Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	59 [0]	[0] 60	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	54 (1.330)	45/274	0.0	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

ACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT  >  NC_000913/1300560‑1300840                                                                                                                                           |                                                                                                                                               acagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataat                                                                                                                                            <  1:253203‑M2/5‑1 (MQ=255)     ttagttataaggatttccttaactgcttctcctcaccatcatattattttcgccacatcataatcgtgggcttgctgaagaataattgacatgatattattaattccgctgcctttggtaaaggaaagtgctaaATAATAATc                                                                                                                                        >  1:505160‑M2/135‑143 (MQ=255)           attaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGt                                                                                                                                  <  1:485887‑M2/15‑1 (MQ=255)           ataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGt                                                                                                                                  >  2:707561‑M2/129‑143 (MQ=255)           ataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaaaaataattgaaatgatattattaattccactgccttagatagaggaaagtgctaaATAATAATCAATTGt                                                                                                                                  >  1:343658‑M2/129‑143 (MQ=255)                gatttccttaactgattttaattacaaacaggatattttcgccaaatcatgattctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAat                                                                                                                             >  2:732477‑M2/124‑143 (MQ=255)                 atttccttaactgcttctcctcaccatcatgttattttcgctacatcataatcctgggcttgatgaagaataattgaaatgatattattaattcaattgactttggtagaggaaagtgataaATAATAATAAATTGTTAAatt                                                                                                                            >  1:202458‑M2/123‑143 (MQ=255)                      cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgctactactacttccactgactttggtagaggaaagtgctaaATCATAATCACTTGTTCAATCATtgt                                                                                                                       >  1:183087‑M2/118‑143 (MQ=255)                      cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattcctctgcctttggtagcggaaagtgctaaATAATAATCACTTGTTAAATTATtgt                                                                                                                       >  2:305023‑M2/118‑143 (MQ=255)                      cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaaattatttt                                                                                                                       >  2:696167‑M2/118‑141 (MQ=255)                          actgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAt                                                                                                                   <  2:730243‑M2/30‑1 (MQ=255)                              cttctcctccccatcatgttattttcgccacatcataatcctaggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCa                                                                                                               <  2:7839‑M2/34‑1 (MQ=255)                              cttctcctcaccatcatgttattttcgccacatcataatcctgcgcttgctgaagaataattaaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCa                                                                                                               <  1:49026‑M2/34‑1 (MQ=255)                                tctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCact                                                                                                             <  2:575044‑M2/36‑1 (MQ=255)                                 ctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCacta                                                                                                            <  1:36515‑M2/37‑1 (MQ=255)                                   cctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaagtccactgcctttggtagaggaaagtgctaaATAATAAACAATCGTTAAATTATTGCACACTTAactact                                                                                                          >  1:542303‑M2/105‑143 (MQ=255)                                       tgcatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGaa                                                                                                      <  1:107454‑M2/43‑1 (MQ=255)                                         catcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactacctgtgatagaggtaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACt                                                                                                    >  2:338227‑M2/99‑143 (MQ=255)                                            catgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTACTGTGCATTTCAATACTAGAACCGTa                                                                                                 >  1:475028‑M2/96‑143 (MQ=255)                                              tgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAt                                                                                               <  1:231346‑M2/50‑1 (MQ=255)                                                  attttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGa                                                                                           >  1:633596‑M2/90‑143 (MQ=255)                                                   ttttcgccacatcataatcctgggctggctgaagaataattgaaatgatattattgattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaa                                                                                          <  1:110278‑M2/55‑1 (MQ=255)                                                     ttcgccacatcataatgctgggcttgctgaagaataattgaaaggatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaaa                                                                                        <  1:542552‑M2/57‑1 (MQ=255)                                                     ttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaaa                                                                                        <  2:678393‑M2/57‑1 (MQ=255)                                                     ttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaaa                                                                                        >  1:375181‑M2/87‑143 (MQ=255)                                                         ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATTAGAAAAGATa                                                                                    >  1:413852‑M2/83‑143 (MQ=255)                                                         ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATa                                                                                    >  2:711881‑M2/83‑143 (MQ=255)                                                         ccacatcataagcctgggctggctgaaggataattgaaatgaaattagtaatcccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATa                                                                                    <  2:306281‑M2/61‑1 (MQ=255)                                                          cacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAg                                                                                   >  2:510360‑M2/82‑143 (MQ=255)                                                           acatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGa                                                                                  >  2:428205‑M2/81‑143 (MQ=255)                                                                 taatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCt                                                                            >  2:274365‑M2/75‑143 (MQ=255)                                                                      ctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTATcc                                                                       >  2:224177‑M2/70‑143 (MQ=255)                                                                           cttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAtct                                                                  >  1:423158‑M2/65‑143 (MQ=255)                                                                                 gaagaataattgaaatgatattattaattccaccgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAttt                                                            >  1:89685‑M2/59‑143 (MQ=255)                                                                                   agaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttga                                                          <  2:353071‑M2/87‑1 (MQ=255)                                                                                      ataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgattg                                                       <  1:466033‑M2/90‑1 (MQ=255)                                                                                        aattgaaatgatattattaattccactgcctgtggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaa                                                     <  2:157482‑M2/92‑1 (MQ=255)                                                                                              aatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACGTGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                               <  1:626385‑M2/98‑1 (MQ=255)                                                                                                    attattaattccactgcctttggtagaggaaagtgctaaATAATAATCACTTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGACTTGAAAGAtgtt                                         >  2:468535‑M2/40‑143 (MQ=255)                                                                                                    attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtt                                         <  2:265522‑M2/104‑1 (MQ=255)                                                                                                     ttattaattccactgccgttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                        <  1:168397‑M2/105‑1 (MQ=255)                                                                                                     ttattaagtccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                        <  2:317591‑M2/105‑1 (MQ=255)                                                                                                        tgaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgtt                                     >  2:538885‑M2/36‑143 (MQ=255)                                                                                                           attccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtt                                         <  1:360093‑M2/104‑1 (MQ=255)                                                                                                           attccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtt                                         >  2:360093‑M2/33‑136 (MQ=255)                                                                                                               cactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCAt                              <  2:633596‑M2/115‑1 (MQ=255)                                                                                                                  tgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGa                           >  2:573182‑M2/26‑143 (MQ=255)                                                                                                                     ctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGc                        >  2:18015‑M2/23‑143 (MQ=255)                                                                                                                       ttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCaa                      >  2:112113‑M2/21‑143 (MQ=255)                                                                                                                          gtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCt                   <  2:573348‑M2/126‑1 (MQ=255)                                                                                                                                gaaagtgctaaATAATAATCAATTGTTAAAGTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATCGACATGCTTAGCCAATCGCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGAt             >  1:431889‑M2/12‑143 (MQ=255)                                                                                                                                  aagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTc           <  2:899‑M2/134‑1 (MQ=255)                                                                                                                                   agtgctaaATAAGAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCt          <  1:198550‑M2/135‑1 (MQ=255)                                                                                                                                      gctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGAt       <  1:558301‑M2/138‑1 (MQ=255)                                                                                                                                       ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATa      <  1:607248‑M2/139‑1 (MQ=255)                                                                                                                                        taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGGTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATAc     >  2:47240‑M2/4‑143 (MQ=255)                                                                                                                                        taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGAAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATAc     >  1:628516‑M2/4‑143 (MQ=255)                                                                                                                                        taaATAATAATCAATGGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGCTGCAAGCTATAGATTCTGATAc     >  1:617517‑M2/4‑143 (MQ=255)                                                                                                                                         aaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACg    <  2:510513‑M2/141‑1 (MQ=255)                                                                                                                                           ataataatCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGt  <  1:726892/143‑1 (MQ=255)                                                                                                                                           |                                                                                                                                               ACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT  >  NC_000913/1300560‑1300840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑