:438823/1‑157 (MQ=255)
                                                                                                                                                                                                                                         tGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCt                                                                                                                                            >  1:398776/1‑194 (MQ=255)
                                                                                                                                                                                                                                         tGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCt                                                                                                                                            <  2:398776/194‑1 (MQ=255)
                                                                                                                                                                                                                                            tAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCGCGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTACCGCCAGGAAGAGCATAACAATGAt                                           <  1:144833/288‑1 (MQ=255)
                                                                                                                                                                                                                                            tAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTACCGCCAGGAAGAGCATAACAATGAt                                           <  1:447791/288‑1 (MQ=255)
                                                                                                                                                                                                                                               cTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTACCGCCAGGAAGAGCATAACAATGATAAAg                                       <  2:694629/289‑1 (MQ=255)
                                                                                                                                                                                                                                                  aTTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTACCGCCAGGAAGAGCATAACAATGATAAAGCCa                                    >  1:622906/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                       aTTGAGGCCGGTTTTAACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTACCGCCAGGAAGAGCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGcgcg               <  1:131230/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                               cGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCa                                                                                                                                          <  1:382174/158‑1 (MQ=255)
                                                                                                                                                                                                                                                                               cGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCg                                                                      >  1:153585/1‑226 (MQ=255)
                                                                                                                                                                                                                                                                               cGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCg                                                                      <  2:153585/226‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  ttttCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTACCGCCAGGAAGAGCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGcgcg               <  1:737144/278‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  ttttCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTACCGCCAGGAAGAGCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGcgcg               >  2:737144/1‑278 (MQ=255)
                                                                                                                                                                                                                                                                                    ttCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTACCGCCAGGAAGAGCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACg  <  1:462210/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                             
GCCTCTTTTAACGTCCGGTGCGGTAACAGCGCAGCAAAATCACTGCGGTGGTAACGCGCCAGCAGTGCGCCAGGGTAGCGCATCATAAATGTCGACAGCAGATTCGTCAGAGTGAAGAACTGTTCTTCAACCTGGCTGTGCCCCCAGGTATCGCTCAACATATTGAAATCCGGCAGACGAATCATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTACCGCCAGGAAGAGCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACG  >  NC_000913/3402369‑3402933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: